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1.
The toxic effects of benzo[α]pyrene (B[α]P) at different concentrations (0.1, 0.5, 1, 2.5 and 7.5 μgL−1) on scallop (Chlamy farreri) immune system were studied. The results showed that B[α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial
activity (P<0.05), while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake,
phagocytosis and bacteriolytic activity in all B[α]P treatment groups as well as antibacterial activity in groups of 0.5, 1, 2.5 and 7.5 μgL−1 B[α]P decreased significantly (P<0.05). Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear
time- and concentration-response to B[α]P. Bacteriolytic activity in 0.1μgL−1 B[α]P treatment group and antibacterial activity in 0.1 μgL−1 and 0.5 μgL- B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following
that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were
significantly lower than those in control group (P<0.05), except for antibacterial activity in 0.1 μgL−1 B[α]P treatment group (P>0.05). Thus, B[α] has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution
and immunomodulation in aquatic organisms. 相似文献
2.
光镜观察湛江南三地区文蛤血细胞的形态与结构特征,并进行分类。按细胞大小、形态和结构特征可将文蛤血细胞分为4类:透明细胞、小颗粒细胞、大颗粒细胞和淋巴细胞。细胞直径有统计学上显著性差异(P<0.05),分别为10.11±0.44μm、10.99±0.32μm、9.63±0.41μm和9.22±0.25μm。透明细胞是血细胞中的主要成分,细胞质中无颗粒;小颗粒细胞的细胞质中存在大量细小颗粒;大颗粒细胞细胞质中的颗粒直径有1.0～2.5μm;淋巴细胞是血细胞中含量最少的,有很高的核质比,大约为0.5。 相似文献
3.
日本七鳃鳗外周血细胞显微结构及类淋巴细胞体外培养 总被引：1，自引：0，他引：1
以日本七鳃鳗(Lampetra japonica)为研究对象,观察其外周血细胞显微结构和探讨类淋巴细胞原代培养条件。采用Wright氏和Giemsa氏染色法对日本七鳃鳗外周血液有形成分进行显微观察,可鉴别出红细胞、淋巴细胞、中性粒细胞、单核细胞、嗜酸性粒细胞;未发现嗜碱性粒细胞。通过Ficoll密度梯度离心法和流式细胞仪分选获得高纯度的七鳃鳗类淋巴细胞。经条件优化后确定最适培养液L15+20%FBS(5%七鳃鳗血清)+0.46%NaCl,在18℃,pH 6.8-7.0的条件下对日本七鳃鳗类淋巴细胞进行体外培养,大多数类淋巴细胞半贴壁生长,细胞状态较好,最长可以存活近一个月。类淋巴细胞的原代培养为日本七鳃鳗细胞体外培养的深入研究提供了实验依据。 相似文献
4.
于2009年3月至2010年1月期间,每月中旬分别从青岛和威海两地区采集人工养殖和野生的栉孔扇贝(Chla-mys farreri)。采集的扇贝经壳长测量后,于闭壳肌血窦中取血,离心,重悬,超声波破碎后制得血细胞破碎液。从抗栉孔扇贝血细胞的单克隆抗体库中筛选出1株与颗粒血细胞和透明血细胞均能结合,并能与血细胞多个蛋白结合的单克隆抗体作为酶联免疫吸附法(ELISA)中的第一抗体;第二抗体为碱性磷酸酶标记的羊抗鼠抗体。将血细胞破碎液包被于酶标板孔中,经一抗、二抗孵育后,显色读数,分析血细胞数量与生长的关系。结果表明:两地区人工养殖和野生扇贝的壳长均由3月的2 cm左右增长到了次年1月的7 cm以上,其中4～7月增长较快,而8～1月相对缓慢。两地区人工养殖和野生扇贝的血细胞数量于3～6月间均维持在较高的水平,6月达到最高峰,随后急剧下降,并于8或9月达到最低值,此后10～1月有所回升,但仍显著低于3～6月的水平。结论认为:栉孔扇贝血细胞数量与生长的关系在两地区、两扇贝品种间差别不大;但3～6月栉孔扇贝生长较快,血细胞数量相对高;而8～10月栉孔扇贝生长缓慢,其血细胞数量较低。 相似文献
5.
