大口黑鲈抗菌肽hepcidin cDNA序列和结构分析   总被引：2，自引：0，他引：2  

白俊杰  刘碧莲  劳海华  叶星  简清 《广东海洋大学学报》2008,28(1):1-5

以大口黑鲈为材料,提取肝脏总RNA,经RT-PCR扩增出hepcidin cDNA的开放阅读框(ORF)及3′端非编码区序列,应用5′RACE方法得到大口黑鲈hepcidin cDNA5′末端。将所获得的两个片段分别克隆到T载体后进行测序,并拼接成大口黑鲈hepcidin全长cDNA。序列分析表明:大口黑鲈hepcidin全长cDNA为564bp,含有一个258bp的ORF,编码86个氨基酸残基,由信号肽(24个残基)、前肽(42个残基)和成熟肽(20个残基)3部分组成hepcidin前体。在前肽部分具有前肽转化酶典型的RX(K/R)R基元,成熟肽部分含有8个保守的半胱氨酸残基,可形成四个链内二硫桥,使β-折叠结构保持稳定。大口黑鲈Hepcidin与其他鱼类的同源性在29.7%~90.5%间,尤其是信号肽区域,与鳜、尼罗罗非鱼、真鲷、花鲈、黑鯛、金眼狼鲈仅有2~3个氨基酸的差别。  相似文献   

南黄海前新生代地震采集技术研究     

吴志强  许行  童思友  刘怀山  张一波 《海洋地质前沿》2008,24(8):20-25

根据南黄海区域地质特征、南黄海海相中一古生代碳酸盐岩地层构造与地球物理特征,在分析了前期的地震成果和经验的基础上,进行了采集参数的研究,设计了适用于前新生代地震勘探目标层的地震采集参数,进行了地震资料采集,取得了品质良好的地震资料。  相似文献   

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玄松柏  邢乐林  何志堂  张世伟  李辉 《大地测量与地球动力学》2008,28(4):72-74

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Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)     

Shouming Feng  Wenbin Zhan  Jing Xing  Jun Li  Kai Yang  Jing Wang 《中国海洋大学学报(英文版)》2008,7(3):287-293

The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.  相似文献   

Composition of plagioclases in volcanic rocks of King George Island, Antarctica with reference to the petrogenetic significance     

Xing Guangfu  Jin Qingmin 《极地研究(英文版)》2000,11(2)

Plagioclases occur mainly as phenocrysts in volcanic rocks of King George Island, South Shetland Islands, West Antarctica. In basaltic andesites and andesites of Keller Peninsula and Ullman Spur (Admiralty Bay), they are high structure state labradorite-andesines; and in high-A1 basalts and basaltic andesites of Barton and Weaver peninsulas (Maxwell Bay), they are high structure state bytownite-anorthites.∑REE, La/Yb ratios and δEu values of plagioclases from Admiralty Bay are higher than those from Maxwell Bay. All plagioclases have rather identical chondritenormalized transitional element distribution patterns, probably reflecting that crystal structure rather than composition of plagioclase controls their diversity. Compositions of plagioclases depend chiefly on those of their host rocks, compositional differences of plagioclases reveal that basaltic magmas in the Admiralty Bay area are more evolved than in the Maxwell Bay area.  相似文献   

二氧化氯控制欧洲鳗细菌性疾病     

何义进  邢华  熊家娟 《广东海洋大学学报》2000,20(1)

以二氧化氯作为一种新型的消毒剂和杀菌剂 ,以传统含氯制剂 (如漂白粉、二氯异氰尿酸钠、三氯异氰尿酸 )作为对照 ,比较其在治疗欧洲鳗细菌性疾病及改善养殖水质方面的显著特性 ,其作用活性与环境 (如 p H、温度、氨及有机物 )因子的关系。结果表明 ,二氧化氯 (Cl O2 )对爱德华氏菌、嗜水气单胞菌和柱状屈挠杆菌有很好的杀灭效果 ,能迅速治愈欧洲鳗的赤鳍病和肝肾病 ,且能有效改善养殖水体 ,水中生化需氧量比对照组下降 10 %～ 30 % ,化学耗氧量下降 5% ,溶氧量提高 8%～ 10 %。当二氧化氯体积分数为 8m L/m3时 ,其杀菌率均高达 90 %以上 ,为氯制剂的 1.8倍 ,且活性不受环境因子的影响  相似文献   

