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1.
Proteins of the DYRK (dual-specificity tyrosine-phosphorylation-regulated kinase) family are characterized by the presence of a conserved kinase domain and N-terminal DH box. DYRK2 is involved in regulating key developmental and cellular processes, such as neurogenesis, cell proliferation, cytokinesis, and cellular differentiation. Herein, we report that the ortholog of DYRK2 found in zebrafish shares about 70% identity with that of human, mouse, and chick. RT-PCR showed that DYRK2 is expressed maternally and zygotically. In-situ hybridization results show that DYRK2 is expressed in somite cells that will develop into muscles. Our results provide preliminary evidence for investigating the in-vivo function of DYRK2 in zebrafish muscle development.  相似文献   

2.
提出了实现高阶时域有限差分法FDTD(2M,2N)的一种方法,把对时间的高阶差分转化为对空间的差分,相对于FDTD(2,2N),并不增加存储量。数值实验证明了这一方法的有效性。  相似文献   

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1 Introduction6Alkaline- earth sulfides have been found to beexcellent hosts.. for versatile phosphors such as thecathodo-, photo -, thermo - and electro - luminescent materials (N. Ruelle, M. Pham Thi and C.FOuassier, 1992; S. H. Pawar, 1974). HOwever,no…  相似文献   

4.
随机序列在密码学中有着广泛的应用,其产生方法已经成为密码学的重要研究课题。首先介绍了细胞自动机的基本概念,并详细介绍了2-by-n CA的规则及最大周期CA。最后基于2-by-n CA的特点提出了二维梯形伪随机序列发生方法。计算机模拟表明,基于2-by-n CA的二维梯形伪随机序列发生器具有结构简单、高速、周期长的特点,产生的伪随机序列统计性能好。  相似文献   

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空间复杂性与地理元胞自动机模拟研究   总被引:23,自引:1,他引:22  
面对具有非线性、非平衡、自相似、自组织等复杂性特征的空间复杂现象,基于传统的牛顿力学和微分方程数学的地理模型显得力不从心,非线性科学作为解决地理复杂现象的有效工具成为地理学研究的前沿.基于对地理复杂性的分析、作者提出了一种基于元胞自动机(Cellular Automata)的地理模型框架,并在地理信息系统集成的基础上,对美国安阿伯波市的城市增长与扩散进行了生动地模拟和预测.  相似文献   

8.
在最新的能接收到北斗三号新信号B2a及B2(B2a+b)的MGEX测站数据基础上,分析BDS-3信号的载噪比、多路径及卫星的三维位置精度因子(PDOP)。结果表明,与GPS相比,BDS的载噪比B2(B2a+b)>B2a≈L5>B3≈B2b>B1≈L1>L2,多路径误差B2(B2a+b)相似文献   

9.
Viral entry into the host is the earliest stage of infection in the viral life cycle in which attachment proteins play a key role. VP31 (WSV340/WSSV396), an envelope protein of white spot syndrome virus (WSSV), contains an Arg-Gly-Asp (RGD) peptide domain known as a cellular attachment site. At present, the process of VP31 interacting with shrimp host cells has not been explored. Therefore, the VP31 gene was cloned into pET30a (+), expressed in Escherichia coli strain BL21 and purified with immobilized metal ion affinity chromatography. Four gill cellular proteins of shrimp (Fenneropenaeus chinensis) were pulled down by an affinity column coupled with recombinant VP31 (rVP31), and the amino acid sequences were identified with MALDI-TOF/TOF mass spectrometry. Hemocyanin, beta-actin, arginine kinase (AK), and an unknown protein were suggested as the putative VP31 receptor proteins. SDS-PAGE showed that AK is the predominant binding protein of VP31. An i n vitro binding activity experiment indicated that recombinant AK’s (rAK) binding activity with rVP31 is comparable to that with the same amount of WSSV. These results suggested that AK, as a member of the phosphagen kinase family, plays a role in WSSV infection. This is the first evidence showing that AK is a binding protein of VP31. Further studies on this topic will elucidate WSSV infection mechanism in the future.  相似文献   

10.
Members of the Dna J family are proteins that play a pivotal role in various cellular processes, such as protein folding, protein transport and cellular responses to stress. In the present study, we identified and characterized the full-length Dna J c DNA sequence from expressed sequence tags of Pyropia yezoensis( Py Dna J) via rapid identification of c DNA ends. This c DNA encoded a protein of 429 amino acids, which shared high sequence similarity with other identified Dna J proteins, such as a heat shock protein 40/Dna J from Pyropia haitanensis. The relative m RNA expression level of Py Dna J was investigated using real-time PCR to determine its specific expression during the algal life cycle and during desiccation. The relative m RNA expression level in sporophytes was higher than that in gametophytes and significantly increased during the whole desiccation process. These results indicate that Py Dna J is an authentic member of the Dna J family in plants and red algae and might play a pivotal role in mitigating damage to P. yezoensis during desiccation.  相似文献   

