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1.
Black rockfish(Sebastes schlegeli) is an important species for culture; however, its reproductive characteristics have not been fully documented. In this study, we investigated the morphology and developmental process of germ cells in this ovoviviparous rockfish in reproductive season(October 2011–November 2012) with histological methods. We found that the gonad of mature fish showed notable seasonal changes in developmental characteristics and morphological structure. The sperm cells matured during a period lasting from October to December, significantly earlier than the oocytes did. A large number of spermatozoa and other cells occurred in testis at different developmental stages. Vitellogenesis in oocytes began in October, and gestation appeared in April next year. Spermatophores were discovered for the first time in Sebastes, which assembled in testis, main sperm duct, oviduct and genital tract, as well as ovarian cavity in October and April. These organs may serve either as production or hiding places for spermatophores and spermatozoa which were stored and transported in form of spermatophores. Testicular degeneration started from the distal part of testis in April, with spermatophores assembled in degenerating testis and waiting for transportation. The copulation probably lasted for a long period, during which the spermatozoa were discharged in batches as spermatophores. These spermatophores were coated with sticky materials secreted from the interstitial areas of testis and the main sperm duct, then transported into ovary.  相似文献   

2.
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3.
The predictability of certain environmental factors that affect the life cycle of the seabob shrimp Xiphopenaeus kroyeri(Heller,1862) was evaluated in a study of its reproductive biology in an area adjacent to Babitonga Bay,State of Santa Catarina,Brazil. Monthly sampling was conducted from July 2010 through June 2011 at depths of 5,8,11,14,and 17 m.76 004 individuals were obtained,with a pronounced peak in absolute abundance in austral autumn(34 208),coinciding with the annual closed season from March to May. Grain size composition of the sediment showed the closest relationship to the distribution of individuals(multiple linear regression,P 0.05),related to their burying habit. The observed correlations between the abundance of reproductive males(bearing spermatophores) and females with spent gonads(cross-correlation,P 0.05),and between reproductive males and reproductive females(with a 1-month lag) suggest that the peak of reproductive males preceded the peak offemale ones. This result agrees with the pattern expected for females,which copulate in post-ecdysis(spent gonads). Spawning seemed to take place at greater depths,as evidenced by the concentration of reproductive females in these areas. The reproductive activities observed here confirm that this species follows a tropical/subtropical reproductive pattern,spawning continuously throughout the year,with the highest peaks in spring and autumn. The data indicate that the juvenile recruitment period observed in August-September resulted from the reproductive output noted in April–May. Additionally,the reproductive period recorded in November led to the juvenile peak observed in March–May.  相似文献   

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5.
The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis.A new Sertoli cell line(POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages.Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P.olivaceus.Cells were optimally maintained at 25℃ in DMEM/F12 medium supplemented with fetal bovine serum,basic fibroblast growth factor,and epidermal growth factor.The growth curve of POSC showed a typical "S" shape.Chromosome analysis revealed that the cell line possessed the normal P.olivaceus diploid karyotype of 2n=48t.POSC expressed dmrt1 but not vasa,which was detected using RT-PCR and sequencing.Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL.Therefore,POSC appeared to consist of testicular Sertoli cells.Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid,with the transfection efficiency reaching 10%.This research not only offers an ideal model for further gene expression and regulation studies on P.olivaceus,but also serves as valuable material in studying fish spermatogenesis,Sertoli cell-germ cell interactions,and the mechanism of growth and development of testis.  相似文献   

6.
Histological characteristics of gametogenesis of two kinds of scallops, gonochoric Chinese scallop, Chlamys farreri and hermaphroditic bay scallop, Argopecten irradians were investigated in this study. Spermatogenesis in C. farreri has different developmental stages: spermatogonia, primary spermatocyte, second spermatocyte, spermatid and spermatozoon. A large number of same developmental stage spermatic cells converge at a definite area of the testis. Premeiotic, previtellogenic and vitellogenic oocytes can be found during oogenesis in C. farreri, where oocyte distribution is obviously irregular. The A. irradians gonad consists of two different parts in one individual: one part functions as testis, the other as ovary. Between these two parts is a special appearance area, where a large number of spermatic cells are bound with two layers of acellular substance with many oocytes in it.  相似文献   

7.
Drag forces acting on Schlegel‘s black rockfish Sebastes schlegeli were studied. A new drag force transducer was designed and used to measure the water drag on Schlegel‘s black rockfish in a vertical recirculating flume tank. Fourteen individuals were investigated, yielding two mean drag coefficients referred to the cross-sectional area and volume^2/3 respectively at water velocities ranging from 0.3 to 1.0 ms^-1 The drag coefficients can be used for estimating the drag forces acting on Sebastes schlegeli in water.  相似文献   

