共查询到16条相似文献，搜索用时 281 毫秒
1.
哲罗鱼(Hucho taimen)染色体组型与DNA含量分析 总被引：1，自引：0，他引：1
采用体内注射PHA和秋水仙素、肾细胞短期培养、常规空气干燥法制备哲罗鱼(Hucho taimen)的染色体。对其肾细胞染色体数目统计分析表明,哲罗鱼染色体组有84条染色体,核型公式为2n=18m+16sm+34st+16t,其染色体总臂数(NF)为118。采用流式细胞分析仪测定哲罗鱼的DNA含量,与鸡血细胞标准对照的比值为2.23±0.17,以鸡红细胞DNA含量2.30pg·N~(-1)计,则哲罗鱼的体细胞DNA含量为5.12pg·N~(-1)。哲罗鱼染色体数目和DNA含量体现为四倍体特征。 相似文献
2.
利用193nm准分子激光剥蚀系统（LA）,联用多接收电感耦合等离子体质谱仪（MCICPMS）,在激光频率为2Hz,束斑直径为24μm条件下,对91500、GJ1、TEMORA1、Pleovice和Qinghu等5个标准锆石进行了原位UPb年龄测定。测试结果显示206Pb/238U加权平均年龄分别为(1062.3±4.3)Ma（2σ,n=21）,(604.8±3.1)Ma（2σ,n=20）,(418.3±1.6)Ma（2σ,n=27）,(337.3±1.3)Ma（2σ,n=28）,(159.86±0.64)Ma（2σ,n=28）,207Pb/206Pb、206Pb/238U、207Pb/235U比值的分析精度（2σ）2%左右,加权平均年龄的分析精度（2σ）均小于0.5%。上述结果表明,91500、GJ1、TEMORA1、Pleovice和Qinghu等5个标准锆石的加权平均年龄在误差范围内与前人报道完全一致。对产在胶东玲珑花岗岩中的实际锆石样品JD47进行测试,所获得的206Pb/238U加权平均年龄为(154.3±2.1)Ma,与SHRIMP测试结果吻合。文中建立的24μm锆石LAMCICPMS UPb年龄测试方法,与LAICPMS方法相比,测试精度高,剥蚀时间短,激光剥蚀空间分辨率提高。 相似文献
3.
斜带石斑鱼与赤点石斑鱼的核型研究 总被引：7，自引：0，他引：7
采用PHA体内注射肾细胞直接制片法研究斜带石斑鱼和赤点石斑鱼的核型。结果表明 ,斜带石斑鱼的核型为 2n =4 8,2sm +46t ,NF =5 0 ;赤点石斑鱼的核型为 2n =4 8,5st+43t ,NF =4 8。两种鱼的 2n数相同 ,它们的核型特点符合典型的高位类群鱼类核型特征。 相似文献
4.
利用实验生态学的方法研究不同温度(15、20、25、30和35℃)、盐度(0、5、10、15和20)、pH(5、6、7、8和9)和体质量(1.93±0.08、3.43±0.12、7.95±0.34、11.74±0.41和15.84±0.61 g)对尼罗罗非鱼幼鱼窒息点的影响,并建立温度、盐度、pH、体质量与罗非鱼窒息点间的回归方程。结果表明:温度t、盐度S、pH和体质量m对尼罗罗非鱼幼鱼窒息点有显著影响(P<0.05)。当温度、盐度、pH和体质量分别为25.28℃、3.6、8.06、6.3 g时,窒息点(DAP)最低,回归方程为DAP=0.000 9 t 2-0.045 5 t+0.726 6(R2=0.952 1),DAP=-0.000 1 S 3+0.004 2 S 2-0.023 9 S+0.111 9(R2=0.922 8),DAP=0.009 3 pH2-0.149 9 pH+0.7174(R2=0.976 8);DAP=-0.000 3 m3+0.011 1 m2-0.104 3 m+0.362 8(R2=0.948 4)。在溶氧不足情况下,温度25.28℃、盐度3.6、pH 8.06的环境条件有助于提高幼鱼耐低氧能力。 相似文献
5.
双棘黄姑鱼血清性类固醇激素的周年变化 总被引：1，自引：0，他引：1
采用放射免疫法(RIA)测定双棘黄姑鱼(Nibea diacanthus)血清性类固醇激素的周年变化。结果表明:雌鱼血清雌二醇(Estadiol-17β)浓度一年出现两次峰值,均在成熟产卵前一个月,即4月[(338±59)pg/mL]和9月[(296±48)pg/mL];血清睾酮(T)浓度在卵黄发生期急剧上升,并在卵巢成熟期达到最高值,分别是5月(2.506±0.262 ng/mL)和10月份(2.268±0.243 ng/mL),分别在血清雌二醇浓度两次峰值出现1个月之后,因此,雌鱼的血清雌二醇浓度和血清睾酮浓度均与性腺发育相关。雄鱼血清睾酮浓度的两次峰值均出现在性腺已发育成熟的5月份(2.307±0.792 ng/mL)和10月份(2.286±0.607 ng/mL),与性腺发育有明显的相关性;而血清雌二醇浓度全年维持在一个相对稳定的水平,与雄性性腺发育相关性不明显。 相似文献
6.
