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1.
The present study was conducted to investigate the effect of gamma irradiation on white spot syndrome virus(WSSV). White spot syndrome virus is a pathogen of major economic importance in cultured penaeid shrimp industries. White spot disease can cause mortalities reaching 100% within 3–10 days of gross signs appearing. During the period of culture, immunostimulant agents and vaccines may provide potential methods to protect shrimps from opportunistic and pathogenic microrganisms. In this study, firstly, WSSV was isolated from infected shrimp and then multiplied in crayfish. WSSV was purified from the infected crayfish haemolymph by sucrose gradient and confirmed by transmission electron microscopy. In vivo virus titration was performed in shrimp, Penaeus semisulcatus. The LD50 of live virus stock was calculated 10 5.4 /mL. Shrimp post-larvae(1–2 g) were treated with gamma-irradiated(different doses) WSSV(10 0 to 10-4 dilutions) for a period of 10 days. The dose/survival curve for irradiated and un-irradiated WSSV was drawn; the optimum dose range for inactivation of WSSV and unaltered antigenicity was obtained 14–15 kGy. This preliminary information suggests that shrimp appear to benefit from treatment with gammairradiated WSSV especially at 14–15 KGy.  相似文献   

2.
This paper attempts to analyze in detail the remote influence of the Indian Ocean Basin warming on the Northwest Pacific (NWP) during the year of decaying E1 Nifio. Observation data and the Fast Ocean- Atmosphere coupled Model 1.5 were used to investigate the triggering conditions under which the remote influence is formed between the positive sea surface temperature (SST) anomaly in the North Indian Ocean and the Anomalous Northwest Pacific anticyclone (ANWPA). Our research show that it is only when there is a contributory background wind field over the Indian Ocean, i,e., when the Indian Summer Monsoon (ISM) reaches its peak, that the warmer SST anomaly in the North Indian Ocean incites significant easterly wind anomalies in the lower atmosphere of the Indo-West tropical Pacific. This then produces the remote influence on the ANWPA. Therefore, the SST anomaly in the North Indian Ocean might interfere with the prediction of the East Asia Summer Monsoon in the year of decaying E1 Nifio. Both the sustaining effect of local negative SST anomalies in the NWP, and the remote effect of positive SST anomalies in the North Indian Ocean on the ANWPA, should be considered in further research.  相似文献   

3.
Increasing evidence indicates that transforming growth factor β(TGF-P) signaling pathways play many important roles in the early development of mollusks.However,limited information is known concerning their detailed mechanisms.Here,we describe the identification,cloning and characterization of two Smad genes,the key components of TGF-P signaling pathways,from the Pacific oyster Crassostrea gigas.Sequence analysis of the two genes,designated as cgi-smad1/5/8 and cgi-smad4,revealed conserved functional characteristics.The two genes were widely expressed in embryos and larvae,suggesting multiple roles in the early development of C.gigas.The mRNA of the two genes aggregated in the D quadrant and cgi-smad4 was highly expressed on the dorsal side of the gastrula,indicating that TGF-P signaling pathways may be involved in dorsoventral patterning in C.gigas.Furthermore,high expression levels of the two genes in the shell fields of embryos at different stages suggested important roles for TGF-P signaling pathways in particular phases of shell development,including the formation of the initial shell field and the biomineralization of larval shells.The results of this study provide fundamental support for elucidating how TGF-P signaling pathways participate in the early development of bivalve mollusks,and suggest that further work is warranted to this end.  相似文献   

4.
Measuring Spatial Differences of Informatization in China   总被引:1,自引:1,他引:0  
As the wide application of new Information and Communication Technologies (ICTs) shows, the world is moving fast to- wards an information age. Since China was first connected to the Internet in 1994, the development of ICTs in China and around the world has been astonishingly fast, and yet there is a clear 'digital divide' among different regions in China. Although Chinese geogra- phers have paid attention to regional differences in informatization, they usually employ a limited number of indicators, mainly focusing on the Intemet. In fact, infi~rmatization is a much broader concept, covering not only the lntemet, but also mobile phones as well as user ability. In the light of these considerations, this study provides a comprehensive examination of the development of informatization and its spatial differences in China. First, based on a literature review, the paper identifies 29 preliminary indicators for measuring informa- tization, and employs prin,-ipal components analysis and the fuzzy analytic hierarchy process to streamline them into 12 indicators to form an ICTs Developmen: Index (ID1). Second, by using the data from provincial statistical yearbooks and the China Internet Network Information Center, the paper calculates the 1191 of each mega-region and each province in 2000-2010, and measures the changing spa- tial differences in the development of informatization in China. Lastly, the paper quantifies the relationship between informatization and economic growth. The empirical results show that the IDI of the western and central China has been increasing faster than that of the coastal region, indicating that the digital divide in China has been narrowing.  相似文献   

