Culture-dependent and independent analyses of subsurface microbial communities in oil-bearing strata of the Sagara oil reservoir   总被引：3，自引：0，他引：3  

Takuro  Nunoura  Hanako  Oida  Noriaki  Masui  Fumio  Inagaki  Ken  Takai  Satoshi  Hirano  Kenneth H.  Nealson  Koki  Horikoshi 《Island Arc》2006,15(3):328-337

Abstract   Culture-dependent and independent methods were used to evaluate the microbial communities in cores collected at depths up to 200 m in oil-bearing and oil-free strata near the Sagara oil reservoir near Shizuoka, Japan. Direct microbial counts revealed much higher numbers (2.2 × 10⁴−7.9 × 10⁶/g) of microbes in the oil-bearing strata than in the oil-free zones, where counts were uniform at approximately 1.0 × 10⁴/g. Molecular taxonomic analyses via 16S rRNA gene sequence comparisons showed that the oil-free strata were dominated by members of the γ-Proteobacteria including Pseudomonas , Stenotrophomonas and Sphingomonas , whereas the oil-bearing strata were dominated by a single species closely related to Pseudomonas stutzeri . All archaeal clones were phylogenetically affiliated with the uncultured soil group in Crenarchaeota with the exception of a single phylotype that belonged to the genus Thermococcus . Culture-dependent analysis was carried out by most-probable-number culturing as well as direct plating to determine viable cell counts, using both complex organic substrates or native oil and autotrophic media. Both culture-dependent and independent methods revealed the abundant cultivable member was the aerobic oil-degrading bacterium Pseudomonas stutzeri and neither autotrophs nor anaerobic heterotrophs could be detected in the oil-bearing strata.  相似文献   

Spatio-temporal modelling of the HIV epidemic in Japan based on the national HIV/AIDS surveillance     

Tomoki Nakaya  Katsumi Nakase  Ken Osaka 《Journal of Geographical Systems》2005,7(3-4):313-336

In this paper, we develop a multiregion epidemic model in order to provide the basic information on local HIV epidemic states in Japan. By considering the previous efforts of national epidemic projections in Japan, we statistically calibrate a model by employing an estimation method that allows the inclusion of geographically varying parameters. Using this model, we present a geographical projection of local epidemics until 2015 by examining geographical variations in future epidemic growth and spatial relationships in HIV transmission. We also discuss the implications of planning preventive measures and improvement in the Japanese HIV/AIDS surveillance system based on analytical results.  相似文献   

Melilitite at Fort Portal, Uganda: Another dimension to the carbonate volcanism     

Ken Bailey  Felicity Lloyd  Stuart Kearns  Francesco Stoppa  Nelson Eby  Alan Woolley 《Lithos》2005,85(1-4):15-25

Because the calciocarbonatite lavas at Fort Portal were the first ever described they have received great attention, with the pyroclastic rocks being relatively neglected. Volumetrically the lavas are minute, and the major deposit is a 2 m thick blanket of “flaggy” tuffs, long regarded as carbonatite tuff with crustal debris. Fresh examination shows these tuffs to contain melilitite previously unreported from Fort Portal. The rock is a mix of crust and mantle debris with near-isotropic lapilli, set in a matrix composed predominantly of carbonate. The low birefringence parts of the lapilli are devitrified melilitite glass. Compound lapilli are abundant, containing aggregates of globules, together with xenolithic/crystic fragments. In some, there are concentric zones of more carbonate rich material alternating with melilitite: tangential phlogopite flakes mark the outer zones, in marked contrast to their planar distribution through the enclosing rock matrix. Euhedral titano-magnetite (10–15%) is the most obvious cognate mineral. Devitrified melilitite contains abundant small crystals and microlites of melilite, apatite, magnetite, and carbonates, mostly formed during disequilibrium quench crystallisation. Because of this, and widespread fine grained accidental debris, a precise bulk melt composition is hard to obtain, but the average is close to melilitite with high P₂O₅. Mantle debris is largely disaggregated magnetite–phlogopite clinopyroxenite, which could give a bulk composition close to the melt. Low Mg and high Mg calcite are present in the melilitite lapilli, and in the enclosing carbonate rich matrix. Previously, high Mg calcite was reported only as cement in lapilli tuffs, while the lavas contain only low Mg calcite in the assemblage calcite–periclase (consistent with low pressure carbonate melt crystallisation). Carbonatite–melilitite magma left the mantle carrying restite debris. Melt fragmentation took place in the deep crust, with rapidly quenched droplets enclosing crust debris. Chemical covariations within the flaggy tuffs are uniform and explicable as carbonatite–melilitite plus a thoroughly mixed combination of crust and mantle debris. New links are indicated with the alkaline ultramafic-carbonate volcanism to the south, in Uganda, and parallels with that in Italy.  相似文献   

