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1.
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2.
The sulfate-reducing bacteria (SRB) community in the deep-sea sediments of the west Pacific Warm Pool (WP) was surveyed by molecular phylogenetic analyses using primers targeting the 16S rRNA gene fragments of SRB. Specific 16S rRNA gene libraries from five sediment layers (1-cm, 3-cm, 6-cm, 10-cm and 12-cm layer) of the 12-cm core of WP-0 were constructed. The clones in the five libraries were differentiated by restriction fragment length polymorphism (RFLP) and representative clones were selected to sequence. It was found that the clones fell into four groups, which were closest related to Desulfotomaculum, Desul- facinum, Desulfomonile and Desulfanuticus. Desulfacinum-like clones were only detected in the upper layers of the sediment core, whereas Desulfomonile-like clones were only present in the deeper layers. Fluorescence in situ hybridization (FISH) was further carried out to visualize and count the SRB and bacteria in the five sediment layers. It was found that SRB constituted only a small proportion of the bacteria community (0.34%-1.95%), it had the highest content in the 3-cm layer (1.95%) and had a depth- related decreasing tendency along the 12- cm core.  相似文献   

3.
Relationships between microbial communities and geochemical environments are important in marine microbial ecology and biogeochemistry. Although biogeochemical redox stratification has been well documented in marine sediments, its impact on microbial communities remains largely unknown. In this study, we applied denaturing gradient gel electrophoresis (DGGE) and clone library construction to investigate the diversity and stratification of bacterial communities in redox-stratified sandy reef sediments in a microcosm. A total of 88 Operational Taxonomic Units (OTU) were identified from 16S rRNA clone libraries constructed from sandy reef sediments in a laboratory microcosm. They were members of nine phyla and three candidate divisions, including Proteobacteria (Alpha-, Beta-, Gamma-, Delta-, and Epsilonproteobacteria), Actinobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Firmicutes, Verrucomicrobia, Spirochaetes, and the candidate divisions WS3, SO31 and AO19. The vast majority of these phylotypes are related to clone sequences from other marine sediments, but OTUs of Epsilonproteobacteria and WS3 are reported for the first time from permeable marine sediments. Several other OTUs are potential new bacterial phylotypes because of their low similarity with reference sequences. Results from the 16S rRNA, gene clone sequence analyses suggested that bacterial communities exhibit clear stratification across large redox gradients in these sediments, with the highest diversity found in the anoxic layer (15–25 mm) and the least diversity in the suboxic layer (3–5 mm). Analysis of the nosZ, and amoA gene libraries also indicated the stratification of denitrifiers and nitrifiers, with their highest diversity being in the anoxic and oxic sediment layers, respectively. These results indicated that redox-stratification can affect the distribution of bacterial communities in sandy reef sediments.  相似文献   

4.
Li  Yingjie  Cao  Wenrui  Wang  Yan  Ma  Qingjun 《中国海洋湖沼学报》2019,37(3):1024-1029
Microbial diversity in the abyssal sediments beneath the seafloor of 30, 94, and 151 cm near the southern end of the Mariana Trench was analyzed in the Illumina HiSeq 2500 platform. Results show that the microbial populations were dominated by bacteria but merely no archaea were identified at the three depths. In the bacterial community,Proteobacteria and Firmicutes dominated the total taxon tags, followed by Bacteroidetes, Actinobacteria, Planctomycetes, Cyanobacteria, and Chloroflexi, which together account for over 99% of the total population. Similar to that in the seawater in the trench, the operational taxonomic units(OTUs) belonging to Gammaproteobacteria from the sediment samples showed high abundance.However, common bacterial OTUs in the water of the trench including Nitrospirae and Marinimicrobia were hardly found in the sediments from the southern Mariana Trench or the hadal region. Therefore, this study documented for the first time the compositions of microbial diversity in the trench sediments, revealed the difference in microbial diversity in water and sediment of the trench and will enrich the knowledge on the microbial diversity in the abyssal areas.  相似文献   

