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1.
Adult Palaemonetes pugio were collected from two tidal creek systems, Piles Creek (PC), a mercury polluted estuary, and Big Sheepshead Creek (BSC), a relatively pristine creek. Adult killifish (Fundulus heteroclitus), a natural predator of P. pugio, were obtained from BSC. For each test, ten treated (0·01 mg/liter mercuric chloride (HgCl), or 0·01 mg/liter methylmercuric chloride (MeHg)), or control shrimp here introduced into a tank containing three fish. The time between capture of the first and second BSC HgCl treated shrimp was significantly faster (P < 0·05), as was the time between the first and second capture (P < 0·05) of MeHg treated BSC shrimp when compared with their respective controls. In addition, significantly more (P < 0·025) BSC HgCl treated shrimp were captured after 120 min. No significant difference existed between control and HgCl treated PC shrimp; however, significantly more PC MeHg treated shrimp were captured after 60 (P < 0·05) min when compared with their respective controls. These data suggest that PC shrimp, subjected to mercury in their natural environment, are more tolerant to the sublethal effects of both HgCl and MeHg. These data also suggest that behavioral studies can be very sensitive assays for determining the effects of sublethal concentrations of toxicants on populations of organisms.  相似文献   

2.
During a two-week period, Rangia rapidly accumulates mercury (as mercuric chloride) from dilute solution (0·03–0·05 ppm) with the majority being located in gill and mantle tissue. Initial depuration (5 h) in clean seawater is rapid in all tissues. From 10–192 h concentrations of mercury in gill, foot and adductor muscles remain stable while mantle, haemolymph and viscera levels continue to fluctuate in a manner suggesting internal redistribution of mercury. After eight days of depuration, the viscera contains the most mercury and appears to be the only tissue to increase its proportionate share of the total tissue mercury. After fifteen weeks of depuration, residual mercury in whole clams is about 20% of initial concentrations. Acute decreases in salinity (15% S to 2% S) enhance Rangia's ability to depurate mercury.  相似文献   

3.
Fish living in contaminated environments may exhibit reduced growth which may be due, in part, to the impact of toxicants on prey capture ability. We studied the effects of methylmercury (meHg) and mercuric chloride (Hg) on feeding of Fundulus heteroclitus from Piles Creek, a polluted habitat in Linden, New Jersey. We found that exposure to 0·01 mg/liter of meHg for one week had less impact on the prey capture rate than exposure to a similar concentration of Hg. Exposure to 0·01 mg/liter for two weeks or to 0·02 mg/liter for one week, of either meHg or Hg, produced significant reductions in feeding rate, compared to controls. In general, the fish were affected to a greater degree by exposure to Hg than to meHg. This is the reverse of the usual order of toxicity, but is in keeping with previous observations on this population, in which we have noted that gametes and embryos had developed tolerance to meHg, but were more susceptible to Hg.  相似文献   

4.
The effects of zinc being added to sea water, to final concentrations of 0·1–20 ppm, have been studied on the heart rate, valve movements, mortality of Scrobicularia and on both isolated and in situ inhalant siphon preparations.The acute toxicity threshold for added zinc was determined to be about 10 ppm at 10°C. The median lethal times for 10 and 20 ppm zinc were 143·5 and 116·1 h respectively.The responses of Scrobicularia to zinc concentrations in sea water of between 0·1 and 10 ppm were tested by exposure for 6 h. Above 1 ppm, valve closure and bradycardia occur within 10–15 min. Below 5 ppm the valves subsequently opened and heart rate increased over the 6 h period, but in 10 ppm closure and pronounced bradycardia were maintained throughout.Addition of zinc, to final concentrations of 1, 5 and 10 ppm, had no effect on the isolated inhalant siphon in contrast to copper at 0·25 and 0·5 ppm which caused very marked siphonal contractions. However, when zinc (1–10 ppm) was added to an in situ inhalant siphon preparation, contractions occurred which were lost on removal of zinc from the bathing medium. Cutting the cruciform muscles medially resulted in the loss or delay of the response to zinc. This suggests the possibility of the cruciform muscle complex (muscle strands and associated sense organs) responding to zinc. This contrasts with the direct action of copper on the neuromuscular system of an isolated siphon.  相似文献   