应用Hydrolight模式研究含油水体辐射传输特性的关键技术          下载免费PDF全文

黄妙芬  骆蔚健  刘杨  邢旭峰  庄炀 《海洋技术学报》2019,38(3):7-14

辐射传输模式Hydrolight在国际水色遥感研究领域已被广泛用于解决水体光学的各种问题,也是进行石油类污染水体辐射传输特性研究的有效模型。结合Hydrolight模式的机理、应用现状及含油水体的特点,提出了将该模式应用于光学特性复杂的石油污染水体辐射传输特性研究时需要解决的关键技术,包括测定石油污染水体吸收系数和散射系数、建立石油污染吸收和散射特性随波长和深度变化的参数化模型、确定油物质的吸附后悬浮颗粒物体散射函数模型等方面。依据2018年8月和2016年8月分别在辽宁大连港和辽宁盘锦辽河油田等区域测定的含油水体数据,讨论了这些关键技术的解决方案。  相似文献   

全新世以来珠江三角洲海鸥沙形成过程的地貌动力学分析     

莫文渊  韦惺  吴超羽 《热带海洋学报》2019,38(3):68-78

海鸥沙是珠江三角洲一个极富特色的沉积砂体。本文基于钻孔资料并结合长周期“动力-沉积-形态”模型, 从沉积学和地貌动力学角度对全新世以来海鸥沙的形成演变过程进行了探讨。海欧沙在全新世的沉积层序自下而上分别为河流相、河口湾浅海相和三角洲相。全新世海侵盛期以来, 虎门涨潮射流和东北—西南向涨落潮流是影响海鸥沙形成演变的主要动力。6000—2500a BP, 受东北—西南向涨落潮流的影响, 海鸥沙中北部地区一直处于冲刷无沉积状态, 由虎门涨潮射流带来的泥沙主要在海鸥沙南部沉积, 沉积速率约为0.67mm·a ^-1; 2500—1700a BP, 随着番禺平原的发育, 东北—西南向涨落潮流逐渐消弱, 海鸥沙进入一个快速沉积期, 平均沉积速率约为15mm·a ^-1, 沉积由两端向中间发展; 1700—600a BP, 随着沙湾水道的形成, 海鸥沙中部迅速发展, 至600a BP左右, 海鸥沙基本形成并出露水面。  相似文献   

地理省情监测成果地图集编制探讨     

张文龙  潘星 《测绘》2019,(2):81-85

本文通过对《四川省地理省情监测成果地图集》在图集设计思路、地图编制方法等方面的探索,总结出了地理省情监测成果制图表达的基本流程,并以地图形式直观地展示地理省情监测成果。探索了地理国情监测成果图集的设计思路及方法,尤其是动态信息的制图表达方案,为开展地理国情、省情监测等综合地图图集的设计、编制提供借鉴。  相似文献   

Sex determination and differentiation in Aurelia sp.1: the absence of temperature dependence     

刘春胜  顾志峰  邢孟欣  孙云  陈四清  陈昭廷 《中国海洋湖沼学报》2018,36(2):457-464

Cnidarians, being regarded as ‘basal' metazoan animals, are considered to have relatively high plasticity in terms of sex reversal. In this study we used an experimental approach to demonstrate sexual diff erentiation and plasticity in benthic polyps and pelagic medusae of A urelia sp.1 maintained at dif ferent temperatures. Results indicated that in A urelia sp.1, sex diff erentiation has been determined at the polyp stage and that all medusae originating from a given polyp are, phenotypically, of the same sex. In addition, the sex of polyps budding from the same clone(either male or female) at dif ferent temperatures appears to be the same as that of the parent. The sex of medusae that had originated from a known-sex polyp was observed to remain the same as that of the parent, irrespective of differences in strobilation or rearing temperatures. These results indicate that the mechanism of sex determination of Aurelia sp.1. is not influenced by prevailing temperature regimes. A comparison of variability in terms of sexual plasticity of A urelia sp.1 with that of Hydrozoa and Anthozoa suggests that species characterized by a free-swimming medusa life stage have a high dispersal potential, which probably results in a lower rate of sex reversal.  相似文献