乌苏里江唇耗氧率和窒息点的初步研究 总被引：2，自引：0，他引：2
进行不同温度和不同规格人工养殖的乌苏里江唇(Hemibarbus labeo)耗氧量(MO2)、耗氧率(WO2)和窒息点(SO2)的初步测定。结果表明:水温16~28℃范围内,随着水温的升高,耗氧率、耗氧量和窒息点都表现出增大的变化趋势。温度和耗氧率的回归方程:1龄鱼体重11 g的为WO2=0.0142T-0.0863(R2=0.9617),2龄鱼体重60 g的为WO2=0.0138T-0.1121(R2=0.97);温度和窒息点的回归方程:1龄鱼体重8 g的为SO2=0.0298T-0.1891(R2=0.977 8),2龄鱼体重40 g的为SO2=0.048 8T-0.402 5(R2=0.999 7)。体重20~100 g范围内,水温25℃时,随着体重规格的增大,耗氧量逐渐升高,耗氧率逐渐减小。体重和耗氧率的回归方程为WO2=0.7825W-0.2898(R2=0.947 4),体重和耗氧量的回归方程为MO2=0.7837W0.7097(R2=0.991 6)。唇鱼的昼夜耗氧率(水温25℃,体重72 g)有一定的变化趋势,早间4~10时耗氧率较高,平均在0.332±0.046 mg/(g.h),午间11~19时中等,在0.287±0.030 mg/(g.h),晚间20~3时较低,在0.251±0.029 mg/(g.h)。 相似文献
7.
进行不同温度和不同规格人工养殖的乌苏里江唇（Hemibarbus labeo）耗氧量（MO2）、耗氧率（WO2）和窒息点（SO2）的初步测定。结果表明：水温16-28℃范围内,随着水温的升高,耗氧率、耗氧量和窒息点都表现出增大的变化趋势。温度和耗氧率的回归方程：1龄鱼体重11 g的为WO2=0.0142T-0.0863（R2=0.9617）,2龄鱼体重60 g的为WO2=0.0138T-0.1121（R^2=0.97）;温度和窒息点的回归方程：1龄鱼体重8 g的为SO2=0.0298T-0.1891（R^2=0.977 8）,2龄鱼体重40 g的为SO2=0.048 8T-0.402 5（R^2=0.999 7）。体重20-100 g范围内,水温25℃时,随着体重规格的增大,耗氧量逐渐升高,耗氧率逐渐减小。体重和耗氧率的回归方程为WO2=0.7825W-0.2898（R2=0.947 4）,体重和耗氧量的回归方程为MO2=0.7837W0.7097（R^2=0.991 6）。唇鱼的昼夜耗氧率（水温25℃,体重72 g）有一定的变化趋势,早间4-10时耗氧率较高,平均在0.332±0.046 mg/（g.h）,午间11-19时中等,在0.287±0.030 mg/（g.h）,晚间20-3时较低,在0.251±0.029 mg/（g.h）。 相似文献
8.
本文对我国研制的ZYZY型海洋重力仪弹性系统的结构和工作原理作了论述。研究了消除各种外界干扰的影响,并以实验数据说明了仪器的各种性能指标。文章最后详细地叙述了海上三次测量时的海况和测量的精度。第一航次:ZY№1 m=±1.92毫伽,ZY№2m=±3.05毫伽;第二航次:ZY№1m=±1.69毫伽,ZY№2m=±2.40毫伽;第三航次:ZY№1m=±2.52毫伽,ZY№2m=±2.05毫伽。 相似文献
9.
粤西福建纹胸鮡的食性及繁殖力 总被引：1，自引：0，他引：1
对2007年取自粤西水域的福建纹胸鮡进行生物学研究。结果显示:粤西福建纹胸鮡食性是以动物性食物为主;雌雄性比为1.16∶1;性腺成熟系数雌性为0.263%～19.048%,雄性为0.101%～3.226%;福建纹胸鮡个体绝对怀卵量在45～420粒之间,平均140.9±74.7粒;相对怀卵量在31.7～130.4粒/g之间,平均64.3±21.1粒/g;绝对怀卵量与体长呈幂相关:y=0.0008 x7.7308,R2=0.855 2;绝对怀卵量与体重呈指数相关:y=14.439 e0.838 2 x,R2=0.878 3。 相似文献
10.
乐茂华 《广东海洋大学学报》2004,24(4):47-48
对于正整数a ,设S(a)是Smarandache函数。证明了 :方程S(1·2 ) +S(2·3) +… +S(x(x +1) ) =S(x(x +1) (x +2 ) /3)仅有正整数解x =1。 相似文献
11.
管角螺、细角螺的核型研究 总被引：2，自引：0，他引：2
以管角螺、细角螺鳃组织为材料,经过秋水仙素处理,空气干燥法制片,Giemsa染色,观察分析获得染色体核型。结果表明,管角螺核型公式为2n=60=44m+10sm+6t,染色体总臂数NF=114。细角螺核型公式为2n=42m+10sm+2st+6t,总臂数NF=112。两种螺的全套染色体中未发现次缢痕、随体,也无性染色体。 相似文献
12.