5.
Two series of experiments were carried out to assess the long-term effects of the water soluble fractions (WSF) of fuel oils on the marine isopod, Sphaeroma quadridentatum. In one experiment, juvenile isopods (one month old) were exposed to sublethal concentrations (0·1% to 15% WSF) of a fuel oil (Baytown, Exxon) at room temperatures of 24 ± 1–6°C for nine months. These isopods survived to maturity and reproduced. However, growth rate was adversely affected at concentration ≥ 3% WSF and fecundity was depressed at concentrations ≥ 1% WSF. Survival of offspring in clean seawater was dependent to a large extent on the history of the previous generation. Offspring from groups exposed to ≥ 1% WSF experienced high mortality (> 70% within five weeks) even in clean seawater. This may imply that a population of Sphaeroma exposed to WSF as low as 0·2 ppm may eventually disappear, although animals can grow to maturity and reproduce at concentrations < 3 ppm.In another experiment, adult Sphaeroma (three months old) were subjected to the WSFs of four fuel oils (Baton Rouge, Baytown, Montana and New Jersey) for one month. Fecundity was depressed at a concentration > 15% WSF, regardless of the kind of fuel oil. On the other hand, the number of young produced bythe surviving females varied with the type of fuel oil for the same level of WSF. This may be attributed to different relative amounts of toxic components present in the WSF.  相似文献   

6.
After multiple autotomy (one chela and six walking legs) fiddler crabs (Uca pugilator) were exposed to zinc chloride, cadmium chloride, methylmercuric chloride or combinations of these metals. Zinc at 1, 3 and 5 ppm had a retarding effect on limb regeneration, which was amplified in water of reduced salinity. Methylmercury at 0·5 ppm and cadmium at 1·0 ppm have previously been found to retard regeneration. When crabs were exposed to meHg and Zn the effects were additive, both in sea water (30% salinity) and in water of reduced salinity (7–8%). However, when crabs were exposed to combinations of Zn and Cd the effects were antagonistic and the rate of regeneration was almost that of controls. In water of 7–8% salinity the Cd effect was greatly intensified and growth was almost totally prevented. The presence of Zn counteracted this and allowed growth and ecdysis to occur.  相似文献   

7.
The interaction of selenium with mercury was studied in the shrimp Palaemon elegans. The release of 203HgCl2 (5·0 μg Hg per gramme body weight) from shrimp pretreated with selenium (SeO2 doses of 1·97, 3·95 and 7·90 μg Se per gramme fresh weight) was significantly decreased compared with the control group to which only 5·0 μg Hg/g had been administered.In the presence of HgCl2 (5·0 μg Hg per gramme fresh weight) the release of 75Se also diminished significantly at the higher stable Se pretreated dose (7·90 μg Se/g) while, at a lower selenium concentration, the release was not statistically different. Analyses for stable Hg and Se confirmed the decrease in rate of selenium loss in the presence of mercury which had been demonstrated with radiotracers.A dose of 7·9 μg Se per gramme fresh weight injected 12 h before exposure of the shrimp to the various mercuric chloride solutions did not produce a significant difference in the 24 h LC50 compared with the group not pretreated with selenium. However, during exposure to mercury at 3·8 mg/litre, the median lethal time (LT50) for the shrimp pretreated for 4 days with sublethal selenium (6·9 and 10·5 mg Se/litre) was delayed (19·2 and 33·2 h) compared with the group which was not pretreated. The results are discussed in relation to the role of selenium in the acutely toxic effects of inorganic mercury.  相似文献   