5.
This paper reports the concentrations of 137 Cs, hexachlorocyclohexane(HCH), dichlorodiphenyltrichloroethanes(DDT) and its main degradation products, δ13C, and organic carbon in pond sediments(0-210 cm, sectioned by 2-20 cm interval) and surface soils(the 0-3 cm horizon) collected in 2010 from Chenjia catchment, which is located in Yanting county in the hilly central Sichuan of China. α-, δ-, and γ-HCH, DDT, and DDD were not detected throughout the sediment profile. Trace concentrations of δ-HCH(0.89-29.31 ng g-1) and p,p′-DDE(1.85-6.02 ng g-1) were detected only in top 40 cm sediment. The 137 Cs fallout peak in 1963(corresponding to the 55-60 cm depth), the sedimentary signature left by the last year of HCH use in 1989(an additional indicator at 20-25 cm), and the obvious original channel bed prior to the construction of the pond in 1956 were used as temporal markers to estimate changes in average sedimentation rate between different periods due to changes in land use. Continuous, marked decrease in average sedimentation rate(i.e., 3.79, 1.35 and 1.07 cm year-1 in 1956-1963, 1963-1989, and 1989-2010, respectively)over time was observed, probably due to the reforestation, abandoning of steep sloping farmland for afforestation and natural re-vegetation(implementation of the Grain for Green Program), and the conversion of part of gently sloping farmland terraces to orchard land since the 1980 s, especially since the 1990 s. This was corroborated by the observed decrease(more negative) in δ13C of sediment towards the surface, which indicates increased relative contribution of eroded soil particles coming from slopes with increased tree cover in sediment source area. Combined use of 137 Cs, δ-HCH, and δ13C record in sediments has been demonstrated to be a powerful approach to reconstruction of response in sedimentation rate to historical land use changes.  相似文献   

6.
The velocity structure of the residual current across an entire section of the Qiongzhou Strait(QS) in summer is presented for the fi rst time. Shipboard Acoustic Doppler Current Profi le measurements, from the mid-region of the QS(110.18°E), were collected on 1–4 August 2010. The diurnal tidal currents had their maximum amplitudes between 4.24 and 20.24 m. Their amplitude along the major axis ranged from approximately 0.55 m/s in the middle part of the strait(20.15°N) to 0.84 m/s in the north part of the strait(20.20°N). Both anticlockwise and clockwise tidal current rotations exist in the QS. During the observation period(neap tide), a signifi cant westward residual current occupied almost the entire study section. Two velocity cores of westward current were observed at the northern part and near the deepest trough, although an eastward current appeared in the middle part of the transect. The deepest core was located near 62 m at 20.13°N, with a maximum velocity of-0.34 m/s. The shallower core was located at approximately 16 m at 20.20°N, with a maximum velocity of-0.33 m/s. The estimated total volume of water transported through the QS was-0.16 Sv. This value is an important boundary condition, applicable to numerical models studying coastal ocean circulation in the northwestern South China Sea.  相似文献   

7.
Traditional opinion considers that natural resources play an important positive role in economic development, while resource curse theory holds that natural resources usually obstruct economic increase. This debate needs further exploration. In most of empirical studies on resource curse theory, the economic development of an area is mainly evaluated by the Gross Domestic Product (GDP), however, the social and cultural contents of economic development are seldom considered. Thus, the Human Developing Index (HDI) was chosen to describe the comprehensive developing situation of an area in our study. Based on the panel data from the year of 2000 to 2011, the relationship between Human Developing Index and resource exploitation degree (RED) of 30 provinces in China (Tibet, Tai- wan, Hong Kong and Macao were not included because of the restriction of data acquisition) was investigated by correlation coefficient analysis and regression analysis. We found that resource curse did exist over the entire country and its effect on 30 provinces were not exactly the same. According to the effects of resource curse, these provinces could be classified into four types: no resource curse prov- inces, slight resource curse provinces, severe resource curse provinces, and extreme resource curse provinces. Testing from two short time periods 2000-2005, and 2006-2011, the resource curse effect was not prominent. However, testing from the entire period of 2000-2011, the effect was obvious among each province.  相似文献   