5.
To study the compaction law and overpressure evolution in deepwater shallow sediments, a large-strain compaction model that considers material nonlinearity and moving boundary is formulated. The model considers the dependence of permeability and material properties on void ratio. The modified Cam-Clay model is selected as the constitutive relations of the sediments, and the deactivation/reactivation method is used to capture the moving top surface during the deposition process. A one-dimensional model is used to study the compaction law of the shallow sediments. Results show that the settlement of the shallow sediments is large under their own weight during compaction. The void ratio decreases strictly with burial depth and decreases more quickly near the seafloor than in the deeper layers. The generation of abnormal pressure in the shallow flow sands is closely related to the compaction law of shallow sediments. The two main factors that affect the generation of overpressure in the sands are deposition rate and permeability of overlying clay sediments. Overpressure increases with an increase in deposition rate and a decrease in the permeability of the overlying clay sediment. Moreover, an upper limit for the overpressure exists. A two-dimensional model is used to study the differential compaction of the shallow sediments. The pore pressure will still increase due to the inflow of the pore fluid from the neighboring clay sediment even though the deposition process is interrupted.  相似文献   

6.
At present, the understanding of the dynamics of denitrifiers at different dissolved oxygen (DO) layers under organic carbon consumption within the surface sediments remains inadequate. In this study, high-throughput sequencing and quantitative PCR targeting nirS gene were used to analyze the denitrifier abundance dynamics, community composition, and structure for aerobic (DO 0.5–6.9 mg/L), hypoxic-anoxic (DO 0–0.5 mg/L), and anoxic (DO 0 mg/L) layers in surface sediments under organic carbon consumption. Based on the analysis of nirS gene abundance, the values of denitrifying bacteria decreased with organic carbon consumption at different DO layers. When the bacterial species abundance at the genus level were compared between the high-carbon and low-carbon sediments, there was significant increase in 6 out of 36, 7 out of 36 and 6 out of 36 genera respectively for the aerobic, hypoxic-anoxic and anoxic layers. On the other hand, 14 out of 36, 9 out of 36 and 15 out of 36 genera showed significant decrease in bacterial species abundance respectively for the aerobic, hypoxic-anoxic and anoxic layers. Additionally, 14 out of 36, 20 out of 36, and 15 out of 36 genera had no change in bacterial species abundance respectively for the aerobic, hypoxic-anoxic, and anoxic layers. This indicates that the carbon utilization ability of different denitrifiers on each DO layers was generally different from each other. Diversity of denitrifying bacteria also presented significant differences in different DO layers between the high- and low-carbon content sediment layers. Moreover, under the high-carbon and low-carbon content, the abundance of nirS gene showed a high peak within the hypoxic-anoxic regions, suggesting that this region might be the main distribution area for the denitrifying bacteria within the surface sediments. Furthermore, community of unique denitrifiers occurred in different DO layers and the adaptive changes of the denitrifier community followed the organic carbon consumption.  相似文献   

7.
Ecological purification in a reservoir is an important strategy to control the level of nutrients in water.The bacterial community of such a reservoir is the main agent for pollutant degradation,which has not been fully documented.Taking the Jinze Reservoir,a freshwater source for Shanghai,China as the case,its spatial distributions of water and sediment bacteria were determined using 16 S rRNA gene-based Illumina MiSeq sequencing,and the environmental parameters were analyzed.The reservoir takes natural river water and functions as an ecological purification system,consisting of three functional zones,i.e.,pretreatment zone,ecological purification zone,and ecological sustaining zone.Results show that the concentrations of both total nitrogen(TN) and total phosphorus(TP) decreased considerably after the ecological treatment,and the concentration of dissolved oxygen(DO) in the ecological purification zone was boosted from that before pretreatment.In addition,patterns of bacterial communities in both water and sediment were similar and consisted of mainly Proteobacteria,Actinobacteria,and Bacteroidetes.However,difference in water bacterial composition was distinct in each functional zone,whereas the bacterial communities in sediment changed only slightly among sediment samples.Network analysis revealed nonrandom co-occurrence patterns of bacterial community composition in water and sediment,and Pseudomonas,unclassified Comamonadaceae,Variovorax,and Dechloromonas were the key taxa in the co-occurrence network.The bacterial taxa from the same module of the network had strong ecological connections,participated in C-cycles,and shared common trophic properties.PICRUSt analysis showed that bacteria were involved potentially in various essential processes;and the abundance of predicted xenobiotic biodegradation genes showed a decreasing trend in water samples from the inlet to the outlet of the reservoir.These results improve our current knowledge of the spatial distribution of bacteria in water and sediment in ecological purification reservoirs.  相似文献   

