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1.
Stable carbon and nitrogen isotope ratios have been determined on 41 strains ofArtemia sp. from different geographic regions around the world. The δ13C and δ15N values ranged between −13.7 to−25.0 per mil and −0.7 to 21.2 per mil respectively.Artemia δ13C values from coastal environments are consistent with a marine origin for the food sourceArtemia from inland salt lakes have a range of carbon isotope values suggesting C3, C4 and CAM based organic matter could form the base of theArtemia food chain. These data indicate thatArtemia having a wide range of carbon and nitrogen isotope values are available for tropho-dynamic research studies that quantify the effect of respired CO2 on tissue and CaCO3 shell13C/12C ratios. Such stable isotope variation also suggests that stable isotope fingerprinting remains a viable technique for identifying specificArtemia collection sites.  相似文献   

2.
San Jose lagoon is a hypersaline body of water located in Mexico in the Baja California Peninsula. The lagoon belongs to a system that lies between the fault ridge known as San Jose Creek. Because of its marine origin, it can be considered as thalassohaline, but its isolation from the ocean has brought about changes in its salt composition. It has an area of 13,500 m2, a mean depth of 80 cm and a total volume of 10,000 m3. It does not desiccate and can be considered as a permanent lagoon. Seasonal variations are small. TheArtemia population in San Jose produces cysts all year. To determine the physico-chemical conditions inducing permanent production of cysts, temperature, salinity, dissolved oxygen, and pH of the lagoon were monitored, as well as relative humidity and wind conditions in the region in different seasons of the year. From spring to summer, differences of 1 mg L–1 of O2, 1°C in water temperature, and 8 g L–1 in salinity were observed, and from summer to winter differences of 3.3 mg L–1, 6.5°C, and 14 g L–1, respectively. Despite small seasonal variations, the lagoon exhibits strong spatial and daily changes that are important for cyst production.  相似文献   

3.
Lake Hayward, Western Australia, is a pristine, monomictic coastal lake with a residual brine (depth=1.5 m) of 200 gL−1: stratification in winter (May to October) followed the input of ground water and rain. The epilimnion had temperature ranges of 10–30°C, and salinity from 65–110 g L−1. Maximum depth in winter was 2.4 m. The population structure of the brine shrimpArtemia parthenogenetica, the dominant metazoan grazer in Lake Hayward, was studied from September, 1989, to February, 1991. The L. Hayward population ofA. parthenogenetica is predominantly ovoviviparous with little recruitment into the adult population. Cyst production occurs in summer, and there was evidence for recruitment from cyst hatching in the winters of 1989 and 1990. Nauplii were continuously present throughout the study period. Nevertheless, recruitment through to adult stages as defined by the presence of gravid females occurred only twice: in October–December, 1989 and in December, 1990. Based on limited observations of long-term survival of laboratory cultures, we believe that the major mechanism controlling nauplius survival and recruitment ofArtemia in Lake Hayward is food quality and quantity.  相似文献   

4.
Variations of chlorophylla and Margalef's pigment diversity index were studied in two solar salt ponds at Aveiro (Esmolas and Tanoeiras). Portugal. In the Esmolas ponds, mean chlorophyll a values varied between 2.49 and 13.33 mg m−3 in the non-growing season, and between 5.43 and 40.55 mg m−3 in the growing season. In the Tanoeiras ponds, mean chlorophylla values were between 8.12 and 14.85 mg m−3 in the non-growing season, and 8.65 and 21.19 mg m−3 in the growing season. Fluctuations of Margalef's pigment diversity index were more irregular.  相似文献   

