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种植盐地碱蓬修复滨海盐渍土效果的研究 总被引：25，自引：0，他引：25
利用盐生植物——盐地碱蓬进行天津河口滨海盐碱地的生物修复。通过比较种植盐地碱蓬土壤和对照土壤的电导率、有机质和微生物数量的差异等研究了其修复效果。结果表明，种植区碱蓬根际土壤的电导率与对照土壤相比下降了13％；有机质和总氮与对照土壤相比分别增加43％和18％；根际土壤的微生物数量明显增加，放线菌和真菌分别比对照增加了5倍和16倍。根际微生物优势种群的盐耐受性结果显示，部分根际土壤的优势种群的盐耐受性明显下降，耐盐性较低的微生物种群已成为优势种群。以上结果表明，种植盐地碱蓬对改善滨海盐碱地的生态有明显的效果。 相似文献
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南极放线菌NJ-F2萃取物的抗菌活性初步研究 总被引：2，自引：0，他引：2
从南极第24次科学考察采集的海泥样品中分离获得放线菌NJ-F2,对NJ-F2发酵液的乙酸乙酯萃取物进行体外抗菌实验,并采用16S rDNA方法对NJ-F2进行系统发育分析.结果表明萃取物对金黄色葡萄球菌和枯草芽孢杆菌的最低抑菌浓度(MIC)、最低杀菌浓度(MBC)分别为0.25 mg/mL,5.0 mg/mL;而对大肠杆菌和产气杆菌的MIC、MBC分别为0.5 mg/mL,5.0 mg/mL,但对真菌没有抗菌作用;16SrDNA分析结果表明NJ-F2为链霉菌属(Streptomyces sp.). 相似文献
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从南极第24次科学考察采集的样品中分离出产低温脂肪酶菌株Psychrobacter sp.G,通过PlackettBurman法及响应面法对其产脂肪酶条件进行优化,得出最适条件为:蛋白胨0.75％、酵母粉0.2％、NaCl 2％、MgSO40.1％、(NH4)2SO4 0.1％、K2HPO40.2％、Tween-80 1.5％、培养温度15℃、培养时间72 h、装液量150 mL/500 mL、振荡转速150 r/min.在此条件下发酵的脂肪酶活力为16.5 U/mL. 相似文献
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为构建南极冰藻Chlamydomonassp.ICE-L的cDNA文库,提取对数生长期南极冰藻ICE-L的总RNA,以此为模板,通过PowerScript逆转录酶逆转录合成第一链cDNA;再以第一链cDNA产物为模板,用LD-PCR合成第二链cDNA。该cDNA产物经分级分离转入大肠杆菌中,即获得南极冰藻ICE-L的cDNA原始文库,其滴度为1.6×106cfu/mL。扩增后的cDNA文库的滴度为1.0×1010cfu/mL。用PCR方法测得文库的重组率大于97%,插入cDNA的长度为0.5~1.8 kb,0.9 kb以上插入片段占50%以上。取适量扩增文库稀释并铺平板,挑取72个独立菌落,对其中22个独立菌落所插入的cDNA进行测序,克隆到了一个具有5′和3′非编码区的40S ribosomalprotein S5全长基因序列,GenBank收录号为AM167929。 相似文献
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Phylogenetic analysis based on 16S rDNA of 8 strains of cultivable bacteria isolated from Arctic sea-ice was studied.The results showed that strain BJ1 belonged to genus Planococcus,which was a genus of low mole percent G+C gram-positive bacteria;strain BJ6 belonged to genus Burkholderia of β-proteobacteria and the rest 6 strain all belonged to γ-proteobacteria,of which strain BJ8 was a species of Pseudoalteromonas,strain BJ2-BJ5 and BJ7 were members of genus Psychrobacter.Phylogenetic analysis also indicated that bacteria of genus Psychrobacter of the isolates formed a relatively independent phylogenetic cluster in comparison with other bacteria belonged to genus Psychrobacter. 相似文献
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从中国第二次北极科学考察采集的海冰样品中分离到8种细菌,研究了盐度对其生长的影响。结果表明,8株海冰细菌对盐度有着广泛的适应性,在盐度为30-90的范围内均可较快生长,其中菌株BJ2、BJ3和BJ7的盐耐受性较强。进一步的研究表明,BJ2、BJ3和BJ7在0-150 的盐度范围内均有不同程度的生长,盐度为0-30时,菌株的生长最快;盐度为180时,这三株菌株也均有短暂的生长。随着盐度的增加,三株菌株生长的延迟期增加,到达生长稳定期的时间也相应增加。温度对菌株BJ2、BJ3和BJ7的盐耐受性也有着一定的影响,随着温度的升高, 菌株的生长速度变慢,但盐耐受性有所增加。 相似文献
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Phylogenetic analysis based on 16S rDNA of 8 strains of cultivable bacteria isolated from Arctic sea-ice was studied.The results showed that strain BJ1 belonged to genus Planococcus,which was a genus of low mole percent G C gram-positive bacteria;strain BJ6 belonged to genus Burkholderia of β-proteobacteria and the rest 6 strain all belonged to γ-proteobacteria,of which strain BJ8 was a species of Pseudoalteromonas,strain BJ2-BJ5 and BJ7 were members of genus Psychrobacter.Phylogenetic analysis also indicated that bacteria of genus Psychrobacter of the isolates formed a relatively independent phylogenetic cluster in comparison with other bacteria belonged to genus Psychrobacter. 相似文献
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Thirty-six strains of marine actinomycetes were isolated from a sample of marine sediment collected from the Yellow Sea and evaluated in terms of their inhibitory activity on the growth of Aspergillus parasiticus and the production of norsolorinic acid using dual culture plate assay and agar diffusion methods. Among them, three strains showed strong antifungal activity and were subsequently identified as Streptomyces sp. by 16S rRNA gene sequencing analysis. The supernatant from the fermentation of the MA01 strain was extracted sequentially with chloroform and ethyl acetate, and the activities of the extracts were determined by tip culture assay. The assay results show that both extracts inhibited mycelium growth and toxin production, and the inhibitory activities of the extracts increased as their concentrations increased. The results of this study suggest that marine actinomycetes are biologically important for the control of mycotoxins, and that these bacteria could be used as novel biopesticides against mycotoxins. 相似文献