High Precision Geoid Models HKGEOID-2000 for Hong Kong and SZGEOID-2000 for Shenzhen, China     

Zhicai Luo  Jinsheng Ning  Yongqi Chen  Zhanji Yang 《Marine Geodesy》2005,28(2):191-200

High precision geoid models HKGEOID-2000 for Hong Kong and SZGEOID-2000 for Shenzhen, China, have been developed with a hybrid approach of so-called sequential processing, using high precision GPS/leveling data, land and sea gravity anomalies, and digital terrain models. These two local geoid models have the same 1-km resolution. The estimated accuracy (external accuracy) is better than 1.7 cm for HKGEOID-2000 and 1.4 cm for SZGEOID-2000. Some common areas are covered by HKGEOID-2000 and SZGEOID-2000. So these two geoid models, along with high quality GPS/leveling data collected on the overlapping areas, can be used to detect the systematic bias between HKGEOID-2000 and SZGEOID-2000, as well as the difference between Hong Kong Principal Datum and 1956 yellow sea height datum of China, yielding RMS errors of 1.011 m and 1,003 m, respectively. Moreover, HKGEOID-2000, along with GPS ellipsoidal heights, is employed to determine the errors of the “orthometric heights” from purely trigonometric heighting, yielding an RMS error of 0.102 m. The combination of SZGEOID-2000 and GPS ellipsoidal heights has been used to replace the traditional spirit leveling and mapping, called GPS mapping.  相似文献   

利用RAPD和AFLP标记初步构建太平洋牡蛎的遗传连锁图谱   总被引：24，自引：0，他引：24       下载免费PDF全文

李莉  郭希明 《海洋与湖沼》2003,34(5):541-551

利用RAPD和AFLP标记 ,以一回交家系 [(Miyagi×Hiroshima)×Miyagi]为作图群体 ,构建了太平洋牡蛎的遗传连锁图谱。用经过筛选的 3 3个RAPD引物和 1 1个AFLP引物组合 ,对父母本和 80个子代个体进行了遗传分析。共得到母本分离标记 1 93个 ,其中 1 44个符合1∶1孟德尔分离规律。父本分离标记 1 5 6个 ,其中 1 1 1个符合 1∶1孟德尔分离规律。雌性框架图包括 99个遗传标记 ,定位在 1 2个连锁群中 ,覆盖 985 2cM ,标记间平均间隔 1 1 3cM。另外有 3个三联体 ,7个连锁对 ,图谱共覆盖 1 1 6 5 7cM。雄性框架图包括 72个遗传标记 ,分布在 8个连锁群 ,覆盖 81 1cM ,标记间平均间隔 1 2 7cM。另外有 4个三联体 ,3个连锁对 ,图谱共覆盖93 1 8cM。  相似文献   

Diagnosis of iridovirus in large yellow croaker by PCR   总被引：1，自引：0，他引：1  

Chen Xinhu  Wang Xiaowen  Wu Wenzhong 《海洋学报(英文版)》2003,22(4):635-642

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.  相似文献   

In-Situ Observation of Fluid Mud in the North Passage of Yangtze Estuary, China   总被引：1，自引：0，他引：1  

李九发  万新宁何青应铭  时连强S.M.Hutchinson 《中国海洋工程》2004,18(1):149-156

Observations of fluid mud were made in the lower North Passage of the Yangtze Estuary in February 2000, on 10 -11 August 2000, on 30 - 31 August 2000 (after two strong typhoons), on 21 - 24 August 2000 (neap tide) and on 3 -6 September 2000 (mean tide) respectively. In situ data show that the fluid mud in this area consists of fine cohesive sediment (median size 7.23 μm). The formation and movement of fluid mud varied during the neap-spring and flood-ebb tidal cycle. Observations suggest that fluid mud phenomena in this area may be categorised in a three-fold manner as slack water, storm and saltwedge features. The thickness of the fluid mud layer of slack water during the neap tide ranged from 0.2 to 0.96 m, whereas during the mean tide, the thickness ranged from 0.17 to 0.73 m, and the thickness of the fluid mud layer was larger during slack water than at the flood peak. Shoals cover an area of 800 km^2 with a water depth smaller than 5 m. Erosion of these extensive intertidal mudflats due to storm action provides an abundant sediment source. This is particularly significant in this estuary when the tidal level is lower than 5 m. The lower North Passage is a typical zone of saltwater wedging, so the saltwedge fluid mud has the most extensive spatial range in the estuary.  相似文献   