5.
The structure of the naturally occurringArtemia parthenogenetica population inhabiting the Mahalewaya Saltern in Sri Lanka was studied over an annual cycle, together with accompanying variations in the physico-chemical characteristics of its pond waters.Artemia was found only in the serially connected earthen ponds that serve as condenser pans in the saltern. Over the 12-month period of study, salinities in these ponds ranged from 81.5 to 199.6 ppt, temperatures from 28.2 to 33.6°C, pH from 7.9 to 9.1 and dissolved oxygen from, 1.1 to 9.0 mg L–1. The physico-chemical characters varied along a gradient across the three ponds, with salinity and temperature increasing with proximity of the condenser pans to the crystallizers of the saltern and dissolved, oxygen values showing the opposite trend. The ponds containingArtemia were shallow and had a maximum depth of 37 cm. Gross primary productivity ranged from negative values to 686.4 mg C m–2 day–1. Rainfall was distributed non-uniformly almost throughout the year, most of it being experienced from August to October, with a 254 mm maximum in October and lesser rainfall from December to May. Population numbers ranged from zero to 125 individuals L–1 and declined with increasing salinities and temperatures which related, spatilly, to increasing proximity of ponds to crystallizers and temporally, to the dry weather periods. Recruitment was best at low salinities and temperatures (104 to 114 ppt and 28.2 to 30.5°C) but also took place, although, at reduced intensities, up to 174 ppt salinity and 32.2°C temperature. Populations were mostly dominated by instar I stage nauplii with adult stages predominating only during periods of high salinity and temperature. Water management practices for salt production in the salterns included maintaining shallow depths that lead to high water temperatures and pond irrigation procedures that resulted in abrupt salinity changes. Such practices limited the size of theArtemia population as well as prevented its spread into the numerous ponds of the saltern.  相似文献   

6.
Zooplankton collections were made during 1985, 1986, 1988 and 1989 from 17 lakes in Saskatchewan and 3 in Alberta. Salinity ranged from 2.8 to 269 g L−1 (total filtrable residue). A total of 35 species was present in four taxa: Anostraca (3 species), Cladocera (11), Copepoda (7) and Rotifera (14). Species richness was greatest at salinities <7 g L−1 (15–16 species). Lakes with salinities between 7 and 100 g L−1 generally had 6–8 zooplankton species, while the most saline lakes (>100 g L−1) had 2–5 species. The largest concentrations of zooplankton occurred at <30 g L−1, but some species (Brachionus plicatilis, Hexarthra polyodonta, Artemia franciscana, Diaptomus connexus) were abundant at salinities >50 g L−1. Eurysaline species included the rotifersAsplanchna girodi (3–111 g L−1),Brachionus plicatilis (13–146) andKeratella quadrata (2.8–103).Artemia franciscana (33–269—but absent from Big Quill Lake, 49–82),Daphnia similis (3–104).D. connexus (9–82),Diacyclops thomasi (3–72), andCletocamptus albuquerquensis (17 to 126—but never abundant in the plankton). About half the species were restricted to hyposaline waters (3–20 g L−1), but some (Hexarthra fennica, Moina hutchinsoni, Hexarthra polyodonta) occurred only at intermediate salinities. The latter two species were also only present at high pH values (>9.2). There was a trend of decreasing species richness with increasing salinity. TWINSPAN classification of 94 lake samples (six parameters) based on zooplankton species abundances yielded a dendrogram with 14 ‘indicator’ species characteristic of seven lake groups related partly to a salinity gradient, but with other environmental factors such as water column depth, pH, Secchi disk transparency, water temperature and month sampled also influenced lake separation. *** DIRECT SUPPORT *** A02GG004 00002  相似文献   

