Preservation of enzymic activity in marine plankton by low-temperature freezing     

S.I. Ahmed  R.A. Kenner  F.D. King 《Marine Chemistry》1976,4(2):133-139

The measurement of enzymic activity in plankton communities is useful in the study of marine ecosystems. Such measurements can lead to a clearer understanding of the biological transformations in plankton communities at a particular time. However, the assays are somewhat time-consuming. To facilitate analysis of large numbers of samples, we have developed a method of quick-freezing of whole cells and of cell-free extracts of the diatom, Skeletonema costatum, followed by storage at ?60°C for up to one week. No loss in either the electron transport system (ETS) or the glutamate dehydrogenase (GDH) activity occurs. Similar conditions of storage can be used for the preservation of ETS activity in the marine copepod Calanus pacificus. Also, no measurable loss in either the GDH or the ETS activity is detectable after the quick-frozen whole cells of S. costatum have been kept frozen for over a year.  相似文献   

Coulometric total carbon dioxide analysis for marine studies: maximizing the performance of an automated gas extraction system and coulometric detector     

K.M. Johnson  K.D. Wills  D.B. Butler  W.K. Johnson  C.S. Wong 《Marine Chemistry》1993,44(2-4)

A global survey of the distribution of dissolved CO₂ taking advantage of sampling opportunities provided by the World Ocean Circulation Experiment: World Hydrographic Program (WOCE-WHP) is being carried out through 1995. Goals include the measurement of oceanic inorganic carbon transport and the development of a data base from which future fossil-fuel CO₂ build-up can be monitored. The analytical method selected for total carbon dioxide (C_T) is gas extraction of acidified seawater with coulometric titration of the acid formed by the resultant carbon dioxide and monoethanolamine. To combine high accuracy and precision (± 1.5 μmol/kg for C_T ≥ 2000 μmol/kg) with a high rate of analysis, we have modified an automated single-parameter system. Following prototype development between 1987 and 1990, an instrument emerged with the acronym Somma standing for single-operator multiparameter metabolic analyzer. Improved functional and operating procedures have integrated electronic calibration, CO₂ gas calibration, and sample analysis with automated pressure, temperature, and conductivity (salinity) sensing into a single convenient transportable package.  相似文献   

Errors in recent ocean tide models: possible origin and cause     

A. J. E. Smith  O. B. Andersen 《Progress in Oceanography》1997,40(1-4)

Recently, the TOPEX/POSEIDON Science Working Team has recommended the FES95.2.1 and CSR3.0 ocean tide models for reprocessing the TOPEX/POSEIDON Geophysical Data Records. Without doubt, the performance of these models, especially in the deep oceans, is excellent. However, from a comparison of these hydrodynamically consistent models with the purely empirical DW3.2 and DEOS96.1 models, it appears that FES95.2.1 and CSR3.0 are affected by basin boundary related errors which are caused by the basin-wise solution procedure of the FES ocean tide model series. In their turn, the empirical DW3.2 and DEOS96.1 models seem to suffer from significant errors in the Antarctic seas due to the seasonal growth and decay of Antarctic sea ice. Also, bathymetry-induced differences were found between the hydrodynamically consistent models and the empirical models. Concerning these differences, TOPEX/POSEIDON and ERS-1 crossover statistics unfortunately do not provide conclusive results on which models are in error.  相似文献   

Spectral-temporal structure of variations in the atmospheric total ozone in central Eurasia     

K. N. Visheratin  N. E. Kamenogradskii  F. V. Kashin  V. K. Semenov  V. P. Sinyakov  L. I. Sorokina 《Izvestiya Atmospheric and Oceanic Physics》2006,42(2):184-202

The results of long-term (1980–2003) systematic measurements of the total ozone content at the Issyk Kul station (42.6° N, 77.0° E; 1650 m above sea level) are presented. The statistical characteristics and spectral structure of variations in the total ozone and the main tendencies of its temporal variability are determined. It is found that the total ozone content decreased in 1980–2003 at an average rate of (?1.29±0.08) DU/yr. The results of Fourier and wavelet analyses have shown that only oscillations with periods of 12, 27–29, and 102–105 months are rather stable and can be represented as harmonic oscillations. Oscillations with periods shorter than six months have the character of periodically arising pulsations. Among these, oscillations with periods of 27–29 and 34–37 days can be distinguished. It is noted that the spectral-temporal structure of variations in the total ozone content obtained from ground-based measurements at the Issyk Kul station is in good agreement with the corresponding structure obtained from TOMS satellite measurements.  相似文献   

