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1.
The survival of the coliform bacterium Enterobacter aerogenes in seawater was greatly affected by the underlying bottom sediments. The presence of sediments which contained higher concentrations of organic nutrients than the overlaying seawater prolonged the survival and even enabled growth of the bacterial cells. Results obtained from chemical analysis indicated that sediments of fine-grained, silt-clay type contained highest amounts of organic nutrients as compared with sediments with larger particle sizes such as silt and sand grains. Thus, nutrient contents of the sediments were found to be inversely proportional to sediment particle size. The degree of prolonged survival of E. aerogenes was also found to be inversely proportional to the size of the sediment particles presented. Release of the bound nutrients which became available to cells of E. aerogenes from sediments by action of waves and human activities can explain, at least in part, why high counts of coliform bacteria are obtained in coastal and beach waters.  相似文献   

2.
Environmental variables and their association with faecal coliform and streptococci densities were investigated at a number of Sydney's metropolitan beaches. High variability in faecal coliform and faecal streptococci densities was observed at most beaches. The ability of the various models to explain the observed variation in faecal coliform or streptococci levels was relatively low, although they did provide an insight into the more influential environmental factors involved. Rainfall has the single most important effect on faecal coliform and streptococci densities at Sydney beaches. Whether this is due to increased stormwater runoff at the beaches, increased discharge through the outfalls (both the deepwater ocean outfalls and the few remaining shoreline outfalls), or a combination of both is unclear and is likely to depend on the individual beach being considered. Elevated levels of faecal coliforms and streptococci are still occasionally observed at some beaches, but these levels are usually not as high nor as frequent as they were prior to commissioning of the deepwater outfalls. The significant associations of current and plume entrapment with faecal coliform and streptococci densities at some Sydney beaches suggest that under certain conditions, effluent from the deepwater ocean outfalls can still influence faecal coliform and streptococci densities at these beaches.  相似文献   

3.
Levels of bacterial indicators of pollution are related with marine salinity and turbidity at both high tide (HT) and low tide (LT). The salinity varied from values around 26.9 ppm at the LT and 28.6 ppm at the high tide but affected total and faecal coliform (FC) estimates. Salinity readings of 25–30 ppm produced microbial counts below 10−2 MPN/100 ml total coliforms (TCs) whereas salinity of 15–22 ppm produced a TC level of 4.6×10−4 MPN/100 ml. Turbidity peaks in the samples are accompanied by peaks of microbial contamination of the seawater indicating that the contamination is normally deposited at the marine sediment rather than in the water column. In fact, samples collected under heavy stormy weather, in which the water agitation resulted in turbidity values up to 68.3 NTU, produced maximum microbial counts.  相似文献   

4.
Long Island Sound was divided with a total of 25 north-south transects. Ninety-six stations were established along these transects at which sediment and/or water and plankton samples were collected. Total faecal coliforms and total viable bacteria were enumerated from sediments from 53 stations. The presence of vibrios was determined in sediment, water and plankton samples from 186 enrichments.

Counts obtained along the Connecticut shore were similar to those along the Long Island shore. No correlation was noted between the prevalence of faecal coliforms and total viable bacteria in sediment from the same station. Dilute seawater medium was superior to Marine Agar (Difco) for the enumeration of bacteria from 34 of the 53 stations tested. Presumptive vibrios were present in 95% of the sediment samples, 51% of the water samples and 100% of the plankton samples. No correlation was observed between the distribution of vibrios and faecal coliforms.  相似文献   


5.
The power of total coliforms (TC), faecal coliforms (FC) and Enterococci to predict the presence of Salmonella in seawater was investigated. Indicator cut-off values with the most satisfactory combination of sensitivity and specificity in predicting Salmonella presence were 1000 CFU 100 ml−1 TC, 200 CFU 100 ml−1 FC, 500 CFU 100 ml−1 Enterococci. When TC or FC were used for Salmonella prediction in logistic regression, then the addition of another indicator did not have a statistically significant effect. When Enterococci were used for prediction, then the addition of either of the two other indicators led to a statistically significant improvement (P = 0.001 for TC, P = 0.003 for FC). These results suggest that using either TC or FC alone provided an adequate indicator of Salmonella presence, but a statistically significant improvement is possible over using Enterococci alone. Concerning Enterococci, European Union limits for excellent coastal water quality (100 CFU 100 ml−1) and United States Environmental Protection Agency criteria for marine bathing waters (35 CFU 100 ml−1) have the same value in predicting Salmonella absence (92.5%).  相似文献   

