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1.
In order to determine the role of alginate-derived oligosaccharides （ADO） in drought stress resistance of tomato （Lycopersicon esculentum Miller） seedlings, the leaves were exposed to different concentrations of ADO （0.05%, 0.10%, 0.20%, 0.30% and 0.50%） after drought stress was simulated by exposing the roots to 0.6molL^-1 PEG-6000 solution for 6h. Changes in biomass, electrolyte leakage and malondialdehyde （MDA）, free proline, total soluble sugars （TSS） and abscisic acid （ABA）, the enzyme activities of catalase （CAT）, superoxide dismutase （SOD）, peroxidase （POD） and phenylalanine ammonia-lyase （PAL） were measured to investigate the effects of ADO treatment. The results showed that the treatment with an ADO concentration of 0.20% exhibited the highest performance of drought stress resistance in the tomato seedlings by decreasing the electrolyte leakage and the concentration of MDA, increasing the contents of free proline, TSS and ABA, and increasing the activities of CAT, SOD, POD and PAL after treatment with ADO. It is suggested that changes in electrolyte leakage, MDA, osmotic solutes, ABA, anti-oxidative enzyme and PAL activities were responsible for the increased drought stress resistance in tomato seedlings. To our best knowledge, this is the first report of the effect of ADO treatment on enhancing the drought stress resistance of tomato seedlings.  相似文献   

2.
For sustainable water resource management,it is important to determine the relationship between streamfl ow and other variables that infl uence availability of water resources.However,many catchments do not have suffi cient data to allow for a more detailed study of these relationships.We faced a similar challenge in the Chengcun Basin(limited historical data: from 1986–1999); and therefore we used a new approach to overcome this.We found that,using nonparametric trend methods in conjunction with the climate elasticity analyses and the 2D visualization of hydrologic data,it is possible to assess the relationships between streamfl ow and other hydro-climatic variables.In the past,streamfl ow trends in the basin were more correlated with precipitation than with potential evapotranspiration(PET).In addition,there is a gradual shift in the hydrological regime of the catchment,which may affect the occurrence of available water resources and activities that depend on them.In addition,based on our climate sensitivity analyses,the streamfl ow is dependent and more sensitive to variations in precipitation than to PET(δQ=0.79δP+0.42δE; precipitation elasticity,ε P=1.32; PET elasticity,ε E=-2.10).Therefore pending more detailed studies,the use of our approach will provide a rapid means to assess the variation of water resources(streamfl ow)in a watershed.In the future,we hope to carry out related research in other watersheds and also perform a more detailed studies to improve upon the results of this study.  相似文献   

3.
The dynamic microcosms were used to evaluate the effect of oil spills on microbial ecological system in marine sedi- ment and the enhancement of nutrient on the oil removal. The function and structure of microbial community caused by the oil pollu-tion and phosphate dosage were simultaneously monitored by dehydrogenase activity assay and PCR-denaturing gradient gel elec- trophoresis （DGGE） techniques. The results indicated that the amount of total bacteria in all dynamic microcosms declined rapidly with incubation time. The number of petroleum-degrading bacteria and the activity of sediment dehydrogenase were gradually en-hanced by petroleum in the oil-treated microcosms, while they both showed no obvious response to phosphate dosage. In comparison, phosphate spiked heterotrophic bacteria and they showed a significant increase in amount. DGGE profiles indicated that petroleum dosage greatly changed community structure, and the bacteria belonged to class Deltaproteobacteria, and phyla Bacteroidetes and Chlorobi were enriched. This study demonstrated that petroleum input greatly impacted the microbial community structure and con- sequently the marine sediment petroleum-degrading activity was enhanced. Phosphate dosage would multiply heterotrophic bacteria but not significantly enhance the petroleum degradation.  相似文献   

