Evidence for multiple modes of uranium immobilization by an anaerobic bacterium     

Allison E. Ray  John R. Bargar  Alice C. Dohnalkova  Brent M. Peyton  Timothy S. Magnuson 《Geochimica et cosmochimica acta》2011,75(10):2684-6510

Microbial reduction of hexavalent uranium has been studied widely for its potential role in bioremediation and immobilization of soluble U(VI) in contaminated groundwater. More recently, some microorganisms have been examined for their role in immobilization of U(VI) via precipitation of uranyl phosphate minerals mediated by microbial phosphate release, alleviating the requirement for long-term redox control. Here, we investigated the mechanism of U(VI) removal mediated by an environmental isolate, strain UFO1, that is indigenous to the Field Research Center (FRC) in Oak Ridge, TN and has been detected in U(VI)-contaminated sediments. Changes in U(VI) speciation were examined in the presence and absence of the electron-shuttling moiety, anthraquinone-2,6-disulfonate (AQDS). Cell suspensions were capable of nearly complete removal of 100 μM U(VI) from solution within 48 h; U(VI) removal was not dependent on the presence of an exogenous electron donor or AQDS, although AQDS increased the rate of U(VI) removal. X-ray Absorption Near Edge Structure (XANES) and Extended X-ray Absorption Fine Structure (EXAFS) spectroscopic measurements indicated that U(IV) was the predominant oxidation state of uranium in cell suspensions in both the absence and presence of 100 μM AQDS. Interestingly, 17% of the cell-associated precipitates in a U(VI)-treated suspension that lacked AQDS had spectral characteristics consistent with a uranyl phosphate solid phase. The potential involvement of phosphate was consistent with observed increases in soluble phosphate concentrations over time in UFO1 cell suspensions, which suggested phosphate liberation from the cells. TEM-EDS confirmed the presence of uranyl phosphate with a U:P ratio consistent with autunite (1:1). EXAFS analyses further suggested that U(IV) was bound to low-Z neighbors such as C or P, inferred to be present as functional groups on biomass. These results suggest that strain UFO1 has the ability to facilitate U(VI) removal from solution via reductive and phosphate precipitation mechanisms. Both mechanisms offer potential for the remediation of U-contaminated sediments at the FRC or elsewhere.  相似文献   

The host galaxies and classification of active galactic nuclei     

Lisa J. Kewley †  Brent Groves  Guinevere Kauffmann  Tim Heckman 《Monthly notices of the Royal Astronomical Society》2006,372(3):961-976

  相似文献   

Ground‐based lidar measurements of aerosols during ACE‐2: instrument description, results, and comparisons with other ground‐based and airborne measurements     

Ellsworth J. Welton  Kenneth J. Voss  Howard R. Gordon  Hal Maring  Alexander Smirnov  Brent Holben  Beat Schmid  John M. Livingston  Philip B. Russell  Philip A. Durkee  Paola Formenti  Meinrat O. Andreae 《Tellus. Series B, Chemical and physical meteorology》2000,52(2):636-651

A micro‐pulse lidar system (MPL) was used to measure the vertical and horizontal distribution of aerosols during the Aerosol Characterization Experiment 2 (ACE‐2) in June and July of 1997. The MPL measurements were made at the Izaña observatory (IZO), a weather station located on a mountain ridge (28°18' N, 16°30' W, 2367 m asl) near the center of the island of Tenerife, Canary Islands. The MPL was used to acquire aerosol backscatter, extinction, and optical depth profiles for normal background periods and periods influenced by Saharan dust from North Africa. System tests and calibration procedures are discussed, and an analysis of aerosol optical profiles acquired during ACE‐2 is presented. MPL data taken during normal IZO conditions (no dust) showed that upslope aerosols appeared during the day and dissipated at night and that the layers were mostly confined to altitudes a few hundred meters above IZO. MPL data taken during a Saharan dust episode on 17 July showed that peak aerosol extinction values were an order of magnitude greater than molecular scattering over IZO, and that the dust layers extended to 5 km asl. The value of the dust backscatter–extinction ratio was determined to be 0.027±0.007 sr⁻¹. Comparisons of the MPL data with data from other co‐located instruments showed good agreement during the dust episode.  相似文献   

Stochastic injection-strategy optimization for the preliminary assessment of candidate geological storage sites     

