分光光度法测定海洋河弧菌510脂多糖     

张运涛  顾谦群  方玉春  吕元萌  管华诗 《中国海洋大学学报(自然科学版)》2003,33(3):355-360

本文研究了直接测定海洋河弧菌 (vibrio fluvailis) 5 10脂多糖的分光光度法。利用 1,9-二甲基亚甲蓝 (DMB) ,在适当条件下同脂多糖发生染色反应 ,在 5 5 0 nm波长下 ,反应体系的吸光值同脂多糖含量呈线性相关。相关系数为 0 .9993,最低检出限为 10 μg/m L,相对标准偏差为 1.6 2 ,平均回收率为 (10 5± 2 .18) %。  相似文献   

大菱鲆幼鱼视网膜结构及不同光谱下视蛋白基因表达特征的研究          下载免费PDF全文

刘峰  吴乐乐  王雨浓  齐婷  徐世宏  李军  李贤 《海洋科学》2023,47(12):30-39

大菱鲆(Scophthalmus maximus)是中国重要的养殖鱼类之一, 其生活史经历不同的栖息地和光环境, 视觉器官结构及视觉功能也具有适应性发育特征和可塑性。本研究以不同发育阶段的大菱鲆幼鱼为对象, 探究了其视网膜结构变化、视蛋白基因的表达特征及其与光谱之间的关系。结果表明, 随着大菱鲆幼鱼的生长发育, 其视网膜的外核层逐渐变厚; 视锥视杆层的厚度变化不明显; 而内核层与神经节细胞层逐渐变薄。视紫红质基因rh1与视蛋白基因总表达量的比例上升, 由2月龄的57.35%上升到9月龄77.19%; 视锥蛋白基因与视蛋白基因总表达量的比例下降; 其中红视蛋白基因lws 由2月龄的4.49%下降至9月龄的0.13%。将7月龄的大菱鲆幼鱼用不同光谱处理75 d后, 其视蛋白基因的表达会随光谱环境的变化而发生改变。与全光谱相比, rh2b1、sws2、sws1在红、黄光下的表达量显著下降, 而rh2b1在蓝、绿光下表达显著上升、sws2在绿光下表达显著上升, 其他则变化不显著(P<0.05)。黄、绿、蓝及全光谱下rh2基因家族与视蛋白基因总表达量的比例最高, 而红光下基因rh1的表达量占比最高。大菱鲆幼鱼在不同阶段以及不同光谱处理下表现出了视蛋白基因表达的可塑性以适应不同水层的光谱环境。本研究为探究大菱鲆对光环境的适应机制及工厂化养殖光照调控技术的建立提供理论参考。  相似文献   

A combined numerical tidal model for the Hangzhou Bay and Qiantang River   总被引：2，自引：0，他引：2  

Cao Deming  Fang Guohong 《海洋学报(英文版)》1989,8(4):485-496

-In order to avoid prescribing open boundary condition on the upstream side of the Hangzhou Bay, in numerical simulation of the tides and residual currents of the Bay, a 1-D model for the Qiantang River is connected to the 2-D model for the Hangzhou Bay. The harmonic constants of diurnal constituent [ (K1 O1)/2],semidiurnal constituent (M2) and shallow water constituent (M4) are obtained. The results produced by the combined model are in better agreement with the observed ones than those produced solely by the original 2-D model. The combined model gives much more reliable results for tide-induced residual water level and current.  相似文献   

鲢鱼(Hypophthalmichthys molitrix)摄食强度和节律的实验研究     

王芳  董双林  李德尚  邴旭文 《中国海洋大学学报(自然科学版)》1993,(2)

报道用室内实验方法对鲢鱼摄食强度和摄食节律的研究结果,探讨了影响它们的因子。提出鲢鱼在不同水温、不同花粉密度及不同鱼规格下的摄食量的统计分析。指出:食粒密度和水温与摄食量正相关,而鱼规格与摄食量负相关;食粒密度对摄食量影响最大(P<0.01),其次为水温;水温和食粒密度在影响摄食量的过程中可能有一定的交互作用。鲢鱼摄食强度在室内条件下也具明显的昼夜节律。在食粒规格较小、密度较低且溶氧充足条件下,鲢鱼夜晚非但不停食且出现摄食率次高峰。在本实验条件下,鲢鱼的日摄食节律与水温和光照无关,可能受饵料可得性和自身的生物钟节律等制约。  相似文献   

On the long-term joint distribution of characteristic wave height and period and its application     

