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21.
Expression of intracellular ammonium assimilation enzymes were used to assess the response of nitrogen (N) metabolism in bacterioplankton to N-loading of sub-tropical coastal waters of Key West, Florida. Specific activities of glutamine synthetase (GS) and total glutamate dehydrogenase (GDHT) were measured on the bacterial size fraction (<0.8 μm) to assess N-deplete versus N-replete metabolic states, respectively. Enzyme results were compared to concentrations of dissolved organic matter and nutrients and to the biomass and production of phytoplankton and bacteria. Concentrations of dissolved inorganic N (DIN), dissolved organic N (DON), and dissolved organic carbon (DOC) positively correlated with specific activities of GDHT and negatively correlated with that of GS. Total dissolved N (TDN) concentration explained 81% of variance in bacterioplankton GDHT:GS activity ratio. The GDHT:GS ratio, TDN, DOC, and bacterial parameters decreased in magnitude along a tidally dynamic trophic gradient from north of Key West to south at the reef tract, which is consistent with the combined effects of localized coastal eutrophication and tidal exchange of seawater from the Southwest Florida Shelf and Florida Strait. The N-replete bacterioplankton north of Key West can regenerate ammonium which sustains primary production transported south to the reef. The range in GDHT:GS ratios was 5–30 times greater than that for commonly used indicators of planktonic eutrophication, which emphasizes the sensitivity of bacterioplankton N-metabolism to changes in N-bioavailability caused by nutrient pollution in sub-tropical coastal waters and utility of GDHT:GS ratio as an bioindicator of N-replete conditions. 相似文献
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德尔尼钴铜矿床主要矿石为致密块状含铜黄铁矿,上世纪60年代通过在矿区和外围投入11∶0000面积性自电、磁法测网,圈定自电异常28处,其中确定为矿致异常的14处,自电方法在德尔尼铜钴矿勘探初期发挥了重要作用。但利用这种找矿模式,从上世纪70年代至今,矿区外围找矿尚无突破性进展,这就需要对勘查方法进行比较与优选。本文通过对前人在矿区进行的物探方法加以比较,设计地电模型,进行瞬变电磁方法模拟计算,分析用瞬变电磁法探测矿体的有效性并在已知矿区进行的瞬变电磁剖面试验中获得成功,试验方法效果好,可以用于进一步外围找矿。 相似文献
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The 3150-2700 cm-1 (3.17-3.70 microns) range of the spectra of a number of Ar-matrix-isolated PAHs containing excess H atoms (Hn-PAHs) are presented. This region covers features produced by aromatic and aliphatic C-H stretching vibrations as well as overtone and combination bands involving lower lying fundamentals. The aliphatic C-H stretches in molecules of this type having low to modest excess H coverage provide excellent fits to a number of the weak emission features superposed on the plateau between 3080 and 2700 cm-1 (3.25 and 3.7 microns) in the spectra of many planetary nebulae, reflection nebulae, and H II regions. Higher H coverage is implied for a few objects. We compare these results in context with the other suggested identifications of the emission features in the 2950-2700 cm-1 (3.39-3.70 microns) region and briefly discuss their astrophysical implications. 相似文献
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青海祁漫塔格地区野马泉花岗闪长岩LA-ICP-MS锆石U-Pb年龄及其地质意义 总被引:1,自引:1,他引:0
青海东昆仑复合造山带西段的祁漫塔格地区是一个特征显著的侵入岩浆构造带,同时也是重要的多金属成矿带。野马泉铁铜多金属矿是该区较为典型的接触交代矽卡岩型矿床。利用LA-ICP-MS锆石U-Pb定年方法,获得该矿区与野马泉铁铜矿床有关的花岗闪长岩体的成岩年龄为226±2Ma;该结果与前人利用辉钼矿Re-Os法获得的矽卡岩型铜钼多金属矿石和矽卡岩型钼矿石的等时线年龄一致,因此本文厘定野马泉矿区成岩成矿时代为中—晚三叠世。 相似文献
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Marigómez I Soto M Cancio I Orbea A Garmendia L Cajaraville MP 《Marine pollution bulletin》2006,53(5-7):287-304
In order to assess the biological effects of the Prestige oil spill (POS), mussels (Mytilus galloprovincialis), European hake (Merluccius merluccius) and European anchovy (Engraulis encrasicolus) were sampled between April and September 2003 in various geographical areas of Bay of Biscay: Galicia, Central Cantabrian and East Cantabrian. In mussels, several cell and tissue biomarkers were measured: peroxisome proliferation as induction of acyl-CoA oxidase (AOX) activity, lysosomal responses as changes in the structure (lysosomal volume density, V(V(L)), surface-to-volume ratio, S/V(L), and numerical density, N(V(L))) and in membrane stability (labilization period, LP), cell type replacement as relative proportion of basophilic cells (volume density of basophilic cells, V(V(BAS))) in digestive gland epithelium, and changes in the morphology of digestive alveoli as mean luminal radius to mean epithelial thickness (MLR/MET). Additionally, flesh condition index (FCI) and gonad index (GI) were measured as supporting parameters. In hake and anchovy, liver histopathology was examined to determine the prevalence of parasites, melanomacrophage centers, non-specific lesions (inflammatory changes, atrophy, necrosis, apoptosis), early non-neoplastic toxicopathic lesions (i.e., hepatocellular nuclear polymorphism), foci of cellular alteration, benign and malignant neoplasms. In mussels, AOX induction was noticeable in April except in Galicia. LP values were low in all the geographical areas studied, indicating disturbed health, especially in Galicia. Alike, lysosomal enlargement was observed in most stations as shown by the extremely low S/V(L) values. V(V(BAS)) and MLR/MET values were markedly high. Overall, employed biomarkers detected exposure to toxic chemicals and disturbed health in mussels from Bay of Biscay, with impact decreasing from April to September. Although hepatocellular nuclear polymorphism and nematode parasitization in fish liver were remarkably prominent in some areas, they cannot be hitherto related to POS, since we lack historical data to determine whether the prevalences found were normal or significantly raised after POS. 相似文献
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