共查询到18条相似文献，搜索用时 312 毫秒
1.
采用聚丙烯酰胺梯度凝胶垂直平板电泳对湛江地区的光裸星虫(Sipunculus nudus)3种组织(肠组织、体腔液和体壁肌肉)12种同工酶(SOD、EST、MDH、LDH、ALP、AMY、ME、ACP、G6PDH、SDH、ADH、GPI)进行检测分析。结果表明:检测的12种同工酶中的7种酶(SOD、EST、MDH、LDH、ALP、AMY、ME)具有明显的组织特异性;GPI在组织中表达不稳定,未检测到其他4种同工酶(ACP、G6PDH、SDH、ADH)的活性;光裸星虫酶谱带数目少,酶谱构型单一。并说明了对其种群内遗传生化特性进行系统研究的必要性。 相似文献
2.
企鹅珍珠贝同工酶表达多态性的初步研究 总被引：4，自引：0，他引：4
采用垂直板状聚丙烯酰胺梯度凝胶电泳,对企鹅珍珠贝鳃组织的酯酶(EST)、葡萄糖-6磷酸脱氢酶(G6PD)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)和超氧化物歧化酶(SOD)5种同工酶进行了检测,并对酶谱特征进行了生化遗传分析.酶谱表型显示企鹅珍珠贝的同工酶呈现多态现象,同工酶表达上的多态性表明企鹅珍珠贝具有较丰富的遗传多样性. 相似文献
3.
采用垂直板状聚丙烯酰胺梯度凝胶电泳，对企鹅珍珠贝鳃组织的酯酶(EST)、葡萄糖-6磷酸脱氢酶(G6PD)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)和超氧化物歧化酶(SOD)5种同工酶进行了检测，并对酶谱特征进行了生化遗传分析。酶谱表型显示企鹅珍珠贝的同工酶呈现多态现象，同工酶表达上的多态性表明企鹅珍珠贝具有较丰富的遗传多样性。 相似文献
4.
军曹鱼不同组织5种同工酶的研究 总被引：2，自引：0，他引：2
采用聚丙烯酰胺垂直凝胶电泳法对军曹鱼的几种组织 (脑、心肌、肾、肌肉、性腺、肝、血液、胰、脾 )的 5种同工酶 (LDH、MDH、ME、POD和EST)进行了分析 ,结果表明 :5种同工酶在军曹鱼中都具有明显的组织特异性 ,这些特异性与其组织特定生理功能相关。与其他硬骨鱼类相比 ,军曹鱼的酶谱简单得多 ,这可能与其种属多样性相关。 相似文献
5.
取二色裂江珧 (Pinna bicolor)的消化盲囊、肾脏、后闭壳肌肌肉、外套膜和鳃等 5种组织 ,用垂直板聚丙烯酰胺凝胶电泳对酯酶 (EST)和过氧化物歧化酶 (SOD)同工酶在上述组织中的表达和分布进行了比较研究 ,并对酶谱表型及位点表达进行了分析。结果表明 ,二色裂江珧组织内的 EST和 SOD存在不同程度的组织特异性 ,EST共检测出 15条酶带 ,SOD检测出 8条酶带 ,且两种同工酶在消化盲囊中活性最高。推测 SOD由 2个座位编码 ,可分为 s- SOD二聚体和 m- SOD四聚体 相似文献
6.
采用垂直板聚丙烯酰胺凝胶电泳技术,对月鳢早期(从未受精卵至卵黄吸尽期)8个发育阶段的LDH、MDH、ADH、EST同工酶进行研究。结果表明:月鳢的早期发育阶段ADH酶谱相对稳定,均有两条谱带;LDH、MDH和EST三种同工酶的酶谱在神经胚期前均无明显变化,此后随发育进程逐渐复杂。 相似文献
7.
黑斑蛙(Rana nigromaculata)早期发育过程中乳酸脱氢酶同工酶 总被引：3，自引：1，他引：2
用垂直平板聚丙烯酸胺凝胶电泳技术对黑斑蛙早期胚胎发育过程中乳酸脱氢酶（LDH）同工酶进行研究。结果表明,黑斑蛙胚胎各个不同发育时期LDH同工酶谱有很大差异：LDH1在整个胚胎发育过程中均处于活化状态,LDH2、LDH4在原肠中期开始出现,LDH3在尾芽期开始出现。从尾芽期到尾血循环期只出现LDH－1、－2、－3和-4四条酶带,但不同时期各种同工酶的表达活性不同。 相似文献
8.
