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1.
Four enzymes and one protein were tested in starch and cellulose acetate gels as biochemical tags for the separation of flounder (Rhombosolea spp.) larvae and O‐groups. Lactate dehydrogenase phenotypes separated O‐groups and larvae (longer than about 5 mm) of R. plebeia from R. leporina and R. retiaria. General protein phenotypes separated similar specimens of R. leporina from R. plebeia and R. retiaria. Most Rhombosolea larvae and O‐groups caught in Wellington Harbour were R. plebeia.  相似文献   

2.
Vesicomyid clams, vestimentiferans, and some bathymodiolin mussels from hydrothermal vents and cold seeps possess thiotrophic endosymbionts, high levels of hypotaurine and, in tissues with symbionts, thiotaurine. The latter, a product of hypotaurine and sulfide, may store and/or transport sulfide non‐toxically, and the ratio to hypotaurine plus thiotaurine (Th/[H + Th]) may reflect an animal's sulfide exposure. To test this, we analyzed seep and vent animals with in situ sulfide measurements. Calyptogena kilmeri clams occur at high‐sulfide seeps in Monterey Canyon, while C. (Vesicomya) pacifica clams occur at seeps with lower levels but take up and metabolize sulfide more effectively. From one seep where they co‐occur, both had gill thiotaurine contents at 22–25 mmol kg−1 wet mass, and while C. (V.) pacifica had a higher blood sulfide level, it had a lower Th/[H + Th] (0.39) than C. kilmeri (0.63). However, these same species from different seeps with lower sulfide exposures had lower ratios. Bathymodiolus thermophilus [East Pacific Rise (EPR 9°50′ N)] from high‐(84 μm ) and a low‐(7 μm ) sulfide vents had gill ratios of 0.40 and 0.12, respectively. Trophosomes of Riftia pachyptila (EPR 9°50′ N) from medium‐(33 μm ) and low‐(4 μm ) sulfide vents had ratios of 0.23 and 0.20, respectively (not significantly different). Ridgeia piscesae vestimentiferans (Juan de Fuca Ridge) have very different phenotypes at high‐ and low‐sulfide sites, and their trophosomes had the greatest differences: 0.81 and 0.04 ratios from high‐ and low‐sulfide sites, respectively. Thus Th/[H + Th] may indicate sulfide exposure levels within species, but not in interspecies comparisons, possibly due to phylogenetic and metabolic differences. Total H + Th was constant within each species (except in R. piscesae); the sum may indicate the maximum potential sulfide load that a species faces.  相似文献   

3.
We examined the response of four species of New Zealand marine algae (Ecklonia radiata, Apophlaea lyallii, Rhodymenia spp., Ulva lactuca) and a sea urchin (Evechinus chloroticus) to spatial variation in ultraviolet radiation (UV‐R) by examining the concentration of UV‐R absorbing compounds known as mycosporine‐like amino acids (MAAs). The purpose was to understand how, and the degree to which, local marine species could potentially respond to any future increases in incident UV‐R in the New Zealand marine environment. The research was undertaken in Doubtful Sound, where we observed a gradient of water column UV‐R transmission along the 40 km length of the fiord. We examined spatial differences in MAAs along the UV‐B gradient in the macrophytes and temporal changes in MAAs in sea urchin gonads. Among the algae, thallus MAA concentrations (nmol mg–1 protein) ranged from 12.5 to 87.8 in E. radiata, from 433.1 to 1446.4 in A. lyallii, 12.7 to 103.4 in Rhodymenia spp., but were not detected in U. lactuca. For E. chloroticus, gonadal MAA concentrations ranged from 83.9 to 224.3 nmol mg–1 protein spatially, and over the year from 1.85 to 14.12 nmol mg–1 dry weight (DW) depending on site and gametogenic cycle. Laboratory manipulations indicated that concentrations of MAAs in E. chloroticus gonads and eggs are influenced by diet. MAA concentration could be correlated with UV‐B intensities in two of the algal species. E. chloroticus MAA concentrations could also be correlated with UV‐B transmission, which we concluded was a reflection of the greater ingestion and accumulation of MAA‐rich macrophytes at those sites where higher ambient UV‐R induced greater MAA concentrations to occur in the algae. Given this, we suggest that one response of marine species to increases in UV‐B would be an increase in the synthesis and/or accumulation of MAAs for photoautotrophs and a dietary accumulation of those MAAs in E. chloroticus, an important herbivore in this system.  相似文献   