7.
The apparent isotope enrichment factor εmacrophyte of submerged plants (εmacrophyte–DIC = δ13Cmacrophyte − δ13CDIC) is indicative of dissolved inorganic carbon (DIC) supply in neutral to alkaline waters and is related to variations in aquatic productivity (Papadimitriou et al. in Limnol Oceanogr 50:1084–1095, 2005). This paper aims to evaluate the usage of εmacrophyte inferred from isotopic analyses of submerged plant fossils in addition to analyses of lake carbonate as a palaeolimnological proxy for former HCO3 − concentrations. Stable carbon isotopic analysis of modern Potamogeton pectinatus leaves and its host water DIC from the Tibetan Plateau and Central Yakutia (Russia) yielded values between −23.3 and +0.4‰ and between +14.0 and +6.5‰, respectively. Values of ε Potamogeton–DIC (range −15.4 to +1.1‰) from these lakes are significantly correlated with host water HCO3 − concentration (range 78–2,200 mg/l) (r = −0.86; P < 0.001), thus allowing for the development of a transfer function. Palaeo-ε Potamogeton–ostracods values from Luanhaizi Lake on the NE Tibetan Plateau, as inferred from the stable carbon isotope measurement of fossil Potamogeton pectinatus seeds (range −24 to +2.8‰) and ostracods (range −7.8 to +7.5%) range between −14.8 and 1.6‰. Phases of assumed disequilibrium between δ13CDIC and δ13Costracods known to occur in charophyte swards (as indicated by the deposition of charophyte fossils) were excluded from the analysis of palaeo-ε. The application of the ε Potamogeton–DIC-HCO3 − transfer function yielded a median palaeo-HCO3 − -concentration of 290 mg/l. Variations in the dissolved organic carbon supply compare well with aquatic plant productivity changes and lake level variability as inferred from a multiproxy study of the same record including analyses of plant macrofossils, ostracods, carbonate and organic content.  相似文献   

8.
Zooplankton in saline lakes of the Southern Interior Plateau of British Columbia were collected on three occasions: mid-May and early August, 1990, and in late July, 1991. Salinities ranged from 2.6 to 45.8 g L−1. Of the 17 lakes examined, 13 were hyposaline (∼-20g L−1), four, mesosaline (20–50 g L−1) and none, hypersaline (>50 g L−1). pH ranged from 8.7 (Three Mile) to 10.7 (Goodenough), with values <9.6 in 10, and <9.0 in only three lakes. Lakes with high pHs had high bicarbonate-carbonate alkalinities. Thirty one species of zooplankton occurred: Protista (1), Rotifera (13), Anostraca (2), Cladocera (7), Ostracoda (1) and Copepoda (7). Sixteen species were restricted to hyposaline waters, with seven found only in salinities of ≤5 gL−1. Two species (Diaptomus connexus, Artemia franciscana) were restricted to mesosaline waters of salinity >35 g L−1. Eurysaline species includedBrachionus plicatilis, Hexarthra fennica, Limnocythere staplini, Artemia franciscana andDiaptomus connexus. Most species were abundant in 1+ lakes, but six species always had low populations.B. plicatilis, Hexarthra polyodonta, A. franciscana, Limnocythere staplini, Daphnia similis andD. connexus had densest populations in one or more of the three most saline lakes, viz. Long, Goodenough and Three Mile. Cladocerans other thanD. similis (Alona sp., Ceriodaphnia quadrangula, Daphnia pulex) were usually abundant in lakes of low salinity (<5 g L−1).Diatomus sicilis, present in all except the three most saline lakes, also occurred in abundance in ten of these lakes.Moina hutchinsoni andH. polyodonta were present in both hyposaline and mesosaline lakes, but, more importantly, tended to occur only at higher pH values (>pH 9.4) but not in all such lakes. These can be regarded as alkaline-saline species. Sorensen’s Coefficient of Community Similarity was used to compare communities in Alberta-Saskatchewan and British Columbia. It was low (0.37). A comparison restricted to just Cladocera gave a slightly higher value, 0.44, but a comparison of Copepoda gave a value of 71 per cent similarity between the regions. Multiple regression analyses using pH, TDS and K regressed on species richness for all samples accounted for only 41 per cent of the variance. However, when the analysis was seasonally restricted (May), and thus to a limited chemical range, 47 to 77.5 per cent of the variance was accounted for by these three variables.  相似文献   