月鳢个体发育早期4种同工酶的初步研究 总被引：2，自引：0，他引：2
采用垂直板聚丙烯酰胺凝胶电泳技术，对月鳢早期(从未受精卵至卵黄吸尽期)8个发育阶段的LDH、MDH、ADH、EST同工酶进行研究。结果表明：月鳢的早期发育阶段ADH酶谱相对稳定，均有两条谱带；LDH、MDH和EST三种同工酶的酶谱在神经胚期前均无明显变化，此后随发育进程逐渐复杂。 相似文献
9.
二色裂江珧EST和SOD同工酶组织特异性研究 总被引：8，自引：0，他引：8
取二色裂江珧（Ｐｉｎｎａ ｂｉｃｏｌｏｒ）的消化盲囊、肾脏、后闭壳肌肌肉、外套膜和鳃等５种组织,用垂直板聚丙烯酰胺凝胶电泳对酯酶（ＥＳＴ）的过氧化物歧化酶（ＳＯＤ）同工酶在上述组织中的表达和分布进行了比较研究,并对酶谱表型及位点表达进行了分析。结果表明,二色裂江珧组织内的ＥＳＴ和ＳＯＤ存在不同程度的组织特异性,ＥＳＴ共检测出１５条酶带,ＳＯＤ检测出８条酶带,且两种同工酶在消化盲囊中活性最高。推测Ｓ 相似文献
10.
采用聚丙烯酰胺垂直板梯度凝胶电泳技术,对管角螺7种组织(外套膜、肌肉、肝脏、心脏、生殖腺和缠卵腺)的5种同工酶(EST、SOD、ME、MDH、LDH)进行了分析,结果表明:5种同工酶存在不同程度的组织特异性,SOD、EST、LDH在肝脏组织中有较高活性,而MDH、ME在心脏组织中活性较高。 相似文献
11.
ZHENG Xiaodong ) * ZHAO Jianmin ) XIAO Shu ) WANG Rucai ) WANG Shidang ) ZHOU Weiwu ) ) The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao P..R.China ) Wendeng Fishery Technical Extension Station Weihai P..R.China 《中国海洋大学学报(英文版)》2004,3(1)
1 Introduction ThegoldencuttlefishSepiaesculenta,aneriticanddemersalspecies,livesinthedepthof 1 0 - 1 0 0meters,andsometimesinseabottomsand .Itiswidelydistrib utedfromcentralHonshutoVietnamandthePhilip pines .Thegoldencuttlefishisalsoanimportantobjectof… 相似文献
12.
Zheng Xiaodong Zhao Jianmin Xiao Shu Wang Rucai Wang Shidang Zhou Weiwu 《中国海洋大学学报(英文版)》2004,3(1):48-52
Thirty-nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH, MDH, MEP,
IDHP, PGDH, GRS, NP, AAT, CK, AK, EST, ALP, ACP, FBP, MPI, GPI and PGM) show strong activities and good convergence in zymogram.
They are proved to be suitable genetic markers in Sepia esculenta. Among the tissues used, mantle muscle is the best for electrophoretic analysis of isozymes. Eye and liver are fairly good
for some special enzymes, such as LDH, EST, MPI, etc. Twenty-six loci are detected. The proportion of polymorphic loci is 0.115 in the Qingdao sample and 0.153 in the Rizhao
sample (P<0.99). The mean values of the observed and expected heterozygosity per locus of Qingdao sample are 0.016 and 0.017, while
those of the Rizhao sample are 0.023 and 0.025 respectively. 相似文献
13.
用聚丙烯酰胺垂直板不连续凝胶电泳法对湛江海域褐菖鲉(Sebastiscus marmoratus)心、肝、肾、性腺、肌肉、鳃、眼、脑等8种组织的12种同工酶(ADH、a-AMY、CAT、EST、GDH、LDH、MDH、ME、POD、PPO、SDH、SOD)进行分析。结果表明,12种同工酶均有明显的组织特异性。经筛选,对肝脏和眼的8种同工酶(ADH、EST、LDH、MDH、ME、POD、SDH、SOD)进行群体遗传结构分析,共记录了18个基因位点,其中呈多态性的基因位点有5个(Adh-1、Est-2、m-Pod-1、s-Pod-1、s-Sod-1),多态位点比例(P)为27.78%,平均有效等位基因数(Ae)为1.238 8,平均观测杂合度(Ho)值为0.1401。 相似文献
14.