4.
The variation in mycosporine‐like amino acid (MAA) concentration in the soft coral Heteroxenia fuscescens in relation to changes in ultraviolet radiation (UVR) regimes was investigated at the Gulf of Eilat, northern Red Sea. Solar radiation (300–700 nm) was measured for different depths and seasons. The UVR irradiance was measured to a depth of 25 m on the reef. The mean attenuation coefficient for UV‐B measured in winter was twofold that of the summer value. Separation of H. fuscescens extracts by reverse‐phase isocratic high‐performance liquid chromatography revealed a single MAA compound, palythine (λmax = 320 nm). Possible seasonal changes in MAAs in colonies of H. fuscescens along a depth gradient were examined on different dates. Palythine concentrations in the colonies were significantly higher in summer than in the other seasons particularly in shallow water. Possible changes in MAA content in colonies of H. fuscescens as a result of UVR protection, were determined by experiments conducted for periods of 1 week, 1 month and 3 months, at a depth of 5 m. In these experiments colonies were removed from the natural substrate and placed underwater, protected from UVR by a PVC filter. Significant differences between UV‐exposed and protected colonies of H. fuscescens were found only in the 3‐month experiment conducted during the summer. These findings demonstrate that UVR is an important environmental factor regulating MAA biosynthesis in the soft coral H. fuscescens.  相似文献   

5.
Growth rates of three species of abalone in New Zealand were estimated by fitting data to the von Bertalanffy growth equation. Length‐frequency analysis and tagging were used for Haliotis iris Martyn, and shell growth‐checks were used for H. australis Gmelin and H. virginea Gmelin. The relative growth coefficient (K) and the asymptotic length (L8) were found to be 0.3104 and 146.2 mm respectively for H. iris, 0.3205 and 86.75 mm for H. australis, 0.4460 and 61.5 mm, and 0.3231 and 64.4 mm for H. virginea from two separate localities. An absolute growth curve could be calculated only for Haliotis iris.  相似文献   

6.
This taxon is distinguished from other Ergasilidae by having only three pairs of legs, and a well‐developed hook on the penultimate joint of the second antenna abutting against the claw‐like terminal segment. A. amplexus has been reported from Anguilla australis, A. dieffenbachii, Perca fluviatilis, Carassius auratus, Retropinna retropinna, Physiculus bachus, Galaxias maculatus, Arripis trutta, Rhombosolea leporina, R. retiaria, and R. plebeia from Lake Ellesmere, near Christchurch, and from Anguilla australis from Makara River near Wellington. Both localities are euryhaline.  相似文献   

7.
Habitat created or modified by the physical architecture of large or spatially dominant species plays an important role in structuring communities in a variety of terrestrial, aquatic, and marine habitats. At hydrothermal vents, the giant tubeworm Riftia pachyptila forms large and dense aggregations in a spatially and temporally variable environment. The density and diversity of smaller invertebrates is higher in association with aggregations of R. pachyptila than on the surrounding basalt rock seafloor. Artificial substrata designed to mimic R. pachyptila aggregations were deployed along a gradient of productivity to test the hypothesis that high local species diversity is maintained by the provision of complex physical structure in areas of diffuse hydrothermal flow. After 1 year, species assemblages were compared among artificial aggregations in low‐, intermediate‐, and high‐productivity zones and compared to natural aggregations of R. pachyptila from the same site. Hydrothermal vent fauna colonized every artificial aggregation, and both epifaunal density and species richness were highest in areas of high chemosynthetic primary production. The species richness was also similar between natural aggregations of R. pachyptila and artificial aggregations in intermediate‐ and high‐productivity zones, suggesting that complex physical structure alone can support local species diversity in areas of chemosynthetic primary production. Differences in the community composition between natural and artificial aggregations reflect the variability in microhabitat conditions and biological interactions associated with hydrothermal fluid flux at low‐temperature hydrothermal vents. Moreover, these local ecological factors may further contribute to the maintenance of regional species diversity in hydrothermal vent communities on the East Pacific Rise.  相似文献   

