Purification and characterization of an alkaline protease from Acetes chinensis     

Xu Jiachao  Liu Xin  Li Zhaojie  Xu Jie  Xue Changhu  Gao Xin 《中国海洋大学学报(英文版)》2005,4(3):257-261

An alkaline protease from Acetes chinensis was purified and characterized in this study. The steps of purification include ammonium sulfate precipitation, ion-exchange chromatography with Q-sepharose Fast Flow, gel filtration chromatography with S300 and the second ion-exchange chromatography with Q-sepharose Fast Flow. The protease was isolated and purified, which was present and active on protein substrates (azocasein and casein). The specific protease activity was 17.15 folds and the recovery was 4.67. The molecular weight of the protease was estimated at 23.2 kD by SDS-PAGE. With azocasein as the susbstrate, the optimal temperature was 55°C and the optimal pH value was 5.5. Ion Ca²⁺ could enhance the proteolytic activity of the protease, while Cu²⁺, EDTA and PMSF could inhibit its activity.  相似文献   

类氖铁(Fe XVII)离子能级及振子强度计算     

仲佳勇  张杰  赵刚  鲁欣 《天文学报》2004,45(1):16-24

利用原子数据模拟程序RCN／RCG、AUTOSTRUCTURE(AS)、GRASP分别对类氖铁的离子结构进行计算，计算过程包含了对波函数的相对论修正，计算结果包括电偶级光谱跃迁的能级间隔，谐振子强度，跃迁几率，并与实验值进行了比较，分析了3种原子数据模拟程序在计算原子结构上的精确度．  相似文献   

沂沭断裂带垂直形变与断层活动特征分析   总被引：8，自引：8，他引：0  

李杰  韩海华  闫德桥  刘敏 《大地测量与地球动力学》2002,22(2):106-111

利用跨断层短水准资料分析了沂沭断裂带断层活动特征，结果表明：NNE向的沂沭断裂带与NW向断裂活动性质有所不同，沂沭断裂带主要为压性逆断活动，北西向断裂带为张性正断活动，与地质考察结果基本一致。此外，沂沭断裂带及北西向断裂带都具有分段活动的特点。  相似文献   

煤矿地区国土总体规划的初步研究   总被引：3，自引：0，他引：3  

凡杰 《地理研究》1988,7(1):46-52

本文通过制定总体规划方案应重点协调的六个关系的探讨,对煤矿地区国上总体规划进行了初步研究。  相似文献   

The Data Collection and Process System for RDT-10000 Type Rock High Pressure Dynamic Triaxial Machine     

Chang Jie 《岩土力学》1989,10(2):83-87

A data collection and process system is developed for RDT-10000 Type Rock Dynamic Triaxial Machine with High pressure, this system is supported by several subroutines in 6502 assembly language, which is of high-speed, flexibility, multi-functions.  相似文献   

Influencing domain of peripheral sources in the urban heavy pollution process of Beijing   总被引：1，自引：0，他引：1  

KANG Shichang  Paul A. Mayewski 《中国科学D辑(英文版)》2005,48(4):565-575

Multiple cities in a diveloped economic area may consist of a city cluster,and the difusion and mixing of its pollutants result in the effect of pollutants plume between cities and the large-scale regional pollution diffusion phenomenon.The distant transfer and diffusion of pollutants occurs when massive aerosols are affected by the dynamic porcess of large-scale circulations.Research suggesten that the life span of aerosol particles whose diameters are about 1 um is the longest.The longevity …  相似文献   

INFLUENCE OF THE SURFACE AIR TEMPERATURE OVER ASIAN-PACIFIC REGION ON THE SUMMERTIME NORTHEASTERN ASIAN BLOCKING HIGH^*          下载免费PDF全文

CAO Jie  DONG Huilin  YAO Ping 《Acta Meteorologica Sinica》2004,18(4):455-466

Synthesis analysis and singular value decomposition (SVD) methods were used to study the impact of surface air temperature (SAT) over Asian-Pacific region on the summertime northeastern Asian blocking high (NABH) with NCEP/NCAR Reanalysis Data.The results showed that 500 hPa geopotential height and SAT fields over Asian-Pacific region shared the similar pattern of East Asian Pacific (EAP) wave train;there was steady remote response relationship between the EAP wave train in summer and the "+-+" pattern of tropical SAT in zonal direction from former winter to summer;there were two relative negative(positive) Walker circulations over the tropical Indian Ocean and Pacific when being more(less) summertime NABH. The influence of sea surface temperature anomaly (SSTA) on the summertime NABH was possibly as follows.The special distribution of SSTA in tropical zonal direction continuously forced the tropical convection and zonal circulation from former winter to summer,and led them to act anomaly.Finally the abnormal conditions were transported to middle-high latitudes through EAP wave train and yielded the advantageous or disadvantageous atmospheric circulation background for the summertime NABH.  相似文献   

抗差估计中几种选权迭代法常数选取的探讨   总被引：9，自引：0，他引：9  

李浩军  唐诗华  黄杰 《测绘科学》2006,31(6):70-71,76

常规抗差估计中采用选权迭代法时常数的取值是否合理直接关系到平差过程能否顺利进行和平差结果的优劣,本文通过对目前几种选权迭代法中常数的选取进行研究;得出:常数按K1=m ax(|(v1-l1)|)、C=m ax(|(vi-li)|)取值时,能有效解决常规取值方法(K1=3.0~8.0或K1=3.0~6.0,C=8)在粗差较大时出现秩亏、法方程系数矩阵奇异的现象,导致平差不能进行或者平差结果出现严重扭曲的问题,同时本文论述了在确保有解的情况下得到最优解的处理方法。  相似文献   

起始方位角误差对附合导线平差结果的影响   总被引：1，自引：0，他引：1  

张景湘  马捷  赵龙平 《测绘科学技术学报》2006,23(1):62-64,68

附合导线是工程测量中最常见的布网形式.结合附合导线平差过程,文中用真误差分析方法推导了起始方位角误差对附合导线平差结果的影响公式;通过分析与数据计算,得到了起始方位角误差对平差后导线点坐标和导线边方位角的影响规律;并与测角误差影响进行比较,得出了起始方位角误差影响可忽略的条件,对导线测量的理论研究与生产实践具有参考意义.  相似文献   

基于知识的LIDAR数据地物提取研究   总被引：1，自引：0，他引：1  

杨海全  余洁  秦昆  张国宁 《测绘通报》2006,(12):9-11,15

激光雷达能够直接获取所观测对象表面点的3维坐标以及该点的强度信息。在缺少光谱信息以及其他的辅助数据的情况下,如何有效地从激光扫描所获取的“点云”数据中进行地物点的分类和提取,是目前LIDAR数据处理研究的重点问题之一。提出一种在缺乏其他辅助数据的情况下,基于知识的辅助,直接从激光雷达扫描数据中进行地物提取的方法。实验证明该方法能有效地从LIDAR数据中提取出感兴趣的目标点。  相似文献