LIUManhong GAOTianxiang ZHANGXiumei CHENSiqing 《中国海洋大学学报(英文版)》2005,4(1):43-48
To investigate the tissue-specificities of isozymes and the genetic structure of wild spotted halibut ( Verasper variegatus) population, horizontal starch gel electrophoresis was performed on 45 individuals collected in part of the Yellow Sea. The performances of 17 isozymes in 8 kinds of tissues or organs were screened preliminarily in a TC-7.0 buffer system. The results showed that the screened isozymes displayed remarkable tissue-specificities. Finally, 14 enzymes (AAT, ADH, EST, GPI, G3PDH, IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) and 4 kinds of tissues (eye, skeleton muscle, liver and heart) were selected for genetic analysis. Fourteen isozymes are encoded by 20 loci, and 9 of them are poly-morphic. The polymorphic loci are AAT-1*, GPI-2*, G3PDH*, IDHP-1*, LDH*, MPI*, PGM-1*, PGM-2* and SDH*, and the proportion of polymorphic loci is 0.450 0 (P0.99). The mean values of observed and expected heterozygosities are 0.027 8 and 0.026 5, respectively and the average effective number of alleles is 1.067 5. 相似文献
15.
To investigate the tissue-specificities of isozymes and the genetic structure of wild spotted halibut (Verasper variegates) population, horizontal starch gel electrophoresis was performed on 45 individuals collected in part of the Yellow Sea. The
performances of 17 isozymes in 8 kinds of tissues or organs were screened preliminarily in a TC-7.0 buffer system. The results
showed that the screened isozymes displayed remarkable tissue-specificities. Finally, 14 enzymes (AAT, ADH, EST, GPI, G3PDH,
IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) and 4 kinds of tissues (eye, skeleton muscle, liver and heart) were selected
for genetic analysis. Fourteen isozymes are encoded by 20 loci, and 9 of them are polymorphic. The polymorphic loci are AAT-1*.
GPI-2* G3PDH*, IDHP-1*, LDH*, MPI*, PGM-1*. PGM-2* and SDH*, and the proportion of polymorphic loci is 0.4500 (P0.99). The mean values of observed and expected heterozygosities are 0.027 8 and 0.026 5, respectively and the average effective
number of alleles is 1.067 5. 相似文献
16.
《山地科学学报》2019,(12)
China is undergoing a rapid urbanization process, and urbanization will have a direct impact on regional ecosystems and affect regional ecosystem services. Considering the mountainous counties in Southwestern China as the research object, this study reveals the spatial clustering characteristics of four typical ecosystem services(food production, soil conservation, water yield and carbon sequestration)as well as the trade-offs and synergies among ecosystem services in different urbanized areas. At the same time, piecewise linear regression is used to determine the threshold of the influence of urbanization on ecosystem services. The results indicate that: 1) There are spatial autocorrelations among the four typical ecosystem services; with strong clustering characteristics, the positive correlation types are "clustered" locally; and with significant spatial heterogeneity, the negative correlation types are scattered and mainly appear in the highly urbanized area. 2) There are also remarkable differences in the relationship among various ecosystem services in different urbanized areas, and in particular, there are marked trade-offs between food production and carbon sequestration in the moderately urbanized area and the highly urbanized area. However, there are synergies between them in the lowly urbanized area. 3) With an increase in the compounded night light index(CNLI), water yield, carbon sequestration, food production and overall ecosystem services values present an increasing-decreasing trend, the soil conservation function value shows a decreasing-increasing trend.The response of water yield, carbon sequestration,food production, and overall ecosystem services to the compounded night light index(CNLI) has a threshold of 1.2642, 1.4833, 1.3388, 1.5146 and 1.2237,respectively. Based on the detected relationships between urbanization and ecosystem services, this study provides a theoretical reference for the selection of urbanization development models in key ecological functional areas. 相似文献
17.
通过对172 km的高分辨率浅地层剖面与6个钻孔的综合对比,对丁字湾近岸海底沉积物进行细分,自上而下划分为D0,D1,D2,D3共4个反射界面,SU1,SU2,SU3共3个沉积单元,该文总结了每个沉积单元的埋深、分布及特征,按照各个层序形成时的相对海平面位置及其升降变化态势,划分了海侵、低位、高位3个体系域,分别对应滨海相、陆相、滨海相沉积相。查明了丁字湾近岸浅地层结构及沉积特征,为该区的发展规划、海洋工程建设提供地质依据。 相似文献
18.
采用垂直板聚丙烯酰胺凝胶电泳技术,对月鳢十种组织中乳酸脱氢酶（ＬＤＨ）和酯酶（ＥＳＴ）同工酶进行了比较研究,结果表明：月鳢组织内ＬＤＨ和ＥＳＴ具有明显的组织特异性,ＥＳＴ由２个座位编码,ＬＤＨ则由Ｌｄｈ－ａ、Ｌｄｈ－ｂ两个基因编码。 相似文献