8.
After decades of low year classes,the stock of Japanese sardine(Sardinops melanostictus)has begun to recover since the mid-2000s.The hatch dates and otolith growth rates of age-0 juvenile sardine,which were collected in the subarctic Oyashio waters in autumn 2018,were determined from an otolith microstructure analysis.The sardines were hatched from late January to late April,while mostly in February and March.The otolith growth rate increased continuously up to 60 d after hatching and thereafter de-creased.The revealed growth rate in a crucial growth period is faster than that reported for juvenile sardines collected in the 1990s,which is coincided with the recent recovery trend of the sardine stock.Two groups with different hatch dates,growth histories,and migration routes were identified using unsupervised random forest clustering analysis.They were considered inshore and offshore migration individuals in accordance with recent researches.In the offshore group,a high proportion of sardine juveniles hatched late and grew faster in the Kuroshio-Oyashio transitional waters,a finding consistent with the hypothesis of growth-rate-dependent re-cruitment.This finding on the population composition and growth rate of juvenile sardine in the Oyashio waters can be a basis for an improved prediction of their survival and provides us with valuable information on the recruitment processes of this stock during the period of stock recovery.  相似文献   

9.
Lymphocystis nodules occurring in the cultured sting fish Sebastes schlegeli were observed under light and electron microscope. Lymphocystis disease virus (LCDV) in the tissues of diseased fish was detected with indirect immunofluorescence test (IFAT). Results showed that lymphocystis cells had overly irregular nuclei, basophilic intracytoplasmic inclusion bodies with virions budding from the surface, and hyaline capsules outside the cell membrane. Numerous virus particles about 200 nm in diameter scat- tered in the cytoplasm, electron-dense particles 70-80 nm in diameter filled in perinuclear cisterna, and membrane-enveloped parti- cles with electron-dense core of 70-80 nm appeared around cellular nucleus. IFAT using monoclonal antibody against LCDV from Paralichthys olivaceus revealed that specific green fluorescence was present in the cytoplasm of lymphocystis cells, epithelium of stomach, gill lamellae, and muscular fibers under epidermis of S. schlegeli, just as that in the cytoplasm of lymphocystis cells of P. olivaceus, suggesting the presence of LCDV in these tissues.  相似文献   

10.
Hazarganji Chiltan National Park in Balochistan, Pakistan was established in 1980 and the protected area was further extended in 1998. Large area of this mountain is still open for unmanaged human disturbances such as collection of wood for fuel purpose and livestock grazing. Removal of vegetation of rangelands has a significant negative impact on soil organic matter(SOM). This research evaluates litter decomposition in three sites of Hazarganji Chiltan mountain with varying history of human disturbances(unprotected site, young protected site and old protected site). Twigs of Pistacia khinjuk with approximately equal weight and length were placed in litter bags of mesh size 2 mm and were buried in 0-5 cm depth in three sites in January. Half of the twigs of each site received rain simulation in April, August, October, November and January while the other half of the twigs were subjected under natural conditions for 15 months. Twigs from each plot of each treatment of each site were collected from soil after every rain simulation in the previous month of experiment and were processed for weight loss assessment. Results showed that weight loss of twigs by decomposition was significantly higher in the soil of unprotected site as compared to other two sites and there was no difference between rain simulation and control treatments except that loss of weight of twigs of unprotected site was higher under control than rain simulation condition. To confirm that SOM was the major controlling factor for the decomposition of litter decay, soils of each site were collected and burned to remove SOM;thereafter, burned soil samples were mixed with homogenous powder of oven-dried native plants, incubated for 6 months and were provided with dissolved organic matter of the soils of each site. Results showed that there was no difference in the decomposition of litter between soils under controlled laboratory condition, which confirmed that SOM was a major controlling factor for the litter decay in soil under field conditions. The pyrosequencing analysis of the DNA of soils collected from three sites revealed the presence of bacterial species Thermovum composti.  相似文献   