9.
The hypermarine southern Coorong is threatened by proposals to drain relatively fresh surface water and groundwater from adjacent agricultural areas into the Coorong. These influent waters carry moderate loads of heavy metals. Acute toxicity of heavy metals toDiacypris compacta, an abundant ostracod in the Coorong, was measured in the laboratory at 18°C in a static system using Coorong water (pH 7.8 salinity 50 ppt). At 4 days (96 h) the mean values of LC50 for copper, zinc, lead and, cadmium respectively were 0.8, 2.1, 3.1 and 4.3 mg L–1, and at 8 days the respective mean LC50 s were 0.4, 0.7, 2.2 and 1.1 mgL–1. The effect of two or three metals on mortality was additive in some cases and synergistic in other cases, but generally less than additive. However, in all cases mortality was greater in the presence of two or three metals than in the presence of a single metal. According to ANZECC (1992) guidelines, maximum acceptable concentrations of heavy metals should be no higher than 0.01 x the lowest LC50 value. Using the lowest LC50 values forDiacypris compacta obtained at 8 days, maximum acceptable concentrations in the Coorong would be 4, 5, 9 and 22 gL–1 for copper, zinc, cadmium and lead respectively, the values for zinc and copper failling below those recommended by ANZECC (1992) for marine waters. Reported concentrations of copper and zinc in surface water and groundwater in areas adjacent to the Coorong sometimes exceed these values, hence drainage of these waters into the Coorong represents a significant hazard to the Coorong biota.  相似文献   

10.
Groundwater is the major source of drinking water in Nahavand city. However, the groundwater quality at the agricultural areas has been deteriorating in recent years. Ground water quality monitoring is a tool which provides important information for water management and sustainable development of the water resources in Nahavand. Hydrochemical investigations were carried out in an agricultural area in Nahavand, western Iran, to assess chemical composition of groundwater. In this study, 64 representative groundwater samples were collected from different irrigation wells and analyzed for pH, electrical conductivity, major ions, and nitrate. The results of the chemical analysis of the groundwater showed that concentrations of ions vary widely and the most prevalent water type is Ca–Mg–HCO3, followed by other water types: Ca–HCO3, Ca–Na–HCO3, and Na–Cl, which is in relation with their interactions with the geological formations of the basin, dissolution of feldspars and chloride and bicarbonate minerals, and anthropogenic activities. Thirty-seven percent of the water samples showed nitrate (NO3 −) concentrations above the human affected value (13 mg L−1). The phosphorous (P) concentration in groundwater was between 0.11 and 0.90 mg L−1, with an average value of 0.30 mg L−1, with all of the samples over 0.05 mg L−1. The most dominant class C2-S1 (76.5%) was found in the studied area, indicating that sodicity is very low and salinity is medium, and that these waters are suitable for irrigation in almost all soils. Agronomic practices, such as cultivation, cropping, and irrigation water management may decrease the average NO3 − concentration in water draining from the soil zone.  相似文献   

11.
During May and June 1991, the biological and ecological features of sixteen aquatic localities in Zhanjiakou region, Hebei, northern China, were investigated. Their salinity ranged from 0.98 to 175.2 g L−1. Fish and benthic macroinvertebrates were depauperate. 116 samples were collected. 91 species (or genera) of phytoplankton and 94 species of zooplankton were recorded. In most of the saline lakes, Cl− and Na+ were dominant, and alkalinity and pH values were higher. For phytoplankton, major species wereChroococcus minutus, Oscillatoria tenuis [blue-green algae];Chlamydomonas spp.,Chlorococcus spp.,Chlorella vulgaris, Crucigenia quadrata, Dunaliella salina, Kirchneriella contorta, Oocystis lacustris, Scenedesmus acuminatus [green algae]; andCyclotella meneghiniana, Navicula spp.,Nitzschia spp. [diatoms]. Other phytoplankton taxa present included:Cryptomonas erosa, Chroomonas acuta, Chromulina pascheri, Euglena viridis. For zooplankton, major species included:Vorticella campanula, Epistylis breviramosa, Euplotes terricola [protozoa];Brachionus plicatilis, Hexarthra fennica, Keratella quadrata, Notholca acuminata quadrata [Rotifera];Daphnia magna, D.longispina [‘Cladocera’];Artemia spp. [Anostraca];Arctodiaptomus rectispinosus andCyclops vicinus [Copepoda]. The species composition, distribution, salinity tolerance of planktonic forms in inland saline waters are discussed and their relationship with certain environmental factors.  相似文献   

