基于电子鼻的青蛤(Cyclina sinensis)软组织多氯联苯的快速检测方法研究     

吕燕  王祖忠  胡玲萍  黄健  周君  张春丹  李晔  苏秀榕 《海洋与湖沼》2015,46(5):1103-1108

为建立一种简单快捷的水产品中多氯联苯(PCBs)检测方法,本研究应用电子鼻对青蛤(Cyclina sinensis)软组织中富集的多氯联苯进行检测,并采用HS-SPME-GC-MS(顶空固相微萃取-气质联用)对结果进行了验证。首先利用电子鼻制作PCB15浓度梯度模版,检测PCB15处理72h以及144h的青蛤软组织中残留量,采用LDA和PCA两种方法统计并同模板的浓度进行比较。结论:青蛤软组织中PCB15浓度能明显分开,并随着处理时间的延长,区分效果愈加明显,区分度愈大。PCA的分辨效果明显优于LDA。运用欧氏距离、马氏距离、判别函数法和相关性对含有PCB15的样品浓度分别进行鉴别和验证,四种方法总的鉴别率均达到90%以上,能够较准确地对样品进行验证。电子鼻检测青蛤软组织中的多氯联苯的最低检测限为0.05μg/L,GC-MS为0.5μg/L。  相似文献   

粤西沿海一次赤潮事件的三维观测     

谢玲玲  陈清香  胡建宇  张书文  伊小飞  陈法锦  邓锐  邓晓东  王静  齐义泉 《海洋学报(英文版)》2015,34(1):159-161

From November 24 to 26, 2014, a red tide event occurred in the offshore water off the Hailing Island located at the western Guangdong coast. The red tide appeared as pink strips distributed within 3 km in the offshore water and extended for about 10 km along the shoreline. During the flood tide, the pink seawater rushed to the beach with breaking waves, forming foam strips on the beach. Guangdong Province Key Laboratory for Coastal Ocean Variation and Disaster Prediction Technologies, Guangdong Ocean University, emergently responded to the event and organized three-dimensional observations from the air, onboard and on beach. The preliminary analyses of the cruise data and water samples indicate that the event was induced by nontoxic Noctiluca scintillans, of which the concentration reaches as high as 4 200 cells/L near the surface and 2 600 cells/L at the bottom.  相似文献   

在大洋环流模型中对应于惯性内波破碎的垂直混合的一个参数化方案   总被引：1，自引：0，他引：1  

范植松  尚真琦  张善武  胡瑞金  刘海龙 《海洋学报(英文版)》2015,34(1):11-22

Based on the theoretical spectral model of inertial internal wave breaking(fine structure) proposed previously, in which the effects of the horizontal Coriolis frequency component f-tilde on a potential isopycnal are taken into account, a parameterization scheme of vertical mixing in the stably stratified interior below the surface mixed layer in the ocean general circulation model(OGCM) is put forward preliminarily in this paper. Besides turbulence, the impact of sub-mesoscale oceanic processes(including inertial internal wave breaking product) on oceanic interior mixing is emphasized. We suggest that adding the inertial internal wave breaking mixing scheme(F-scheme for short) put forward in this paper to the turbulence mixing scheme of Canuto et al.( T-scheme for short) in the OGCM, except the region from 15°S to 15°N. The numerical results of F-scheme by using WOA09 data and an OGCM(LICOM, LASG/IAP climate system ocean model) over the global ocean are given. A notable improvement in the simulation of salinity and temperature over the global ocean is attained by using T-scheme adding F-scheme, especially in the mid- and high-latitude regions in the simulation of the intermediate water and deep water. We conjecture that the inertial internal wave breaking mixing and inertial forcing of wind might be one of important mechanisms maintaining the ventilation process. The modeling strength of the Atlantic meridional overturning circulation(AMOC) by using T-scheme adding F-scheme may be more reasonable than that by using T-scheme alone, though the physical processes need to be further studied, and the overflow parameterization needs to be incorporated. A shortcoming in F-scheme is that in this paper the error of simulated salinity and temperature by using T-scheme adding F-scheme is larger than that by using T-scheme alone in the subsurface layer.  相似文献   

南海北部陆架区两个台风过境时近惯性运动的若干特征   总被引：2，自引：1，他引：1  

CHEN Shengli  HU Jianyu  POLTON Jeff A. 《海洋学报(英文版)》2015,34(1):38-43

Features of near-inertial motions on the shelf(60 m deep) of the northern South China Sea were observed under the passage of two typhoons during the summer of 2009. There are two peaks in spectra at both sub-inertial and super-inertial frequencies. The super-inertial energy maximizes near the surface, while the sub-inertial energy maximizes at a deeper layer of 15 m. The sub-inertial shift of frequency is induced by the negative background vorticity. The super-inertial shift is probably attributed to the near-inertial wave propagating from higher latitudes. The near-inertial currents exhibit a two-layer pattern being separated at mid-depth(25–30 m), with the phase in the upper layer being nearly opposite to that in the lower layer. The vertical propagation of phase implies that the near-inertial energy is not dominantly downward. The upward flux of the near-inertial energy is more evident at the surface layer(17 m). There exist two boundaries at 17 and 40 m, where the near-inertial energy is reflected upward and downward. The near-inertial motion is intermittent and can reach a peak of as much as 30 cm/s. The passage of Typhoon Nangka generates an intensive near-inertial event, but Typhoon Linfa does not. This difference is attributed to the relative mooring locations, which is on the right hand side of Nangka's path(leading to a wind pattern rotating clockwise with time) and is on the left hand side of Linfa's path(leading to a wind pattern rotating anti-clockwise with time).  相似文献   