8.
To identify some specific effects of organic contaminants on fisheries in an urbanized estuary we compared the reproductive success of starry flounder from San Francisco Bay with concentrations of tissue contaminants and hepatic mixed-function oxidase (MFO) activity. We found significantly lower (P < 0·05) sediment concentrations of total identified polynuclear aromatic hydrocarbons (PAHs) in the less urbanized San Pablo Bay (SP) area (Fig. 1) than in the more urbanized central bay (CB) stations (Table 1). For flounder in early gametogenesis (August and September) the SP fish (n = 20) had significantly lower (P < 0·01) liver concentrations of Aroclor 1260 (0·34 ± 0·14 μg/g) than those at the CB stations: Berkeley (BK, n = 20, 1·6 ± 1·6 μg/g); Oakland (OK, n = 16, 2·3 ± 2·8 μg/g); and Alameda (AL, n = 4, 2·2 ± 1 μg/g). A similar pattern existed for DDT concentrations: SP = 0·2 ± 0·16 μg/g; BK = 0·1 ± 0·34 μg/g; OK = 0·4 ± 0·53 μg/g; and AL = 0·4 ± 0·33 μg/g. Total PAHs in livers were as follows: SP = 0·14 μg/g; BK = 2·6 μg/g; OK = 1·4 μg/g; and AL = 14 μg/g. Although gonad index, liver index, and presence of fin rot are inversely related to aryl hydrocarbon hydroxylase (AHH) activity, healthy fish in a similar reproductive state have lower AHH activities in the SP area. For example, in August and September, 1984, mean AHH activities were as follows: SP = 203 ± 89, and CB = 355 ± 200 pmol 3-OH-B[a]P mg microsomal protein min. We found a log-linear relationship for AHH activity and its percent inhibition by 7,8-benzoflavone (10−4m) and only a few fish from SP showed enhanced AHH activity after addition of 7,8-benzoflavone. This suggests that most of the starry flounder in San Francisco Bay are induced.  相似文献   

9.
Concentrations of total recoverable inorganic tin (TRISn), monomethyltin (MeSn3+), dimethyltin (Me2Sn2+), trimethyltin (Me3Sn+) and (3-dimethylsulphonio)propionate (DMSP) were determined in leaves of Spartina alterniflora from three sites in the Great Bay estuary (NH) from 8 May to 15 September 1989. Total methyltin concentration increased from 8·9 ng g−1 (fresh weight) on 8 May to 472 ng g−1 on 23 May, decreased to 52 ng g−1 on 7 June and 16ng g−1 on 20 June, and remained low until the last sample on 18 September. Statistical calculations showed that methyltin concentrations varied significantly with sampling week, but not with site. DMSP concentrations showed very different behaviour. During the same sampling period DMSP concentrations varied only from 7·5 to 26 μmol g−1 (fresh weight). DMSP concentrations varied significantly for site, but not sampling week.  相似文献   

10.
Concentrations of tributyltin (TBT), dibutyltin (DBT), inorganic and total tin have been measured in water, sediments and deposit feeding clams, Scrobicularia plana, from 25 estuarine locations in England and Wales, in order to compare the bioavailability of different forms of the element. Abnormally high tin values in Scrobicularia from harbours and areas of high boating activity testify to the high bioaccumulation potential of organotins, notably TBT derived from anti-fouling paints, relative to inorganic tin.Infaunal deposit-feeding bivalves consistently contain higher concentrations of TBT than other benthic organisms indicating that sediments may be an important route for uptake. Significant correlations between sediment TBT values and residues in clams such as S. plana (r = 0·81, p < 0·001) support this hypothesis. Direct evidence of the bioaccumulation of sediment-bound TBT is provided from laboratory experiments in which Scrobicularia were exposed to TBT in water and in sediments, either separately or in combination. The results of these experiments clearly demonstrate a predominantly particulate component for TBT uptake in clams.The kinetics of TBT accumulation and elimination were studied in Scrobicularia exposed to sediments containing 0·3 and 1·0 μg/g TBT (as tin). Equilibrium concentrations in tissue are approached after 40 days.Sediments containing 10 μg/g TBT are acutely toxic to S. plana, although such concentrations are only likely to occur close to dockyards and large marinas. Preliminary laboratory and field observations suggest however, that clam populations could be affected at TBT concentrations in sediments of 0·3 μg/g and possibly lower.  相似文献   

