蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析   总被引：4，自引：0，他引：4  

孟学平高如承  董志国阎斌伦程汉良  李艳杰陈建安 《广东海洋大学学报》2006,26(4):8-13

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。  相似文献   

海岩小说的几种叙述模式及弊端     

姚国军  李霞 《广东海洋大学学报》2006,26(5):87-90

海岩的长篇小说《河流如血》再创出版高记录，但作家仍局限于特有的小说叙述模式，这部作品设能做到相应的创新。概言之，模式第一面是“刑侦＋恋爱”；模式第二面是“财子＋佳人”；模式第三面是“道德＋法制”。文学创作需要不断创新，创新的过程也是对创作模式的不断突破过程。作家应该警惕的是，不要让模式成为创作的桎梏。  相似文献   

Protective effect of topically applied polypeptide from Chlamys farreri against ultraviolet radiation-induced chronic skin damage in guinea pig     

Chi Mingliang  Cao Pengli  Yu Guoying  Zhu Li  Wang Yuejun  Wang Chunbo 《中国海洋湖沼学报》2003,21(4):319-323

Polypeptide fromChlamys farreri (PCF), a topical polypeptide isolated fromChlamys farreri, was used in this experiment aimed to investigate the photoprotective effect of PCF against chronic skin damage induced by ultraviolet A (UVA) and ultraviolet B (UVB) radiation. The chronic ultraviolet-irradiated guinea pig model was established, and visible changes in the skin including wrinkling, sagging and erythema were observed. Malondialdehyde (MDA) and antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in the dorsal skin were determined using biochemical methods. The results showed: (1) PCF (5 % and 20%) could greatly protect the dorsal skin of guinea pig against wrinkling, sagging and erythema induced by UV radiation in a concentration-dependent manner. (2) PCF could reduce MDA formation in the dorsal skin caused by UV irradiation, while increasing the activities of SOD and GSH-px. (3) The differences among the PCF groups and UV model group were significant (P<0.05,P<0.01). These results indicated that topical application, of PCF provided broad solar UV spectrum photoprotection; and that the antioxidant property of PCF might play a role in photoprotection. Project No. 39970638 supported by the NSFC, and the Science and Technology Bureau of Qingdao (No. 2001-28-50).  相似文献   

交通流量分析与实时最优控制     

于凯  薛长虹  覃娟 《成都信息工程学院学报》2006,21(6):882-885

通过将车流量的增大或减小转化为路长权重的变化。将交通流量的动态问题转化为静态问题，用解决最短路问题的Dijkstra方法，给出交通流量实时最优控制的可行性模型及其有效算法。  相似文献   

The efifect of wave breaking on surface wave imaging by Synthetic Aperture Radar     

孙建  管长龙  史剑 《中国海洋湖沼学报》2006,(1)

The effect of ocean wave breaking as a non-Bragg mechanism on backscattering cross-section and modulation transfer functions (MTF) of radar was investigated based on Bragg resonance theory and parametric method. The result showed that the additional effect of wave breaking on backscattering cross-section is not more than 20% except for the small incident angle of VV polarized electromagnetic (e.m.) wave but is significant for HH polarized e.m. wave. Breaking waves lead to increase in the modulus of tilt modulation MTF and the larger the wind speed, the faster the increase. For large incident angle, the modulus of tilt modulation MTF with wave breaking decreases quickly with incident angle for HH polarization and approach to that without wave breaking for VV polarization. The hydrodynamic MTF increases 30%-60% when considering wave breaking and the increase is larger for HH polarization than for VV polarization.  相似文献   

基于蚁群算法的城市可持续发展综合评价   总被引：1，自引：0，他引：1  

徐婷婷  于扬  彭娇婷 《成都信息工程学院学报》2006,21(5):694-697

在可持续发展体系分类的基础上，应用蚁群算法对可持续发展各分类指数公式和综合指数公式进行优化，建立可持续发展评价模型。将该模型应用于乌鲁木齐市可持续发展水平评价，评价结果与现状分析一敛。结果表明该模型简单适用、有良好的通用性。  相似文献   

    

A. S. Belyakov  V. S. Lavrov  A. V. Nikolaev  L. L. Khudzinskii 《Doklady Earth Sciences》2006,407(2):258

  相似文献   

胶东“玲珑-焦家式”金矿资源潜力与找矿   总被引：1，自引：1，他引：0  

李士先  王建收 《山东国土资源》2006,22(12):36-41

“玲珑焦家式”金矿的成岩成矿,从新太古代至中生代存在着继承性的成因关系,成矿具有长期性、多源性特点,但中生代燕山早期岩浆活动是主导成矿因素。区域化探成果显示胶东金矿直接矿源岩———郭家岭花岗岩金的丰度比原始矿源岩———早前寒武纪变质沉积岩系及侵入岩系金的丰度低1/2。郭家岭花岗岩面积在2 km2以上岩体定量估计金亏损总量与胶东金矿总量预测的资源量相当,从一个侧面展现了胶东金矿资源的潜力。望儿山金矿的找矿实践展示,焦家式金矿在1 000 m以下尚有巨大潜力,整个胶东金矿产区在3 000 m以上的资源潜力应在5000 t以上。  相似文献   

Application of Grain Size Analysis to Study Sedimentary Environment of Sand Dune in the Vicinity of Tumen River     

Feng Ma  Li Liu  Lin Cao 《东北亚地学研究》2003,(1)

The dunes in estuary of Tumen River in China lie to the area between the Jiushaping and Fangchuan in the left bank of Tumen River (Fig.1). The dis-tance is about 15～20 km between the dunes and the coast. It ranges from China to D. P. R. Korea and Russia. The range of the height of dunes is about 15～20 m and the width is 100～200 m. By the re-connaissance, we protracted 9 section planes of the dunes and collected 40 sand samples of the dunes, and identified the sedimentary environment …  相似文献   

CCD联合平差方法及其初步实例检验     

YU Yong LI Jinling TANG Zhenghong WANG Guangli ZHAO Ming 《中国科学院上海天文台年刊》2003,(1)

在常规照相天体测量工作中 ,对暗天体的定位是采用逐级定标星过渡的方法实现的。由于星表系统差和局部差的存在 ,最终定位结果可能包含难以把握的误差积累。在CCD小视场观测情况下时常难以找到足够数量的定标星。鉴于此 ,推导了CCD观测联合平差方法的严格矢量表达式 ,以期通过共同星的过渡 ,实现大天区统一平差。原理上此方法可实现相对于河外天体的定位 ,以削弱星表系统差和局部差对定位结果的影响。讨论了CCD联合平差方法实现中的问题 ,包括底片常数模型选择、切点位置改正、共同星的较差改正和法方程解算方法等 ,并分析了具体的处理措施。用实例初步检验了CCD联合平差方法的效果 ,表明通过对多张CCD观测的联合平差 ,可以削弱单张CCD观测参考星数目过少、单张CCD观测参考星分布不均、个别参考星存在位置偏差和局部区域参考星存在系统性位置偏差等不利因素对归算结果的影响 ,进而达到扩大视场和提高归算精度的目的  相似文献