Mapping and analysing virtual outcrops   总被引：1，自引：0，他引：1  

Immo Trinks  Phillip Clegg  Ken McCaffrey  Richard Jones  Richard Hobbs  Bob Holdsworth  Nick Holliman  Jonny Imber  Steve Waggott  Robert Wilson 《Visual Geosciences》2005,10(1):13-19

Laser scanning is a very efficient way to generate realistic, high-resolution digital models of 3-D geological outcrops. This paper discusses the methodologies involved in the creation and analysis of virtual outcrops, based on laser scanner data. The visualisation of the laser scanner data as a photorealistic 3-D object is described. Geological features picked out on the virtual outcrop (e.g. fractures, faults or bedding planes) can be extrapolated outward, into space, and inward, into the subsurface, using tension surfaces.Electronic Supplementary Material Supplementary material is available in the online version of this article at Reviewed by: J.D. Clemens, D. Yuen  相似文献   

Effects of Flow Regulation in Flow Regime on the Murrumbidgee River,South Eastern Australia: an assessment using a daily estimation hydrological model     

Paul Frazier  Ken Page  Arthur Read 《The Australian geographer》2005,36(3):301-314

In this paper, modelled hydrological data are used to quantify the effects of regulation on the flow regime of the lower Murrumbidgee River in the period 1970–1998. Although other studies report historical changes in flood frequency and duration, this study uses modelled natural daily flow data rather than pre-regulation records or aggregated modelled monthly data. The comparison of modelled natural and regulated daily flows shows the magnitude of changes to mean and seasonal flows, flood peaks and flow duration. At gauges upstream of major irrigation off-takes, mean flows have been increased by approximately 10 per cent, flood peaks have been reduced by 21–46 per cent, and there has been a seasonal redistribution such that flows in summer and autumn have been increased at the expense of those in winter and spring. At gauges downstream of the major irrigation off-takes, mean flows have been reduced by 8–46 per cent, flood peaks have been reduced by 16–61 per cent, and flows have been decreased in all seasons.  相似文献   

Lattice preferred orientation in CaIrO<Subscript>3</Subscript> perovskite and post-perovskite formed by plastic deformation under pressure     

Ken Niwa  Takehiko Yagi  Kenya Ohgushi  Sébastien Merkel  Nobuyoshi Miyajima  Takumi Kikegawa 《Physics and Chemistry of Minerals》2007,34(9):679-686

Lattice preferred orientations (LPO) developed in perovskite and post-perovskite structured CaIrO₃ were studied using the radial X-ray diffraction technique combined with a diamond anvil cell. Starting materials of each phase were deformed from 0.1 MPa to 6 GPa at room temperature. Only weak LPO was formed in the perovskite phase, whereas strong LPO was formed in the post-perovskite phase with an alignment of the (010) plane perpendicular to the compression axis. The present result suggests that the (010) is a dominant slip plane in the post-perovskite phase and it is in good agreement with the crystallographic prediction, dislocation observations via transmission electron microscopy, and a recent result of simple shear deformation experiment at 1 GPa–1,173 K. However, the present result contrasts markedly from the results on MgGeO₃ and (Mg,Fe)SiO₃, which suggested that the (100) or (110) is a dominant slip plane with respect to the post-perovskite structure. Therefore it is difficult to discuss the behavior of the post-perovskite phase in the Earth’s deep interior based on existing data of MgGeO₃, (Mg,Fe)SiO₃ and CaIrO₃. The possible sources of the differences between MgGeO₃, (Mg,Fe)SiO₃ and CaIrO₃ are discussed.  相似文献   

A depth-averaged tidal numerical model using non-orthogonal curvilinear co-ordinates     

Ken George 《Ocean Dynamics》2007,57(4-5):363-374

The two-dimensional shallow-water equations are developed for non-orthogonal curvilinear co-ordinates, in terms of the local deviations of the mesh from east and north. The equations are discretised and applied to a numerical model of tides in the Menai Strait in north Wales. The mesh is designed partially to fit the coastline and deep channels in the domain. After calibration, the model produces results which correspond well with measurements. This research was carried out while on sabbatical leave from University of Plymouth.  相似文献   

Site characterization in densely fractured dolomite: comparison of methods   总被引：2，自引：0，他引：2  

