Pyrene induces a reduction in midbrain size and abnormal swimming behavior in early-hatched pufferfish larvae     

Yuki Sugahara  Masahumi Kawaguchi  Tatsuya Itoyama  Daisuke Kurokawa  Yasuhiko Tosa  Shin-Ichi Kitamura  Itsuki C. Handoh  Kei Nakayama  Yasunori Murakami 《Marine pollution bulletin》2014

Spills of heavy oil (HO) have an adverse effect on marine life. We have demonstrated previously that exposure to HO by fertilized eggs of the pufferfish (Takifugu rubripes) induces neural disruption and behavioral abnormality in early-hatched larvae. Here, two kinds of polycyclic aromatic hydrocarbons, pyrene and phenanthrene, were selected to examine their toxic effects on larval behavior of another pufferfish species (T. niphobles). Larvae exposed to pyrene or phenanthrene exhibited no abnormalities in morphology. However, those exposed to pyrene but not phenanthrene swam in an uncoordinated manner, although their swimming distance and speed were normal. The optic tectum, a part of the midbrain, of pyrene-exposed larvae did not grow to full size. Thus, these findings are indicated that pyrene might be a contributor to the behavioral and neuro-developmental toxicity, although there is no indication that it is the only compound participating in the toxicity of the heavy oil mixture.  相似文献   

Eruptive history of Sundoro volcano,Central Java,Indonesia since 34 ka     

Oktory Prambada  Yoji Arakawa  Kei Ikehata  Ryuta Furukawa  Akira Takada  Haryo Edi Wibowo  Mitsuhiro Nakagawa  M. Nugraha Kartadinata 《Bulletin of Volcanology》2016,78(11):81

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Elasticity of MgO to 130 GPa: Implications for lower mantle mineralogy     

Motohiko Murakami  Yasuo Ohishi  Naohisa Hirao  Kei Hirose 《Earth and Planetary Science Letters》2009,277(1-2):123-129

The aggregate shear wave velocities of MgO (periclase) have been determined throughout Earth's lower mantle pressure regime approaching 130 GPa using Brillouin spectroscopy in conjunction with synchrotron X-ray diffraction technique in a diamond anvil cell apparatus. We found that the extrapolations of the high-pressure shear wave velocities and shear moduli to ambient pressure are highly consistent with earlier studies. However, the measurements over a wide pressure range revealed that the pressure derivative of the shear modulus (dG/dP = G₀′) of MgO is 1.92(2), which is distinctly lower than that of previous lower-pressure experiments. Compared with the previous results on (Mg,Fe)O ferropericlase, there is no clear correlation between iron content and G₀′. We calculate that the shear wave velocity profile of lower mantle along the adiabatic geotherm applied by the lower G₀′ value of periclase can remarkably well reproduce the global seismological 1-D velocity profile model with uniform composition model. The best-fitting result indicates the possibility of a lower mantle mineralogy with ~ 92 vol.% silicate perovskite phase, implying that the bulk composition of lower mantle is likely not to be pyrolitic but more chondritic. The present acoustic measurements performed over the large pressure range have thus led us to a better understanding of compositional model of the Earth's lower mantle.  相似文献   

Transport properties of the lower mantle     

Kei Hirose  Thorne Lay  David Yuen 《Physics of the Earth and Planetary Interiors》2010,180(3-4):109-110

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Simultaneous volume measurements of post-perovskite and perovskite in MgSiO3 and their thermal equations of state     

Tetsuya Komabayashi  Kei Hirose  Emiko Sugimura  Nagayoshi Sata  Yasuo Ohishi  Leonid S. Dubrovinsky 《Earth and Planetary Science Letters》2008,265(3-4):515-524

Simultaneous volume measurements of MgSiO₃ post-perovskite (PPv) and perovskite (Pv) were performed in a diamond anvil cell (DAC) combined with synchrotron X-rays. An externally-heated DAC was used in addition to a laser-heated DAC for the volume measurement experiment at high temperatures. The volume data were collected in the stability field of post-perovskite from 115 to 130 GPa. The temperature generated in the externally-heated and the laser-heated DACs for the volume measurement were up to 832 and 2330 K, respectively. Using two different but complementary heating techniques, we collected the data at a wide temperature range from 300 to 2330 K. The obtained P-V-T data for PPv and Pv were fitted to a third-ordered Birch-Murnaghan equation of state (EOS). For a precise comparison of the volume between the two phases, the EOSs were constructed based on the same pressure scale of MgO. The simultaneous volume measurements and the volumes calculated from the determined EOSs demonstrate that the volume difference between PPv and Pv of about 1.5% is almost constant with increasing temperature to 4000 K at the transition. At the base of the mantle, this density difference corresponds to a temperature anomaly of 1300 K without the phase transition due to the very small thermal expansivity of minerals, which has a significant effect on mantle dynamics. The thermal expansivity contrast between the top and the bottom of the mantle is a factor of 3.6. From a mantle convection study, this value suggests that huge and hot plumes are formed at the core–mantle boundary.  相似文献   