8.
Fluorescence in-situ hybridization （FISH） and 16S rRNA gene clone library analyses were used to determine the abundance and diversity of archaea in Prydz Bay, Antarctica. Correlation analysis was also performed to assess links between physicochemical parameters and archaeal abundance and diversity within the sea-ice. Samples of sea-ice and seawater were collected during the 26th Chinese National Antarctic Research Expedition. The results of FISH showed that archaea were relatively abundant within the top layer of the sea-ice, and correlation analysis suggested that the concentration of 4NH＋ might be one of the main factors underlying this distribution pattern. However, using 16S rRNA gene libraries, archaea were not detected in the top and middle layers of the sea-ice. All archaeal clones obtained from the bottom layer of the sea-ice were grouped into the Marine Group I Crenarchaeota while the archaeal clones from seawater were assigned to Marine Group I Crenarchaeota, Marine Group II Euryarchaeota, and Marine Group III Euryarchaeota. Overall, the ifndings of this study showed that the diversity of archaea in the sea-ice in Prydz Bay was low.  相似文献   

9.
Yan  Muting  Chen  Xiaofeng  Chu  Wei  Li  Weixin  Li  Minqian  Cai  Zeming  Gong  Han 《中国海洋湖沼学报》2023,41(1):215-228

The microbial communities colonized on microplastics (MPs) have attracted widespread attention. However, few studies focused on the MPs impacts on mangrove ecosystems, particularly on bacterial communities. We investigated the MPs pollution in mangrove of Zhujiang (Pearl) River estuary (ZRE). To study the potential risk posed by MPs to the mangrove ecosystems, the differences in bacterial communities, functions, and complexity between MPs and sediment samples were reported for the first time. Microplastics (2 991±1 586 items/kg dry weight (dw)) in sediment were mainly fibers and polyethylene, mostly transparent, and in size less than 0.5 mm. Bacterial communities and functions significantly differed from MPs in mangrove sediment. Compared with sediment, MPs significantly enriched members of Proteobacteria, Bacteroidetes, and Actinobacteria, as well as the bacteria associated with plastic-degrading and human diseases on their surface, suggesting that microbial communities on MPs may promote MPs degradation and the spread of diseases, posing potential risk to mangrove ecosystems and human health. Although bacteria on MPs exhibited a lower diversity, the co-occurrence network analysis indicated that network of bacteria colonized on MPs was bigger and more complex than those of mangrove sediment, illustrating that MPs can act as a distinct habitat in this special ecosystem. This study provides a new perspective for increasing our understanding of microplastic pollution in mangrove ecosystems.

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10.
Studies on the diversity and distribution of bacterial populations will improve the overall understanding of the global patterns of marine bacteria and help to comprehend local biochemical processes and environments. We evaluated the composition and the dynamics of bacterial communities in the sediment of Jiaozhou Bay (China) using PCR-denaturing gradient gel electrophoresis (DGGE). Sediment samples were collected from 10 different sites in May, August, and November 2008 and in February 2009. There was significant temporal variation in bacterial community composition at all sites. However, the spatial variation was very small. The DGGE analyses of bacterial communities were used to divide the 10 stations into three types. Canonical correspondence analysis (CCA) revealed that the changes in bacterial communities were driven by sediment properties. Sequence analysis of DGGE band-derived 16S rRNA gene fragments revealed that the dominant bacterial groups in the sediment were of the classes γ-proteobacteria and δ-proteobacteria and phyla Bacteroidetes and Nitrospirae. Our results provide considerable insight into the bacterial community structure in Jiaozhou Bay, China.  相似文献   