6.
Contamination of Drinking Water with Coliform Organisms by the Larves of Gnats in the Water Treatment Plant E. coli and coliform organisms are used to judge the microbial quality of drinking water. The presence of these organisms in water indicates that there is a risk for faecal oral transmitted pathogen organisms. E. coli shows the faecal contamination of men and warm blooded animals (mammalian and birds), coliform organisms may be excreted as well from cold blooded animals. The cold blooded animals normally don’t excrete pathogen organisms relevant for human beings. So coliform organisms don’t have such a high relevance for drinking water microbiology. In this paper an incident is presented with a contamination of drinking water with coliform organisms in the treatment plant by the larves of gnats. The larves of the gnats crawl into the treatment chamber via entrance for ventilation. The larves were kept back on the surface of the filters but they contaminated the water with coliform organisms.  相似文献   

7.
The toxicity of marine sediments in Victoria Harbour, Hong Kong   总被引：4，自引：0，他引：4  
When the toxicity of marine sediment in Hong Kong was evaluated, it was found that the seven sediments collected within Victoria Harbour were severely contaminated with heavy metals, at concentrations many times higher than those in sediments collected from outside the harbour. The highest metal content was recorded in site VS14 (located near the airport runway and the industrialized area), with copper, zinc, lead and chromium values of 3789, 610, 138 and 601 mg kg−1 dry wt, respectively. This site also had the greatest alkaline phosphatase activities (15 fluorescent intensity unit g−1 wet wt), the largest number of total coliforms (910 CFU g−1 wet wt) and sulphate-reducing bacteria (8.5 × 104 cells g−1 wet wt), implying that site VS14 was also contaminated with organic matter and nutrients. Sediment bioassays, Microtox and algal tests, demonstrated that sediment elutriates obtained from site VS14 were of greatest toxicity. The EC10 value in Microtox tests was 17% elutriate, and the 96-h IC50 values using Skeletonema costatum and Dunaliella tertiolecta were 40 and 79% elutriate, respectively. No toxic effects were found in sediment samples collected from the control site outside Victoria Harbour. Significant correlations were found between the results of the algal toxicity test (using S. costatum) and the coliform count and metal content of the sediments. The Microtox test was less sensitive than the algal bioassay, and no sediment elutriate, even from the site mostly contaminated by heavy metals, caused more than 50% inhibition of the light-emitting activity of the bacteria. In this study, S. costatum (the diatom) provided a more sensitive and reliable test species than D. tertiolecta (the flagellate) in differentiating the toxicity of marine sediments.  相似文献   

8.
We report new data on oxygen isotopes in marine sulfate (δ18OSO4), measured in marine barite (BaSO4), over the Cenozoic. The δ18OSO4 varies by 6‰ over the Cenozoic, with major peaks 3, 15, 30 and 55 Ma. The δ18OSO4 does not co-vary with the δ34SSO4, emphasizing that different processes control the oxygen and sulfur isotopic composition of sulfate. This indicates that temporal changes in the δ18OSO4 over the Cenozoic must reflect changes in the isotopic fractionation associated with the sulfide reoxidation pathway. This suggests that variations in the aerial extent of different types of organic-rich sediments may have a significant impact on the biogeochemical sulfur cycle and emphasizes that the sulfur cycle is less sensitive to net organic carbon burial than to changes in the conditions of that organic carbon burial. The δ18OSO4 also does not co-vary with the δ18O measured in benthic foraminifera, emphasizing that oxygen isotopes in water and sulfate remain out of equilibrium over the lifetime of sulfate in the ocean. A simple box model was used to explore dynamics of the marine sulfur cycle with respect to both oxygen and sulfur isotopes over the Cenozoic. We interpret variability in the δ18OSO4 to reflect changes in the aerial distribution of conditions within organic-rich sediments, from periods with more localized, organic-rich sediments, to periods with more diffuse organic carbon burial. While these changes may not impact the net organic carbon burial, they will greatly affect the way that sulfur is processed within organic-rich sediments, impacting the sulfide reoxidation pathway and thus the δ18OSO4. Our qualitative interpretation of the record suggests that sulfate concentrations were probably lower earlier in the Cenozoic.  相似文献   