8.
A bstract In the present study,the effect of one-generation divergent selection on the growth and survival of the bay scallop(Argopecten irradians concentricus)was examined to evaluate the effi cacy of a selection program currently being carried out in Beibu Bay in the South China Sea.A total of 146 adult scallops were randomly selected from the same cultured population of A.i.concentricus,and divided into two groups in shell length(anterior-posterior measurement): large(4.91–6.02 cm,n=74)and small(3.31–4.18 cm,n=72).At the same time,a control group was also randomly sampled(4.21–4.88 cm,n=80).Mass-spawned F 1 progenies from the three size groups were obtained and reared under identical conditions at all growth phases.The effects of two-way(or upward-downward)selection on fertilization rate,hatching rate,survival rate,daily growth in shell length and body weight were assessed in the three size groups.Results show that signifi cant differences(P0.01)were found in hatching rate,survival rate and daily growth of F 1 progenies,but not in fertilization rate(P0.05),among the three groups.The hatching rate,survival rate and daily growth of the progeny of large-sized parents were greater than those of the control group(P0.05),which in turn were larger than those of small-sized group(P0.05).Responses to selection by shell length and body weight were 0.32±0.04 cm and 2.18±0.05 g,respectively,for the upward selection,and-0.14±0.03 cm and-2.77±0.06 g,respectively,for the downward selection.The realized heritability estimates of shell length and body weight were 0.38±0.06 cm and 0.22±0.07 g for the upward selection,and 0.24±0.06 cm and 0.37±0.09 g for the downward selection,respectively.The change in growth by bidirectional selection suggests that high genetic variation may be present in the cultured bay scallop population in China.  相似文献   

9.
In order to evaluate whether or not the county units' economy in the Changsha-Zhuzhou-Xiangtan (Chang-Zhu-Tan) Urban Agglomeration was growing as expected, this study analyzed the spatial economy pattern at county-level by using the Exploratory Spatial Data Analysis (ESDA) method. In this process, the global Moran's I and local Getis-Ord Gi* indexes were employed to analyze indicators including per capita GDP and three industrials (i.e. primary, secondary and tertiary industry) from 2000 to 2010. The results show that: 1) the county units' economy in the Chang-Zhu-Tan Urban Agglomeration has exhibited a strong spatial autocorrelation and an accelerated integration trend since 2008 (Moran' s I increased from 0.26 to 0.56); 2) there is a significant difference in economy development between the northern and southern county units in the Chang-Zhu-Tan Urban Agglomeration: the hotspot zone with high economic level was formed among the northern county units whereas the coldspot zone with low economic level was located in the southern areas. This difference was caused primarily by the increasingly prominent economic radiation effect of Changsha 'upheaval'; 3) town density, secondary industry, and the integration policy are the major contributors driving the evolution of the spatial economy pattern in the Chang-Zhu-Tan Urban Agglomeration.  相似文献   

10.
The toxic effects of benzo[α]pyrene (B[α]P) at different concentrations (0.1, 0.5, 1, 2.5 and 7.5 μgL^-1) on scallop (Chlamys farreri) immune system were studied. The results showed that B[α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial activity (P〈0.05), while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake, phagocytosis and bacteriolytic activity in all B[α]P treatment groups as well as antibacterial activity in groups of 0.5, 1, 2.5 and 7.5 μgL^-1·B[α]P decreased significantly (P〈0.05). Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear time- and concentration-response to B[α]E Bactenolytic activity in 0.1 μgL^-1 B[α]P treatment group and antibacterial activity in 0.1 ggLl and 0.5 μgL^-1 B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were significantly lower than those in control group (P〈0.05), except for antibacterial activity in 0.1 μgLl B[α]P treatment group (P〉0.05). Thus, B[α]P has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and lmmunomodulation in aquatic organisms.  相似文献   

11.
During the summer of 2011,a severe drought event occurred throughout the Zhujiang(Pearl)River Basin of South China.This decreased runoff into the river,resulting in increased salinity and reduced suspended substance.To examine the effects of this extreme drought on the distribution of nutrients and chlorophyll,we compared two surveys from 2006 and 2011.Results show that dissolved inorganic nitrogen concentration did not change from 2006 to 2011,whereas the proportions of NO 2 – and NH 4 + to DIN in 2011 increased.PO 4 3-concentration was lower in 2011 than in 2006,whereas there was no difference in SiO 3 2-concentration between these years.Correlation coeffi cients of salinity with levels of NO 3 –,NO 2 –,NH 4 +,PO 4 3-and SiO 3 2-in 2011 were all much higher than those in 2006,suggesting greater conservation of dissolved nutrients during the extreme drought event.Furthermore,calculated amounts of regenerated nitrate and phosphorus and their proportions to observed nutrients in 2011 were much lower than in 2006,indicating that nutrient regeneration decreased during the extreme drought period.Mean concentration of chlorophyll a(Chl-a)was considerably higher in 2011 than in 2006,and a harmful algal bloom of Cochlodinium geminatum was observed in the estuary,owing to water stagnancy and lower turbidity as a consequence of drought.Therefore,the extreme drought event in 2011 changed the composition ratio of nutrients,enhanced nutrient conservative behavior,and reduced nutrient regeneration.This affected some key ecological processes in the estuary.  相似文献   