11.
For revealing the effects of increasing of zinc oxide nanoparticles(ZnO NPs) on denitrification and denitrifying bacteria communities in estuarine sediments, the surface sediments of two typical estuaries(the Yangtze River Estuary and the Yellow River Estuary) were added with medium concentration(170 mg L~(-1)) and high concentration(1700 mg L~(-1)) of ZnO NPs for anaerobic culture in laboratory. The concentration of NO_3~-and NO_2~-, the reductase activity and denitrification rate were measured by physicochemical analysis, nir S gene abundance and denitrifying bacteria communities by molecular biological methods. The results showed that ZnO NPs inhibited NO_3~-, NO_2~-reduction process and NO_3~-, NO_2~-reductase activity, and a stronger inhibition effect resulting from the higher ZnO NPs concentration. ZnO NPs decreased nirS gene abundance and community diversity of denitrifying bacteria. In addition, the inhibition degree of ZnO NPs on the denitrification process of sediments in different estuaries was different. These results were of great significance for evaluating the potential ecological toxicity and risks of nanomaterials in estuaries.  相似文献   

12.
Straits are ideal models to investigate the bacterial community assembly in complex hydrological environments. However, few studies have focused on bacterial communities in them. Here, comparable bacterial communities in costal shallow Bohai Strait(BS) and oceanic deep Fram Strait(FS) were studied. The Shannon and Chao1 indices were both higher in BS than in FS. The relative abundances of the classes Deltaproteobacteria and Bacilli and the family Halieaceae were higher in BS than in FS, in contrast to the families OM1_clade and JTB255_marine_benthic_group, revealing typical characteristics of bacterial communities in coastal and oceanic regions. Cluster analysis based on the Bray-Curtis index showed that samples were clustered by depth layer in FS and BS, indicating that structures of bacterial communities would diff er with increasing water depth in straits. Additionally, the cluster relationships among samples in abundant and rare communities were both similar to those in entire communities. However, the dissimilarities among samples showed a descending order as rare communities, entire communities and abundant communities. Network analysis indicated that the BS network was obviously more complex than the FS network. Filamentous bacteria Desulfobulbaceae exhibited high degree values in BS but not in FS, indicating key roles of Desulfobulbaceae in the BS. Our study provides different and common evidences for understanding microbial ecology in coastal shallow and oceanic deep straits.  相似文献   

13.
1　IntroductionInthedeepseawithawatertemperaturelowerthan5℃andtheseabottom ,theor ganicmattersareoxidizedmainlybytheaerobicbacteria (BenderandHeggie 1 984;Her bert 1 986) ,whichmeansthattheaerobicbacteriainthisenvironmentareratherabun dant.Thearcticseaareaisanareawhereenvironmentispermanentlycold ,butsomere searchresults (Sahmetal.1 998)indicatethattherearedominantbacteria eubacteriainthearcticseawaterandsediment,andtheiradaptationtotemperaturehasnosubstantialdifferenceincomparisonwiththatof…  相似文献   

14.
The wetlands on the Zoige Plateau have experienced serious degradation, with most of the original marsh being converted to marsh meadow or meadow. Based on the 3 wetland degradation stages, we determined the effects of wetland degradation on the structure and relative abundance of nitrogen-cycling (nitrogen-fixing, ammonia-oxidizing, and denitrifying) microbial communities in 3 soil types (intact wetland: marsh soil; early degrading wetland: marsh meadow soil; and degraded wetland: meadow soil) using 454-pyrosequencing. The structure and relative abundance of nitrogen-cycling microbial communities differed in the 3 soil types. Proteobacteria was the predominant phylum in most soil samples but the most abundant soil nitrogen-fixing and denitrifying microbial bacteria differed at the class, order, family, and genus levels among the 3 soil types. At the genus level, the majority of nitrogen-fixing bacterium sequences related to Bradyrhizobium were from marsh and marsh meadow soils; whereas those related to Geobacter originated from meadow soil. The majority of ammonia-oxidizing bacterium sequences related to Nitrosospira were from marsh (except for the 40-60 cm layer), marsh meadow and meadow soils; whereas those related to Candidatus Solibacter originated from 40-60 cm layer of marsh soil. The majority of denitrifying bacterium sequences related to Candidatus Solibacter and Anaeromyxobacter were from marsh and meadow soils; whereas those related to Herbaspirillum originated from meadow soil. The distribution of operational taxonomic units (OTUs) and species were correlated with soil type based upon Venn and Principal Coordinates Analysis (PCoA). Changes in soil type, caused by different water regimes were the most important factors influencing compositional changes in the nitrogen-fixing, ammonia-oxidizing, and denitrifying microbial communities.  相似文献   