12.
This study demonstrates use of compound-specific radiocarbon analysis (CSRA) for dating Holocene lacustrine sediments from carbonate-hosted Ordy Pond, O‘ahu, Hawai‘i. Long-chain odd-numbered normal alkanes (n-alkanes), biomarkers characteristic of terrestrial higher plants, were ubiquitous in Ordy Pond sediments. The δ13C of individual n-alkanes ranged from −29.9 to −25.5‰, within the expected range for n-alkanes synthesized by land plants using the C3 or C4 carbon fixation pathway. The 14C ages of n-alkanes determined by CSRA showed remarkably good agreement with 14C dates of rare plant macrofossils obtained from nearby sedimentary horizons. In general, CSRA of n-alkanes successfully refined the age-control of the sediments. The sum of n-alkanes in each sample produced 70–170 μg of carbon (C), however, greater age errors were confirmed for samples containing less than 80 μg of C. The 14C age of n-alkanes from one particular sedimentary horizon was 4,155 years older than the value expected from the refined age-control, resulting in an apparent and arguable age discrepancy. Several lines of evidence suggest that this particular sample was contaminated by introduction of 14C-free C during preparative capillary gas chromatography. This study simultaneously highlighted the promising potential of CSRA for paleo-applications and the risks of contamination associated with micro-scale 14C measurement of individual organic compounds.  相似文献   

13.
The main limnological features of Lake Issyk-kul are described. The lake is a large (6,236 km2), deep (zm, 668 m), closed lake in eastern Kirgizia. It lies at ∼1,607 m above sea level, but water-levels have been dropping since the last century. It is slightly saline (salinity, ∼6g L−1), with Na+, Mg2+, Cl− and SO 4 2− the dominant ions. Nutrient levels are low and the lake is considered ultra-oligotrophic. Characeae dominate the macrophytes. About 300 and 117 taxa of, respectively, phytoplankton and zooplankton have been identified, withArctodiaptomus salinus the most numerous in the zooplankton. Chironomids dominate the benthos. Several endemic taxa of fish occur, of whichLeuciscus bergi was dominant until the 1970s. The fish fauna has been supplemented by many introduced species. Three mysids were introduced in 1965–8 and are now a significant part of the ecosystem. The present annual fish catch permitted is 320 t. The most important value of the lake is as a recreational resource. To promote and sustain this value requires careful, ongoing management. The most significant threats to the lake are local pollution, visitor pressure, and declining water-levels.  相似文献   

14.
We quantified differences in oxygen isotope fractionation among three biostratigraphically important subfossil ostracod species (Metacypris cordata, Pseudocandona rostrata and Candonopsis kingsleii) from an early Holocene freshwater tufa layer in northern Estonia. Estimated mean δ18O values are −10.05‰ for M. cordata, −9.34‰ for C. kingsleii and −8.75‰ for P. rostrata. All three species exhibit positive offset from the weighted mean annual δ18O of contemporary precipitation (−10.7‰ in δ18OV-PDB) and from the mean δ18O value of authigenic tufa carbonate (−10.64‰) in the ostracod-bearing layer. Assuming that the known oxygen isotope fractionation in P. rostrata (+2.5‰) and M. cordata (+1.5‰) has remained constant over time, the theoretical δ18OV-SMOW of the early Holocene lake water was calculated to have been between −11.52 and −11.92‰, slightly less negative than the local Ordovician groundwater (−11.7 to −12.2‰). δ18O values of the tufa carbonate differ by +0.6 to +1.0‰ from the calculated theoretical isotope composition (δ18OV-PDB) of lake water, indicating that the tufa also did not precipitate in isotopic equilibrium with ambient waters. Results show that the greater the δ18O offset from the calculated, theoretical isotope composition of lake water for an ostracod species, the lower is its preferred mean July temperature. Both our data and earlier published results on δ18O values in Holocene lacustrine carbonates and ostracods from north-eastern Europe, display pronounced decreases in δ18O with an increase in latitude of the study site. This suggests that temperature-dependent, and therefore latitude-dependent isotopic composition of meteoric waters controlled the δ18O values in lacustrine tufa and ostracods throughout the Holocene.  相似文献   

