Influence of Ultrasonic Treatment on the Allergenic Properties of Shrimp （Penaeus vannamei ） Allergen     

LI Zhenxing LIN Hong CAO Limin 《中国海洋大学学报(英文版)》2006,5(2):115-118

The present study was undertaken to determine whether high intensity ultrasound could reduce the allergic properties of shrimp allergens. Reducing the allergenic properties of these allergens will be beneficial to allergic individuals. Samples of shrimp protein extract and shrimp muscle were treated by high-intensity ultrasound with water bathing at 0 ℃ or 50 ℃ for different time periods. The treated and untreated samples were then analyzed by SDS-PAGE, Western blots and competitive inhibition ELISA （Ci-ELISA） to determine the shrimp allergenicity. The results show that high-intensity ultrasound has no effect on allergenicity when the extracts were treated at 0℃. However, a significant decrease was observed in the level of the major shrimp allergen, Pen a 1, when the samples were treated at 50 ℃. In the determination of allergenicity with CiELISA, a reduction in IgE binding was also observed.  相似文献   

斑节对虾白斑综合症杆状病毒感染的实验模式     

邱德全  林继辉  刘丽  张凌飞 《广东海洋大学学报》2001,21(1):10-13

在建立斑节对虾实验室养殖模式的基础上，对野外采集的有白斑综合症病毒（WSSV）病典型症状的斑节对虾初步纯化其病毒，并进行WSSV的PCR检测，得到WSSV的感染用样品。对健康斑节对虾分别进行浸浴感染，投喂感染和注射感染。对感染死亡个体进行WSSA的PCR检测和细菌检测，证实WSSV感染性和致死性。浸浴感染、投喂感染和注射感染的感染量分别为4mL/L、0.2g/10g虾体、1/2稀释液0.05mL/10g虾体，死亡开始时间分别为16d、42h、28h，三种感染模式最终死亡率100%，从开始死亡到全部死亡延续时间分别为15d、82h、44h。  相似文献   

On the Line Formation in Stellar Magnetized Atmospheres     

Zhong-Quan Qu  Xiao-Yu Zhang  Zhi Xu 《中国天文和天体物理学报》2001,1(2):161-175

1 INTRODUCTIONIn the previous paper (on et al. 1999, hereafter Paper I), we investigated the wavelength-dependence of four colltribution functions (CFs) derived from dmerent formal solutions andreferring to different emergellt quantities in the unpolarized case. Because one cannot generallyassign a single formation region to the whole line band in a real stellar atmosphere, e.g., the solaratmosphere, instead, the line formation region can be defined as the layers deviating farthestfrom t…  相似文献   

量子化学方法研究氯代苯酚类化合物对水生物毒性的构效关系     

许旋  罗一帆  李华祥 《广东海洋大学学报》1992,(2)

对20个氯代苯酚类化合物进行了CNDO/2量子化学计算,讨论了化合物电子结构与其对水生物翻车鱼(Bluegill)、花鳉鱼(Guppy)、和虾(Shrimp)半致死量之间的关系,分别获得了表示其构效关系的三个线性方程,它们的显著性均远高于α=0.01水平。结果表明:氯代苯酚化合物苯环上碳原子的兀电荷之和(∑Qπ_R)越大苯环上碳原子的净电荷之和(∑Q_R)越大,或LUMO轨道能(E_(LUMO))越低,均使化合物对Bluegill和Guppy水生物的毒性增大;而化合物苯环上羟基邻位的碳原子的兀电荷布居(P_3)越大,同时HOMO轨道能E_(HOMO)越高,则化合物对Shrimp毒性也越大。据此,可预测氯代苯酚类化合物对上述三种水生物的毒性。  相似文献   

On the calculation of perturbations in the radial position of satellites     

Lin Liu 《Chinese Astronomy and Astrophysics》1997,21(4):488-500

With the aim of analyzing the errors in the radial position of satellites, we give in this paper the expressions for the radial perturbation which include the complete zeroth- and first-order terms in the orbital eccentricity. A simpler and faster method of calculating the perturbation is given.  相似文献   

