大菱鲆幼鱼视网膜结构及不同光谱下视蛋白基因表达特征的研究          下载免费PDF全文

刘峰  吴乐乐  王雨浓  齐婷  徐世宏  李军  李贤 《海洋科学》2023,47(12):30-39

大菱鲆(Scophthalmus maximus)是中国重要的养殖鱼类之一, 其生活史经历不同的栖息地和光环境, 视觉器官结构及视觉功能也具有适应性发育特征和可塑性。本研究以不同发育阶段的大菱鲆幼鱼为对象, 探究了其视网膜结构变化、视蛋白基因的表达特征及其与光谱之间的关系。结果表明, 随着大菱鲆幼鱼的生长发育, 其视网膜的外核层逐渐变厚; 视锥视杆层的厚度变化不明显; 而内核层与神经节细胞层逐渐变薄。视紫红质基因rh1与视蛋白基因总表达量的比例上升, 由2月龄的57.35%上升到9月龄77.19%; 视锥蛋白基因与视蛋白基因总表达量的比例下降; 其中红视蛋白基因lws 由2月龄的4.49%下降至9月龄的0.13%。将7月龄的大菱鲆幼鱼用不同光谱处理75 d后, 其视蛋白基因的表达会随光谱环境的变化而发生改变。与全光谱相比, rh2b1、sws2、sws1在红、黄光下的表达量显著下降, 而rh2b1在蓝、绿光下表达显著上升、sws2在绿光下表达显著上升, 其他则变化不显著(P<0.05)。黄、绿、蓝及全光谱下rh2基因家族与视蛋白基因总表达量的比例最高, 而红光下基因rh1的表达量占比最高。大菱鲆幼鱼在不同阶段以及不同光谱处理下表现出了视蛋白基因表达的可塑性以适应不同水层的光谱环境。本研究为探究大菱鲆对光环境的适应机制及工厂化养殖光照调控技术的建立提供理论参考。  相似文献   

Life habit of vesicomyid clam,Calyptogena soyoae,and hydrogen sulfide concentration in interstitial waters in Sagami Bay,Japan     

Jun Hashimoto  Suguru Ohta  Katsunori Fujikura  Yoshihiro Fujiwara  Shunji Sukizaki 《Journal of Oceanography》1995,51(3):341-350

In and around the beds of vesicomyid clam (Calytogena soyoae) located off Hatsushima Island in Sagami Bay, central Japan, hydrogen sulfide concentration in bottom water and interstitial water was measured every 10 cm from just above seafloor to 40 cm deep usingin situ separative dialysis bags. While hydrogen sulfide over 0.01 mmol/kg was not measured from the seawater just above the dense clam beds, the concentration of hydrogen sulfide increased rapidly below 10 cm deep. The results indicate that the habit of the clam is correlated with high concentration of hydrogen sulfide contained in pore waters of sediments between depths of 10 and 20 cm from the bottom surface. Concentrations of hydrogen sulfide ranging from approximately 0.05 mmol/kg to 0.6 mmol/kg might be suitable requirement for the habitat ofC. soyoae.  相似文献   

海浪的条件特征周期     

徐德伦  刘学海  张军 《海洋学报》2001,23(3):1-7

在海浪波面高度为正态分布的假定下,导出一种以给定波高为条件的条件周期概率密度函数.与风浪槽中测量数据比较,结果表明,在窄谱情况下此概率密度函数与实验室风浪的实际符合良好.根据此密度函数定义了3种条件特征周期,并导出它们与平均周期的关系式.根据这些关系对有关海洋工程上的一些问题作了解释和讨论.  相似文献   

Constraining the origin and evolution of confined turbidite systems: southern Cretan margin,Eastern Mediterranean Sea (34°30–36°N)     

Tiago M. Alves  Vasilios Lykousis  Dimitris Sakellariou  Stamatina Alexandri  Paraskevi Nomikou 《Geo-Marine Letters》2007,27(1):41-61

Bathymetric, 9.5-kHz long-range sidescan sonar (OKEAN), seismic reflection and sediment-core data are used in the analysis of two tectonic troughs south of Crete, Eastern Mediterranean Sea. Here, up to 1.2 s two-way travel time (TWTT) of strata have accumulated since the Middle Miocene in association with extension in the South Aegean region. The study area comprises >100-km- long by >25-km-wide basins filled by sediments subdivided into two seismic units: (1) an upper Unit 1 deposited in sub-basins which follow the present-day configuration of the southern Cretan margin; (2) a basal Unit 2, more than 500 ms (TWTT) thick, accumulated in deeper half-graben/grabens distinct from the present-day depocentres. Both units overlap a locally stratified Unit 3 comprising the pre-Neogene core complex of Crete and Gavdos. In this work, the interpreted seismic units are correlated with the onshore stratigraphy, demonstrating that denudation processes occurring on Crete and Gavdos in response to major tectonic events have been responsible for high sedimentation rates along the proximal southern Cretan margin. Consequently, topographically confined sedimentary units have been deposited south of Crete in the last 12 Ma, including turbidites and other mass-flow deposits fed by evolving transverse and axial channel systems. Surface processes controlling facies distribution include the direct inflow of sediment from alluvial-fan systems and incising mountain rivers onto the Cretan slope, where significant sediment instability processes occur at present. In this setting, seismic profiles reveal eight different types of stratigraphic contacts on basin-margin highs, and basinal areas show evidence of halokinesis and/or fluid escape. The acquired data also show that significant changes to the margin’s configuration occurred in association with the post-Alpine tectonic and eustatic episodes affecting the Eastern Mediterranean.  相似文献   

