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Phylogenomic analysis of transcriptomic sequences of mitochondria and chloroplasts for marine red algae (Rhodophyta) in China 总被引：1，自引：1，他引：0
JIA Shangang WANG Xumin QIAN Hao LI Tianyong SUN Jing WANG Liang YU Jun LI Xingang YIN Jinlong LIU Tao WU Shuangxiu 《海洋学报(英文版)》2014,33(2):86-93
The chloroplast and mitochondrion of red algae （Phylum Rhodophyta） may have originated from different endosymbiosis. In this study, we carried out phylogenomic analysis to distinguish their evolutionary lin-eages by using red algal RNA-seq datasets of the 1 000 Plants （1KP） Project and publicly available complete genomes of mitochondria and chloroplasts of Rhodophyta. We have found that red algae were divided into three clades of orders, Florideophyceae, Bangiophyceae and Cyanidiophyceae. Taxonomy resolution for Class Florideophyceae showed that Order Gigartinales was close to Order Halymeniales, while Order Graci-lariales was in a clade of Order Ceramials. We confirmed Prionitis divaricata （Family Halymeniaceae） was closely related to the clade of Order Gracilariales, rather than to genus Grateloupia of Order Halymeniales as reported before. Furthermore, we found both mitochondrial and chloroplastic genes in Rhodophyta under negative selection （Ka/Ks〈1）, suggesting that red algae, as one primitive group of eukaryotic algae, might share joint evolutionary history with these two organelles for a long time, although we identified some dif-ferences in their phylogenetic trees. Our analysis provided the basic phylogenetic relationships of red algae, and demonstrated their potential ability to study endosymbiotic events. 相似文献
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ImODUcrlONTheultmetruCtureofthespermatosoainteeostfisheshaddridesedtalandcomParedpelllard,l97o,Mattei,l97O).ComPatalwithotherVertebrausryisheIson,l995),FisheShavegreaterdriversityinspermatosoaultfastruCture,indudingthemorphologicalcharaCteristicaofthesperm-headsophnsteinerandPatZner,l99O),thefonnswithorwithoutacrosorapeisheIson,l995),thenumberoftheflagellum,andthedistinguishingfeaturesofAntochondrialderivatives(JawhesonandGrier,l993).AllthesearereataltotheFishposi-tioninevolutionandt… 相似文献
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由于近年来过度捕捞及滩涂开发,野生矛尾复虾虎鱼资源量急剧下降.因此,对我国矛尾复虾虎鱼开展遗传多样性研究,有利于掌握矛尾复虾虎鱼种质资源现状.本文基于线粒体COI序列对大连、如东、连云港、长江口和宁波的5个矛尾复虾虎鱼群体开展了遗传多样性研究.结果 表明,5个群体中共有17个单倍型,在427个位点中,共检测到15个变异位点,其中简约信息位点11个,单独位点4个.碱基A、T、G、C的平均含量分别为24.6％、29.6％、20.1％、25.7％.5个群体的单倍型多样性和核苷酸多样性分别为0652和000255.野生矛尾复虾虎鱼整体的遗传多样性水平为中等偏下,宁波群体的遗传多样性最高,如东群体最低.群体间遗传分化系数FST及遗传距离分析表明,宁波群体与其他群体遗传距离相对较远,并存在显著的遗传分化;AMOVA分析表明,群体内的遗传变异高于群体间变异(81.45％＞ 18.55％);中性检验结果显示,大连、连云港、长江群体在历史上可能经历过扩张.本研究为野生矛尾复虾虎鱼种质资源保护和合理开发利用提供了理论依据. 相似文献
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Phylogenomic analysis of transcriptomic sequences of mitochondria and chloroplasts of essential brown algae (Phaeophyceae) in China 总被引：1，自引：1，他引：0
JIA Shangang WANG Xumin LI Tianyong QIAN Hao SUN Jing WANG Liang YU Jun REN Lufeng YIN Jinlong LIU Tao WU Shuangxiu 《海洋学报(英文版)》2014,33(2):94-101
