共查询到19条相似文献，搜索用时 281 毫秒
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用8种保存方法对淡水和海水水样中四个氮磷营养盐（NH3-N、NO3—N、NO3-N、PO4—P）参数及总氮的稳定性进行了7d保存效果的比较研究。结果表明，淡水与海水氮磷营养盐的最佳保存方法与效果各不相同，但淡水和海水的总氮值在各种方法保存下均十分恒定。综合考虑有效性及可操作性后提出了5d内的短期保存技术：（1）海水水样用5‰氯仿4℃条件下保存，供NH3-N、NO2—N和N03-N三个营养盐的测定，用5‰甲醛4℃条件下保存供PO4-P测定；（2）淡水用5‰氯仿4℃条件下保存，供NH3-N测定，5‰甲醛4℃条件保存供NO2-N、NO3-N和PO4-P测定。 相似文献
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三门湾夏季浮游植物现存量和初级生产力 总被引：1，自引：0，他引：1
该海区的生物、化学、水文等项目进行调查。用HQM-1型有机玻璃采水器采集水样,大面观测站仅采集表层(0～1m)水样,用于测定叶绿素a质量浓度和光合作用速率。在N2和N11两个连续观测站,每隔3h采集表层和5m层的水样,用于分析叶绿素a质量浓度及营养盐浓度。同时,用浅 型浮游生物网由近底层向表层垂直拖网采集浮游植物样品,所采样品被装入容积为600cm3的塑料瓶中,并加入20cm3的福尔马林溶液固定保存,供对浮游植物细胞计数和种属鉴定之用。营养盐样品用经酸预清洗过的孔径为0.45μm的醋酸纤维膜过滤,滤液用饱和HgCl2溶液固定后,低温避光保存。图… 相似文献
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水库水质监测是水库水质评价的重要依据,通常需要现场采水样后带回实验室进行检测与分析。然而一些特殊情况下,例如库区无船、情况复杂等,只能获得沿岸水样,导致水质监测结果无法代表水库整体水质质量。本文针对水库的水样离岸采集,基于自研发的无人船系统,设计集成了采水泵,并利用无人船位点跟踪技术,实现了远距离定点遥控采水样功能。在青岛棘洪滩水库,利用该系统,设置了两个采样点(离岸50 m和离岸1300 m),并成功获取了水样。在实验室内,利用分光光度计、离子色谱仪、电感耦合等离子体质谱仪等设备,完成了所采集水样的水质分析。实验结果表明,无人船应用于水库水样离岸采集具有可行性、实用性。 相似文献
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舟山近海环境DNA保存方法的建立及优化 总被引：1，自引：0，他引：1
近年来,环境DNA(environmentalDNA,eDNA)技术开始被广泛应用于水生生物多样性研究。本文通过绝对定量和高通量测序技术对舟山近海高浊度水样eDNA的保存方法进行了建立和优化。研究结果如下:(1)"酒精+低温"保存法的eDNA获得量是"酒精+常温"保存法的0.78—1.06 (7d)、0.89—1.80 (15d)、1.05—2.75 (30d)和2.69 (60d)倍;(2)酒精保存法(低温、常温)存在eDNA富集物渗漏及滤膜黏附现象;(3)短期内冷冻保存样品的eDNA获得量是酒精保存样品获得量的1.25—1.59倍(7d)和1.07—1.20倍(15d);(4)酒精保存法的eDNA降解速率慢于冷冻保存法,长期内酒精保存样品的eDNA获得量是冷冻保存样品获得量的1.99—2.10倍(30d)和2.84—7.64倍(60d);(5)二次富集(过滤)能显著提高eDNA获得量,富集组eDNA浓度是未富集组浓度的1.60—4.95倍("酒精+低温")和1.21—2.04倍("酒精+常温");(6)"酒精+低温"保存的样品在高通量测序总丰度、各鱼种分丰度、物种多样性指数等多个方面优于冷冻保存样品;(7)微生物(micro-organism)的eDNA降解速率慢于大生物(macro-organism),不同界(Kingdom)的eDNA最优保存方法可能并不相同。本研究首次建立了舟山近海高浊度水样eDNA最适保存方法,为相似水域的eDNA保存提供了借鉴参考。 相似文献
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为了适应我国近海海洋环境监测快速、多要素的现场监测需求,研制了一套船载水样自动采集系统,系统控制方式简单灵活,具有全自动、半自动和手动三种控制模式。该系统由采水泵、复合采水缆、电动绞车、电控柜、储水罐等硬件和控制软件组成。在软件的控制下,绞车、采水泵等执行元件可自动开启和关闭,自动完成同一地点不同深度的多层水样采集。与传统人工采水方式相比,可大大减轻实验人员的劳动强度,减小水样采集的深度误差。渤海和东海大量的海上试验证明该系统性能稳定,能够适用于营养盐分析仪对水样的要求。 相似文献
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Freezing as a method of sample preservation for the analysis of dissolved inorganic nutrients in seawater 总被引：1，自引：0，他引：1
John E. Dore Terrence Houlihan Dale V. Hebel Georgia Tien Luis Tupas David M. Karl 《Marine Chemistry》1996,53(3-4)
It is often desirable or necessary to store collected seawater samples prior to analysis for dissolved inorganic nutrients. It is therefore important to establish preservation and storage techniques that will ensure sample integrity and will not alter the precision or accuracy of analysis. We have performed a series of experiments on the storage of nutrient samples collected at the oligotrophic North Pacific benchmark Station ALOHA, using both standard autoanalyses and low-level techniques. Our results reveal that for oligotrophic oceanic waters, the immediate freezing of an unfiltered water sample in a clean polyethylene bottle is a suitable preservation method. This procedure is simple, it avoids potentially contaminating sample manipulations and chemical additions, and it adequately preserves the concentrations of nitrate + nitrite, soluble reactive phosphate, and soluble reactive silicate within a single water sample. 相似文献