泉州湾水域浮游植物群落的昼夜变化   总被引：2，自引：1，他引：2  

唐森铭  陈兴群 《海洋学报》2006,28(4):129-137

对福建省泉州湾口、大坠岛以北水域的浮游植物群落作了准同步昼夜连续观测,观测时间分别在2001年11月至2002年8月内的秋(11月)、冬(2月)、春(5月)、夏(8月)季大潮期.结果表明,该水域的叶绿素a生物量(以下简称生物量)受湾内高生物量的影响在潮汐作用下出现波动,高潮期出现低生物量,低潮期出现高生物量.靠近内湾的站位生物量大于湾外的站位;底层水体中的生物量普遍大于表层,底栖硅藻成为生物量变化的重要部分.调查海区生物量呈现昼夜节律变化,生物量白天高于夜间.进一步的分析表明,在潮汐和昼夜变化综合作用下,浮游植物的群落结构发生相应变化,白天的多样性指数低于夜间,浮游植物昼夜群落的不相似度很高.群落中一些种类如中肋骨条藻白天大量增殖,出现较高的优势度,昼夜平均密度比值达到9,变化节律明显.研究认为,尽管海区潮汐和流向左右着生物量变化,浮游植物的生态学过程如细胞增殖、再悬浮、扩散等过程在昼夜生物量变化中仍产生较大作用.  相似文献   

Changjiang River sediment delivering into the sea in response to water storage of Sanxia Reservoir in 2003   总被引：3，自引：0，他引：3  

CHU Zhongxin  ZHAI Shikui  CHEN Xiufa 《海洋学报(英文版)》2006,25(2):71-79

1 IntroductionHuman activitieshavebeen stronglyaffecting theworld river sedimentsupply to the oceans since the19thor20thcentury(StanleyandW arne,1993;Fanos,1995;Sm ithandW inkley,1996;Stanley,1996;Yang,Shi,etal.,2004;Yang,Zhao,etal.,2002).Thesedi-m enttra…  相似文献   

Sediment transport pattern at the Barra Nova inlet, south Portugal: a conceptual model     

Yann Balouin  Hélène Howa 《Geo-Marine Letters》2002,21(4):226-235

The Barra Nova inlet, in south Portugal, is known to migrate progressively southeastwards under wave action. The morphodynamics of this system during a representative year suggests that this long-term evolution is dependent on a seasonal behavior of the tidal inlet which can be described through a three-stage model of post-storm, transition and extended fair-weather conditions. Processes involved in this evolution indicate that the historical migration of the Barra Nova is not dependent on the longshore drift forcing constricting the channel on the updrift coast, but rather on the adjustment of the system to a major erosion of the downdrift coast during short storm events.  相似文献   

耳鲍(Haliotis asinina)核糖体小亚基(18S rRNA)编码基因的克隆与序列分析   总被引：1，自引：0，他引：1       下载免费PDF全文

黄勃  方再光  刘均玲  周智  王小兵 《海洋与湖沼》2007,38(3):212-216

采用分子生物学的方法,对南海不同海区的两个地理群体耳鲍(Haliotis asinina)18S rRNA基因全长进行了克隆和序列分析,并将耳鲍18S rRNA基因的序列与NCBI数据库中已收录鲍的18SrRNA基因进行了比较。结果发现,南海耳鲍核糖体18S rRNA基因与耳鲍H.asinina isolate H11核糖体18S rRNA基因序列的相同率高达98%;同一地理群体内耳鲍核糖体18S rRNA基因序列完全一致;不同地理群体间耳鲍核糖体18S rRNA基因在碱基组成上的相似率为99%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中耳鲍的18S rRNA基因与泰国耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。  相似文献   

棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响     

刘文斌  王恬 《海洋与湖沼》2006,37(6):568-574

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。  相似文献   

刀额新对虾染色体核型及细胞核DNA含量   总被引：9，自引：0，他引：9  

张晓军  周岭华  相建海 《海洋与湖沼》2002,33(3):225-231

以刀额新对虾胚胎、无节幼体、卵巢、精巢等为材料 ,空气干燥法制备染色体 ,并初步进行核型分析。结果表明 ,刀额新对虾的染色体数目为 2n=78,n =39,核型为 2n=78=40M 1 0SM 1 4ST 1 4T。以刀额新对虾血淋巴、卵巢、肌肉、鳃为材料 ,以鸡血细胞为DNA标准 (2 50pg/ 2c) ,使用PartecCCAⅡ型流式细胞仪测定了刀额新对虾细胞的基因组DNA含量 ,其值为鸡血细胞的 1 75倍 ,绝对含量为 4 375pg/ 2c。  相似文献