11.
N-(2-hydroxy-1-naphthaldene)-4-aminoantipyrine has been synthesized. The structure is determined by X-ray diffraction method and elemental analysis. The crystal system belongs to orthorhombic space group P(2)2(1)2(1). The geometry has been ob-tained from the density functional theory (DFT) method and the B3LYP method employing the 6-31G* basis sets. The calculated results propose that the latter is close to the experimental data. The structural parameters from the theory are close to those of the crystal and the calculated total energy of coordination is -31677.172 eV. The energy of HOMO and LUMO and the energy gap are 5.179 eV, -1.603 eV and 3.577 eV, respectively.  相似文献   

12.
N-(2-hydroxy-1-naphthaldene)-4-aminoantipyrine has been synthesized. The structure is determined by X-ray diffraction method and elemental analysis. The crystal system belongs to orthorhombic space group P(2)2(1)2(1). The geometry has been ob-tained from the density functional theory (DFT) method and the B3LYP method employing the 6-31G* basis sets. The calculated results propose that the latter is close to the experimental data. The structural parameters from the theory are close to those of the crystal and the calculated total energy of coordination is -31677.172 eV. The energy of HOMO and LUMO and the energy gap are 5.179 eV, -1.603 eV and 3.577 eV, respectively.  相似文献   

13.
This study deals with the toxic effects of Zn2+, Cu2+, Cd2+ and NH3 on Chinese prawn (Penaeus orientalis). The median lethal concentrations of the toxic matters on Chinese prawn were determined. Cd2+>Cu2+>Zn2+>NH3-Nm is the toxic order of these chemicals to Chinese prawn. The toxic order of concentrated Zn2+ and Cd2+ in tissues and organs of Chinese prawn is in the order gill > shell + appendage>viscera > muscle..  相似文献   

14.
Scrippsiella trochoidea (Stein) Loeblich III was grown in a nitrogen-or phosphorus-limited batch culture system in laboratory. Growth rates and cellular Chl-a were measured as functions of nitrate and phosphate concentrations. Growth rates were hyperbolic with both nitrate and phosphate concentration and fit the Monod equation. The minimum cell quota of nitrogen and phosphorus and then the optimum N∶P ratio ofS. trochoidea were estimated in this study. Measurement of phosphate concentration in Jiaozhou Bay suggest that phosphorus is the limiting factor ofS. trochoidea growth. Contribution No. 3679 from the Institute of Oceanology, Chinese Academy of Sciences. Project 39790110 supported by NSFC, the study aslo supported by the National Climbing Project B (PDBG-7-2) and partly supported by an MF grant from Hong Kong University of Science and Technology.  相似文献   

15.
Phaeodactylum tricornutum is one of the important marine diatoms for oceanic primary production. Its reproduction has profound significance in the life cycle; however, the nuclear behavior during its sexual reproduction was not clear. In this study, we observed the nuclear transition and determined its correlation with cell conjunction. It was found that two cells jointed at their apices first and swung and aligned each other immediately, and nucleus from one cell was able to transfer into another one during cell conjugation. The cell pairs conjugated for nuclear transition were different from those formed in mitosis in hypovalve thickness and cellular arrangement. Our findings proved the existence of sexual reproduction in P. tricornutum.  相似文献   

16.
The androgenic gland(AG) is an important endocrine gland for male reproductive function in crustaceans. In the present study, we investigated the histological characteristics of the androgenic gland of peppermint shrimp, Lysmata wurdemanni. The peppermint shrimp matures as male first, then some individuals may become euhermaphrodite after several moltings(transitional phase). Euhermaphrodite-phase shrimp acts as male at intermolts. However, it can be fertilized as a female immediately after molting. Considering the male reproductive function acts in its lifespan except for at larval stages, and female reproductive system starts to develop at transitional phase, we hypothesized that AG activity might be reduced to allow and promote vitellogenesis onset in early transitional phase and the following euhermaphrodite phase. So AG cell structure might be different in three phases in L. wurdemanni. The results showed that AG exists in the male in transitional and euhermaphrodite phases. The gland cell clusters surrounding the ejaculatory ducts locate at the roots of the fifth pereopods. The nucleus diameters are similar in the three phases while the nucleus-to-cell ratio is the lowest in euhermaphrodite phase. Our results indicated that for the individuals that will become euhermaphrodite, the cellular structure of AG changes since transitional phase. Male reproductive function which is still available in euhermaphrodite-phase shrimp should be due to the existence of the gland.  相似文献   