Does heavy oil pollution induce bacterial diseases in Japanese flounder Paralichthys olivaceus?   总被引：1，自引：1，他引：0  

Jun-Young Song  Kei Nakayama  Yasunori Murakami  Sung-Ju Jung  Myung-Joo Oh  Satoru Matsuoka  Hidemasa Kawakami  Shin-Ichi Kitamura   《Marine pollution bulletin》2008,57(6-12):889

As basic research for the effect of heavy oil on the fish immune system, in this study, the number of leukocyte was counted in Japanese flounder Paralichthys olivaceus, after exposure to heavy oil at a concentration of 30 g/8 L for 3 days. To compare the numbers of bacteria in the skin mucus between oil-exposed and control fish, viable bacteria were enumerated by counting colony forming unit (CFU). Compared with 5.79 ± 1.88 × 10⁷ leukocytes/mL in the controls, the exposed fish demonstrated higher counts, averaging 1.45 ± 0.45 × 10⁸ cells/mL. The bacterial numbers of control fish were 4.27 ± 3.68 × 10⁴ CFU/g, whereas they were 4.58 ± 1.63 × 10⁵ CFU/g in the exposed fish. The results suggest that immune suppression of the fish occurred due to heavy oil stressor, and bacteria could invade in the mucus, resulting in the increasing leukocyte number to prevent infectious disease.  相似文献   

Toxicogenomic analysis of immune system-related genes in Japanese flounder (Paralichthys olivaceus) exposed to heavy oil   总被引：1，自引：1，他引：0  

Kei Nakayama  Shin-Ichi Kitamura  Yasunori Murakami  Jun-Young Song  Sung-Ju Jung  Myung-Joo Oh  Hisato Iwata  Shinsuke Tanabe 《Marine pollution bulletin》2008,57(6-12):445

Heavy oil contamination is one of the most important environmental issues. Toxicities of polycyclic aromatic hydrocarbons (PAHs), including immune toxicities, are well characterized, however, the immune toxic effects of heavy oil, as a complex mixture of PAHs, have not been investigated. In the present study, we selected Japanese flounder (Paralichthys olivaceus) as a model organism, and observed alteration of immune function by the exposure to heavy oil. To analyze the expression profiles of immune system-related genes, we selected 309 cDNAs from our flounder EST library, and spotted them on a glass slide. Using this cDNA array, alteration of gene expression profiles was analyzed in the kidneys of flounders exposed to heavy oil. Six Japanese flounders (mean body weight: 197 g) were acclimated to laboratory conditions at 19–20 °C. Three fish were exposed to heavy oil C (bunker C) at a concentration of 3.8 g/L for 3 days, and the others were kept in seawater without heavy oil and used as the control. After the exposure period, the fish were transferred into control seawater and maintained for 4 days, and then they were dissected and their kidneys were removed. Total RNA was extracted from the kidney samples to use in gene expression analyses. The microarray detected alteration of immune system-related genes in the kidneys of heavy oil-exposed flounders, including down-regulation of immunoglobulin light chain, CD45, major histocompatibility complex class II antigens and macrophage colony-stimulating factor precursor, and up-regulation of interleukin-8 and lysozyme. These results suggest that pathogen resistance may be weakened in heavy oil-exposed fish, causing a subsequent bacterial infection, and then proinflammatory genes may be induced as a defensive response against the infection. Additionally, we found candidate genes for use as biomarkers of heavy oil exposure, such as N-myc downstream regulated gene 1 and heat shock cognate 71 kDa proteins.  相似文献   

Development of a thin diatom layer observed in a stratified embayment in Japan     

Goh Onitsuka  Yutaka Yoshikawa  Tomoyuki Shikata  Kei Yufu  Kazuo Abe  Takahisa Tokunaga  Katsunori Kimoto  Takeshi Matsuno 《Journal of Oceanography》2018,74(4):351-365