山东半岛北部滨浅海区表层沉积物铝物源鉴别     

徐刚  刘健  裴绍峰  孔祥淮  胡刚  高茂生 《海洋学报(英文版)》2015,34(12):147-153

Surface sediment samples in the near shore area of the north Shandong Peninsula are collected for grain size and element analyses. The results indicate that the surface sediments in the study area are primarily composed of the silt-sized components similar to the Huanghe River. The total concentration of aluminum varies from 5.57% to7.37%(average(6.33 ± 0.40)%), and its spatial distribution is mainly controlled by the grain size. Correlations between the ratio of aluminum to titanium concentration and aluminum concentration, titanium concentration and the mean grain size indicate that aluminum in the near shore surface sediments is affected majorly by the terrigenous source, and partially by the anthropogenic source. The ratios of aluminum to titanium concentrations are larger than the background value of loess matter at some stations due to the existence of excess aluminum associated with human activities. Thus, the sources of aluminum should be identified firstly when aluminum is used as an index of terrigenous matter even in the near shore area dominated by terrigenous deposits.  相似文献   

中国南海一株固氮类芽孢杆菌的筛选和分离鉴定   总被引：1，自引：0，他引：1  

张丽红  刘艳芳  胡青平 《海洋科学》2015,39(3):100-105

为了解中国南海海洋自生固氮菌的种类,作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理,无氮培养基平板初筛后,对分离获得的细菌固氮酶结构基因nif H进行扩增,并对其固氮酶活性进行检测,最终获得一株能够产芽孢的固氮细菌。对该菌株进行生理生化性状测定、16S r DNA序列分析(Gen Bank登录号KJ627376),并基于nif H、16S r DNA系统进化树分析,确定该菌为一株固氮类芽孢菌(Paenibacillus sp.)NH-1。本研究表明固氮类芽孢杆菌在海洋中确有分布,海洋自生固氮菌的多样性远远超出人们之前的认识。  相似文献   

斑马鱼GSTM单核苷酸多态性与低温耐受性的相关分析     

王倩  尤锋  辛梦娇  胡金伟  谭训刚 《海洋科学》2015,39(1):1-7

为分析谷胱甘肽硫-转移酶M基因(glutathione S-transferases M,GSTM)与鱼类低温耐受性的相关性,本实验运用PCR-SSCP技术研究了130尾斑马鱼(Danio rerio)GSTM基因5?UTR、3?UTR和第一内含子序列的单核苷酸多态性(Single nucleotide polymorphisms,SNPs),同时分析了筛选到的基因型与其低温耐受性状的关联性。结果显示,在其5UTR区域检测到AB、BC、AC 3种基因型个体,共A、B、C 3个等位基因,其观测杂合度和期望杂合度分别是1.000和0.570,多态信息含量为0.472,所检测群体在该座位偏离了Hardy-Weinberg平衡;第1内含子中检测到DD、DE和EE 3种基因型个体,共D、E两个等位基因,其观测杂合度和期望杂合度分别为0.408和0.477,多态信息含量为0.362,群体在该座位符合Hardy-Weinberg平衡。3'UTR区域中没有发现多态性。上述2个SNP座位与斑马鱼低温耐受性状的关联分析结果表明,5'UTR区3种基因型与低温耐受性状均没有显著相关性(χ2=4.029,P0.05)。而第1内含子3种基因型与低温耐受性状显著相关(χ2=8.498,P0.05):DD基因型在耐低温群体中占优势(50.00%),并表现为对受低温胁迫斑马鱼的保护性因素(OR=0.520,95%CI=0.255–1.061),而DE基因型在不耐低温群体中占优势(51.31%),表现为低温胁迫下斑马鱼的危险因素(OR=3.012,95%CI=1.413–6.419)。研究结果为GSTM基因SNPs位点与斑马鱼低温耐受性能关联分析提供了依据,也将为海水经济鱼类抗寒标记筛选育种提供参考。  相似文献   

地图审校和错误修改的定义及其应用          下载免费PDF全文

钟业勋  胡宝清  边少锋  李厚朴 《海洋测绘》2015,(2):26-28

地物的性质和位置是地图表示的两个基本要素。制图者对地物的反映,即是制图者关于地物的观念模型和地图符号的超前表象。根据观念模型与地图符号的距离函数,定义地图符号的正确或错误。发现地图符号的错误并标注出来是地图审校的本质特征。地图符号的修改定义为地图符号从错误到正确的变换。地图审校和错误修改是传统和现代地图制图中确保地图质量的通用模式。  相似文献   

张扭性断陷盆地构造样式与油气成藏的关系——以珠江口盆地恩平凹陷新生界为例   总被引：1，自引：0，他引：1  

许新明  刘贤来  陈胜红  王绪诚  胡坤 《海洋地质前沿》2015,31(1)

从张扭性断陷盆地的断裂特征入手,结合构造动力学形成机制,总结了恩平凹陷的构造样式特征及有利圈闭类型,并探讨了构造样式对烃源岩的形成和油气富集区带的控制作用。结果表明:恩平凹陷为双向伸展的上下构造层变形系统的叠合,即古近系为NE—EW向伸展构造系统,新近系为NW—NWW向伸展—张性扭动构造系统;发育了4大类伸展型构造样式,构造样式演化控制了半地堑结构、烃源岩发育情况、油气的初始运移方向和断裂构造带与圈闭类型分布,为新生界油气藏的形成创造了有利条件。  相似文献   

海水升温对大亚湾浮游植物群落结构和光合活性的影响   总被引：1，自引：0，他引：1       下载免费PDF全文

谢艳辉  李涛  简伟军  胡思敏  田媛  刘胜 《热带海洋学报》2015,34(2):24-31

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