4.
The generality to anisotropy of the earth media can be proved by a lot of research and observation, which is shown by the elastic parameters of the seismic wave changes with the direction of propagation. Actually, computational efficiency is very low, when simulating on the elastic anisotropie media, for the complicated and multi-parameters computation, which becomes a disadvantage to the succeeding migration and imaging pro-cedure. Using acoustic approximation in the VT[ media is an advisable simplification for the elastic wave simu-lation in that setting S-velocity into zero can greatly reduce the computational amount as well as get the same simulation effect. The authors get an acoustic approximation formula, then gain an anisotropic wave equation with 2-order in time and 4-order in space by inverse Fourier transformation, and dispel with the dispersion by adopting the high order finite difference operator in space and remove the edge reflection using the absorption attenuation boundary. Finally, the feasibility and effectiveness of this algorithm can be proved by the snaps and synthetic records.  相似文献   

5.
During the summer of 2011,a severe drought event occurred throughout the Zhujiang(Pearl)River Basin of South China.This decreased runoff into the river,resulting in increased salinity and reduced suspended substance.To examine the effects of this extreme drought on the distribution of nutrients and chlorophyll,we compared two surveys from 2006 and 2011.Results show that dissolved inorganic nitrogen concentration did not change from 2006 to 2011,whereas the proportions of NO 2 – and NH 4 + to DIN in 2011 increased.PO 4 3-concentration was lower in 2011 than in 2006,whereas there was no difference in SiO 3 2-concentration between these years.Correlation coeffi cients of salinity with levels of NO 3 –,NO 2 –,NH 4 +,PO 4 3-and SiO 3 2-in 2011 were all much higher than those in 2006,suggesting greater conservation of dissolved nutrients during the extreme drought event.Furthermore,calculated amounts of regenerated nitrate and phosphorus and their proportions to observed nutrients in 2011 were much lower than in 2006,indicating that nutrient regeneration decreased during the extreme drought period.Mean concentration of chlorophyll a(Chl-a)was considerably higher in 2011 than in 2006,and a harmful algal bloom of Cochlodinium geminatum was observed in the estuary,owing to water stagnancy and lower turbidity as a consequence of drought.Therefore,the extreme drought event in 2011 changed the composition ratio of nutrients,enhanced nutrient conservative behavior,and reduced nutrient regeneration.This affected some key ecological processes in the estuary.  相似文献   

6.
As an important marginal sea under the influences of both the Changjiang River and the Kuroshio, the East China Sea （ECS） environment is sensitive to both continental and oceanic forcing. Paleoenvironmental records are essential for understanding the long-term environmental evolution of the ECS and adjacent areas. However, paleo-temperature records from the ECS shelf are currently very limited. In this study, the U^K_37 and TEX86 paleothermometers were used to reconstruct surface and subsurface temperature changes of the mud area southwest of the Cheju Island （Site F10B） in the ECS during the Holocene. The results indicate that temperature changes of F 10B during the early Holocene （11.6-6.2 kyr） are associated with global climate change. During the period of 6.2-2.5 kyr, the similar variability trends of smoothing average of AT （the difference between surface and subsurface temperature） of Site F10B and the strength of the Kuroshio suggest that the Kuroshio influence on the site started around 6.2kyr when the Kuroshio entered the Yellow Sea and continued to 2.5 kyr. During the late Holocene （2.5-1.45 kyr）, apparent decreases of U^K_37 sea surface temperature （SST） and AT imply that the direct influence of the Kuroshio was reduced while cold eddy induced by the Kuroshio gradually controlled hydrological conditions of this region around 2.5 kyr.  相似文献   

7.
Zhikong scallop （Chlamys farreri） is an important maricultured species in China. Many researches on this species, such as population genetics and QTL fine-mapping, need a large number of molecular markers. In this study, based on the ex- pressed sequence tags （EST）, a total of 300 putative single nucleotide polymorphisms （SNPs） were selected and validated using high resolution melting （HRM） technology with unlabeled probe. Of them, 101 （33.7%） were found to be polymorphic in 48 indi- viduals from 4 populations. Further evaluation with 48 individuals from Qingdao population showed that all the polymorphic loci had two alleles with the minor allele frequency ranged from 0.046 to 0.500. The observed and expected heterozygosities ranged from 0.000 to 0.925 and from 0.089 to 0.505, respectively. Fifteen loci deviated significantly from Hardy-Weinberg equilibrium and significant linkage disequilibrate was detected in one pair of markers. BLASTx gave significant hits for 72 of the 101 poly- morphic SNP- containing ESTs. Thirty four polymorphic SNP loci were predicted to be non-synonymous substitutions as they caused either the change of codons （33 SNPs） or pretermination of translation （1 SNP）. The markers developed can be used for the population studies and genetic improvement on Zhikong scallop.  相似文献   