15.
Seamounts are subsurface mountains in the ocean.Examination of the abundance and distribution of Archaea in seamount ecosystems may provide a better understanding of their ecological functions.Most studies of marine archaeal assemblages in seamount area have focused on hydrothermal vents or ferromanganese crusts.We investigated the archaeal communities from a seamount of the Mariana Volcanic Arc,in the tropical western Pacific Ocean by using high-throughput sequencing.Thaumarchaeota was dominant in the sediments of all sample stations.Community diversity and species richness were greatest at stations near the top of the seamount,and lowest at the deepest station.One sample station on the steep southeast slope that faced the Yap-Mariana trench had a unique composition of Archaea.In summary,depth has an important influence on archaeal community structure,and the geographic properties and sediment characteristics may explain the unique distribution patterns of Archaea in this seamount.This study provides a foundation for future research on Archaea in seamounts.  相似文献   

16.
INTRODUCTIONChrbondioxideandInehanearethetwomostabundantabosphericcarbonspedes.Methane'sconcenhati0nintheboposphereinonsesO.7-l.l%peryearraasmussenandKhaili,l98l,BlakeandRowand,l988,Scheeetal.,l989).Ihauseofmethne'sforpaCtontheearth'sclirnateandthechernistryoftheatInosphere,thebudgetofabosphericmethanehasmivedconsiderableattchti0n.Wetlandsareestirnataltobeoneofthelamptsoimofabosphericmethane,aocountingforab0ut4O%to5O%0ftheglobalInehanesoonannually(Cforneand0reInland,l988,WhitingandC…  相似文献   

17.
Many evidences for gas hydrate bearing sediments had been found in the continental slope of the East China Sea, such as bottom simulating reflections(BSRs), undersea gas springs, pyrite associated with methane leakage, mud diapirs/mud volcanos, bottom-water methane anomalies and so on. In this study, six key stratigraphic interfaces including T_0(seafloor), T_1(LGM, 23 kyr B.P.), T_2(2.58 Myr), T_3(5.33 Myr), T_4(11.02 Myr) and T_5(16.12 Myr) were identified, and then five third-order sequences of SQIII1 to SQIII5 were divided. However, T5 in southern continental slope is not found, which shows that the middle-northern Okinawa Trough had begun to rift in the early Miocene, earlier than the southern segment. Four system tracts including lowstand systems tract(LST), transgressive systems tract(TST), highstand systems tract(HST) and falling stage systems tract(FSST) are further divided. The marine erosion interface of 11.02 Myr and regressive unconformity interface of 23 kyr B.P. indicate two large-scale sea level drop events in the research area. Seven typical seismic facies identified in the continental slope are continental shelf-edge deltas, littoral fluvial-delta plains, incised channels or submarine canyons, slope fans, submarine fans or coastal sandbars, littoral-neritic finegrained sediments, mud volcanos and some other geological bodies respectively. The minimum water depth for hydrate occurrence in the Okinawa Trough is 630 m, and the thickness of gas hydrate stability zone in continental slope is between 0 and 590 m. The calculated bottom boundary of hydrate stability zone is slightly deeper than BSRs on the seismic sections. The re-depositional turbidite sand bodies, such as canyon channels, slope fans and submarine fans developed in Quaternary strata, are the predominant hydrate reservoirs. According to developing process, the dynamic accumulation of hydrate systems can be divided into three evolutionary stages including canyon erosion and hydrate stability zone migration stage, sediments destabilizing and methane leakage stage, and channel filling and hydrate re-occurrence stage.  相似文献   