The temporal evolution of a thin phytoplankton layer was observed by field measurements using a research vessel and mooring instruments in the Yatsushiro Sea, a semi-enclosed narrow embayment in Japan, in early August 2013. The subsurface chlorophyll maximum developed into a thin layer within 2 days just below the pycnocline at around 10-m depth, where turbulent mixing (the dissipation rate of turbulent kinetic energy) was weak (low). The layer persisted for 1.5 to 2 days and declined after irradiance drastically decreased at the sea surface. At the peak period, the layer thickness, which is defined as the full-width at half-maximum of the peak in chlorophyll a concentration, ranged from 0.6 to 1.4 m, and the maximum concentration reached 42.3 mg m^?3. The horizontal extent of the layer was approximately 10 km along the longitudinal axis of the bay. The phytoplankton population characterized by the layer was dominated by a chain-forming centric diatom, Chaetoceros spp. The formation mechanisms of the thin diatom layer were investigated using the observed data and a vertical one-dimensional model that includes physical and biological processes. The results suggest that the development of the thin layer was caused by in situ growth and aggregation due to nutrient-dependent sinking of the species under weak turbulence. The study highlights that continuous multidisciplinary observations and understanding species-specific physiological responses to environmental variations are necessary to elucidate drastically fluctuating phytoplankton dynamics in a coastal water.  相似文献   

Enrichment of adsorbed methane in authigenic carbonate concretions of the Japan Trench     

Akira Ijiri  Urumu Tsunogai  Toshitaka Gamo  Fumiko Nakagawa  Tatsuhiko Sakamoto  Saneatsu Saito 《Geo-Marine Letters》2009,29(5):301-308

Substantial amounts of adsorbed methane were detected in authigenic carbonate concretions recovered from sedimentary layers from depths between 245 and 1,108 m below seafloor during Ocean Drilling Program Leg 186 to ODP sites 1150 and 1151 on the deep-sea terrace of the Japan Trench. Methane contents were almost two orders of magnitude higher in the concretions (291–4,528 nmol/g wet wt) than in the surrounding bulk sediments (5–93 nmol/g wet wt), whereas methane/ethane ratios and stable carbon isotopic compositions were very similar. Carbonate content of surrounding bulk sediments (0.02–3.2 wet wt%) and methane content of the surrounding bulk sediments correlated positively. Extrapolation of the carbonate contents of bulk sediments suggests that 100 wt% carbonate would correspond to 1,886±732 nmol methane per g bulk sediment, which is similar to the average value observed in the carbonate concretions (1,321±1,067 nmol/g wet wt, n = 13). These data support the hypothesis that, in sediments, adsorbed hydrocarbon gases are strongly associated with authigenic carbonates.  相似文献   

Architecture of growth basins in a tidally influenced,prodelta to delta-front setting: The Triassic succession of Kvalpynten,East Svalbard     

Aleksandra Smyrak-Sikora  Per Terje Osmundsen  Alvar Braathen  Kei Ogata  Ingrid Anell  Mark J. Mulrooney  Valentin Zuchuat 《Basin Research》2020,32(5):949-978

World-class examples of fault-controlled growth basins with associated syn-kinematic sedimentary fill are developed in Upper Triassic prodelta to delta-front deposits exposed at Kvalpynten, SW Edgeøya in East Svalbard. They are interpreted to have interacted with north-westerly progradation of a regional delta system. The syn-kinematic successions consist of 4 to 5 coarsening-upward units spanning from offshore mudstones to subtidal heterolithic bars and compound tidal dunes, which were blanketed by regional, post-kinematic sandstone sheets deposited as laterally continuous, subaqueous tidal dune fields. The rate of growth faulting is reflected in the distribution of accommodation, which governs sedimentary architecture and stacking patterns within the coarsening-upward units. Fully compartmentalized basins (12, 200–800 m wide and c. 150 m high grabens and half grabens) are characterized by syn-kinematic sedimentary infill. These grabens and half-grabens are separated by 60–150 m high horsts composed of pro-delta to distal delta-front mudstones. Grabens host tabular tidal dunes (sandwaves), whereas half-grabens bound by listric faults (mainly south-dipping) consist of wedge-shaped, rotated strata with erosive boundaries proximal to the uplifted fault block crests. Heterolithic tidal bars (sand ridges) occur in narrow half-grabens, showing migration oblique to the faults, up the dipslope. Structureless sandstone wedges and localized subaqueous slumps that formed in response to collapse of the block crests were only documented in half-grabens. Late-kinematic deposition during the final stages of faulting occurred in partly compartmentalized basins, filled with variably thick sets of continuous sandstone belts (compound tidal dunes).  相似文献