东海原甲藻的分子鉴定   总被引：4，自引：2，他引：4  

罗立明  胡鸿钧  李夜光  齐雨藻  吕颂辉  耿亚红  邓光 《海洋学报》2006,28(1):127-131

采用nrDNAITS序列及18S序列两种分子标记,对东海原甲藻和美国国家海洋藻种保藏中心(CCMP)的具齿原甲藻进行分子鉴定,结果表明,两者之间序列非常相似,两者的nrDNAITS序列碱基差异值仅为0.002,nrDNA18S序列的碱基差异值为0.000,根据分子数据,东海原甲藻和美国国家海洋藻种保藏中心的具齿原甲藻应为同一个种.从基因库获取原甲藻的另外3个种nrD-NAITS序列和8个种的18S序列,用计算机软件进行分析和构建分子系统树,结果显示18S序列用于原甲藻的分子鉴定过于保守,而nrDNAITS更适合于该种属界定的分子标准.  相似文献   

三疣梭子蟹消化酶的初步研究   总被引：8，自引：1，他引：8  

胡毅  潘鲁青 《中国海洋大学学报(自然科学版)》2006,36(4):621-626

开发研制配合饲料,以酶学分析方法研究了温度、pH对三疣梭子蟹(Portunus trituberculatus)中肠腺3种主要消化酶(蛋白酶、淀粉酶和纤维素酶)活性的影响及消化酶(蛋白酶、胰蛋白酶、胰凝乳蛋白酶、羧肽酶A、羧肽酶B、淀粉酶、脂肪酶和纤维素酶)在胃、中肠腺、肠道中的活性分布。结果表明:蛋白酶、淀粉酶、纤维素酶的最适温度分别为55℃,55℃,65℃;蛋白酶、淀粉酶、纤维素酶的最适pH分别为7~8,7.5,4.8;蛋白酶、淀粉酶、纤维素酶在短时间内有很高的热稳定性及很广的酸碱稳定性范围。蛋白酶、胰蛋白酶、羧肽酶A、羧肽酶B活性均以中肠腺最高,胃和肠道中没有测出胰凝乳蛋白酶活性;淀粉酶活性在胃、中肠腺、肠道中差别不大;纤维素酶和脂肪酶活性在胃和肠道高于中肠腺。  相似文献   

一种新型潜水器HROV及其关键技术综述   总被引：8，自引：0，他引：8  

沈明学  胡震  刘正元 《海洋工程》2006,24(3):119-123

介绍一种新概念潜水器———混合型潜水器(HROV)。该潜水器能在两种不同的模式下工作,可以像AUV一样进行大范围的探测和搜索,也可以通过直径小于1 mm的微细光纤,执行传统ROV的近距离观察和采样任务。介绍了HROV的系统组成和工作过程,探讨与之相关的关键技术及其主要研究内容,并对HROV技术的应用前景也作了一些分析。  相似文献   

夏季东海西部表层海水中的pCO₂及海-气界面通量   总被引：10，自引：0，他引：10  

谭燕  张龙军  王凡  胡敦欣 《海洋与湖沼》2004,35(3):239-245

根据2001年夏季长江口及东海西部海域表层海水pCO  相似文献   

An ecological study on the fouling organisms in Yantai Harbour     

Li Chuanyan  Huang Zongguo  Wang Jianjun  Zheng Chengxing  Lin Sheng 《海洋学报(英文版)》1991,10(4):629-633

Yantai Harbour (37°33′15″N , 121°23′42″E) is situated at the north coast of Shandong Peninsula and is a large fishing and commercial port of North China. From June 1983 to May 1984, a yearly panel test on fouling organisms was carried out at the non-fixed jetties in the inner and outer harbour. The test method was according to "the Marine Survey  相似文献   

仿鱼尾潜器推进系统的水动力分析   总被引：21，自引：3，他引：21  

苏玉民  黄胜等 《海洋工程》2002,20(2):54-59

以开发适用于小型潜器的仿生操纵与推进系统为研究背景 ,对金枪鱼的月牙形尾鳍进行水动力分析。文中将金枪鱼的尾鳍处理为在做横移和摇摆的耦合运动的同时 ,以某一匀速向前运动的月牙形刚性尾翼。计算中应用了双曲面元和压力库塔条件 ,利用面元法计算分析该三维尾翼的非定常水动力性能。探讨了前进速度、横荡和摇首的幅度、频率及其相位差对推进性能的影响  相似文献   