11.
The generalized linear model （GLM） and generalized additive model （GAM） were applied to the standardization of catch per unit effort （CPUE） for Chilean jack mackerel from Chinese factory trawl fishing fleets in the Southeast Pacific Ocean from 2001 to 2010 by removing the operational, environmental, spatial and temporal impacts. A total of 9 factors were selected to build the GLM and GAM, i.e., Year, Month, Vessel, La Nifia and E1 Nifio events （ELE）, Latitude, Longitude, Sea surface temperature （SST）, SST anomaly （SSTA）, Nino3.4 index and an interaction term between Longitude and Latitude. The first 5 factors were significant components in the GLM, which in combination explained 27.34% of the total variance in nominal CPUE. In the stepwise GAM, all factors explained 30.78% of the total variance, with Month, Year and Vessel as the main factors influencing CPUE. The higher CPUE occurred during the period April to July at a SST range of 12-15℃ and a SSTA range of 0.2-1.0℃. The CPUE was significantly higher in normal years compared with that in La Nifia and E1 Nifio years. The abundance of Chilean jack mackerel declined during 2001 and 2010, with an increase in 2007. This work provided the relative abundance index of Chilean jack mackerel for stock as- sessment by standardizing catch and effort data of Chinese trawl fisheries and examined the influence of temporal, spatial, environ- mental and fisheries operational factors on Chilean jack mackerel CPUE.  相似文献   

12.
Seagrass habitats are structurally complex ecosystems, which support high productivity and biodiversity. In temperate systems the density of seagrass may change seasonally, and this may influence the associated fish and invertebrate community. Little is known about the role of seagrass beds as possible nursery areas for fish and invertebrates in China. To study the functioning of a seagrass habitat in northern China, demersal fish and invertebrates were collected monthly using traps, from February 2009 to January 2010. The density, leaf length and biomass of the dominant seagrass Zostera marina and water temperature were also measured. The study was conducted in a Seagrass Natural Reserve(SNR) on the eastern coast of the Shandong Peninsula, China. A total of 22 fish species and five invertebrate species were recorded over the year. The dominant fish species were Synechogobius ommaturus, Sebastes schlegelii, Pholis fangi, Pagrus major and Hexagrammos otakii and these species accounted for 87% of the total number of fish. The dominant invertebrate species were Charybdis japonica and Octopus variabilis and these accounted for 98% of the total abundance of invertebrates. There was high temporal variation in species composition and abundance. The peak number of fish species occurred in August–October 2009, while the number of individual fish and biomass was highest during November 2009. Invertebrate numbers and biomass was highest in March, April, July and September 2009. Temporal changes in species abundance of fishes and invertebrates corresponded with changes in the shoot density and leaf length of the seagrass, Zostera marina.  相似文献   

13.
The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione reductase(Gr), and lipid peroxidation(measured via malondialdehyde(MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol(PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation(only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could signifi cantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had signifi cant eff ects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.  相似文献   

14.
Neuronal nitric oxide synthase(nNOS) was the producer of nitric oxide(NO) which played important gas messenger molecules in biological process. It also can take effect as immune regulation molecule in organism. Black rockfish(Sebastes schlegelii) is an important economic fish which were widely farmed in East Asia countries. Meanwhile, the pathogenic bacteria such as the Edwardsiella tarda and Vibrio anguillarum in seawater always brought serious obstacles to their healthy growth. In order to exp...  相似文献   

15.
Observations on gonad development of 84 species of marine fishes in Jiaozhou Bay waters showed the breeding periods of more than 50 of these species are mostly from April to August, with peaks between May to June. A great many of the species spawn in the warm season, only a few species in the cold season. The number of spawning species increases with the rise and decreases with the fall in water temperature. The absolute fecundity of 50 teleost species, which usually increases with body length and weight, was estimated to range from about 300 to 9,000,000 eggs. The reproductive patterns of fishes in Jiaozhou Bay waters are discussed.  相似文献   

16.
Introduced species may outcompete or hybridize with native species, resulting in the loss of native biodiversity or even alteration of ecosystem processes. In this study, we reported an alien distromatic Ulva species, which was found in an embayment（Holly Pond） connected with Long Island Sound, USA. The morphological and anatomical observations in combination with molecular data were used for its identification to species. Anatomy of collected specimens showed that the cell shape in rhizoidal and basal regions was round and the marginal teeth along the basal and median region were not found. These characteristics were primarily identical to the diagnostic characteristics of Ulva laetevirens Areschoug（Chlorophyta）. The plastid-encoding tufA and nucleus-encoding ITS1 were used for its molecular identification. Phylogenetic analysis for the tufA gene placed the specimens from Holly Pond in a well-supported clade along with published sequences of U. laetevirens identified early without any sequence divergence. In ITS tree, the sample also formed well-supported clades with the sequences of U. laetevirens with an estimated sequence divergence among the taxa in these clades as low as 1%. These findings confirmed the morpho-anatomical conclusion. Native to Australia, this species was reported in several countries along the Mediterranean coast after the late of 1990 s. This is the first time that U. laetevirens is found in the northeast coast of United States and the second record for Atlantic North America.  相似文献   