Brent M. Cody  Domenico Baù  Ana González-Nicolás 《Hydrogeology Journal》2015,23(6):1229-1245

  相似文献   

Correlation and characterisation of individual glass shards from tephra deposits using trace element laser ablation ICP‐MS analyses: current status and future potential     

Nicholas J. G. Pearce  Joanna S. Denton  William T. Perkins  John A. Westgate  Brent V. Alloway 《第四纪科学杂志》2007,22(7):721-736

Laser ablation inductively coupled plasma mass spectrometry (LA‐ICP‐MS) is a high spatial resolution analytical method which has been applied to the analysis of silicic tephras. With current instrumentation, around 30 trace elements can be determined from single glass shards as small as ～ 40 µm, separated from tephra deposits. As a result of element fractionation during the ablation process using a 266 nm laser, a relatively complex calibration strategy is required. Nonetheless, such a strategy gives analyses which are accurate (typically within ±5%) and have an analytical precision which varies from ～ ±2% at 100 ppm, to ～ ±15% at 1 ppm. Detection limits for elements used in correlation and discrimination studies are well below 1 ppm. Examples of the application of trace element analysis by LA‐ICP‐MS in tephra studies are presented from the USA, New Zealand and the Mediterranean. Improvements in instrumental sensitivity in recent years have the potential to lower detection limits and improve analytical precision, thus allowing the analysis of smaller glass shards from more distal tephras. Laser systems operating at shorter wavelengths (e.g. 193 nm) are now more widely available, and produce a much more controllable ablation in glasses than 266 nm lasers. Crater sizes of <10 µm are easily achieved, and at 193 nm many of the elemental fractionation issues which mar longer wavelengths are overcome. By coupling a short wavelength laser to a modern ICP‐MS it should be possible to determine the trace element composition of glass shards as small as 20 µm and, providing sample preparation issues can be overcome, the determination of the more abundant trace elements in glass shards as small as 10 µm is within instrumental capabilities. This will make it possible to chemically fingerprint tephra deposits which are far from their sources, and will greatly extend the range over which geochemical correlation of tephras can be undertaken. Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

Classification of hydrological regimes of northern floodplain basins (Peace–Athabasca Delta,Canada) from analysis of stable isotopes (δ18O, δ2H) and water chemistry     

Brent B. Wolfe  Tammy L. Karst‐Riddoch  Roland I. Hall  Thomas W. D. Edwards  Michael C. English  Roger Palmini  Suzanne McGowan  Peter R. Leavitt  Sheila R. Vardy 《水文研究》2007,21(2):151-168

We used stable isotopes (δ¹⁸O and δ²H) and water chemistry to characterize the water balance and hydrolimnological relationships of 57 shallow aquatic basins in the Peace‐Athabasca Delta (PAD), northern Alberta, Canada, based on sampling at the end of the 2000 thaw season. Evaporation‐to‐inflow ratios (E/I) were estimated using an isotope mass‐balance model tailored to accommodate basin‐specific input water compositions, which provided an effective, first‐order, quantitative framework for identifying water balances and associated limnological characteristics spanning three main, previously identified drainage types. Open‐drainage basins (E/I < 0·4; n = 5), characterized by low alkalinity, low concentrations of nitrogen, dissolved organic carbon (DOC) and ions, and high minerogenic turbidity, include large, shallow basins that dominate the interior of the PAD and experience frequent or continuous river channel connection. Closed‐drainage basins (E/I ≥ 1·0; n = 16), in contrast, possess high alkalinity and high concentrations of nitrogen, DOC, and ions, and low minerogenic turbidity, and are located primarily in the relict and infrequently flooded landscape of the northern Peace sector of the delta. Several basins fall into the restricted‐drainage category (0·4 # E/I < 1·0; n = 26) with intermediate water chemistries and are predominant in the southern Athabasca sector, which is subject to active fluviodeltaic processes, including intermittent flooding from riverbank overflow. Integration of isotopic and limnological data also revealed evidence for a new fourth drainage type, mainly located near the large open‐drainage lakes that occupy the central portion of the delta but within the Athabasca sector (n = 10). These basins were very shallow (<50 cm deep) at the time of sampling and isotopically depleted, corresponding to E/I characteristic of restricted‐ and open‐drainage conditions. However, they are limnologically similar to closed‐drainage basins except for higher conductivity and higher concentrations of Ca²⁺ and Na⁺, and lower concentrations of SiO₂ and chlorophyll c. These distinct features are due to the overriding influence of recent summer rainfall on the basin water balance and chemistry. The close relationships evident between water balances and limnological conditions suggest that past and future changes in hydrology are likely to be coupled with marked alterations in water chemistry and, hence, the ecology of aquatic environments in the PAD. Copyright © 2006 John Wiley & Sons, Ltd.  相似文献   