We performed a karyotype analysis on four species from Phylum Nemertea using regenerating somatic cells. Two palaeonemertean species, Cephalothrix hongkongiensis and Cephalothrix sp., had the same chromosome number (2n=28), but different karyotypes (16 m+10 sm+2 st, NF=54 and 22 m+4 sm+2 st, NF=54, respectively). The karyotypes of the two heteronemerteans, Notospermus geniculatus and Ramphogordius sanguineus were 2n=38, 30 m+6 sm+2 sm/st (NF=76/74) and 2n=56, 48 m+4 sm+4 sm/st (NF=112/108), respectively. 相似文献
13.
The marine chroococooid phycoerythrin-containingSynechococcus spp. cyanobacterium has been implicated as a substantial component of the photosynthetic picoplankton in the ocean. Although
its importance as food source for heterotrophic nanoplankton is now recognized, information about the cycling ofSynechococcus biomass and its diel pattern is limited and study methodology varies among authors. The selective metabolic inhibitor method
was used to simultaneously estimate growth and grazing disappearance rates ofSynechococcus in the English Channel where growth rates ranged from 0.25 to 0.72/d (mean ±SD=0.51±0.17/d) and grazing mortality rates ranged
from 0.19 to 0.64/d (mean ±SD=0.48±0.17/d). Size-fractionated experiments demonstrated that up to 70% ofSynechococcus disappearance could be attributed to grazers going through a 2 μm Nuclepore filter.Synechococcus grazing mortality rates (mean=0.74 ±0.25/d) during the day were always higher than that (mean=0.2±0.20/d) during the night,
while growth rates showed no clear diel pattern. A positive correlation was observed between growth rates andin situ temperature ranging from 9 to 17°C, while in contrast grazing was independent of temperature. The close similatiry between
average growth and grazing rates suggests a rapid recycling ofSynechococcus biomass in English Channel coastal waters. 相似文献
14.
The International Eq一lation of State of Seawater,1980 and the PraeticalSalinity Scale,1978 have been adoPted by theUNESCO江CES沼COR八APSOJoint Panel ono‘eanogral,hie Tables and Standards(JPOTS),and endorsed bythese organizati6ns(Miller‘〕and Poisson,1981;Uneseo,1981).Th已new equa-tion and the Praetieal Salinity Seale are to be used for all values Published fromJan .1,1982 .The new equation 15 aeeurate for use in all oeeanie surfaee waters,but eannot be aeeurately aPPlied to… 相似文献
15.
Japanese flounder is one of the most important commercial species in China; however, information on the genetic background of natural populations in China seas is scarce. The lack of genetic data has hampered fishery management and aquaculture development programs for this species. In the present study, we have analyzed the genetic diversity in natural populations of Japanese flounder sampled from the Yellow Sea (Qingdao population, QD) and East China Sea (Zhoushan population, ZS) using 10 polymorphic microsatellite loci and cytochrome c oxidase subunit I (COI) sequencing data. A total of 68 different alleles were observed over 10 microsatellite loci. The total number of alleles per locus ranged from 2 to 9, and the number of genotypes per locus ranged from 3 to 45. The observed heterozygosity and expected heterozygosity in QD were 0.733 and 0.779, respectively, and in ZS the heterozygosity values were 0.708 and 0.783, respectively. Significant departures from Hardy-Weinberg equilibrium were observed in 7 of the 10 microsatellite loci in each of the two populations. The COI sequencing analysis revealed 25 polymorphic sites and 15 haplotypes in the two populations. The haplotype diversity and nucleotide diversity in the QD population were 0.746±0.072 8 and 0.003 34±0.001 03 respectively, and in ZS population the genetic diversity values were 0.712±0.047 0 and 0.003 18±0.000 49, respectively. The microsatellite data (F_st =0.048 7, P<0.001) and mitochondrial DNA data (F_st =0.128, P<0.001) both revealed significant genetic differentiation between the two populations. The information on the genetic variation and differentiation in Japanese flounder obtained in this study could be used to set up suitable guidelines for the management and conservation of this species, as well as for managing artificial selection programs. In future studies, more geographically diverse stocks should be used to obtain a deeper understanding of the population structure of Japanese flounder in the China seas and adjacent regions. 相似文献
16.
【目的】研究棕点石斑鱼(Epinephelus tukula,♀)与蓝身大斑石斑鱼(Epinephelus fuscoguttatus,♂)杂交F1代(下称"金虎斑")的染色体核型。【方法】采用头肾-PHA注射法制备金虎斑染色体,并分析其核型。【结果与结论】金虎斑二倍体染色体数为48,包括23对端部着丝粒染色体,1对亚中部着丝粒染色体,核型公式是2n=48=2sm+46t,染色体臂数(NF)为50。与其他石斑鱼核型相比,金虎斑染色体核型特点符合典型的高位类群鱼类核型特征;根据染色体臂数,金虎斑属于特化类群。 相似文献