11.
Pacific herring (Clupea harengus pallasi) larvae were exposed either directly to the water-soluble fraction (WSF) of crude oil or indirectly via oil-contaminated prey (OCP) (Artemia salina) for <- 28 days, to determine the relative effects of diet and water as routes of contamination. Larvae were affected rapidly by 0·9 ppm WSF, which caused high larval mortality, reduced swimming ability and rapid reductions in feeding rates. Larval length was significantly reduced by 0·7 ppm WSF in 7 days and by 0·3 ppm WSF within 14 days. The WSF exposure also caused similar reductions in larval weights. Highly contaminated prey (6 ppm prey exposure) caused significant mortality, but surviving larvae appeared robust. The OCP did not affect swimming, feeding, or growth. Exposure of larvae was not significantly extended by OCP, which rapidly depurated WSF in clean water (98% in 1 day). Therefore, OCP is probably not an important source of low molecular weight petroleum contamination to larval fish in the marine environment.  相似文献   

12.
-The detritus from the decomposition of dead plant materials of Spartina with great biomass provides abundant food for the heterotrophs in estuarine and nearshore coastal waters. It is for this reason that the decomposition rates of S. anglica and S. alterniflora near the estuary of obsolete Huanghe River were studied. The changes in nutritive components during decomposition are also studied.Results showed that the decomposition rate of dead leaves of S. anglica on ultra-low marsh was about 90% for the first year. In the eighth month, it was 80% and 76 % respectively for S. anglica and S. alterniflora on low marsh. Protein content of the two species of Spartina increased in the course of decomposition, with a maximum increment of 100% or more. The energy value also increased, whereas cellulose content decreased markedly. The percentage content of ash and lipid varied significantly with zonal differences.  相似文献   

13.
Axenic cultures of the microalgae species, Dunaliella tertiolecta and Phaeodactylum tricornutum were grown at arsenic (As) concentrations typically found in uncontaminated marine environments ( 2 µg L− 1) under different phosphorus concentrations. D. tertiolecta accumulated higher arsenic concentrations (mean: 13.7 ± 0.7 µg g− 1 dry mass) than P. tricornutum (mean: 1.9 ± 0.2 µg g−1 dry mass). Media phosphorus concentrations (0.6–3 mg/L) had little influence on microalgae growth rates or arsenic accumulation. Arsenic was present as lipid bound (29–38%; 4.2–9.5%), water-soluble (20–29%; 26–34%) and residue bound (41–45%; 57–69%) arsenic species in D. tertiolecta and P. tricornutum respectively. Hydrolysed lipids contained mostly glycerol arsenoribose (OH- ribose), dimethylarsinate (DMA) and inorganic arsenic (As(V)) moieties. Water-soluble species of microalgae were very different. D. tertiolecta contained inorganic arsenic (54–86%) with variable amounts of DMA (7.4–20%), arsenoriboses (5–25%) and traces of methylarsonate (MA) ( 1%). P. tricornutum contained mostly DMA (32–56%) and phosphate arsenoribose (PO4-ribose, 23–49%) and small amounts of OH-ribose (3.8–6.5%) and As(V) (9–16%). Both microalgae contained an unknown cationic arsenic species. The residue fractions of both microalgae contained predominately inorganic arsenic (99–100%). These results show that at natural seawater arsenic concentrations, both algae take up substantial amounts of inorganic arsenic that is complexed with structural elements or sequestered in vacuoles as stable complexes. A significant portion is also incorporated into lipids. Arsenic is metabolised to simple methylated species and arsenoriboses.  相似文献   

