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1.
We performed a karyotype analysis on four species from Phylum Nemertea using regenerating somatic cells. Two palaeonemertean species, Cephalothrix hongkongiensis and Cephalothrix sp., had the same chromosome number (2n=28), but different karyotypes (16 m+10 sm+2 st, NF=54 and 22 m+4 sm+2 st, NF=54, respectively). The karyotypes of the two heteronemerteans, Notospermus geniculatus and Ramphogordius sanguineus were 2n=38, 30 m+6 sm+2 sm/st (NF=76/74) and 2n=56, 48 m+4 sm+4 sm/st (NF=112/108), respectively. 相似文献
2.
To establish a micropropagation system of three Laurencia complex species (Laurencia okamurai, Laurencia tristicha, and Chondrophycus undulatus) by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal
fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under
a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity
levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with
0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments
in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the
basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary
and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20°C and 35 (salinity); for C. undulatus, 4 500 lx, 20°C and 30; and for L. tristicha, 4 500 lx, 25°C and 30. 相似文献
3.
To establish a micropropagation system of three Laurencia complex species (Laurencia okamurai, Laurencia tristicha, and Chondrophycus undulatus) by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20°C and 35 (salinity); for C. undulatus, 4 500 lx, 20°C and 30; and for L. tristicha, 4 500 lx, 25°C and 30. 相似文献
4.
A fragment of a large sub-unit ribosomal DNA (LrDNA) of 12 strains of Prorocentrum species was amplified by polymerase chain reaction (PCR). The PCR products were digested by 3 restriction endonucleases (Cfo Ⅰ, Hae Ⅲ, and RSA Ⅰ) and then resolved in agarose gels. Results show that different species had different RFLP patterns, except for P arcuatum (ME 131), which had the same pattern to P. micans (ME 160 and 04).The same fragment of 19 strains of the genus was also amplified and subjected to denaturing gradient gel electrophoresis (DGGE). 11 different patterns were resolved. Different cultures of a same species had the same pattern. The results of RFLP and DGGE analyses showed that eight newly isolated epibenthic Prorocentrum species were different from each other, and also from other cultured ones examined in this study. P arcuatum(MEI32) could not be differentiated from P. micans (MEI60 and 04), it was probably mis-identified, since they are quite different morphologically. P. redfieldii (MEI38) could also not be distinguished form P. triestinium(MEI32), it should be regarded as a synonym ofP. triestinium. Unexpectedly, a restriction site was found in P.micans, compared with previous sequence data. 相似文献
5.
Based on material deposited in the Marine Biological Museum of the Chinese Academy of Sciences(Qingdao),a new species,Lumbrineris sinensis sp.nov.,as well as Augeneria albidentata(Ehlers,1908),which is recorded for the first time from coastal water of China,are reported in the present paper.The specimens examined were collected during 1958 to 1960 from the Yellow Sea,East China Sea and South China Sea at depths of 4 to 182 m. 相似文献
6.
A morphological study of a marine red alga from China, previously identified by various authors as “Solieria mollis (Harv.) Kylin”, has been shown to be an undescribed species ofSolieria. The present new speciesSolieria tenuis is characterized by the slender branches, the unbranched and nonseptate connecting filament and the discoid holdfast surrounded
by prop branches resembling proproots of land plants.
Contribution No. 989 from the Institute of Oceanology, Academia Sinica. 相似文献
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8.
Degradation of slopes due to shallow landslide and the subsequent erosional processes are a big challenge on the application of soil bioengineering techniques; that is the use of plants as main structural components of a slope protection and conservation system. An optimal application of soil bioengineering techniques should include not only the technical factor of plants as structural components but also the ecology of species and the plant adaptations to disturbances, which is crucial if a longterm successful slope restoration system is intended. Ferns are a dominant understory vegetation species in the forest of Japan, but its characteristics and influences on the recovery of shallow landslide scars have not been fully studied yet. This study aims to find out the ecological characteristics of fern species through the calculation of ecological indicators and the quantification of the morphological features of specimens growing on disturbed and non-disturbed forest slopes in Japan. Gleichenia japonica was found as the vegetation species with biggest ecological indicators on both slopes. The analysis of morphological characteristics of the specimens growing on both sites showed that the development of the specimens is focused in below-ground characteristics. The pull-out force of Gleichenia japonica root system as an indicator of ecological adaptation to a constraint environment and morphological characteristics quality is influenced by height and root length according to the principal component analysis. The eco-morphological characteristics of species can be used as an indicator of an optimal element in soil bioengineering establishment for slope conservation proposes. The long and fibrous root system could be placed on forest roads, steep or small slopes where space limitation is an issue for the establishment of bigger species and if the slope conditions allow it, it can control soil losses due to rainfall and provide stability. 相似文献
9.