17.
Phytoplankton and environmental variables were measured monthly from July 2009 to August 2011 in the Maixi River from the estuary to Baihua Reservoir in the Maotiao River catchment,southwestern China,to understand phytoplankton community structure and environmental factors.The relationship between phytoplankton community structure and environmental factors including hydrological,meteorological,physical,and chemical variables were explored using multivariate analysis.A total of 81 taxa of phytoplankton were identified,which were mainly composed of chlorophyta,bacillariophyta,and cyanobacteria.The phytoplankton community was dominated by Pseudanabaena limnetica during summer and fall and by Cyclotella meneghiniana during winter and spring.The abundance of phytoplankton ranged from 0.24×104 cells/L to 33.45×106 cells/L,with the minimum occurring during February 2010 and the maximum during July 2009.The phytoplankton community was dominated mainly by cyanobacteria from April to September,and by bacillariophyta and pyrrophyta from October to March.Canonical correspondence analysis showed that temperature,pH values,and orthophosphate were the most important driving factors regulating the composition and dynamics of the phytoplankton community in the estuary.Cyanobacteria and euglenophyta abundance and biomass were affected mainly by temperature and pH values,while most chlorophyta and bacillariophyta were influenced by the concentrations of nutrients.  相似文献   

18.
A 12-month program of monitoring potentially toxic microalgae(that produce lipophilic shellfi sh toxins; LSTs) and their toxins in bivalves was conducted from April 2006 to March 2007 in the Nanji Islands, East China Sea. Two Dinophysis species, D. caudata and D. acuminata, were identifi ed, and D. caudat a was found to be the dominant species. D. caudata was detected in water samples between April and June 2006, and between February and March 2007. It reached its highest abundances in May, with a mean abundance of 1.38×10 2 cells/L in surface water and 1.25×10 2 cells/L in bottom water(10 m deep). The temporal distribution of D. caudata was associated with the occurrence of LSTs in bivalve samples, which mostly occurred at the same time as D. caudata blooms, between April and July 2006. All of the cultured bivalves sampled between April and June were contaminated with LSTs, with an average toxicity of 85 μg okadaic acid(OA) eq./100 g meat, which was four times higher than the Chinese regulatory limit(20 μg OA eq./100 g meat). Ten out of fi fteen wild samples(66.7%) collected during the same period were positive for LSTs, and contained an average LST toxicity of 45 μg OA eq./100 g meat(more than twice the regulatory value). Cultured Patinopecten yessoensis collected on 15 May 2006 had the highest toxicity, 320 μg OA eq./100 g meat, and relatively high toxicities(80 to 160 μg OA eq./100 g meat) were found in bivalves until the end of July.  相似文献   

19.
Based on the time series data from the Aral hydrological station for the period of 1958-2005, the paper reveals the long-term trend and fractal of the annual runoff process in the mainstream of the Tarim River by using the wavelet analysis method and the fractal theory. The main conclusions are as follows： 1） From a large time scale point of view, i.e. the time scale of 16 （24） years, the annual runoff basically shows a slightly decreasing trend as a whole from 1958 to 2005. If the time scale is reduced to 8 （23） or 4 （22） years, the annual runoff still displays the basic trend as the large time scale, but it has fluctuated more obviously during the period. 2） The correlation dimension for the annual runoff process is 3.4307, non-integral, which indicates that the process has both fractal and chaotic characteristics. The correlation dimension is above 3, which means that at least four independent variables are needed to describe the dynamics of the annual runoff process. 3） The Hurst exponent for the first period （1958-1973） is 0.5036, which equals 0.5 approximately and indicates that the annual runoff process is in chaos. The Hurst exponents for the second （1974-1989） and third （1990-2005） periods are both greater than 0.50, which indicate that the annual runoff process showed a long-enduring characteristic in the two periods. The Hurst exponent for the period from 1990 to 2005 indicates that the annual runoff will show a slightly increasing trend in the 16 years after 2005.  相似文献   

20.
The small yellow croaker Larimichthys polyactis is an economically important marine fish in Northeast Asia. Currently,its natural resources are threatened by overfishing and environmental pollution. Therefore, research on the reproductive system of the fish is crucial. Here, we studied the testis development and ultrastructural features of spermatogenesis in cultured L. polyactis using anatomical, histological, and ultrastructural techniques. A pair of testes, consisting of a central sperm duct ...  相似文献   

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