15.
Macroinvertebrate communities were studied in two saline tributaries of the Hopkins River, western Victoria. Monthly sampling from May to August, 1990, showed the mean salinity of Bushy Creek was 5 g L−1 while in Back Creek mean salinity varied from 9 to 20 g L−1 between sites. Macroinvertebrate species richness and relative abundance were measured on each sampling occasion. Cluster analysis showed that community composition varied between creeks and between sites within a creek. Bushy Creek sites were more similar to each other than to Back Creek sites. A negative relationship was found between site salinity and species richness over the range 4 to 26 g L−1, although there was a wide range of salinity (up to 12 g L−1) over which the relationship of species richness to salinity was not clear cut. The effects of salinity on species richness in stream communities appear to be similar to that described elsewhere in salt lakes.  相似文献   

16.
We developed an inference model to infer dissolved organic carbon (DOC) in lakewater from lake sediments using visible-near-infrared spectroscopy (VNIRS). The inference model used surface sediment samples collected from 160 Arctic Canada lakes, covering broad latitudinal (60–83°N), longitudinal (71–138°W) and environmental gradients, with a DOC range of 0.6–39.6 mg L−1. The model was applied to Holocene lake sediment cores from Sweden and Canada and our inferences are compared to results from previous multiproxy paleolimnological investigations at these two sites. The inferred Swedish and Canadian DOC profiles are compared, respectively, to inferences from a Swedish-based VNIRS-total organic carbon (TOC) model and a Canadian-based diatom-inferred (Di-DOC) model from the same sediment records. The 5-component Partial Least Squares (PLS) model yields a cross-validated (CV) RCV2 R_{CV}^{2}  = 0.61 and a root mean squared error of prediction (RMSEP CV ) = 4.4 mg L−1 (11% of DOC gradient). The trends inferred for the two lakes were remarkably similar to the VNIRS-TOC and the Di-DOC inferred profiles and consistent with the other paleolimnological proxies, although absolute values differed. Differences in the calibration set gradients and lack of analogous VNIRS signatures in the modern datasets may explain this discrepancy. Our results corroborate previous geographically independent studies on the potential of using VNIRS to reconstruct past trends in lakewater DOC concentrations rapidly.  相似文献   

17.
Biomonitoring past salinity changes in an athalassic subarctic lake   总被引：1，自引：0，他引：1  
A short sediment core was taken from a small saline lake located on an intermontane plateau in the central Yukon Territory, Canada. In July 1990, chemical analyses indicated that, although the lake was shallow (Zmax=1.1 m), it was also chemically stratified, with hyposaline (9.9 to 10.0 g L−1) surface waters and slightly mesosaline (22.0 g L−1) deeper waters. The surface water was dominated by Na+ and HCO 3 − . To our knowledge, this is the northernmost athalassic saline lake yet recorded. Quantification of algal (diatom, chrysophyte, and pigment) and invertebrate (chironomid, ceratopogonid, andChaoborus) fossils at four stratigraphic levels indicated that the lake sediments preserved numerous biological indicators that could be used to infer recent lake development. Many of the taxa are found in other athalassic salt lakes. The most striking stratigraphic change was a remarkable drop in the species richness of diatoms and invertebrates in the recent sediments, which parallels the elimination of species characteristic of less saline conditions. Halophilous taxa dominate the most recent sediments, indicating the development of more saline conditions. At the same time, a significant shift in chrysophyte cyst composition was observed. Fossil carotenoids and chlorophylls indicated a decrease in total algal abundance in recent sediments, as green and blue-green algae replaced diatoms and chrysophytes. Together, these paleolimnological data suggest a recent shift to drier conditions or increased evaporation in the central Yukon Territory.  相似文献   