8.
Book review     
Nga tini a Tangaroa, a Maori‐English, English‐Maori dictionary offish names. By R. R. Strickland. New Zealand fisheries occasional publication 5. 1990. 64 unnumbered pages, ISSN 0113–227X. Available from Primedia Bookshop, P.O. Box 2526, Wellington, New Zealand. Price: $26.95.  相似文献   

9.
Terminal‐restriction fragment length polymorphisms (T‐RFLPs) were used to address the question whether there are multiple phylotypes of bacteria within the trophosome of the vestimentiferan tubeworm Ridgeia piscesae in addition to the known endosymbiont. Clone libraries were constructed to aid in the identification of the additional bacterial phylotypes. Individual R. piscesae specimens were collected from Juan de Fuca Ridge, Axial Caldera and Explorer Ridge, Magic Mountain. Clone library analyses revealed only one bacterial phylotype (expected R. piscesae endosymbiont) within the Explorer Ridge trophosomes. However, the Axial Caldera clone library revealed five operational taxonomic units (OTUs). Three of the resulting phylotypes detected (designated RAE) were putative thioautotrophic symbionts within the γProteobacteria which belonged to the anticipated endosymbiont group (RAE OTU 1), the Maorithyas hadalis symbiont II group (RAE OTU 3), and the Halothiobacillus group (RAE OTU 4). The remaining two phylotypes were most likely opportunistically derived. RAE OTU 5 was an αProteobacterium within the Roseobacter group and RAE OTU 2 was within the Cytophaga–Flavobacterium–Bacteroidetes. T‐RFLP analyses revealed that all 15 trophosomes evaluated contained at least RAE OTUs 1 and 4. Five of the 15 trophosomes contained at least RAE OTUs 1, 3, and 4 and two of the trophosomes contained at least five RAE OTUs. Cluster analysis of the T‐RFLP data revealed three distinct clusters. The number of taxa within the trophosome may be an indication of the general health of the tubeworm. This study strongly suggests that we have discovered and identified more than one bacterial phylotype within the trophosome of vestimentiferan R. piscesae.  相似文献   

10.
11.
Rhodopirellula baltica is a ubiquitous Planctomycetes organism isolated from marine environments worldwide. Multilocus sequence analysis (MLSA) has been the only method used to assess the diversity of R. baltica organisms. Here, we developed multispacer sequence typing (MST) genotyping and applied it to four R. baltica isolates from the French Mediterranean Sea and R. baltica DSM10527T type strain. While MLSA yielded interpretable sequences for two of nine genes in three R. baltica isolates, MST based on sequencing two intergenic spacers yielded an unambiguous, strain‐specific profile for each one of the four R. baltica isolates under investigation. We propose that MST could be used as an alternative R. baltica genotyping method to assess the diversity of these Planctomycetes organisms.  相似文献   

12.
Tag‐recapture studies revealed differences in the growth rate of Haliotis iris between headlands and bays separated by as little as 200 m. Individuals off headlands had a significantly higher incremental growth and reached a higher maximum size than those in bays. These results were consistent with observations of the size composition of H. iris which showed that few individuals of harvestable size (> 125 mm shell length) were found in bays. Differences in the growth rate of H. iris may account for the apparent differences in the size at onset of maturity: individuals off headlands, and from a fast‐growing population off Wellington, reached reproductive maturity at a smaller size compared with those in bays. Yield‐per‐recruit modelling showed greater yields from headlands than bays, but egg‐per‐recruit modelling revealed more egg production was conserved in bays than off headlands. Yields could be increased from bays by decreasing the minimum size to 110 mm. Egg‐ and yield‐per‐recruit models were sensitive to the natural mortality rate (M = 0.03, 0.05 and 0.10 compared). The results show that parameters important in the management of commercial fisheries for abalone can vary over small distances: such variation should be incorporated in management strategies for abalone fisheries.  相似文献   