18.
Leaves of terrestrial and aquatic plants are home to a wide diversity of bacterial species. However, the diversity and variability of epiphytic bacteria on their submerged plant hosts remains poorly understood. We investigated the diversity and composition of epiphytic bacteria from two common submerged macrophytes: Vallisneria natans and Hydrilla verticillata in Taihu Lake, Jiangsu, China, using methods of terminal restriction fragment length polymorphisms (T-RFLP) and clone library analyses targeted at bacterial 16S rRNA genes. The results show that: (1) the libraries of the two waterweeds contain wide phylogenetic distribution of bacteria, and that the sequences of the two libraries can be separated into 93 OTUs (at 97% similar value); (2) Betaproteobacteria, including Burkholderiales, was the most abundant bacterial group on both plants. Cyanobacteria and Gammaproteobacteria were the second largest groups on V. natans and H. verticillata, respectively. Both clone libraries included some sequences related to those of methanotrophs and nitrogen-fixing bacteria; (3) Cluster analysis of the T-RFLP profiles showed two distinct clusters corresponding to the two plant populations. Both ANOSIM of the T-RFLP data and Libshuff analysis of the two clone libraries indicated a significant difference in epiphytic bacterial communities between the two plants. Therefore, the epiphytic bacterial communities on submerged macrophytes appear to be diverse and host-specific, which may aid in understanding the ecological functions of submerged macrophytes in general.  相似文献   

19.
Based on the stratigraphic sequence formed since the last glaciation and revealed by 3000 km long high-resolution shallow seismic profiles and the core QDZ03 acquired recently off the southern Shandong Peninsula, we addressed the sedimentary characteristics of a Holocene subaqueous clinoform in this paper. Integrated analyses were made on the core QDZ03, including sedimentary facies, sediment grain sizes, clay minerals, geochemistry, micro paleontology, and AMS 14 C dating. The result indicates that there exists a Holocene subaqueous clinoform, whose bottom boundary generally lies at 15–40 m below the present sea level with its depth contours roughly parallel to the coast and getting deeper seawards. The maximum thickness of the clinoform is up to 22.5 m on the coast side, and the thickness contours generally spread in a banded way along the coastline and becomes thinner towards the sea. At the mouths of some bays along the coast, the clinoform stretches in the shape of a fan and its thickness is evidently larger than that of the surrounding sediments. This clinoform came into being in the early Holocene(about 11.2 cal kyr BP) and can be divided into the lower and upper depositional units(DU 2 and DU 1, respectively). The unit DU 2, being usually less than 3 m in thickness and formed under a low sedimentation rate, is located between the bottom boundary and the Holocene maximum flooding surface(MFS), and represents the sediment of a post-glacial transgressive systems tract; whereas the unit DU 1, the main body of the clinoform, sits on the MFS, belonging to the sediment of a highstand systems tract from middle Holocene(about 7–6 cal kyr BP) to the present. The provenance of the clinoform differs from that of the typical sediments of the Yellow River and can be considered as the results of the joint contribution from both the Yellow River and the proximal coastal sediments of the Shandong Peninsula, as evidenced by the sediment geochemistry of the core. As is controlled mainly by coactions of multiple factors such as the Holocene sea-level changes, sediment supplies and coastal dynamic conditions, the development of the clinoform is genetically related with the synchronous clinoform or subaqueous deltas around the northeastern Shandong Peninsula and in the northern South Yellow Sea in the spatial distribution and sediment provenance, as previously reported, with all of them being formed from the initial stage of the Holocene up to the present.  相似文献   

20.
Valuable geological and environmental information can be obtained from the 200 m thick lacustrine sediments in the Diexi lake(an ancient landslide-dammed lake) of the Minjiang River. The shaking table test method was employed to study the disturbance phenomena which occurred in the Diexi lake sediments. The results show that the disturbance phenomena were caused by liquefaction-induced flows in the unconsolidated lacustrine sediments, due to triggering by earthquakes. The deformations only occurred in unconsolidated sediment layers and not in consolidated layers. This means that a consolidated layer cannot be liquefied and disturbed again by an earthquake for a second time. The disturbance on one layer corresponds to only one earthquake. The temporal occurrence of earthquakes could be determined by disturbance layers generated at different ages. In total, 10 disturbed layers were found in the lacustrine sediments of the Diexi lake. The experiments showed that there were more than 10 earthquakes between 30 ka B.P. and 15 ka B.P. in the Diexi lake area based on the dating of the disturbed sediment layers.  相似文献   

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