山西省石炭系与二叠系界线     

林联情 《河南国土资源》1995,(1)

１８８２年李希霍芬在太原西山建立石炭系的太原系含煤建造以来，石炭系与二叠系的分界意见，归纳起来有三：①以Ｐｓｅｕｄｏｓｃｈｗａｇｅｒｉｎａ（ｓ．１）带之底为界，下面为石炭系，上面为二叠系；②以晋东南的灰白色“佳祥砂岩”与太原西山黄绿色“骆驼脖子砂岩”对比，砂岩底面为石炭系与二叠系的分界线，下面是石炭系山西组，上面是二叠系石盒子组，山西组的地层时代定为晚石炭世；③以晋东南灰白色第三砂岩（陵川砂岩）与太原西山“北岔沟砂岩”对比，砂岩底面为石炭系与二叠系的分界线，下面是石炭系太原组，上面是二叠系山西组，山西组的地层时代为早二叠世。笔者认为石炭系与二叠系的划分，以第③方案较适宜。  相似文献   

Numerical calculations in the orbital determination of an artificial satellite for a long arc     

Lin Liu  Xinhao Liao 《Celestial Mechanics and Dynamical Astronomy》1994,59(3):221-235

Numerical methods are usually used for the computation of ephemerides with perturbations for the precise orbital determination of an artificial satellite. But their numerical stability will be encountered in a long arc. In this case the use the improved Encke special perturbation methods has been suggested. The results of this paper show that Encke's method does indeed have a certain effectiveness, but cannot yet completely resolve the numerical stability, and the more efficient method is to use the energy integral or its variational relation to control the growth of the along-track error in general numerical calculations so that the aim of stabilization can be achieved.  相似文献   

广东省拖网网囊调查     

杨吝  张旭丰  孙典荣  谭永光 《广东海洋大学学报》2001,21(4):22-26

为实施中华人民共和国国家标准《南海区拖网网囊最小网目尺寸》,对广东省范围内拖网网囊现状进行摸底调查,结果表明,广东省沿海拖网渔船现用网囊网目尺寸(内径)在16.7～35.2 mm范围内,总体平均为27 mm,均未能达到1989年国家技术监督局发布的中华人民共和国国家标准--《南海区拖网网囊最小网目尺寸》--内径39 mm.  相似文献   

Effective collision strengths with inclusion of resonance for hyperfine-structure transitions in H- and Li-like ions     

Hong Lin Zhang  Douglas H. Sampson 《Monthly notices of the Royal Astronomical Society》2001,322(3):433-437

Calculations are made of the resonance contribution to electron-impact excitation of H-like ¹³C and Li-like ²³Na, ²⁵Mg, ²⁷Al and ²⁹Si to the upper hyperfine levels that produce millimetre (mm) lines of likely astrophysical interest. The resonance contribution is found to be very important for these Li-like ions, considerably more important than for Li-like ⁵⁷Fe considered previously. However, resonances are found to be rather unimportant for H-like ¹³C. The effect of radiative decay on the resonance contribution is found to be insignificant in all of the present calculations.  相似文献   

Determination of physical parameters of NGC 2023 using the hyperfine NH₃ spectrum     

Xing Jun  Xu Lan-Ping 《Chinese Astronomy and Astrophysics》1985,9(4):283-290

An improved approach is given for deriving the physical parameters of a molecular cloud by its NH₃ rotation-inversion hyperfine spectra. The optical depth τ₀(1, 1) of NH₃ (1,1) is obtained by considering the blending effect of the magnetic hyperfine spectral lines, the (1-1) excitation temperature T_ex (1, 1) is calculated by a two level model, the effect of different collision rate and the thermalization of the (1, 1) inversion lines are discussed, the rotation temperature T_R ( 2, 1) between the NH₃ levels (2, 2) and (1, 1) and the column density N( 1, 1) of the NH₃ (1, 1) inversion level are derived, the results of the total column density from different assumptions for the abundance ratio of ortho-NH₃ and para-NH₃ are also discussed.

This approach is used for the molecular cloud NGC 2023 by using the relevant observed data and its optical depth and other physical parameters are obtained.  相似文献