14.
Midsummer (1 August) population estimates of about 2 million O-group plaice (Pleuronectes platessa L.) were derived for sandy bays around the Firth of Forth in 1979–1980. This is an order of magnitude less than similar estimates made for the Clyde Sea Area in 1973–1974. Autumn population estimates of 0·4–1·0 million fish were comparable to estimates by the Ministry of Agriculture, Fisheries and Food for the area between the Scottish border and Flamborough Head (2·3 million for 1970 and 1973) which represented 4·8% (1973) to 5·3% (1970) of the total number of O-group fish on the English east coast.Largo Bay was the most important nursery area holding 25% of the total population. It is particularly well situated to receive newly metamorphosed plaice carried in water currents along the north side of the Forth from the spawning ground off Fife Ness. Plaice in the Forth are mainly distributed on fine to medium sandy beaches (186–480 μm), the mean number per haul in midsummer (D) being correlated with the median diameter (m.d. in μm) of the low water sediments by the equation: D=−45·7666+0·2327 m.d. (n=11,r=0·68,P<0·02 but>0·01).The shallow inshore water in sandy bays in the outer Firth was well mixed and more marine than estuarine (27·7–35·0‰). The correlation coefficient between fish density and water temperature was low, while that with salinity (S‰) was: D=6·1618+0·2238S (n=23,r=0·62,P<0·005).Regression analysis demonstrated that the relationship between the instantaneous mortality rate (Z) and the initial population density (Dp) was: Z×100=0·7480+0·0546dp (n=12,r=0·87,P<0·001).The mean mortality rate for the O-group plaice in the Forth nursery areas was 53% month−1.  相似文献   

15.
The efficacy of sodium hexametaphosphate, when added to a formalin-Rose Bengal fixative solution, in improving the efficiency of benthic sample sorting by encouraging the disaggregation of silt-clay sediment has been investigated. Samples of estuarine mud (Mdφ = 6·1) from Damhead Creek, River Medway, were used for the analysis. A significant increase (23%; p < 0·01) in sieving efficiency was found in samples treated with concentrations of 1·24g/litre or more of sodium hexametaphosphate; treatments with higher concentrations than 1·24g/litre did not improve on this performance significantly. The use of 1·24g/litre sodium hexametaphosphate in an initial fixative/preservative solution is recommended as an economic and practical means of improving the efficiency of benthic sample sorting, without deleterious effects on the animal specimens in the sample.  相似文献   

16.
Glutathione S-transferase activity was measured in the hepatic cytosol front Dissostichus mawsoni and Pagothenia borchgrevinki. Activity measures with 1-chloro-2,4-dinitrobenzene as substrate were 11·2 and 16·7 μmol/min/g tissue respectively. Little or no activity was detected when p-nitrobenzyl chloride or 3,4-dichloro-1-nitrobenzene were used as substrate. The hepatic glutathione S-transferases from D. mawsoni were partially purified using gel filtration and chromatofocusing. Three peaks of activity were resolved. The major isozyme (158-fold purification) eluting at pH7·1 appeared to be catalytically a homodimer. The isozyme was highly inhibited by triphenyltin chloride (IC50 = 0·1 μ) while inhibition constants for Cibicron Blue 3GA, bromosulphophalein and hematin were 1·1, 20 and 34 μ respectively.  相似文献   