Action of Cnoidal Waves on Vertical Walls     

Qiu Dahong  Zang Jun  Jia Ying Academician of the Chinese Academy of Sciences  Professor  the State Key Laboratory of Coastal  Offshore Engineering 《中国海洋工程》1996,(2)

Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves  相似文献   

266nm短脉冲激光对泥鳅鱼的胚胎效应     

张闻迪  赵白  邹建华  王秋  葛国昌 《中国海洋大学学报(自然科学版)》1988,(4)

首次使用266nm激光脉冲对泥鳅受精卵进行辐照,引起畸变。辐照分别对全卵、只对胚盘、只对卵黄三种方式进行。对实验结果的分析说明266nm激光对泥鳅受精卵的作用是光化学作用,而不是光致热效应,可能引起DNA分子结构—碱基的变化。核质关系实验证明了细胞质对细脆发育过程有控制作用。  相似文献   

渤海湾海河口区浮游植物动态及其与有机污染的关系     

邹景忠  董丽萍 《海洋科学集刊》1988,(29):131-146

近二十年来，随着海洋环境科学的发展，河口污染和生态问题日益受到重视。关于污水排放对河口内湾浮游植物生态的影响，近年Ketchum, B. H. (1967); McCormick, J.M. 等(1975); Rice, T. R. (1975); Jenkins, S. H. (1978); Thompson, G. B. 等(1981 ); Meyers, V. B. 等(1983)和Joint, I. R.(1984)都做过调查研究和评论。但迄今国内有关这方面的研究报道甚少。 海河口是一个有污染源的生态环境特殊的感潮河口，受自然因素和人类活动的影响很大。由于其地理位置得天独厚，水质肥沃，饵料丰富，历来是许多经济鱼虾类优良的天然产卵、索饵和肥育的场所，在渤海渔业上占有重要位置。但近十多年，由于入海径流量锐减和城市污水排海总量的增加1)，其水质逐渐恶化，环境正由半咸水向海洋化方向演变，对河口渔业构成严重威胁。对此，我所曾于1978-1981年对渤海湾的污染状况及影响进行过多学科的综合调查，在渤海湾污染的浮游植物生态效应、海湾富营养化及其与赤潮的关系等方面，也做过初步研究(邹景忠等，1983; 1984; 1985 )。当时由于受调查范围和项目的限制，对河口有机污染及生态效应的研究还很不够，所获得的一些资料尚不足以作出定量的科学论断。 本文着重探讨海河口区浮游植物的生态变化及其与有机污染的关系，旨在为综合分析和评价海河口区目前的生态质量及演变趋势，为河口环境的综合整治提供科学依据。  相似文献   

Test Study on Current Force on Mooring Ships     

Zhang Furan  Zhao Jun Zhang Xueqin Assistant Researcher  Tianjin Research Institute of Water Transport Engineering  Tianjin Assistant Engineer  Tianjin Research Institute of Water Transport Engineering  Tianjin 《中国海洋工程》1993,(2)

In respect of an offshore berthing pillar, the test study result of current force acting on mooring ships is described in this paper. Empirical and semi-empirical relationships of current force coefficient are given according to the angle between the flow direction and the ship's longitudinal axis, which are coincident with the result of theoretical analysis.  相似文献   

A Computational Model for Velocity Separation in Shallow Sea   总被引：1，自引：0，他引：1  

SONG  Zhiyao 《中国海洋工程》2002,16(3):407-413

Based on the hydrodynamical feature and the theoretical velocity profiles of tidal flow and vvind-induced flow in shal-low sea, a computational model is established for the first time, which can separate observed velocity into tidal velocity and wind-induced velocity by use of the least square method. With the model, not only the surface velocities of tidal flow and vvind-induced flow are obtained, but also the bed roughness height is determined and the wind velocity above the wa-ter surface is estimated. For verification of the model, the observed velocity in the Yellow River Estuary and the laborato-ry test is separated, then it is applied to the Yangtze River Estuary. All the results are satisfactory. The research results show that the model is simple in method, feasible in process and reasonable in result. The model is a valid approach to analysis and computation of field dala, and can be applied to separate the observed velocity in shallow sea; at the same time, reasonable boundary conditions of th  相似文献   

冲绳海槽中段沉积岩芯碳酸盐和烧失量的古环境意义     

吴世迎  刘焱光  王湘芹  白黎明  吴军瑞 《海洋科学进展》2001,19(2):17-24

对冲绳海槽中段 3个沉积岩芯碳酸盐和烧失量的系统分析得知 ,岩芯沉积物的CaCO3含量一般 >1 0 % ,最高含量接近 30 % ,烧失量大都在 1 0 %～ 30 %之间变化 ,反映了该海区处于溶跃面之上钙质生物极为发育的生物地球化学环境。结合δ1 8O曲线所反映的气候环境变化 ,探讨了 1 2 0kaB .P .以来碳酸盐旋回的“大西洋型”演变特点 ,同时对该区沉积地层中的火山热液活动印记作了一定的探讨。  相似文献