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Ressi is a small (2.4 ha) forested catchment located in the Italian pre-Alps. The site became an experimental catchment to investigate the water fluxes in the soil–plant–atmosphere continuum and the impact of vegetation on runoff generation in 2012. The elevation of the catchment ranges from 598 to 721 m a.s.l. and the climate is humid temperate. The bedrock consists of rhyolites and dacites; the soil is a Cambisol. The catchment is covered by a dense forest, dominated by beech, chestnut, maple, and hazel trees. The field set up includes measurements of the rainfall in an open area, streamflow at the outlet, soil moisture at various depths and locations, and depth to water table in six piezometers at a 5- or 10-min interval. Samples of precipitation, stream water, shallow groundwater and soil water are collected monthly for tracer analysis (stable isotopes (2H and 18O), electrical conductivity and major ions), and during selected rainfall–runoff events to determine the contribution of the various sources to runoff. Since 2017, soil and plant water samples have been collected to determine the sources of tree transpiration. Data collected in the period 2012–2016 are publicly available. Data collection is ongoing, and the data set is expected to be updated on an annual basis to include the most recent measurements.  相似文献   
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The role of endogenous redox mediators has considerable importance in electron shuttling reactions and associated performance of microbial fuel cell. Single-chamber microbial fuel cell-II with dual air-cathode assembly (area = 18.84 cm2) supported highest bacterial (Pseudomonas aeruginosa) density (6.7 × 109) and active biomass [4.4 ± 0.3 mg cm?2 (carbon content = 0.48 ± 0.1 mg cm?2)] on anode thereby resulting in maximum production of redox metabolite, 5-methyl 1, hydroxy phenazine (301 ppm) and voltage (595 ± 5 mV) than similar cells with relatively less surface area of cathode. It was further revealed that 5-methyl 1, hydroxy phenazine production was positively correlated with chemical oxygen demand removal rate (77 ± 2.5%) and power generation (66.6 ± 2.2 mW cm?2) efficiency of single-chamber microbial fuel cell-II. Maximum power density of 258 ± 4.5 mW cm?2 was generated when reactor was supplemented with 2 ml crude extract of 5-methyl 1, hydroxy phenazine metabolite, whereas power output was about 229 ± 2.5 mW cm?2 when reactor was bio-stimulated with 1 ml pure extract of 5-methyl 1, hydroxy phenazine. With this concentration, the electrochemical response of mixed culture biofilm (sediment) was enhanced by 99.3%. However, further increase in concentration of endogenous mediator proved to be limiting on reactor performance. Pyrosequencing and phylogenetic analysis on the basis of partial 16S rRNA sequences demonstrated both culturable and unculturable bacterial species in anodic biofilm and relative abundance of family Pseudomonadaceae was found to be maximum, i.e., 61.7% followed by Rhodocyclaceae 19.2%, Xanthomonadaceae 6.3% and Opitutaceae 3.18%.  相似文献   
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链状亚历山大藻(Alexandrium catenella)是一种典型的产毒赤潮甲藻。甲藻曾被认为没有组蛋白,但近年来在多种甲藻中检测到全部四个核心组蛋白的活跃转录,而目前对甲藻中组蛋白表达模式与具体功能还缺乏深入的研究。本文报道了链状亚历山大藻组蛋白H3变体之一H3.c的全长ORF序列的克隆和分析;分析了链状亚历山大藻生长过程中组蛋白H3在基因和蛋白水平的表达。目前研究发现的链状亚历山大藻H3变体共三个,其中H3.b在N末端序列与其他物种差异最大,为链状亚历山大藻特有的H3变体。对数生长期的组蛋白H3在基因与蛋白水平上均活跃表达,但表达量并不会随着爆发性增长而显著变化,其中H3.b的基因表达在对数末期呈现上调;培养至衰亡期,各H3变体表达均下调,尤其在蛋白水平上呈现明显衰减。结合前期研究,本文认为链状亚历山大藻三个H3均为复制非依赖型(RI)变体,一些变体可响应伴随藻不断生长而逐渐增强的细胞密度等复杂胁迫,推测其参与某些表观遗传修饰,调控生长进程;而衰亡期基于H3的表观遗传调控受到抑制。  相似文献   
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