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顶空气相色谱法测定海水二甲基硫和浮游植物细胞二甲基硫丙酸的研究 总被引:12,自引:2,他引:12
建立了顶空GC/FPD测定海水中二甲基硫(DMS)和浮游植物细胞中二甲基硫丙酸(DMSP)的方法,并研究盐度、温度、气液相比DMS诸因素对DMS顶空灵敏度的影响。该法对DMS测定的相对标准偏差均小于6%,平均回收率为106%,最低检出限为20ng/L。细胞DMSP先经碱作用转化为DMS,在50℃下作用时间不少于6h,峰高与浓度的双对数线性相关系数大于0.99。对1994年冬、1995年夏采自胶州湾 相似文献
3.
厦门西海域浮游植物的生态 总被引:7,自引:5,他引:7
本文分析、鉴定了于1989年4月至1990年3月,逐月采自厦门西海域的7个站位的112份样品。结果表明,本海区浮游植物共129种,其中蓝藻1种,硅藻105种,金藻1种,甲藻22种。按其分布特点,可分为两大生态类群:广布种占57%,暖水种占43%。浮游植物细胞总量的季节分布是8月出现主高峰,5月为次高峰,3月是最低谷期。此外,对其数量分布和季节变化与海洋环境因素的关系,进行了较详细的讨论。 相似文献
4.
5.
Preservation conditions and the use of sediment pigments as a tool for recent ecological reconstruction in four Northern European estuaries 总被引:4,自引:0,他引:4
Down-core sediment pigment concentrations from four Northern European estuaries were measured using high-performance liquid chromatography (HPLC) to investigate phytoplankton community structure and preservation conditions over the last ca. 100 years where all sites have experienced different levels of eutrophication. Phytoplankton pigments have been shown to be useful biomarkers for phytoplankton community structure and abundance due to their taxonomic specificity. The pigment concentrations and sediment pigment inventory showed large variation between the four sites. Concentrations ranged from more than 6000 nmol/g OC to less than 100 nmol/g OC and the inventory integrated over the top 10 cm from more than 300 nmol/cm2 to less than 30 nmol/cm2 for total identified pigments. Good pigment preservation in Mariager Fjord (Denmark) reflected the almost permanently anoxic conditions. Pigments in Laajalahti (Finland) showed peak concentrations around the time of highest nitrogen loading events known from historical and modelled records over the past 100 years. In contrast, poor down-core preservation of pigments (especially carotenoids) was observed in the Ems-Dollard (The Netherlands) and Himmerfjärden (Sweden) estuaries. The Ems-Dollard site is an intertidal mudflat that experiences daily exposure to light and air, which enhances pigment degradation. In Himmerfjärden, resuspension is an important process affecting both the sedimentation rate and degradation properties. The different preservation conditions at the four sites were supported by the differences in two degradation indicators; the ratio of pheopigment-a to chlorophyll-a and total carotenoids to total pigments. Class-specific carotenoid pigments represented the dominant algal groups reported from each site, however, no distinct down-core changes in the pigment composition were observed at any of the four sites. This indicated that changes in plankton community structure on the group level have been limited over this time period or masked by low preservation of pigments. 相似文献
6.
Effects of cold eddy on phytoplankton production and assemblages in Luzon strait bordering the South China Sea 总被引:6,自引:0,他引:6
Yuh-Ling Lee Chen Houng-Yung Chen I. -I. Lin Ming-An Lee Jeng Chang 《Journal of Oceanography》2007,63(4):671-683
The biochemical effects of a cold-core eddy that was shed from the Kuroshio Current at the Luzon Strait bordering the South
China Sea (SCS) were studied in late spring, a relatively unproductive season in the SCS. The extent of the eddy was determined
by time-series images of SeaWiFS ocean color, AVHRR sea surface temperature, and TOPEX/Jason-1 sea surface height anomaly.
Nutrient budgets, nitrate-based new production, primary production, and phytoplankton assemblages were compared between the
eddy and its surrounding Kuroshio and SCS waters. The enhanced productivity in the eddy was comparable to wintertime productivity
in the SCS basin, which is supported by upwelled subsurface nitrate under the prevailing Northeastern Monsoon. There were
more Synechococcus, pico-eucaryotes, and diatoms, but less Trichodesmium in the surface water inside the eddy than outside. Prochlorococcus and Richelia intracellularis showed no spatial differences. Water column-integrated primary production (IPP) inside the eddy was 2–3 times that outside
the eddy in the SCS (1.09 vs. 0.59 g C m−2d−1), as was nitrate-based new production (INP) (0.67 vs. 0.25 g C m−2d−1). INP in the eddy was 6 times that in the Kuroshio (0.12 g C m−2d−1). IPP and INP in the eddy were higher than the maximum production values ever measured in the SCS basin. Surface chlorophyll
a concentration (0.40 mg m−3) in the eddy equaled the maximum concentration registered for the SCS basin and was higher than the wintertime average (0.29
± 0.04 mg m−3). INP was 3.5 times as great and IPP was doubled in the eddy compared to the wintertime SCS basin. As cold core eddies form
intermittently all year round as the Kuroshio invades the SCS, their effects on phytoplankton productivity and assemblages
are likely to have important influences on the biogeochemical cycle of the region. 相似文献
7.