A detailed survey near Mount Eratosthenes Eastern Mediterranean     

S. J. Tanner  C. A. Williams 《Marine Geophysical Researches》1984,6(2):205-222

A detailed marine survey made to the South West of Mount Eratosthenes is described. The results show the existence of a narrow zone of WNW trending deformed sediment west of the mount surrounded by an evaporite sequence in different stages of deformation.This work was intended to settle the conflict between the results of three previous interpretations of the tectonic structure of this area. We see the area being characterised by five areas with different degrees of tectonic deformation yet showing little causal relationship to overburden of Nile Cone sediments. A fault controlled cliff coincides with the previously described salt wall and strike slip fault yet we see no evidence for its diapiric origin nor positive evidence for strike slip motion along it. We concur with the previously observed area of higher free-air gravity anomalies termed the Eratosthenes tectonic zone, although it is traversed by several boundaries between areas of different deformation. Our results thus show some agreements and some re-interpretations of the observations. This complicated region is not yet fully understood however.  相似文献   

The estimation of gillnet selection curves for Atlantic herring (Clupea harengus L.) using length/girth relations     

Clarke  D. R.; King  P. E. 《ICES Journal of Marine Science》1986,43(1):77-82

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A practical salinity scale     

N. P. Fofonoff  E. L. Lewis 《Journal of Oceanography》1979,35(1):63-64

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Adaptive balance of global development processes     

I. E. Timchenko  E. M. Igumnova  S. M. Solodova 《Physical Oceanography》2007,17(3):173-189

We study the processes of global self-regulation of Earth’s biota (GAIA-theory) by applying the method of adaptive balance of causes proposed by the authors. By using, as an example, the Daisy World model constructed by this method, we reveal the phenomenon of preservation of the mean temperature of Earth’s surface due to the presence of the vegetation cover. We develop an integral model of global natural, social, and economic processes in which the World Ocean is one of the factors regulating the amount of greenhouse gases in the atmosphere. The decrease in the amount of greenhouse gases in the atmosphere caused by the GAIA-effects increases the number of hurricanes and floods on the Earth. As a result, the levels of ecological and social hazards for the mankind become much higher. To eliminate these threats, it is necessary to intensify the processes of self-organization of the society realized via the improvement of education, development of science, and global regulation of the competition for natural resources. We present the results of numerical experiments performed by using the model demonstrating possible scenarios of global development with regard for the processes of self-organization of the society. __________ Translated From Morskoi Gidrofizicheskii Zhurnal, No. 3, Pp. 62–80, May–June, 2007.  相似文献   

The wadic project: A comprehensive field evaluation of directional wave instrumentation   总被引：1，自引：0，他引：1  

J. Allender  T. Audunson  S. F. Barstow  S. Bjerken  H. E. Krogstad  P. Steinbakke  L. Vartdal  L. E. Borgman  C. Graham   《Ocean Engineering》1989,16(5-6)

The results of a comprehensive field trial of nearly all commercially available directional wave measurement systems at the Edda field in the North Sea during winter 1985-86 are presented. The results summarize the accuracy of the principal engineering wave parameters from each system and the dependence on sea state. Limiting factors on system performance and operational problems are also included in the assessment. Overall experience has been good with systems utilizing widely different measurement principles returning consistent results.  相似文献   

The Feni Drift: The importance and meaning of slump deposits on the eastern slope of the Rockall Bank     

J.C. Faugeres  E. Gonthier  F. Grousset  J. Poutiers 《Marine Geology》1981,40(3-4):M49-M57

Sedimentary facies of cores from the eastern side of the Rockall Bank and the Feni Drift (55°–58°N) have been studied. They confirm the existence of slumping which contributes only in small part to drift build-up.

These slumps occurred at different episodes during last Glacial and early Post-Glacial periods. They are sandwiched in between more important turbidites, and are found in places where the drift lies against the slope and so is acted upon by continental-rise sedimentary processes. The sea level rise periods with ice melting seems to favour gravity flow release.  相似文献