《中国海洋湖沼学报》2021,(2)
One new species and one new record species of Sphaerolaimus are described.Sphaerolaimus longispiculatus sp.nov.is characterized by body length 2 213-2 470 μm,amphideal fovea 12%-16%corre sponding body diameters in males and 9%-10% corre sponding body diameters in females,long slender spicules 2.8-3.8 cloacal body diameters,eight small papilliform precloacal supplements,gubernaculum without apophysis,and tail conico-cylindrical,2.5-3.7 cloacal/anal body diameters long.Sphaerolaimus gracilis is recorded from the first time in the mangroves wetlands,it can be distinguished from the original desceiption by longer spicules(66-71 μm vs 56 μm) and wider body(a=20.1-21.4 vs 25). 相似文献
10.
Journal of Oceanology and Limnology - Large-scale Ulva-caused green tides has posed various environmental and ecological problems as well as economic consequences. It is important to identify them... 相似文献
11.
Journal of Oceanology and Limnology - Three species of Siphonalia A. Adams, 1863 collected from the East and South China Seas are described and illustrated. Species were identified during... 相似文献
12.
Ripu M. KUNWAR Laxmi MAHAT Lila N. SHARMA Keshab P. SHRESTHA Hiroo KOMINEE Rainer W. BUSSMANN 《山地科学学报》2012,9(5):589-600
Underutilized plant species help to alleviate common food insufficiencies by providing alternative food supply. They also complement primary health care, furnishing raw materials where the cultivation of staple cereal crops is least feasible and health care is pursued indigenously. Research and promotion of extraction, utilization, and conservation of underutilized species lead to exploration of new staple crops and motivate people to consume in a sustainable manner. The present study describes the current status, uses, and management of underutilized plant species in Far West Nepal. The relative importance of 49 underutilized plant species was computed employing a Relative Importance (RI) technique. The use-values assigned to the species fall into six use-categories: beverage, fodder, food & edible, medicinal, vegetable and veterinary. A total of 22 species appeared in multiple use-categories, while the rest were characterized by a single use-category. Based on relative importance and frequency, Ficus semicordata, Debregesia longifolia, Girardinea diversifolia, Hydrocotyle nepalensis, Garuga pinnata, Aloe vera and Pyrus pashia offer the most potential for future. Underutilized plants proved important to folk medicine and food. These species persist because they remain useful to local people as means of subsistence, production, and primary health care. The findings are important so far as they point up the role of underutilized plants in national food security policy and health care, spelling out their potentialities and cross cutting relationships. 相似文献
13.
This paper reports results of study on the contents of proteins, amino acids, polysaccharose and uronic acids in 30 species of macroalgae from Shicao, Heishijiao, Shimiao, and Xiaofujiazhuang in the vicinity of Dalian City, N.E.China. The results showed that the protein contents of the 30 algae from highest (112.55μg/ml) to the lowest (0.24 p.g/ml) was in the descending order of Dictyopteris ndalata, Gelidium vagum,Gymnogongrus japonican, Ectocarpus confervoides, Tinocladia crassa, Sargassum thunberii. In general, the protein content in red algae was higher than that in brown algae. The content of free ami no acids showed nosignificent differences from 7.44μg/ml-4.96μg/ml in all these algae, in the descending order of Gymnogongrusrus japonican, Sargassum confusum, Undoria pinnatifida, Laminaria japonica and Ectocarpus confervoides. The content of polysaccharose varied from 168.2 gg/ml-22.15 p.g/ml in the descending order of Symphyocladiaia tiuscula, Scytosiphon lomentarius, Desmarestia viridis., Tinocladia crassa, Gracilaria asiatica and Porphyra yezoensis. The content of uronic acids is from 196.24μg/ml-20.77μg/ml in the descending order of Ulva lactuca,Symphyoclaldia latiuscula, Scytosiphon lomentarius, Ceramimum kondoi, Gracilaria vemucosa and Porphyra yezoensis. The fatty acids in 30 species of algae belong to Rhodophyta, Chlorophyta and Phaeophyta. Most phaeophytes have many (4-12) types of fatty acids. 相似文献
14.