This paper brings together unpublished historical data sets and published literature to review the role of climatic, oceanographic and ecological processes in the marine ecosystem of the eastern Canadian Archipelago. Physical data include characteristics of the water masses, circulation patterns, sea ice conditions, and climatic records from 1950s onward. Biological data include unpublished data sets on nutrients, primary and secondary production, and sedimentation, which were collected during the 1980–1990s in the eastern Canadian Archipelago. These results show high year-to-year variability in nutrient inventories and ratios, the magnitude of the ice algae and phytoplankton bloom, the timing of ice algae sedimentation in the spring, and the composition of the zooplankton community. The significance of this high interannual variability and its effect on pelagic–benthic coupling processes is discussed in the context of climatic and oceanographic forcing, with emphasis on recent (past decade) Arctic changes. An estimate of total primary production in the Archipelago is also presented, along with published production estimates for other Arctic shelves, showing that the Archipelago may support up to 32% of the total primary production of Arctic shelves. The high year-to-year variability in production and carbon transfer pathways (e.g. pelagic versus benthic) in the Archipelago suggest that the system might be resilient to the increased variability in climatic conditions occurring in the past decade. However, this increased variability combined with directional change in climatic and oceanographic conditions might also modify the existing balance of ecological processes. For example, shifts in the timing of events appear to have already occurred in the past decade, with potential cascading effects throughout the ecosystem. 相似文献
8.
A new class of phytoplankton models with a mechanistic basis has been presented in a companion paper (Baklouti, M., Diaz, F., Pinazo, C., Faure, V., Queguiner, B., 2006. Investigation of mechanistic formulations depicting phytoplankton dynamics for models of marine pelagic ecosystems. Progress in Oceanography). It is the default class of models implemented in our new numerical tool Eco3M, which is dedicated to Ecological, Mechanistic and Modular Modelling. A brief overview of its main features is given in Section 2 of the present paper. In the next sections, a particular phytoplankton model among the aforementioned class has been tested with special emphasis on the mechanistic photosynthesis component relating the photosynthetic rate to the proportion of open photosystems II. The present study encompasses several essential steps that are inherent to any modelling, including model reduction, model sensitivity analysis and comparison of model outputs with experiments. The global sensitivity analysis of the plankton model for one-at-a-time parameter perturbations revealed a restricted set of parameters having major influence on the model outputs. Sensitivity tests involving simultaneous parameter perturbations within the range actually encountered in the literature provided a confidence interval for the outputs. Chemostat experiments performed on nitrate-limited diatoms grown under low (LL) and high-light (HL) conditions have been used for comparison with model outputs. The good fit between measured data and model outputs using the same parameter values in both the LL and HL cases demonstrates the ability of our model to represent the main features of phytoplankton dynamics including photoacclimation. Finally, Eco3M is ultimately intended to include explicit bacterial and zooplankton compartments, as well as to be coupled with ocean circulation models, but the intrinsic behavior of the phytoplankton model has been investigated first, independently of physical forcing. 相似文献
9.
2001-2002年天目湖(沙河水库)浮游植物的生态学研究 总被引:7,自引:0,他引:7
2001年6月~2002年5月,对天目湖进行的浮游植物周年调查表明,天目湖共有浮游植物7门75属,其中年平均丰度为5026.20×104个/L,以蓝藻为主,占总丰度的54.45%;而年平均生物量为(15.364±9.103)mg/L,其中硅藻居首位,为6.634 mg/L,占浮游植物年平均生物量的43.18%;其周年变化是丰度和生物量最高值均出现在5月,丰度在5月和9月出现2个峰值,而生物量则在5、7、10月出现3个峰值。浮游植物丰度从大坝处1#点到河流入湖口的9#、10#点变化不明显但略有增加,而生物量则呈明显增加。通过对水温、透明度、营养盐与浮游植物丰度和生物量的线性回归分析发现,浮游植物丰度、生物量与水温、TP存在显著的正相关,而与N/P比、透明度存在显著性负相关,与TN相关性不是很明显。 相似文献
10.
Christopher L. Dupont James. W. Moffett Robert R. Bidigare Beth A. Ahner 《Deep Sea Research Part I: Oceanographic Research Papers》2006,53(12):1961-1974
Dissolved and particulate concentrations of the biogenic thiols cysteine (Cys), arginine–cysteine (Arg–Cys), glutamine–cysteine (Gln–Cys), γ-glutamate–cysteine (γ-Glu–Cys) and glutathione (GSH) were measured in the subartic Pacific Ocean in the summer of 2003 using high performance liquid chromatography (HPLC) with precolumn derivatization as reported in previous work. In this study, a preconcentration protocol for the derivatized thiols was utilized to extend detection limits of dissolved thiols to picomolar levels. The measured concentrations of particulate and dissolved thiols were uncoupled, with distinctive depth profiles and large differences in the particulate to dissolved ratios between individual compounds. Glutathione was the most abundant particulate thiol whereas the most abundant dissolved thiol was γ-Glu–Cys, with concentrations as high as 15 nM. Given the relatively small pool of intracellular γ-Glu–Cys and the very low dissolved concentrations of GSH, we hypothesize that glutathione released from cells is rapidly converted to the potentially degradation resistant γ-Glu–Cys outside the cell. The relatively high concentrations of other dissolved thiols compared to particulate concentrations implies both biological exudation and slow degradation rates. Some thiols appear to vary with changes in nutrient availability but this effect is difficult to decouple from changes in community structure inferred from pigment analyses. Dissolved thiol concentrations also exceed typical metal concentrations in the subartic Pacific, supporting previous arguments that they may be important in metal speciation. 相似文献