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Accurate and precise measurements of stable lead isotopic compositions in seawater are critically dependent upon the ultra-clean techniques developed for accurate measurements of low (0.1 ng kg−1) lead concentrations in seawater by thermal ionization mass spectrometry. These include both limiting and measuring lead contamination during all phases of sampling, storage and analysis. Essential to this are samplers and shipboard facilities for collecting uncontaminated samples, and laboratory facilities for cleaning containers, purifying reagents and processing samples and standards. Techniques are also required to compensate for the absence of an internal standard, refractory hydrocarbon contaminants and the limited sensitivity of the detectors. Adaptation of those techniques has lowered the lead analytical detection limit for seawater analyses to 0.02 ng kg−1. While the precision of these analyses (0.1–0.4%) is less than that of high precision isotope ratio measurements (0.001%), it is sufficient to identify sources of lead inputs to the ocean and to trace biogeochemical cycles of lead within the ocean. The accuracy of these initial data is currently being established with interlaboratory calibrations and evaluated in terms of its oceanographic consistency. 相似文献
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多波束测深系统的精度评估方法研究 总被引：2，自引：1，他引：2
在分析了多波束测深系统测量误差来源的基础上，讨论了多波柬测深系统静态精度、相对精度和绝对精度的系统精度评估方法。采用的静态精度评估方法就是在多波柬测深系统静止的条件下考核其对同一位置测量深度的误差；相对精度评估方法就是布设多条交叉重叠的测线，考核交叉重叠点的测深误差；绝对精度评估方法是在多波束测深的同时利用高精度的测深仪测量同一区域，用此参考地理模型来检验多波束测深的精度。根据误差理论，三种精度评估的方法分别从系统稳定性、自符合性和系统误差方面确定各误差源的综合误差，它们是检验多波束测深系统精度是否符合海道测量标准的有效方法。文中给出了系统试验数据的重要结果及设备验收的方法。 相似文献
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Jacco Kromkamp 《新西兰海洋与淡水研究杂志》2013,47(3):457-465
The most common storage products of cyanobacteria are polyphosphate as a phosphorus storage compound, cyanophycin or phycobilin protein pigment as nitrogen storage products, and glycogen as a storage product of both carbon and energy. Nutrient uptake kinetics are regulated by the storage pools, and the patterns of regulation have a feedback effect on the amount of accumulated nutrient in the cells. Besides having a storage function the nutrient storage products are likely to act as metabolic sinks during conditions of energy stress. Regulation of storage products is especially strict in light‐limited cultures. By increasing the rate of polysaccharide formation during growth with short photoperiods, cyanobacteria are able to sustain relatively high growth rates. This effect is enhanced by keeping respiratory losses very low. 相似文献
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