13.
We found that tandem repeat (TR) arrays were present in the ark shell Scapharca broughtonii mitochondrial genome, and we demonstrated the efficiency of using this TR region as a tool for the detection of genetic differentiation. In samples collected from seven populations off the Japanese, Chinese, Russian and Korean coasts, five TR types with length ranging from 481 to 601 bp (base pairs) were found, as were 18 haplotypes (n = 155). All the Japanese samples from SD, IK, AM and KG, which represented four geographically distinct populations, had the same type of tandem repeat array, 481‐TR. All the samples from China and all but one sample from Russia had the same TR type, 511‐TR; these finding may be strong evidence that the ark shell populations in China and Russia are very similar. Based on a neighbor‐joining dendrogram of haplotypes, the Korean population was divided into two groups as follows: samples with haplotype H481‐3 and samples with haplotypes H601‐1, H601‐2, H601‐3 or H541‐1. Based on the haplotype diversity and nucleotide diversity, the Chinese, Russian and Korean populations showed a higher diversity than Japanese populations (P < 0.05).  相似文献   

14.
Criteria based on external characters are presented for aging and sexing the three New Zealand species of oystercatcher, Haematopus ostralegus finschi Martens, 1897, H. unicolor Forster, 1844, and H. chathamensis Hartert, 1927. Four classes are discerned: juveniles have brown dorsal plumage, a brown iris and grey legs; second‐year birds have an orange‐red iris and pale pink legs; sub‐adults have a dull red iris and pink legs; adults have a scarlet iris and bright coral pink legs. The three species can be sexed by discriminant analysis of the sexually dimorphic characters bill length (x 1), bill length:bill depth (x 2) and bill length:bill width (x 3). Linear functions and discriminating values for predicting sex are :

H. ostralegus finschi 0.46x 1+3.15x 2+2.94x 3, 77.41;

H. unicolor 0.12x1+6.52x2+2.85x3 , 58.05; and

H. chathamemis ‐0.93x 1+1.50x 2+7.48x 3, ‐20.86.

Similarly, the sexes of immature H. ostralegus finschi can be predicted :

juveniles 0.73x1+ 5.76x 2 + 3.10 x3 116.57;

second‐year birds 0.57x 1+5.12x 2+ 0.98x 8, 84.84; and

sub‐adults 0.55x 1+1.88x 2+ 1.08x 3, 65.90.’  相似文献   

15.
A systematic assessment of Planctomycetales diversity in a South China Sea, deep‐sea sediment (1657 m) was conducted using the 16S rRNA gene analysis approach. PCR amplification of the samples from seven sediment layers (0.1, 1, 3, 5, 7, 9 and 11 m below the surface sediment) using the primer set Pla‐46‐F/1392‐R showed that the Planctomycetales existed within a limited range of sediment depths (≤ 5 m), and had a decreasing trend in diversity with increasing depth. The majority of the retrieved Pla‐46‐F/1392‐R sequences belonged to Pirellula‐related Planctomycetales, and two sequences retrieved from the 0.1‐m layer (GenBank accession numbers: DQ996944 and DQ996945 ) shared the same anammox‐related signature oligonucleotides and were closely related to commonly recognized anammox organisms. To identify new anammox‐related biomarkers, three primer sets were designed for amplifying the fragments of hydroxylamine oxidoreductase and S‐adenosylmethionine radical enzyme genes, but no related sequences were found. Our multiple 16S rRNA gene primer sets (Journal of Rapid Methods and Automation in Microbiology, 2008, in press) revealed even an higher diversity of Planctomycetales in the 0.1‐m layer of the sediment, especially at genus level. Our data profiled the distribution pattern of Planctomycetales diversity along sediment depths, and provided molecular evidence for the existence of anammox‐related bacteria in a new location, which broadens our understanding of Planctomycetales diversity in deep sea sediments.  相似文献   

16.
Haplognathia ruberrima is a cosmopolitan gnathostomulid species found in sulfur bacterial mats in mangroves in Guadeloupe (French West Indies). Haplognathia ruberrima presents a δ13C value lower than all measured meiofaunal grazers and lower than the available measured food sources of this environment. This low δ13C value can not be due to specific ingestion of 13C‐depleted methanogenic bacteria because abundances of those bacteria are reduced in surficial and deep sediments as revealed by δ13C of bacterial fatty acid. According to scanning electron microscope observations, no bacterial ectosymbionts were observed at the surface of the gnathostomulids, and transmission electron microscope views revealed the absence of bacterial endosymbionts. Energy‐dispersive X‐ray spectroscopy analysis detected low levels of sulfur (0.32%±0.8) in biological tissues of H. ruberrima, confirming the absence of thioautotrophic bacterial symbionts in these animals. Consequently, the low δ13C value of H. ruberrima can not be due to the presence of sulfur‐oxidizing symbionts but more probably to the selective and exclusive consumption of free‐living, sulfur‐oxidizing bacteria.  相似文献   