9.
The deltaic estuarine system of the Matang Mangrove Forest Reserve of Malaysia is a site where several human settlements and brackish water aquaculture have been established. Here, we evaluated the level of fecal indicator bacteria (FIB) and the presence of potentially pathogenic bacteria in the surface water and sediments. Higher levels of FIB were detected at downstream sampling sites from the fishing village, indicating it as a possible source of anthropogenic pollution to the estuary. Enterococci levels in the estuarine sediments were higher than in the surface water, while total coliforms and E. coli in the estuarine sediments were not detected in all samples. Also, various types of potentially pathogenic bacteria, including Klebsiella pneumoniae, Serratia marcescens and Enterobacter cloacae were isolated. The results indicate that the Matang estuarine system is contaminated with various types of potential human bacterial pathogens which might pose a health risk to the public.  相似文献   

10.
In many smallholder farms in sub-Saharan Africa dambos are used for grazing and crop production especially horticultural crops. Increased use of dambos especially for crop production can result in ground and surface water pollution. Ground and surface water quality along a dambo transect in Chihota, Zimbabwe, was investigated between October 2013 and February 2014. The transect was divided into; upland (control), dambo gardens (mid-slope) and the river (valley bottom). Water samples for quality assessment were collected in October 2013 (peak of dry season) and February 2014 (peak of rainy season). The collected water samples were analysed for pH, faecal coliforms, total nitrogen, electrical conductivity, total dissolved solids (TDS), and some selected nutrients (P, K, Ca, Mg, Na, Zn, and Cu). Water pH was 7.0, 6.4 and 6.1 for river water, garden and upland wells respectively. During the wet season total nitrogen (TN) concentrations were 233 mg/L for uplands, 242 mg/L for gardens and 141 mg/L for the river. During the dry season, TN concentrations were all below 20 mg/L, and were not significantly different among sampling stations along the dambo transect. Dry season faecal coliform units (fcu) were significantly different and were 37.2, 30.0 and 5.0 for upland wells, garden wells and river respectively. Wet season faecal coliforms were also significantly different and were 428.5, 258.0 and 479.4 fcu for upland wells, garden wells and river respectively. The other measured physico-chemical parameters also varied with sampling position along the transect. It was concluded that TN and fcu in sampled water varied with season and that wet season concentrations were significantly higher than dry season concentrations. High concentrations of faecal coliforms and total N during the wet season was attributed to increased water movement. Water from upland wells, garden wells and river was not suitable for human consumption according to WHO standards during both the dry and wet seasons.  相似文献   

11.
《Marine pollution bulletin》2009,58(6-12):403-408
Laboratory experiments were carried out to investigate the adsorption behaviour of dibutyl phthalate (DBP) on marine sediments collected from five different sites in Victoria Harbour, Hong Kong. DBP adsorption can be well described by the Langmuir isotherm. The maximum DBP adsorption capacity (Qmax) of the marine sediments ranges from 53 to 79 mg g−1, which has a positive correlation with their organic content. Around 90% of the organic can be removed from the sediments with treatment by H2O2 oxidation, and the Qmax then decreases to a range between 13 and 22 mg g−1. The black carbon content of the sediments has a much greater DBP adsorption capacity than does the natural organic matter of the sediments. The amount of DBP adsorbed on the sediments increases as the salinity of the marine water increases.  相似文献   

12.
5β-Stanols, and in particular coprostanol, which is one of the principal sterols of human sewage, show promise as chemical tracers of faecal pollution in estuaries. In the present survey, coprostanol has been measured gas chromatographically in the waters and sediments of the Clyde Estuary. Thus, the bottom sediments contained 0.1–14 ppm of coprostanol (5β-cholestan-3β-ol) together with the corresponding 24-methyl- and 24-ethyl-5β-stanols, while in the seawater samples, the coprostanol concentrations closely paralled the faecal coliform counts. Overall, the amounts of coprostanol correlate well with the location and amounts of the known inputs of sewage from outfalls and dumping.  相似文献   