8.
Zooplankton constitutes a major part of the diet for fish larvae in the marine food web, and it is generally believed that copepods can meet the nutritional requirements of fish larvae. In this study, calanoid copepod Schmackeria poplesia, rotifer Brachionus plicatilis and anostraca crustacean Artemia sp. were analyzed for fatty acid contents, and were used as live food for cul- turing larval Japanese flounder, Paralichthys olivaceus. The total content of three types of HUFAs （DHA, EPA and ARA） in S. po- plesia was significantly higher than that in the other two live foods （P〈O.O1）. Three live organisms were used for raising larvae and juveniles of Paralichthys olivaceus respectively for 15 and 10 d. Then the growth, survival and fatty acid composition of the larvae and juveniles were investigated. The results showed that the larvae and juveniles fed with copepods （S. poplesia） had significantly higher growth rate than those fed with the other two organisms （P〈0.01）. The survival of the flounder larvae fed with copepods was significantly higher than that of the others （P〈0.01）, and the survival of the juvenile fish fed with copepods was higher than that fed with Artemia （P〈0.05）. The contents of three types of HUFAs （DHA, EPA and ARA） and the ratio of DHA/EPA in larval and juve- nile flounder P. olivaceus were analyzed. The results showed that the contents of DHA, EPA and ARA in the larvae and juveniles fed with S. poplesia were higher than those fed with a mixed diet orArtemia only, and the ratio of EPA/ARA in larvae and juveniles of P. olivaceus fed with S. poplesia was lower than that in the case of feeding with a mixed diet or Artemia only. The present data showed that copepod is the best choice for feeding the larvae and juveniles of fish considering its effects on the survival, growth and nutrition composition of the fish.  相似文献   

9.
Polyphenols from the ethanol extracts of Sargassum tenerrimum （ST） with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents： macroporous resin, silica gel, and polyvinylpolypyrrolidone （PVPP）, were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as： with the optimal values shown in brackets, the concen- tration of extract solution （4 times diluted）, pH value （6-7）, adsorption speed （3BVh-1, bed volumes/per hour）, concentration of ethanol （80%）, elution speed （3 BV ht） and elution volume （7 BV）. The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.  相似文献   

10.
Optimal stocking densities were investigated for the sea cucumber Apostichopusjaponicus Selenka under feed-supplement and non-feed-supplement regimes in net enclosures for 333 d. Substantial weight loss occurred during the aestivation phase （AE）. Decreased growth rates were also observed during the winter phase （WT）. In contrast, sea cucumbers showed rapid growth during the spring （SP） and autumn （AU） phases. Feeding regimes considerably influenced the growtb performance, i.e., sea cucumbers grew faster under feed-supplement regime than under non-feed-supplement regime （P 〈 0.05）. The average survival rates of sea cucumbers under feed-supplement regime were higher than those under non-feed-supplement regime for both the autumn phase and spring phase, but the differences were only significant for the latter phase （P〈 0.05）. The fitted B-N curves showed that the optimal stocking densities, in terms of net production, were 22.3 ind. m^-2 for feed-supplement regime and 14.1 ind. m^-2 for non-feed-supplement regime.  相似文献   