On the radiation and diffraction of linear water waves by an infinitely long rectangular structure submerged in oblique seas   总被引：1，自引：0，他引：1  

Y.H. Zheng  P.F. Liu  Y.M. Shen  B.J. Wu  S.W. Sheng 《Ocean Engineering》2007,34(3-4):436-450

The radiation and diffraction of linear water waves by an infinitely long rectangular structure submerged in oblique seas of finite depth is investigated. The analytical expressions for the radiated and diffracted potentials are derived as infinite series by use of the method of separation of variables. The unknown coefficients in the series are determined by the eigenfunction expansion matching method. The expressions for wave forces, hydrodynamic coefficients and reflection and transmission coefficients are given and verified by the boundary element method. Using the present analytical solution, the hydrodynamic influences of the angle of incidence, the submergence, the width and the thickness of the structure on the wave forces, hydrodynamic coefficients, and reflection and transmission coefficients are discussed in detail.  相似文献   

浅海内波垂直模式方程的一种解析解     

胡涛  高天赋 《海洋学报》2007,29(4):148-151

给出了一些海区实测浮力频率剖面的数学模型.利用超几何函数对内波垂直模式方程进行了解析求解,得到内波模式方程的解析解和频散方程.利用得到的解析解给出了一种理想条件下内波前三阶简正模式的结构.  相似文献   

Current-influenced depositional provinces, continental margin off Cape Hatteras, identified by petrologic method     

Daniel Jean Stanley  Harrison Sheng  Douglas N. Lambert  Peter A. Rona  David W. McGrail  J.Stacy Jenkyns 《Marine Geology》1981,40(3-4):215-235

Analyses of DSRV “Alvin” core samples on the Cape Hatteras margin indicate major textural and compositional changes at depths of about 1000 and well below 2500 m. The distribution patterns of petrologic parameters correlate well with water mass flow and suspended-sediment plumes measured on this margin by other workers. Our study also shows: (a) vigorous erosion and sediment transport at depths of less than 400 m resulting from the NE-trending Gulf Stream flow; (b) deposition, largely planktonic-rich sediment released from the Gulf Stream, on the upper- to mid-slope, to depths of about 800–1200 m; (c) winnowing, resuspension and deposition induced by periodically intensified slope currents on the mid-slope to uppermost rise, between about 1000 and 2500 m; and (d) prevailing deposition on the upper rise proper (below 2500 m), from transport by the SW-trending Western Boundary Undercurrent. Sediments moved by bottom currents have altered the composition and distribution patterns of material transported downslope by offshelf spillover; this mixing of gravity-emplaced and bottom-current-transported sediment obscures depositional boundaries. Moreover, reworking of the seafloor by benthic organisms alters physical properties and changes erodability of surficial sediments by bottom currents. Measurement of current flow above the seafloor and direct observation of the bottom are insufficient to delineate surficial sediment boundaries. Detailed petrologic analyses are needed to recognize the long-term signature of processes and define depositional provinces.  相似文献   

6个虾种基因组DNA多态性分析   总被引：2，自引：0，他引：2  

许玉德  孙晟 《海洋科学》2001,25(1):9-11

采用RAPD方法检测了罗氏沼虾（Macrobrachium rosenbergii)、绿须虾（Aristeus virilis)、长毛对虾（Penaeus penicillatus)、日本对虾（P.japonicus)、斑节对虾（P.monodon)和周氏新对虾（Metapenaeus joyneri)等6个虾种的基因组DNA的多态性。用20个随机引物扩增得到492个DNA片段，根据这些片段的共享度计算出遗传距离并构建系统树。所得结果从DNA水平上反映出虾类在科属种不同分类阶元亲缘关系的远近，并为虾类现行的分类系统提供了分子生物学依据。  相似文献