13.
Untreated sewage has been released from Port-aux Fran?ais station, Kerguelen Island, into the Southern Ocean for more than 50 years. We investigated the spatial distribution of faecal bacteria indicators during a one-year survey conducted in seawater off Morbihan Bay near the French permanent station of the Kerguelen Island (49 degrees 21(')S, 70 degrees 30(')E). Seawater samples were taken bimonthly from nine stations evenly distributed around the sewage outfalls of the station. Escherichia coli and enterococci were estimated using specific microplates (Miniaturized method for the enumeration of E. coli or enterococci in surface and waste waters, "MU/EC or MU/SF methods", BIO-RAD( Copyright)). In order to evaluate the role of seasonal changes of environmental parameters on the survival of enteric bacteria, total and saprophytic bacterial abundances were also estimated in all seawater samples. High densities of faecal bacteria (maximum 10(4) cells 100 ml(-1)) were found in seawater surrounding the sewage outfall. However, enteric bacterial counts decreased rapidly with increasing distance from the outfall. In all samples collected further than 2 km from the outfall, the bacterial indicators were absent or present in small numbers (<10 cells 100 ml(-1)). Faecal coliforms were not detected in samples collected at pristine sites located 10 km from Port-aux-Fran?ais. Despite these low contamination levels, faecal bacteria were always detected in the vicinity of the sewage outfall during the seasonal survey. The concentration of faecal bacteria may be related to the number of people inhabiting the station.  相似文献   

14.
The influence of riverine inputs on biogeochemical cycling and organic matter recycling in sediments on the continental shelf off the Rhône River mouth (NW Mediterranean Sea) was investigated by measuring sediment oxygen uptake rates using a combination of in situ and laboratory techniques. Four stations were investigated during two cruises in June 2001 and June 2002, with depths ranging from 9 to 192 m and over a distance to the Rhône River mouth ranging from 4 to 36 km. Diffusive oxygen uptake (DOU) rates were determined using an in situ sediment microprofiler and total oxygen uptake (TOU) rates were measured using sediment core incubations. There was good agreement between these two techniques which indicates that the non-diffusive fraction of the oxygen flux was minimal at the investigated stations. DOU rates ranged from 3.7±0.4 mmol O2 m−2 d−1 at the continental shelf break to 19.3±0.5 mmol O2 m−2 d−1 in front of the Rhône River mouth. Sediment oxygen uptake rates mostly decreased with increasing depth and with distance from the Rhône mouth. The highest oxygen uptake rate was observed at 63 m on the Rhône prodelta, corresponding to intense remineralization of organic matter. This oxygen uptake rate was much larger than expected for the increasing bathymetry, which indicates that biogeochemical cycles and benthic deposition are largely influenced by the Rhône River inputs. This functioning was also supported by the detailed spatial distribution of total organic carbon (TOC), total nitrogen (TN) and C/N atomic ratio in surficial sediments. Sediments of the Rhône prodelta are enriched in organic carbon (2–2.2%) relative to the continental shelf sediments (<1%) and showed C/N ratios exceeding Redfield stoichiometry for fresh marine organic matter. A positive exponential correlation was found between DOU and TOC contents (r2=0.98, n=4). South-westward of the Rhône River mouth, sediments contained highly degraded organic matter of both terrestrial and marine origin, due to direct inputs from the Rhône River, sedimentation of marine organic matter and organic material redeposition after resuspension events.  相似文献   

15.
The sorption behavior of nonylphenol (NP, a toxic endocrine disruptor) on marine sediments was studied in detail through a series of kinetic and thermodynamic sorption experiments. The results showed that the sorption reaction of NP on marine sediments reached equilibrium in 1.5 h and that it accorded well with the non-linear Ho-McKay pseudo-second-order model. The sorption isotherms of NP on H2O-treated sediments could be well described by the Linear isotherm model, while the sorption isotherm on H2O2-treated sediments could be well fitted with the Freundlich isotherm model. A positive correlation was found between the distribution coefficient (Kd) and the sediment organic carbon contents. The medium salinity showed a positive relation with the Kd and a negative relation with the dissolved organic carbon (DOC). Hexadecyl trimethyl ammonium bromide (CTAB) enhanced the sorption amount of NP the most, while sodium dodecylbenzenesulfonate (SDBS) enhanced it the least. The sorption reaction of NP on marine sediments was a spontaneous, physical, exothermic and entropy-decreasing process.  相似文献   