11.
In this study we assessed the current status of coral reefs along the Saudi Arabian coast of the Red Sea. Among the three growth forms of Acroporid corals, the branching forms were found to dominate in the Farassan Islands （44.55%±11.10% cover） fol-lowed by tabular forms in the Doga Islands （ranging between 18%±6.47% and 18.30%±9.47% cover）. Digitate forms were rarely found along the coast except at Maqna. Among the five growth forms of non-Acroporid corals, we observed maximum cover of branching forms in the Yanbu offshore area （58.89%±15.11% cover） followed by the Jeddah coast （24.76%±14.04% cover）. The Millepora spp., a non-Scleractinian coral, was abundant at all the near-shore sites, such as Jeddah （10.70%±8.21%） and A1-Wajh （9.81%±6.69%）. The live coral cover （including both Scleractinian and non-Scleractinian corals） of Saudi Red Sea coast was seen to be higher in the north and gradually decrease towards the south. Principal Component analysis showed that the contribution of Acro- porid corals was greater in the southern region than in the northem and middle regions, but vice-versa in the case of non-Acroporid corals. Bray-Curtis cluster analysis categorized all the study sites into two major clusters with 60% similarity. Among them, one cluster-forming sites from Maqna to Masturah （Northem region） and the second one comprised the middle and southem regions （Jeddah to Farassan Islands）, and one outlier Rabigh.  相似文献   

12.
The nitrogen(N) and phosphorus(P) budget and the ecological efficiency of a polyculture system of sea cucumber(Apostichopus japonicus), jellyfish(Rhopilema esculenta) and shrimp(Fenneropenaeus chinensis) were studied in a cofferdam, 120.2 ha in size. The nutrients were supplied by spring tide inflow. In total, 139600 kg N yr-1 and 9730 kg P yr-1 input to the system; while 118900 kg N yr-1 and 2840 kg P yr-1 outflowed from the system concurrently, thus the outflow was 85.7%(N) and 29.2%(P) of inflow. The production of N and P was 889.5 kg yr-1 and 49.28 kg yr-1(sea cucumber) and 204 kg yr-1 and 18.03 kg yr-1(jellyfish and shrimp), respectively. The utilization rate of N and P by polycultured animals was 7.8‰ and 6.9‰, respectively, 21.9% and 38% higher than that of monocultured sea cucumber. Our results indicated that the polyculture system was an efficient culture system of animals and a remediation system of coastal environment as well; it scavenged 14.3% and 70.8% of N and P, respectively. Such an ecological efficiency may be improved further by increasing either the stocking density or the size of sea cucumber or both.  相似文献   

13.
According to topography of Yemen, most areas and villages are located at obligated crest, toe of mountain and under cliffs. Therefore Al-Huwayshah consisting of Tawilah sandstone group is characterized by steep slope reach to 90℃ in some areas. This area is affected by strong tectonic movements and faults that occurred during the geological epochs. This effect enhances to find out fractures and joints as well as the rocks become brittle and ready to slide depending on the position of area. And there are some fractures and joints on the surface of the areas due to tectonic movement associated with opening the Red Sea and Gulf of Aden. The rainfalls, fractures, joints, earthquakes, gravity, vegetations, temperatures and human activities play a big role in the processes of rockfalls and landslides in this area. All those factors are considered as the basic causes and catalyzed factors for occurrence of rockfall in the studide area. In addition, the authors carried out laboratory test for many rock samples to get the physical properties of the rocks.  相似文献   

14.
Chitosan composites and derivatives have gained wide attentions as effective biosorbents due to their low costs and high contents of amino and hydroxyl functional groups. They have showed significant potentials of removing metal ions, dyes and pro- teins from various media. Chemical modifications that lead to the formation of the chitosan derivatives and chitosan composites have been extensively studied and widely reported in literatures. The aims of this review were to summarize the important information of the bioactivities of chitosan, highlight the various preparation methods of chitosan-based active biosorbents, and outline its potential applications in the adsorption of heavy metal ions, dyes and proteins from wastewater and aqueous solutions.  相似文献   

15.
Pyropia yezoensis,an intertidal seaweed,experiences regular dehydration and rehydration with the tides.In this study,the responses of P.yezoensis to dehydration and rehydration under high and low CO 2 concentrations((600–700)×10-6 and(40–80)×10-6,named Group I and Group II respectively)were investigated.The thalli of Group I had a signifi cantly higher effective photosystem II quantum yield than the thalli of Group II at 71% absolute water content(AWC).There was little difference between thalli morphology,total Rubisco activity and total protein content at 100% and 71% AWC,which might be the basis for the normal performance of photosynthesis during moderate dehydration.A higher effective photosystem I quantum yield was observed in the thalli subjected to a low CO 2 concentration during moderate dehydration,which might be caused by the enhancement of cyclic electron fl ow.These results suggested that P.yezoensis can directly utilize CO 2 in ambient air during moderate dehydration.  相似文献   