17.
Oceanography, Units 1–16 edited by J. Stewart (Open University Press, 1977), 755 pp.

The Thermohaline Fine Structure of the Ocean by K. N. Fedorov (New York: Pergamon Press, Pergamon Marine Series. Volume 2, 1978). Translated from Russian by D. A. Brown, Technical Editor: J. S. Turner, 170 pp., no price listed.

The Ocean Basins and Margins edited by Alan E. M. Nairn and Francis G. Stehli, with William H. Kanes (New York: Plenum Press, Vol. 1–4A, 4B, 1973, 1979, 1975, 1977, and 1978) approx. 3,000 pp.

Recent Crustal Movements, 1977 edited by C.A. Whitten, R. Green, and B.K. Meade (New York: Elsevier Scientific Publishing Co., 1979), 663 pp., hardcover, $78.00.

The Alaskan Shelf‐Hydrographic, Sedimentary, and Geotechnical Environment by Ghanshyam Datt Sharma (New York: Springer Verlag, 1979), 498 pp., hardcover, $29.80.  相似文献   

18.
Re: Kirby, D. S.; Abraham, E. R.; Uddstrom, M. J.; Dean, H. 2003: Tuna schools/aggregations in surface longline data 1993–98. New Zealand Journal of Marine and Freshwater Research 37: 633–644.  相似文献   

19.
Details are given of gut‐content analyses for nine fish species from the Avon‐Heathcote Estuary, Christchurch, New Zealand: sand flounder, Rhombosolea plebeia (Richardson); yellow‐bellied flounder, R. leporina (Hutton); common sole, Peltorhamphus novaezeelandiae (Gunther); yellow‐eyed mullet, Aldrichetta forsteri (Cuvier & Valenciennes); kahawai, Arripis trutta (Bloch & Schneider); spotty, Pseudolabrus celidotus (Bloch and Schneider); cockabully, Tripterygion nigripenne (Cuvier & Valenciennes); common bully, Gobiomorphus basalis (Gray); and globefish, Spheroides richei (Freminville). The percent occurrences of each food type recorded over Ihe sampling period (April 1965‐April 1966) for each species are compared. Monthly food tables are given for those species of which suitably large samples were obtained (sand flounder, yellow‐bellied flounder, common sole, yellow‐eyed mullet, and globefish). Where possible, the dietary occurrence of different food types is related to environmental and other factors observed or considered likely to influence food selection.  相似文献   

20.
为比较短蛸(Octopus ocellatus)对不同生物饵料的利用效果,以南美白对虾、肉球近方蟹、菲律宾蛤仔和玉筋鱼四种饵料进行了饲喂实验。结果表明:1)肉球近方蟹组短蛸增重率显著高于其他各组(P<0.05),南美白对虾组脏体比显著高于玉筋鱼组(P<0.05),各饵料组肝体比无显著差异;2)南美白对虾组和肉球近方蟹组短蛸肌肉的蛋白质含量显著高于菲律宾蛤仔组和玉筋鱼组(P<0.05),肉球近方蟹组脂肪含量显著高于其余各组(P<0.05),玉筋鱼组灰分含量显著低于其他各组(P<0.05);3)南美白对虾组短蛸肝胰腺谷丙转氨酶活性显著高于其余各组(P<0.05),肉球近方蟹组谷草转氨酶活性显著高于其余各组(P<0.05),玉筋鱼组谷氨酸脱氢酶活性显著低于其余各组(P<0.05),菲律宾蛤仔组酸性磷酸酶活性显著高于其余各组(P<0.05),玉筋鱼组胃蛋白酶活性显著高于其他各组(P<0.05)。综上,投喂肉球近方蟹可以显著提高短蛸的增重率;不同饵料对短蛸肌肉的蛋白质、脂肪和灰分含量有影响,对其肝胰腺功能和胃蛋白酶活性也有显著影响。  相似文献   

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