16.
With colony numbers of 2 · 104… 3 · 105 and coliform contents of 2 · 103… 1 · 1 · 104 per ml, in the investigated river section 2.1% of colonies were resistant to tetracycline, 0.3% to chloramphenicol, 7.5% to kanamycin, 5.4% to trimethoprim, 12.7% to ampicillin and 0.14% to gentamycin. For the coliforms the resistances were 5.1% to tetracycline, 2.1% to chloramphenicol, 2.7% to kanamycin and 0.15% to gentamycin. There were isolated 63 different resistance types. Of the resistant Klebsiella strains 75% were resistant to three or more antibiotics. Of the coliform bacteria (Escherichia coli, Klebsiella, Enterobacter, Citrobacter) 31.6… 51.9% of the strains transferred their antibiotics-resistance to Escherichia coli K-12, for the antibiotics-resistant strains of Pseudomonas and Aeromonas a transfer of resistance was not observed at all.  相似文献   

17.
The study aimed to determine the spatial distribution of n-alkanes and polycyclic aromatic hydrocarbons (PAHs) in surface sediments of the Segara Anakan nature reserve and their potential origins using gas chromatography–mass spectrometry. Total alkane concentrations ranged from 3755 to 129,027 μg kg−1, and the concentrations of 16 PAHs ranged from 375 to 29,517 μg kg−1. The ratios of specific n-alkanes (e.g., CPI24–34, WaxCn, and Paq), including a new proposed index, terrestrial–marine discriminant (TMD), as well as the ratios of selected PAHs (e.g., Ant/∑178, Fl/∑202, BaAnt/∑228, and IPyr/∑276), showed that the sources of hydrocarbons in the sediments were generally biogenic, including both terrigenous and marine, with an anthropogenic pyrolytic contribution (petrogenic and biogenic combustion). For the environmental risk assessment, a sediment quality guideline (SQGs) comparison indicated that the station risk levels ranged from low to medium-low, except for R6, which has a greater impact on the ecological risk for marine organisms.  相似文献   

18.
The majority of coastal Antarctic stations release untreated sewage into the near-shore marine environment. This study examined bacterial reproduction within the temporary sewage-holding tanks of Rothera Research Station (Adelaide Island, Antarctic Peninsula) and monitored sewage pollution in the local marine environment. By continuously flushing the sewage-holding tanks with cold seawater we inhibited microbial reproduction and decreased the numbers of bacteria subsequently released into the sea by >90%. The widespread use of this simple method could significantly reduce the numbers of faecal coliform and other non-native microorganisms introduced into the Antarctic marine environment.  相似文献   

19.
Deposit-feeding holothurians are important processors of surface sediments in many coastal marine systems. The present study examined the effect of grazing by the sea cucumber Australostichopus mollis on sediment impacted by green-lipped mussel biodeposits (faeces and pseudofaeces) from coastal aquaculture activities. Grazing effects were investigated in a series of tank-based feeding experiments conducted over 1, 2, 4 and 8 week periods. Sediment quality indicators routinely applied to determine the impacts of coastal aquaculture were used to evaluate sediment health from grazed and ungrazed sediments. Sea cucumber grazing resulted in reductions in total organic carbon, chlorophyll a and phaeopigment, as well as chlorophyll a/phaeopigment ratio of impacted sediments. These results demonstrate that sea cucumber grazing significantly reduces the accumulation of both organic carbon and phytopigments associated with biodeposition from mussel farms. Sea cucumber grazing offers a means of constraining or reversing the pollutive impacts of coastal bivalve aquaculture.  相似文献   

20.
228Ra concentrations were measured in 12 subsurface water samples collected during August–September 1970 in the North Atlantic at the Second Geosecs Intercalibration Station.228Ra concentrations ranged from 1.5 to 2.1 dpm/100 kg in the water layer between 100 and 600 m depth, above the main thermocline, but decreased with increasing depth to less than 0.5 dpm/100 kg at 1.5 km, below the main thermocline. The two deepest samples, collected below 4 km depth, indicated that the228Ra concentration increased again to at least 1.2 dpm/100 kg as the interface with the sea floor sediments was approached. Above the 4 km level, the228Ra profile was roughly similar to the tritium profile measured by Roether and Münnich.  相似文献   

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