Yingchun Lv Rong Zheng Tao Zuo Yuming Wang Zhaojie Li Yong Xue Changhu Xue Qingjuan Tang 《中国海洋大学学报(英文版)》2014,13(5):825-829
Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible. 相似文献
15.
梁华芳 《广东海洋大学学报》2001,21(2)
本文对我国养殖的 9种对虾主要形态特征作了简要的描述 ,并根据它们的不同特征把这 9种对虾分成 4个组 :即中国对虾组、斑节对虾组、日本对虾组和凡纳对虾组。对各组间和组内不同种类对虾的形态特征作了比较 ,并根据它们不同的形态特征编出检索表 ,以便于生产上应用。 相似文献
16.
Toxic effects of zinc on four species of freshwater fish 总被引：1，自引：0，他引：1
The toxic effects of Zn2+ on Silver Carp (Hypophthalmichthyps molitrix C. et V.), Big Head Carp (Aristrichthys nobilis Richardson), Grass Carp (Ctenopharyngodon ilellus C. et V.), and Blunt Snout Bream (Megalobrama aimbly-cephala Yih) are studied. The test results are: (1) There are linear correlations between 24h LC50 and 48h LC50 of Zn2+ for Silver Carp fingerling and temperature. 24h LC50, 48h LC50 and 96h LC50 of Zn2+ for fry of the four species are also determined; (2) There are logarithmic correlations between the growth rates of the fry
and the concentrations of Zn2+ and between expansion of fish egg membranes after absorbing water and concentrations of Zn2+; (3) The tolerance of fry of the four species to Zn2+ is in the following order: Grass Carp>Silver Carp>Blunt Snout Bream>Big Head Carp; (4) The safe concentrations of Zn2+ are: Big Head Carp: 0.008 mg/L, Grass Carp: 0.046 mg/L, Blunt Snout Bream: 0.010 mg/L, Silver Carp: 0.012 mg/L, Silver Carp
fingerling: 0.09 mg/L. 相似文献
17.
To understand the impacts of reforestation on woody species composition, species diversity and community structure, seven plantation forests in dry-hot valley of the Jinsha River in Southwest China were investigated, with adjacent wastelands, natural shrub grassland and a natural forest as references. Species importance value, species richness, species heterogeneity and Sorenson similarity index between plantations and the natural forest were analyzed. Results indicated that compared to wastelands and natural shrub grassland, reforestation improved species diversity and community structure, and more forest woody species found suitable habitats in plantations. Species diversity in understory of plantations and Sorenson similarity index were significantly negatively correlated with stem density in mature plantations (26-31 years old). Higher species diversity and Sorenson similarity index existed in mature sparse plantations due to lower stem density and more tree species planted initially. In contrast, reference natural forest, with species heterogeneity of 2.28 for shrub layer, showed the highest species diversity. It would take a long time for species composition and diversity to recover through reforestation in a dry-hot valley. Therefore, it was essential to protect remnant natural forests strictly and reforest with suitable management such as lower stem density and increasing genetic diversity of trees planted. 相似文献
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19.
DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding (cytochrome c oxidase subunit I (COI)), the mtDNA control region and cytochrome b. The results reveal that: 1) intraspecific variation in the DNA barcode ... 相似文献
20.
Five galatheid squat lobster species belonging to four genera of two galatheid families are reported for the first time from Chinese waters, namely Lauriea simulata Macpherson and Robainas- Barcia, 2013, Phylladiorhynchus ikedai （Miyake and Baba, 1965）, Phylladiorhynchus integrirostris （Dana, 1852）, Babamunida sp., and Raymunida elegantissima （de Man, 1902）. The genera Lauriea Baba, 1971 and Babamunida Cabezas, Macpherson, and Machordom, 2008 have not previously been recorded from Chinese waters. 相似文献