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采用基因克隆技术构建双元载体pCAMBIA2301-idi,通过电转转入农杆菌LBA4404中.利用根癌农杆菌介导的转化方法,将pCAMBIA2301-idi质粒的T-DNA区转入小球藻,以G418抗性基因(NPTⅡ)作为筛选标记,筛选出阳性转化子.通过PCR扩增表明idi基因和NPTⅡ基因已经整合到小球藻基因组中.测定转化子的生物量,结果表明大部分转化子的生物量与野生型相似.测定转化子小球藻干粉的叶黄素含量,发现转化子叶黄素含量最高达到0.84mg/g,与野生型相比提高了30.95％.进一步分析藻液中叶黄素的产量,发现转化子的叶黄素产量最高达到1.98mg/L,比野生型提高了36.77％. 相似文献
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利用闽北某区（带）地球化学普查中２幅１：５万水系沉积物测量数据,分别进行单变量（元素）数据处理和多变量（元素）广义幂转换－因子分析数据处理,取得较好的地质成果,清晰,简明地反映区内各元素的分布规律和异常集中地段,将成晕元素进行有机组合,以单变量形式表达出来,使某些有价值的弱信息强化,更客观“真实”地展现区域成矿潜力。 相似文献
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The metabolic pathway of phenanthrene-degrading strain Agrobacterium sp. Phx1 was investigated. Phx1 almost was able to transform 100 υg/mL of phenanthrene completely in 1 day in broth media of beef extract-peptone (BP), Luria-Bertani (LB) and mineral salts media (MS), and LB and BP could promote the growth and degradation efficiency of Phx1. The GC-MS was employed to analyze the metabolites of the 1st, 3rd, 7th days of phenanthrene degradation in MS. As a result, the 1-Hydroxy-2-naphthoic acid (1H2N) and 1-naphthol (NOL) were detected in the metabolites of the 1st day. Only NOL was observed on the 3rd day and it disappeared on the 7th day. The accumulated NOL did not pertain to the defined pathway of phenanthrene degradation by bacteria. The further HPLC study confirmed the finding in GC-MS analysis and found the production of catechol (CAT) from o-phthalic acid (OPA) in the phenanthrene metabolizing, which has never been reported in the defined degrading pathways. This production was also evidenced by the production of CAT using OPA as substrate. All of our results showed that the Agrobacterium sp. Phx1 had a novel phenanthrene-degrading pathway. 相似文献
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