18.
We present electron microscopic observations on fragments of encysted embryos of the crustacean,Artemia sp., recovered from a 27,000 year-old core section taken under the Great Salt Lake (GSL), Utah. The significance of these results comes from a consideration of the geological history of the GSL and the extraordinary properties and abundance ofArtemia cysts at this site. We make the case that this unique system, and probably other salt lakes as well, provides novel opportunities for the study of “ancient DNA” and related matters. The analysis provides the major focus of this paper, which we hope will stimulate interest in this aspect of salt lake research.  相似文献   

19.
Chrysophyte cysts and scales and sponge spicules were enumerated, along with diatoms, from the surface sediments of 102 western Canadian lakes. The salinities of these lakes ranged from 0.042 to 369 g L−1 in late summer. Sponge spicules and chrysophyte cysts and scales were more common in fresher waters, although chrysophyte cysts were also present in subsaline and hyposaline waters at lower relative percentages. These siliceous microfossils can easily be distinguished and counted along with diatom valves, with little extra effort. It is likely that using these additional indicators will strengthen paleolimnological inferences of past lakewater salinity.  相似文献   

20.
A sediment core from Lake Koucha (eastern Tibetan Plateau) was investigated using organic biomarkers and their stable carbon isotope signatures. The correlation between TOC content, total amount of aquatic macrophyte-derived n-alkanes (e.g. nC23) and δ13C values of TOC and nC23 indicates that Lake Koucha was macrophyte-dominated before 8 cal ka BP. Shortly after the lake turned from a saline to a freshwater system at 7.2 cal ka BP, a variety of algal and bacterial markers such as hopanoids and isoprenoids emerged, of which phytane, pentamethylicosene (PMI), moretene and diploptene are particularly abundant. Phytane and PMI show different isotopic signals (≈−18 and ≈−28‰, respectively), which indicates that they originated from different sources. Phytane may have been derived from cyanobacteria, while methanogenic archaea may be the source of PMI. The isotopic depletion of diploptene and moretene (≈−60‰) indicates the presence of methanotrophs. After 6.1 cal ka BP, the saturated C20 highly branched isoprenoid (HBI) became the dominant constituent of the aliphatic hydrocarbon fraction. Such dominance has rarely been reported in lacustrine environments, and indicates a strong presence of algae (most likely diatoms) or cyanobacteria. At 4.7 cal ka BP, the appearance of an unsaturated C25 HBI, which is a specific biomarker for diatoms, was noted. Furthermore, the level of nC17-alkane was observed to increase in abundance in the uppermost two samples. These results suggest that the lake was phytoplankton-dominated during the last 6.1 ka. Relatively low biomarker concentrations and δ13C values at 6.0, 3.1 and 1.8 cal ka BP indicate the occurrence of cool periods, which is in agreement with inferences from other locations on the Tibetan Plateau. The δ13C values of nC23 range from −23.5 to −12.6‰, with high values at the peak of macrophyte abundance at ca. 11 cal ka BP and at the phytoplankton maximum between ca 6.1 and 2.8 cal ka BP. Thus, aquatic macrophyte-derived mid-chain n-alkanes have been found to be excellent indicators of carbon-limiting conditions, which lead to the assimilation of isotopically-enriched carbon species. The limitation of carbon sources could be a localized phenomenon occurring in dense plant stands (as in the older section of the core), or it may be induced by high primary productivity (as in the younger section). Since the δ13C value of the inorganic carbon source may vary, the offset between the δ13C values of nC23 and TIC could serve as a more precise proxy for carbon-limiting conditions in lacustrine environments, which could in turn be interpreted with respect to lacustrine paleo-productivity.  相似文献   

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