12.
DAX 1,a member of nuclear receptor superfamily,has a function in the sex determination and gonadal differentiation of several vertebrate species.However,little information about DAX1 of invertebrates is available.Here we cloned a homolog of scallop (Chlamys farreri Jones and Preston 1904) dax1,Cf-dax1,and determined its expression characteristics at mRNA and protein levels.The cDNA sequence of Cf-dax1 was 2093 bp in length,including 1404 bp open reading frame (ORF) encoding 467 amino acids.Unlike those of vertebrates,no conserved LXXLL-related motif was found in the putative DNA binding region of Cf-DAX1.Fluorescence in situ hybridization showed that Cf-dax1 located on the short arm of a pair of subtelocentric chromosomes.Tissue distribution analysis using semi-quantitative RT-PCR revealed that Cf-dax1 expressed widely in adult scallop tissues,with the highest expression level found in adductor muscle,moderate level in mantle,gill and testis,and low level in kidney,ovary and hepatopancreas.The result of quantitative real-time PCR indicated that the expression of Cf-dax1 was significantly higher (P<0.05) in testis than in ovary at the same stage,showing a sex-dimorphic expression pattern.Furthermore,immunohistochemical detection found that Cf-DAX1 mainly located in spermatogonia and spermatocytes of testis and in oogonia and oocytes of ovary,implying that DAX1 may involve in gametogenesis of bivalves.  相似文献   

13.
Traditional eco-agriculture provides many ecosystem services which are important for the local environment, especially in mountain areas. In order to encourage the farmers to engage in eco-agriculture,it is necessary to establish an eco-compensation mechanism for them. As the compensation standard is one of key issues in establishing eco-compensation mechanism, this paper calculated the standard based on the farmers' willingness to accept(WTA) and the input-output analysis of eco-compensation. We took the traditional rice-fish eco-agriculture as the research object, conducting field surveys in Hani Terrace of Honghe County. Through the questionnaires in 2011, we obtained the farmers' willingness to accept government compensation and market compensation. Then, the research evaluated the output of eco-compensation, the economic value of rice-fish paddy ecosystem services. Finally, under different market compensation standards, we compared the input and output of government compensation. The results show that, in 2011 the government should to pay farmers 7462 yuan·ha–1·a-1to meet their willingness, but the output(ecological benefit) was only 7393 yuan·ha–1·a-1. However, when the rice price increases 1 yuan·kg-1 because of the limited use of chemicals in the next year, the government just has to pay farmers 4062 yuan·ha–1·a-1 and the surplus will be 3331 yuan·ha–1·a-1.  相似文献   

14.
Antarctic krill is a potentially nutritious food source for humans, but lfuorine (F) toxicity is a matter of concern. To evaluate the toxicity of F in Antarctic krill, 30 Wistar rats were divided into three groups with different dietary regimens:a control group, a krill treatment group (150 mg·kg-1 F), and a sodium lfuoride (NaF) treatment group (150 mg·kg-1 F). After three months, F concentrations in feces, plasma, and bone were determined, and the degree of dental and skeletal lfuorosis was assessed. The F concentrations in plasma and bone from the krill treatment group were 0.167 0±0.020 4 mg.L-1 and 2 709.8±301.9 mg·kg-1, respectively, compared with 0.043 8±0.005 5 mg·L-1 and 442.4±60.7 mg·kg-1, respectively, in samples from the control group. Concentrations of F in plasma and bone in the krill treatment group were higher than in the control group, but lower than in the NaF treatment group. The degree of dental lfuorosis in the krill treatment group was moderate, compared with severe in the NaF treatment group and normal in the control group. The degree of skeletal lfuorosis did not change signiifcantly in any group. These results showed that the toxicity of F in Antarctic krill was lower than for an equivalent concentration of F in NaF, but it was toxic for rats consuming krill in large quantities. To conclude, we discuss possible reasons for the reduced toxicity of F in Antarctic krill. The present study provides a direct toxicological reference for the consideration of Antarctic krill for human consumption.  相似文献   