Muldoon M  Bradbury KR 《Ground water》2005,43(6):863-876

One of the challenges in characterizing fractured-rock aquifers is determining whether the equivalent porous medium approximation is valid at the problem scale. Detailed hydrogeologic characterization completed at a small study site in a densely fractured dolomite has yielded an extensive data set that was used to evaluate the utility of the continuum and discrete-fracture approaches to aquifer characterization. There are two near-vertical sets of fractures at the site; near-horizontal bedding-plane partings constitute a third fracture set. Eighteen boreholes, including five coreholes, were drilled to a depth of approximately 10.6 m. Borehole geophysical logs revealed several laterally extensive horizontal fractures and dissolution zones. Flowmeter and short-interval packer testing identified which of these features were hydraulically important. A monitoring system, consisting of short-interval piezometers and multilevel samplers, was designed to monitor four horizontal fractures and two dissolution zones. The resulting network consisted of >70 sampling points and allowed detailed monitoring of head distributions in three dimensions. Comparison of distributions of hydraulic head and hydraulic conductivity determined by these two approaches suggests that even in a densely fractured-carbonate aquifer, a characterization approach using traditional long-interval monitoring wells is inadequate to characterize ground water movement for the purposes of regulatory monitoring or site remediation. In addition, traditional multiwell pumping tests yield an average or bulk hydraulic conductivity that is not adequate for predicting rapid ground water travel times through the fracture network, and the pumping test response does not appear to be an adequate tool for assessing whether the porous medium approximation is valid.  相似文献   

Major perturbation of ocean chemistry and a 'Strangelove Ocean' after the end-Permian mass extinction     

Michael R. Rampino  Ken Caldeira 《地学学报》2005,17(6):554-559

The severe mass extinction of marine and terrestrial organisms at the end of the Permian Period (c. 251 Ma) was accompanied by a rapid (<100 000 years and possibly <10 000 years) negative excursion of c. 3‰ in the δ¹³C of the global surface oceans and atmosphere that persisted for some 500 000 years into the Early Triassic. Simulations with an ocean–atmosphere/carbon-cycle model suggest that the isotope excursion can be explained by collapse of ocean primary productivity, and changes in the delivery and cycling of carbon in the oceans and on land. Model results suggest that severe reduction of marine productivity led to an increase in surface-ocean dissolved inorganic carbon and a rapid, short-term increase in atmospheric pCO₂ (from a Late Permian base of 850 ppm to c. 2500 ppm). Increase in surface ocean alkalinity may have stimulated the widespread microbial and abiotic shallow-water carbonate deposition seen in the earliest Triassic. The model is also consistent with a long-term (>1 Ma) decrease in sedimentary burial of organic carbon in the early Triassic.  相似文献   

Atmospheric Corrections Using MODTRAN for TOA and Surface BRDF Characteristics from High Resolution Spectroradiometric/Angular Measurements from a Helicopter Platform     

Yaping Zhou  Ken C. Rutledge  Thomas P. Charlock  Norman G. Loe  Seiji Kato 《大气科学进展》2001,18(5):984-1004

High-resolution spectral radiance measurements were taken by a spectral radiometer on board a heli copter over the US Oklahoma Southern Great Plain near the Atmospheric Radiation Measurements (ARM) site during August 1998. The radiometer has a spectral range from 350 nm to 2500 nm at 1 nm resolution. The measurements covered several grass and cropland scene types at multiple solar zenith angles. Detailed atmospheric corrections using the Moderate Resolution Transmittance (MODTRAN) radiation model and in-situ sounding and aerosol measurements have been applied to the helicopter measurements in order to re trieve the surface and top of atmosphere (TOA) Bidirectional Reflectance Distribution Function (BRDF) characteristics. The atmospheric corrections are most significant in the visible wavelengths and in the strong water vapor absorption wavelengths in the near infrared region. Adjusting the BRDF to TOA requires a larger correction in the visible channels since Rayleigh scattering contributes significantly to the TOA reflectance. The opposite corrections to the visible and near infrarred wavelengths can alter the radiance dif ference and ratio that many remote sensing techniques are based on, such as the normalized difference vege tation index (NDVI). The data show that surface BRDFs and spectral albedos are highly sensitive to the veg etation type and solar zenith angle while BRDF at TOA depends more on atmospheric conditions and the vi ewing geometry. Comparison with the Clouds and the Earth＇s Radiant Energy System (CERES) derived clear sky Angular Distribution Model (ADM) for crop and grass scene type shows a standard deviation of 0.08 in broadband anisotropic function at 25° solar zenith angle and 0.15 at 50° solar zenith angle, respectively.  相似文献