Deglacial reservoir age and implications,Foxe Peninsula,Baffin Island     

Kayla J. Vickers  Brent C. Ward  Daniel J. Utting  Alice M. Telka 《第四纪科学杂志》2010,25(8):1338-1346

Marine reservoir age is reported for Foxe Basin, Canada, during deglaciation of the Laurentide Ice Sheet. Radiocarbon (¹⁴C) measurements were made on pairs of contemporaneous molluscs and Salix (willow) macrofossils, yielding a mean marine reservoir age of 985 ± 10 ¹⁴C a. Mean regional ΔR is calculated to be 615 ± 20 ¹⁴C a relative to a mean global reservoir age of ca. 400 ¹⁴C a. Previous studies in the Canadian Arctic that have been conducted on modern pre‐bomb molluscs give ΔR values of ca. 100–300 ¹⁴C a. The difference between modern and deglacial reservoir ages indicates that ΔR in Foxe Basin has changed over time, which has implications for the reconstruction of past events based on marine ¹⁴C dates. We recalculate the timing of deglaciation of Hudson Strait with this new reservoir age and relate this to the 8.2 cal. ka event. We recommend that local ΔR be determined for the time period being examined whenever possible, and that in cases where this is impossible a ΔR value of 615 ¹⁴C a be applied when calibrating marine samples from Foxe Basin and vicinity during deglaciation. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   

Partitioning Tracer Tests for Evaluating Remediation Performance   总被引：1，自引：0，他引：1  

R. Brent Cain  Gwynn R. Johnson  John E. McCray    William J. Blanford  Mark L. Brusseau 《Ground water》2000,38(5):752-761

  相似文献   

A network flow model for the genesis and migration of gas phase     

Koukung Alex Chang  W. Brent Lindquist 《Computational Geosciences》2013,17(1):67-81

We present a network flow model to compute transport, through a pore network, of a compositional fluid consisting of water with a dissolved hydrocarbon gas. The model captures single-phase flow (below local bubble point conditions) as well as the genesis and migration of the gas phase when bubble point conditions are achieved locally. Constant temperature computational tests were run on simulated 2D and 3D micro-networks near bubble point pressure conditions. In the 2D simulations which employed a homogeneous network, negligible capillary pressure, and linear relative permeability relations, the observed concentration of CO₂ dissolved in the liquid phase throughout the medium was linearly related to the liquid pressure. In the case of no gravity, the saturation of the gas phase throughout the medium was also linearly related to the liquid pressure; under gravity, the relationship became nonlinear in regions where buoyancy forces were significant. The 3D heterogeneous network model had nonnegligible capillary pressure and nonlinear relative permeability functions. While 100 % of the CO₂ entered the 3D network dissolved in the liquid phase, 25 % of the void space was occupied by gas phase and 47 % of the CO₂ exiting the outlet face did so via the gaseous phase after 500 s of simulation time.  相似文献   

Practical finite analytic methods for simulation of advection-dominated solute transport     

M. Salman Sabahi  Hanif Montazeri  Mojtaba Ardestani  Brent Sleep 《水文科学杂志》2013,58(12):2204-2213

Abstract

The practical finite-analytic (PFA) method was applied to the solution of the one-dimensional advection–dispersion equation (ADE) for solute transport in porous media under advection-dominated (high Peclet number, Pe) conditions. Several PFA spatial-temporal computational molecules were developed for Cauchy and pulse loading boundary conditions. The PFA solutions were compared with solutions from the upwind method and quadratic upwind differencing (QUICK) scheme. For all boundary conditions the trapezoidal explicit PFA (EPFA) computational molecule gave the most accurate results at very high Pe number as long as the Courant number (Cr) was close to one. Stability analysis shows that the PFA molecules are always stable for high Pe number.  相似文献