The toxic effects of benzo[α]pyrene (B[α]P) at different concentrations (0.1, 0.5, 1, 2.5 and 7.5 ugL-1) on scallop (Chla-mys farreri) immune system were studied. The results showed that B[a]P had significant toxic effects on the haemocyte counts, neu-tral red uptake, phagocytosis, bacteriolytic and antibacterial activity (P<0.05), while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake, phagocytosis and bacteriolvtic activity in all B[α]P treatment groups as well as antibacterial activity, in groups of 0.5, 1, 2.5 and 7.5 ugL-1 B[α]P decreased significantly (P<0.05). Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear time- and concentra-tion-response to B[α]P. Bacteriolytic activity in 0.1ugL-1 B[α]P treatment group and antibacterial activity in 0.1 ugL-1 and 0.5 ugL-1 B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were significantly lower than those in control group (P<0.05), except for antibacterial activity in 0.1 ugL-1 B[α]P treatment group (P<0.05). Thus, B[α]P has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and immu-nomodulation in aquatic organisms. 相似文献
6.
《海洋科学》2012,36(1)
以日本七鳃鳗（Lampetrajaponica）为研究对象,观察其外周血细胞显微结构和探讨类淋巴细胞原代培养条件。采用Wright氏和Giemsa氏染色法对日本七鳃鳗外周血液有形成分进行显微观察,可鉴别出红细胞、淋巴细胞、中性粒细胞、单核细胞、嗜酸性粒细胞：未发现嗜碱性粒细胞。通过Ficoll密度梯度离心法和流式细胞仪分选获得高纯度的七鳃鳗类淋巴细胞。经条件优化后确定最适培养液L15＋20％FBS（5％七鳃鳗血清）＋0．46％NaCl,在18℃,pH6．8～7．0的条件下对日本七鳃鳗类淋巴细胞进行体外培养,大多数类淋巴细胞半贴壁生长,细胞状态较好,最长可以存活近一个月。类淋巴细胞的原代培养为日本七鳃鳗细胞体外培养的深入研究提供了实验依据。 相似文献
7.
The toxic effects of benzo[α]pyrene （B[α]P） at different concentrations （0.1, 0.5, 1, 2.5 and 7.5 μgL^-1） on scallop （Chlamys farreri） immune system were studied. The results showed that B[α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial activity （P〈0.05）, while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake, phagocytosis and bacteriolytic activity in all B[α]P treatment groups as well as antibacterial activity in groups of 0.5, 1, 2.5 and 7.5 μgL^-1·B[α]P decreased significantly （P〈0.05）. Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear time- and concentration-response to B[α]E Bactenolytic activity in 0.1 μgL^-1 B[α]P treatment group and antibacterial activity in 0.1 ggLl and 0.5 μgL^-1 B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were significantly lower than those in control group （P〈0.05）, except for antibacterial activity in 0.1 μgLl B[α]P treatment group （P〉0.05）. Thus, B[α]P has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and lmmunomodulation in aquatic organisms. 相似文献
8.
9.
In this study, we investigated the effects of low salinity(26 and 21)on the prophenoloxidase(proPO)system and related defence parameters in the shrimp Litopenaeus vannamei. The results showed that low salinity induced a significant increase of dopamine(DA)concentration in haemolymph at 6h of the experiment; on the other hand, total haemocyte count(THC), differential haemocyte count(DHC)and PO activity decreased over time to the lowest level at 24 h and remained low thereafter. Serine Protease(SP)and Proteinase Inhibitor(PI)activity in the two lower salinity treatments decreased to the lowest level at 12 and 24h, respectively, and both recovered to the control level at 72 h. In contrast, α2-macroglobulin(α2M)activity in the two lower salinity treatments peaked at 24h and then decreased to the control level at 72h. Therefore, it may be concluded that stress-induced DA plays an important temporary role in neurotransmission and causes immune response in L. Vannamei in adapting to salinity changes. 相似文献
10.
研究组前期实验发现,栉孔扇贝血细胞在D形幼虫时期出现.本文应用单克隆抗体的免疫组化、免疫电镜方法进一步定位栉孔扇贝壳顶幼虫的血细胞并观察其分布.结果表明,栉孔扇贝壳顶幼虫已分化出界限明显的组织器官,包括外套膜、面盘、口、食道、胃、消化腺、足等;血细胞主要分布于壳顶幼虫的外套膜、面盘、食道、消化腺、胃等组织器官内及周围,其中面盘、消化腺、胃等处有大量血细胞成簇分布.血细胞形状不规则,直径为3～5μm;细胞核多为圆形、椭圆形,位于细胞的一侧;细胞质内有线粒体、内质网等细胞器和空泡.结果证实,栉孔扇贝在壳顶幼虫时期已出现了大量清晰可辨的血细胞,其分布特点与成贝血细胞相似. 相似文献