16.
The toxic effects of benzo[α]pyrene （B[α]P） at different concentrations （0.1, 0.5, 1, 2.5 and 7.5 μgL^-1） on scallop （Chlamys farreri） immune system were studied. The results showed that B[α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial activity （P〈0.05）, while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake, phagocytosis and bacteriolytic activity in all B[α]P treatment groups as well as antibacterial activity in groups of 0.5, 1, 2.5 and 7.5 μgL^-1·B[α]P decreased significantly （P〈0.05）. Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear time- and concentration-response to B[α]E Bactenolytic activity in 0.1 μgL^-1 B[α]P treatment group and antibacterial activity in 0.1 ggLl and 0.5 μgL^-1 B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were significantly lower than those in control group （P〈0.05）, except for antibacterial activity in 0.1 μgLl B[α]P treatment group （P〉0.05）. Thus, B[α]P has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and lmmunomodulation in aquatic organisms.  相似文献   

17.
The authors studied the potential field boundary identification of the new technology in order to find out the possible fractures or contact zones using the following methods such as tilt derivative, horizontal derivative of tilt derivative, normalized standard deviation and normalized differential method. Combined with Euler deconvolution and small subdomain filtering, the actual data processing results show that these methods are all a- ble to identify wider range extending fractures and obtain abundant geological information. The horizontal derivative of tilt derivative and normalized differential method have a better resolution for the small cutting fractures and lacunae in the studied area. They provide a reliable basis for study of the cutting relationship between fractures.  相似文献   

18.
Crustacean zooplankton form the keystone link between primary producers and fish stocks in marine and estuary, ecosystems. We have established a multi-generation cultivation system for zooplankton ruth which future experiments on the biological effects of pollutants in marine and estuary environments can be better performed. A population of calanoid copepod, Sehmakeria poplesia, was collected in December 2003 and maintained in a static system through all stages （eggs to adults）. The population ex- hinted an average developmental time of 13.6 d in conditions corresponding to the natural environment （water temperature 20~C salinity 15）. A series of experiments were performed to examine copepod egg production and hatching success as functions of food type and feeding concentration. Results in our study showed that Isoehrysis galbana was more favored for the reproduction of cope- pods than Phaeodactylum tricornutum, and 10xl0%ellsmL-1 was the most practical algae concentration. We have demonstrated that the Schmakeria poplesia population can be maintained in the laboratory through multiple generations. In addition, methods to control egg production through changes in food concentration have been established, making it feasible to control the start date of exposure experiments or the timing of the collection of oftspring to initiate a new generation.  相似文献   

19.
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20.
The effects of four ions and eight neuroactive compounds on inducing larval settlement ofA. japonicus were assessed in the present study. All bioassays were conducted in 60 × 9mm Petri dishes, each contained 10mL of the test solution and 10 doliolaria larvae. There were significant inductive effects of K＋ （10 mmol L-l）, NH4＋ （0.1 mmol L-l）, GABA （10-3 tool L-l）, acetylcholine （10-5 molL-l）, L-DOPA （10-SmolL-1）, norepinephrine （10-SmolL-1） and dopamine （10-TmolL-1 and 10-5 molL-1） on the settlement of sea cucumber larvae. L-DOPA and dopamine are the most efficient chemical cues to induce A.japonicus larvae to settle. The highest percentage of larval settlement was induced by 10-5 tool L-1 L-DOPA and dopamine （33% and 40%） compared to the control （7%）. However, Ca2＋, Mg2＋, choline, serotonin, and epinephrine were less effective on larval settlement at all tested concentrations. This study evaluated the stability and feasibility of chemical cues for larval settlement in different culture systems, which can be applied to improve the hatchery production of this valuable species.  相似文献   

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