Fang Zhongsheng  Dai Shunsun  Jin Chengyi 《海洋学报(英文版)》1989,8(3):315-325

By analysing the scatter diagrams of characteristic the wave height H and the period T on the basis of instrumental data from various ocean wave stations, we found that the conditional expectation and standard deviation of wave period for a given wave height can be better predicted by using the equations of normal linear regression rather than by those based on the log- normal law. The latter was implied in Ochi' s bivariate log-normal model(Ochi. 1978) for the long-term joint distribution of H and T. With the expectation and standard deviation predicted by the normal linear regression equations and applying proper types of distribution, we have obtained the conditional distribution of T for given H. Then combining this conditional P(T / H) with long-term marginal distribution of the wave height P(H) we establish a new parameterized model for the long-term joint distribution P(H,T). As an example of the application of the new model we give a method for estimating wave period associated with an extreme w  相似文献   

渤海三维海洋温度和海流数值预报   总被引：3，自引：2，他引：3  

刘钦政  张建华  刘煜  王旭  李燕  白珊  吴辉碇 《海洋预报》2005,22(Z1):27-34

"十·五"期间,我国开展了三维海洋温度和海流数值预报的业务化研究工作.经过3年的努力,渤海三维海洋温度和海流数值预报系统研制完成,并于2003年10月,开始试预报.本文对该预报系统以及运行情况进行了介绍,并分析了所存在的问题和发展方向.  相似文献   

E1 Nio phenomenon in simple ocean data assimilation data     

GUI Maochang  *  WU Lingjuan  . Institute of Oceanology  Chinese Academy of Sciences  Qingdao  China. Laboratory of Numerical Modeling for Atmospheric Sciences  Geophysical Fluid Dynamics  Institute of Atmospheric Physics  Chine-se Academy of Sciences  Beijing  China. Graduate School  Chinese Academy of Sciences  Beijing  China 《海洋学报(英文版)》2005,(4)

1 IntroductionIt is well known that interaction between the trop-ical ocean and atmosphere produces the largest inter-annual climate signal, El Nino-Southern Oscillation(ENSO). In past decades many efforts have been madein understanding and predicting ENSO: such as the hy-pothesis of Bjerknes (1969) that ENSO arises as aself-sustained cycle in which SSTA in the Pacific O-cean causes the trade winds to strengthen or slackenand that this in turn drives the ocean circulation changesthat …  相似文献   

Long-Term Effect of TMD on Vibration Control of An MDOF Offshore Fixed Platform     

SHI  Xiang 《中国海洋工程》2003,17(4):481-494

A three-dimensional fixed offshore platform in deep water modeled by the finite element method is studied in this paper. Analysis of the dynamic response of the MDOF structure is realized taking the non-linearity of the wave drag force and the wave-structure interaction into account. The structural response statistics, which have Gaussian distributions, are used to evaluate the vibration effect of the structure without TMD and with TMD. And an optimal method to design TMD controlling the first mode of the multi-mode structure is proposed. Moreover, the probabilities of occurrence of sea states at the platform site are considered for prediction of the long-term effect of a TMD. Simulation results demonstrate that the long-term effect of a well-designed TMD is good and the practical use is possible due to the good stability of its optimal parameters under different sea states.  相似文献   

西洋东大港水道流速垂线分布研究   总被引：2，自引：0，他引：2  

吴德安  张忍顺 《海洋工程》2005,23(1):88-92,96

对辐射沙洲海域东大港4#站位连续2个潮周期的流速测量资料进行了分析、研究,拟合给出了流速垂线分布关系,并根据实际测量资料确定了该站位6个水层的浑水水流尾流函数项表达式。这些表达式表明浑水水流尾流函数是相对深度和摩阻速度的函数。探讨分析了尾流函数的空间分布和潮周期过程。非恒定流的水流尾流函数项的准确表达,对于揭示含沙水流流速结构及水、沙相影响作用规律将起到启发和推动作用。  相似文献   

河口涨落潮槽水动力特征及河槽类型判定   总被引：4，自引：0，他引：4  

刘高峰  沈焕庭  吴加学  吴华林 《海洋学报》2005,27(5):151-156

在前人对河口冲刷槽研究的基础上,选择长江口南支、新桥水道、南小泓和南港主槽作为典型涨、落潮槽研究对象,以2001年洪季和2003年枯季实测资料为根据,比较分析了长江口涨落潮槽的水流、泥沙、优势流和优势沙等动力特征,结果表明用单一因子分析河槽性质不够全面.提出了一个(无量纲因子)河槽类型系数(λ)来综合表述河槽的多种水文泥沙特征,可以较全面地用多个影响因子来合理地判断河槽类型.  相似文献