17.
We studied the effects of mercury (Hg2+) on antioxidant and digestive enzyme activities in terms of LC50 value and on hepatopancreas histostructures of juvenile Chinese mitten crabs Eriocheir sinensis in 40-day exposure to various concentrations of Hg2+ (0, 0.01, 0.05, 0.10, 0.20, and 0.30 mg/L). The results show that the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) significantly increased in the concentrations of 0.01 and 0.05 mg/L, while that of enzyme decreased in 0.10, 0.20 and 0.30 mg/L treatments. Meanwhile, Hg2+ disrupted the histostructures of the hepatopancreas, causing decreases in activities of pepsin, tryptase, amylase, and cellulose, which are synthesized in the hepatopancreas. Moreover, as the Hg2+ concentration increased, the survival rate of the crabs decreased, worst at 56.57% in 0.30 mg/L. Therefore, although crabs are able to tolerate low levels of mercury pollution, high levels lead to cellular injury and tissue damage in hepatopancreas, which then loses some of its vital physiological functions such as absorption, storage, and secretion.  相似文献   

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Lead(Pb) and its compounds are common pollutants in industrial wastewaters.To develop appropriate Pb 2+ treatment technologies,aerobic granules(AG) and bacterial alginates(BA) were studied as alternative biosorbents to remove Pb 2+ from aqueous solutions.The biosorption mechanism of AG and BA were further analyzed to determine which functional groups in AG and BA are active in Pb 2+ biosorption.In this paper,the Pb 2+ biosorption behavior of AG and BA was respectively investigated in batch experiments from the perspectives of the initial pH,contact time,and initial Pb 2+ concentration.The results showed that biosorption of Pb 2+ by AG and BA occurred within 60min at the initial Pb 2+ concentrations(0 150 mg L-1).The actual saturated Pb 2+ biosorption capability of AG was 101.97 mg g-1(dry weight of aerobic granular biomass).When the initial pH was 5,the biosorption capability of AG and BA was highest at the initial Pb 2+ concentrations(0 20mg L-1).During the process of Pb 2+ biosorption,K +,Ca 2+,and Mg 2+ were released.The Ion Chromatography(IC) and Fourier Transform Infrared Spectroscopy(FTIR) further highlighted the main role of ion exchange between Ca 2+ and Pb 2+ and sequestration of Pb 2+ with carboxyl(-COO) of AG and BA.This analogical analysis verifies that BA is responsible for biosorption of Pb 2+ by AG.At the same optimal pH,AG cultivated with different carbon source has different Pb 2+ biosorption capacity.The Pb 2+ biosorption by AG with sodium acetate as the sole carbon source is higher than AG with glucose as carbon source.  相似文献   

20.
本文从化学平衡的角度讨论了开放体系中二氧化碳的分压(Pco_2)对CaCO_3溶解量的影响。根据定量计算的结果,CaCO_3的溶解量之所以随着Pco_2的升高而增大,主要是由于以下三方面的原因所造成的:首先,随着Pco_2的升高,水溶液的pH将会降低,从而使水溶液中CO_3~(2-)的含量减小,因此溶液中CaCO_3的浓度积将会减小,结果便导致了CaCo_3的溶解;其次,随着Pco_2的升高,水溶液中HCO_3~-的含量将会增大,这样便使得以CaHCO_3~+的形式结的Ca~(2+)的含量增多,这也导致了CaCO_3溶解量增的大;第三,CaCO_3解量增大的结果,使溶液的离子强度增加,这又导致了各离子活度系数的减小,从而也促进了CaCO_3的溶解。文章最后还讨论了应该怎样去定性地解释Pco_2对CaCO_3溶解量的影响,并认为使用反应; CaCO_3+H_2O + C_2OCa~(2+)+2HCO_3~-来定性地解释Pco_2对CaCO_3溶解量的影响,存在有以下两个方面的问题:第一,该反应没有反映出Pco_2升高或降低过程中水溶液中最重要的因素之一的〔H~+〕的变化;第二,对于与CaCO_3相平衡的水溶液,当Pco_2升高时,用该反应来解释会导致矛盾的结果。  相似文献   

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