The chloroplast and mitochondrion of brown algae （Class Phaeophyceae of Phylum Ochrophyta） may have originated from different endosymbiosis. In this study, we carried out phylogenomic analysis to distinguish their evolutionary lineages by using algal RNA-seq datasets of the 1 000 Plants （1KP） Project and publicly available complete genomes of mitochondria and chloroplasts of Kingdom Chromista. We have found that there is a split between Class Phaeophyceae of Phylum Ochrophyta and the others （Phylum Cryptophyta and Haptophyta） in Kingdom Chromista, and identified more diversity in chloroplast genes than mitochondrial ones in their phylogenetic trees. Taxonomy resolution for Class Phaeophyceae showed that it was divided into Laminariales-Ectocarpales clade and Fucales clade, and phylogenetic positions of Kjellmaniella crassi-folia, Hizikia fusifrome and Ishige okamurai were confirmed. Our analysis provided the basic phylogenetic relationships of Chromista algae, and demonstrated their potential ability to study endosymbiotic events. 相似文献
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为探讨淡水(盐度为0)对斜带石斑鱼(Epinephelus coioides)幼鱼鳃线粒体丰富细胞(MRCs)形态结构的影响,进一步探究其对鳃MRCs渗透调节机制的影响,以盐度30为对照,在斜带石斑鱼幼鱼急性淡水胁迫过程中,对鳃MRCs的结构变化进行光学显微镜(2、6、12、48 h)和扫描电镜观察(48 h).结果显示:淡水组鳃MRCs长径和短径均显著小于对照组(p0.05),H.E染色较浅;鳃小片直径显著变宽(p0.05),且鳃小片上增生出鳃MRCs;鳃MRCs表面存在一个特殊的顶膜结构,淡水组顶膜较为平坦,开口较大,且其表面存在大量微绒毛,对照组顶膜向内凹陷成一个开口较小的顶隐窝.推测在急性淡水胁迫下,斜带石斑鱼鳃MRCs的结构发生变化,导致其渗透调节功能部分丧失,幼鱼死亡,所以斜带石斑鱼幼鱼直接转入淡水时,不能长期存活. 相似文献
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Transmission electron microscopy of the yellowfin porgy (Sparus latus Houttuyn) spermatozoa ultrastructure showed the spermatozoon as a primitive type made up of the acrosomeless head, the flagellum,
and the midpiece, at the periphery of which was a relatively big mitochondrion with more complex structure. It was found that
during spermiogenesis, only one relatively big mitochondrion occurred in both the spermatid and the spermatozoon. This is
different from other teleost fishes. During spemiogenesis, the mitochondria number is one, and morphology did not change.
All these are different from those of other fishes. 相似文献
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紫红笛鲷线粒体全序列与笛鲷属鱼类分化年代的贝叶斯分析 总被引：1，自引：0，他引：1
利用常规 PCR 未纯化产物直接测序的方法,获得紫红笛鲷(Lutjanus argentimaculatus)全长为16543 bp 线粒体 DNA 全序列, GenBank 登录号为 JN182927.结合 GenBank 中的已有数据比较分析表明:紫红笛鲷与笛鲷属(Lutjanus)线粒体基因组成基本一致,符合脊椎动物的特征,各基因间进化速率差异显著;13个蛋白编码基因拼接序列贝叶斯建树的后验率显著高于单基因或全序列,更适用于笛鲷属的贝叶斯法系统分化分析和年代推断;13个蛋白编码基因及其拼接序列进行的系统进化分析均支持紫红笛鲷与勒氏笛鲷(Lutjanus russellii)及海鸡母笛鲷(Lutjanus rivulatus)亲缘关系较近;基于13个蛋白编码基因拼接序列的贝叶斯松散分子钟推算出笛鲷属鱼类两个主要支系大约在61.5Ma 前古新世的达宁阶与蒙蒂阶交界时期发生分化. 相似文献
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多棘海盘车(Asterias amurensis)是海星纲的一个致灾物种,广泛分布于包括我国黄海、渤海海域、韩国、日本和俄罗斯海域在内的北太平洋海域,并作为入侵种分布于澳大利亚的塔斯马尼亚海域。多棘海盘车在多个海域发生过暴发性增殖导致的高密度、大规模聚集,对海洋生态系统和水产养殖造成严重的负面影响。自2006年以来在我国山东青岛海域多次发生大规模多棘海盘车聚集,对贝类养殖造成重大经济损失。日本、俄罗斯、澳大利亚等海域的多棘海盘车遗传多样性研究取得了显著进展,然而,迄今为止鲜有针对我国海域致灾物种多棘海盘车的分子生物学分析,阻碍了我国海域种群与全球种群间遗传进化关系的比较分析。研究重点分析了于2022年7月在胶州湾发生多棘海盘车暴发时采集的12个样本,系统组装了其线粒体基因组、核糖体基因簇以及多种通用分子标记。基于线粒体基因组和核糖体基因簇的系统发育分析表明多棘海盘车具有较高的种内遗传多样性。基于线粒体基因组的蛋白编码基因进行分化时间估算,多棘海盘车的种内遗传分化可能发生在1.7～4.2 Ma,表明多棘海盘车的扩布可能较早发生。与线粒体基因组相比,多棘海盘车核糖体基因簇序列在种内极为保守... 相似文献
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