17.
Suspended particle dynamics were investigated in the Ogeechee River Estuary during neap tide in July 1996. Samples were operationally separated into ‘ truly suspended ’ (settling velocity <0·006 cm s−1) and ‘ settleable ’ (settling velocity >0·006 cm s−1) fractions over the course of a tidal cycle to determine whether these two fractions were comprised of particles with differing biological and chemical characteristics. Total suspended sediment, organic carbon and nitrogen, chlorophyll a and phaeopigment concentrations were measured in each fraction, as well as rates of bacterial hydrolytic enzyme activity [β-1,4-glucosidase (βGase) and β-xylosidase (βXase)]. The majority of the suspended sediment (by weight) was in the truly suspended fraction; all measured parameters were largely associated with this fraction as well. When compared to the settleable material, the truly suspended material was significantly higher in % POC (5·7±0·6 vs. 3·9±1·8), % chlorophyll (0·07±0·02 vs. 0·03±0·01), % phaeopigment (0·030±0·006 vs. 0·018±0·012), and weight-specific maximal uptake rates (Vmaxper mg suspended sediment) of both enzymes (1·8±0·4 vs. 0·7± 0·2 nmol mg−1 h−1βGase and 1·1±0·3vs . 0·3±0·2 nmol mg−1 h−1βXase), providing clear evidence for a qualitative distinction between the two fractions. These results are interpreted to mean that the more organic-rich, biologically active material associated with the suspended fraction is likely to have a different fate in this Estuary, as ‘ truly suspended ’ sediments will be readily transported whereas ‘ settleable ’ sediments will settle and be resuspended with each tide. These types of qualitative differences should be incorporated into models of particle dynamics in estuaries.  相似文献   

18.
The effects on reproduction of single or chronic exposure to the direct-acting mutagen, radiation, were evaluated using Neanthes arenaceodentata as the model animal. In the first set of experiments, mated pairs were irradiated when oocytes were visible in the female and were given a single dose of either 0·5, 1·0, 2·0, 5·0, 10 or 50 Gy. In the second set of experiments, lifetime exposure was initiated upon the spawning of the P1 female and was terminated upon the spawning of the F1 female; the average total doses (0·55, 6·5 and 54 Cy) were delivered at a rate of 0·19, 2·1 or 17 mGyH−1. Our results on embryo abnormalities and mortalities indicated that lethal mutations were most likely induced in the germ cells and had an adverse effect on reproductive success by reducing the survival of early life stages. Except for those mated pairs exposed acutely to 10 or 50 Gy or exposed chronically to 17 mGyh−1, there was no evidence of gamete killing. However, evidence was obtained for mutagen-damage accumulation in developing gametes from continuous exposure to radiation.  相似文献   

19.
Crangon crangon (L.) (Crustacea: Decapoda) were subjected to three metals (Cd, Cu and Zn) at respective concentrations (0·35, 1·6 and 14·4mg/litre) that would induce 50% mortality in 5–6 days. Significantly different (P < 0·05) rates of mortality occurred in the three groups, although their ET50's were almost identical. No moultstage-dependent mortality was observed for Cd, but in Cu and Zn the post-moult stages (A and B) were more susceptible than either the intermoult (Ca and Cb) or premoult (D0–D4) stages.No significant (P> 0·05) sex- or size-dependent mortality was evident at the tested concentrations of these metals.Feeding and ecdysis were inhibited in all metals after day 3 and development from one moult-stage to the next was greatly reduced in Cd.Possible modes of toxicity have been discussed in terms of the physiological condition inferred from the moult-stage at the time of death.  相似文献   

20.
Hepatic levels of Cu, Zn and metallothionein (MT) in perch, caught in a Cu/Zn gradient from a brassworks, reflected the water concentration of Cu (1·0–9·4 ppb) and Zn (0·56–59 ppb). Significant correlations were found between hepatic Cu and MT levels (r = 0·72), and between Zn and MT levels (r = 0·69). There was an increase of the amount of Cu and Zn in the cytosolic fraction of the liver with increased hepatic levels of the metals. When liver samples. from perch caught at the most contaminated location, were run on a gel filtration column (Sephadex G-75) 78% of the cytosolic Cu and 24% of the Zn in the cytosol eluted together with MT.  相似文献   

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