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81.
cDNA clones for glutathione S-transferases A (GST-A) and A1 (GST-A1) from plaice (Pleuronectes platessa) were expressed as N-terminally 6XHis tagged proteins in Escherichia coli and purified to homogeneity from Ni-NTA silica. GST-A was an efficient catalyst for conjugation of unsaturated alkenals derived from peroxidation of polyunsaturated fatty acids with the highest activity observed with trans-non-2-enal (8 micromol min(-1) mg(-1)). GST-A1 was a very efficient Se-independent glutathione peroxidase with an activity towards cumene hydroperoxide of 25 micromol min(-1) mg(-1). Although the enzymes exhibited moderately high activities towards the model substrate 1-chloro-2,4-dinitrobenzene (CDNB) they exhibited little or no activity towards other common prototypical xenobiotic substrates. Together with data for ontogeny, tissue distribution and inducibility of these enzymes, we contend that a primary function of these enzymes is protection from the harmful effects of lipid peroxidation products generated naturally or exacerbated by xenobiotic exposure. 相似文献
82.
We present a linear Boltzmann equation to model wave scattering in the Marginal Ice Zone (the region of ocean which consists of broken ice floes). The equation is derived by two methods, the first based on Meylan et al. [Meylan, M.H., Squire, V.A., Fox, C., 1997. Towards realism in modeling ocean wave behavior in marginal ice zones. J. Geophys. Res. 102 (C10), 22981–22991] and second based on Masson and LeBlond [Masson, D., LeBlond, P., 1989. Spectral evolution of wind-generated surface gravity waves in a dispersed ice field. J. Fluid Mech. 202, 111–136]. This linear Boltzmann equation, we believe, is more suitable than the equation presented in Masson and LeBlond [Masson, D., LeBlond, P., 1989. Spectral evolution of wind-generated surface gravity waves in a dispersed ice field. J. Fluid Mech. 202, 111–136] because of its simpler form, because it is a differential rather than difference equation and because it does not depend on any assumptions about the ice floe geometry. However, the linear Boltzmann equation presented here is equivalent to the equation in Masson and LeBlond [Masson, D., LeBlond, P., 1989. Spectral evolution of wind-generated surface gravity waves in a dispersed ice field. J. Fluid Mech. 202, 111–136] since it is derived from their equation. Furthermore, the linear Boltzmann equation is also derived independently using the argument in Meylan et al. [Meylan, M.H., Squire, V.A., Fox, C., 1997. Towards realism in modeling ocean wave behavior in marginal ice zones. J. Geophys. Res. 102 (C10), 22981–22991]. We also present details of how the scattering kernel in the linear Boltzmann equation is found from the scattering by an individual ice floe and show how the linear Boltzmann equation can be solved straightforwardly in certain cases. 相似文献
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87.
Michael E. McCormick 《Ocean Engineering》1976,3(3):133-144
The analysis of a pneumatic-type wave-energy conversion buoy is developed assuming independence of the buoy heaving motion and the motion of the water column within the center pipe. Results of the analysis are then compared with experimental data in a study of the relative air velocity within the turbine passage. The results compare very well. The effect of the variation of the center pipe length is found to be significant for periods about the surge chamber resonance but is negligible in the neighborhood of the heaving resonance period. Further, the theory is applied to a prototype buoy study of the U.S. Coast Guard, and a dimensionless design curve is developed from the results of the prototype analysis. 相似文献
88.
Michael MccGwire 《Marine Policy》1980,4(4):317-322
The purpose of this article is to draw attention to a change in the trend of Soviet naval developments. This change stems from a combination of: (1) a sharp increase in the allocation of resources to naval shipbuilding; (2) a marked rise in the navy's political influence; and (3) a new approach to the role of seapower in Soviet policy. Professor MccGwire, Senior Fellow at the Brookings Institution, USA, believes that both the nature and the significance of the change are being obscured by the fact that for the past decade naval leaders in the West have been talking of ‘a Soviet naval build-up’, and continue to use the same language to describe the very different present situation. There is no general awareness, even within naval circles, that we are now facing a significant change in established trends. This article draws on a longer and more general article.1 相似文献
89.
Two processes are generally explained as causes of temporal changes in the stoichiometric silicon/nitrogen (Si/N) ratios of
sinking particles and of nutrient consumption in the surface water during the spring diatom bloom: (1) physiological changes
of diatom under the stress of photosynthesis of diatom and (2) differences of regeneration between silicon and nitrogen. We
investigated which process plays an important role in these changes using a one-dimensional ecosystem model that explicitly
represents diatom and the other non-silicious phytoplankton. The model was applied to station A7 (41°30′ N, 145°30′ E) in
the western North Pacific, where diatom regularly blooms in spring. Model simulations show that the Si/N ratios of the flux
exported by the sinking particles at 100 m depth and of nutrient consumptions in the upper 100 m surface water have their
maxima at the end of the spring diatom bloom, the values and timings of which are significantly different from each other.
Analyses of the model results show that the differences of regeneration between silicon and nitrogen mainly cause the temporal
changes of the Si/N ratios. On the other hand, the physiological changes of diatoms under stress can hardly cause these temporal
changes, because the effect of the change in the diatom's uptake ratio of silicon to nitrogen is cancelled by that in its
sinking rate. 相似文献
90.
Several large deployments of neutrally buoyant floats took place within the Antarctic Intermediate (AAIW), North Atlantic Deep Water (NADW), and the Antarctic Bottom Water (AABW) of the South Atlantic in the 1990s and a number of hydrographic sections were occupied as well. Here we use the spatially and temporally averaged velocities measured by these floats, combined with the hydrographic section data and various estimates of regional current transports from moored current meter arrays, to determine the circulation of the three major subthermocline water masses in a zonal strip across the South Atlantic between the latitudes of 19°S and 30°S. We concentrate on this region because the historical literature suggests that it is where the Deep Western Boundary Current containing NADW bifurcates. In support of this notion, we find that a net of about 5 Sv. of the 15–20 Sv that crosses 19°S does continue zonally eastward at least as far as the Mid-Atlantic Ridge. Once across the ridge it takes a circuit to the north along the ridge flanks before returning to the south in the eastern half of the Angola Basin. The data suggest that the NADW then continues on into the Indian Ocean. This scheme is discussed in the context of distributions of dissolved oxygen, silicate and salinity. In spite of the many float-years of data that were collected in the region a surprising result is that their impact on the computed solutions is quite modest. Although the focus is on the NADW we also discuss the circulation for the AAIW and AABW layers. 相似文献