15.
The generality to anisotropy of the earth media can be proved by a lot of research and observation, which is shown by the elastic parameters of the seismic wave changes with the direction of propagation. Actually, computational efficiency is very low, when simulating on the elastic anisotropie media, for the complicated and multi-parameters computation, which becomes a disadvantage to the succeeding migration and imaging pro-cedure. Using acoustic approximation in the VT[ media is an advisable simplification for the elastic wave simu-lation in that setting S-velocity into zero can greatly reduce the computational amount as well as get the same simulation effect. The authors get an acoustic approximation formula, then gain an anisotropic wave equation with 2-order in time and 4-order in space by inverse Fourier transformation, and dispel with the dispersion by adopting the high order finite difference operator in space and remove the edge reflection using the absorption attenuation boundary. Finally, the feasibility and effectiveness of this algorithm can be proved by the snaps and synthetic records.  相似文献   

16.
According to topography of Yemen, most areas and villages are located at obligated crest, toe of mountain and under cliffs. Therefore Al-Huwayshah consisting of Tawilah sandstone group is characterized by steep slope reach to 90℃ in some areas. This area is affected by strong tectonic movements and faults that occurred during the geological epochs. This effect enhances to find out fractures and joints as well as the rocks become brittle and ready to slide depending on the position of area. And there are some fractures and joints on the surface of the areas due to tectonic movement associated with opening the Red Sea and Gulf of Aden. The rainfalls, fractures, joints, earthquakes, gravity, vegetations, temperatures and human activities play a big role in the processes of rockfalls and landslides in this area. All those factors are considered as the basic causes and catalyzed factors for occurrence of rockfall in the studide area. In addition, the authors carried out laboratory test for many rock samples to get the physical properties of the rocks.  相似文献   

17.
The authors studied the geochemistry, zircon U-Pb ages, Hf isotope of the granite porphyry and its petrogenesis and tectonic setting in the studied area. The zircon U-Pb dating indicates the formation of the granite porphyry is in Early Cretaceous ( 125.1 ± 1.5 Ma). The granite porphyry has high-SiO2 and alkali-rich features, which belongs to high-K talc-alkaline series rocks ( A/CNK = 0. 95%-1. 25% ). The analyses of trace elements show the characteristics of a swallow-shaped REE distribution pattern with enrichment in LREEs and most of LILEs and HFSEs, depletion in Ba, Sr, Nb, P and Ti, and especially strong depletion in Eu, which indicates the granite porphyry belongs to the aluminous A-type granite. Their εHf(t) range from 5.94 to 8.80 with Hf two-stage model ages (TDM2 ) of 620 Ma to 803 Ma. Combining with the regional tectonic background, we conclude that the source of the rocks is the new crust materials accreted from depleted mantle in Neoproterozoic and is the product of partial melting of middle and lower crustal rocks, which may be suffered from the dual impact of the subduction of the Palaeo-Pacific Ocean and the closure of the Mongol-Okhotsk Ocean.  相似文献   

18.
Small granitic intrusions occur in the progressive metamorphic belts of the Chinese Ahai orogen. SHRIMP U-Pb analyses were performed on zircons separated from a tonalite intrusion and yielded a concordant age of 409.6±3.7 Ma ( MSWD = 0.93 ), restricting the emplacement and crystallization of the tonalite intru- sion to the Early Devonian. Combined with the existing geological data of the contemporary large granitic plutons in the Chinese Altai orogen, the tonalite is considered to be formed together with other granitic rocks in a continental arc setting. The tonalite intrusion has consistent foliation with its country rocks, indicating the strain resuiting in the regional deformation should be very strong during or after the early Devonian.  相似文献   

19.
The study of artificial slope stability has been a key item of geological engineering projects. Though more evaluation methods are available, result of stability evaluation simulation does not explain the actual problem owing to the diversified geological engineering factors and complexity. The author made a detailed study based on surveys of large amount of geological engineering research on Donggang Power Plant slope project, discussed the comprehensive factors influencing the project, and gave analytical calculation and evaluation to the improved response surface of the slope project. The study result shows that the slope is stable, which can provide scientific basis for designing the slope.  相似文献   

20.
The authors studied the potential field boundary identification of the new technology in order to find out the possible fractures or contact zones using the following methods such as tilt derivative, horizontal derivative of tilt derivative, normalized standard deviation and normalized differential method. Combined with Euler deconvolution and small subdomain filtering, the actual data processing results show that these methods are all a- ble to identify wider range extending fractures and obtain abundant geological information. The horizontal derivative of tilt derivative and normalized differential method have a better resolution for the small cutting fractures and lacunae in the studied area. They provide a reliable basis for study of the cutting relationship between fractures.  相似文献   

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