Depuration dynamics of oysters (Crassostrea gigas) artificially contaminated by Salmonella enterica serovar Typhimurium     

de Abreu Corrêa A  Albarnaz JD  Moresco V  Poli CR  Teixeira AL  Oliveira Simões CM  Monte Barardi CR 《Marine environmental research》2007,63(5):479-489

The State of Santa Catarina produces the greatest quantity of edible mollusks in Brazil. To guarantee sanitary qualify, mollusk cultures should be monitored for contamination by pathogenic microorganisms. A self-purification or "depuration" system that eliminates Salmonella enterica serovar Typhimurium contamination from oysters has been developed and evaluated. The depuration process occurred within a closed system, in which 1000 L of water was recirculated for 24 h. The water was sterilized with ultraviolet (UV) light, chlorine, or both together. Oysters (Crassostrea gigas) artificially contaminated with S. typhimurium were harvested every 6 h. Samples of oyster tissue were excised and both the presence and numbers of bacteria were determined. Combined UV light and chlorine treatments resulted in total elimination of bacteria within 12 h. Polymerase chain reaction detected bacteria in water exposed to the three treatments. This pioneering study is the first of its kind in Brazil and represents a major contribution to commercial mollusk culture in this country.  相似文献   

Carbonaceous materials in size-segregated atmospheric aerosols from urban and coastal-rural areas at the Western European Coast   总被引：1，自引：1，他引：0  

Regina M.B.O. Duarte  Cludia L. Mieiro  Ana Penetra  Casimiro A. Pio  Armando C. Duarte 《Atmospheric Research》2008,90(2-4):253-ICNAA07

A high-volume cascade impactor, equipped with a PM10 inlet, was used to collect size-segregated aerosol samples during the summer of 2004 at two Portuguese locations: a coastal-rural area (Moitinhos) and an urban area (Oporto). Concentrations of airborne particulate matter (PM), total carbon (TC), organic carbon (OC), elemental carbon (EC), and water-soluble organic carbon (WSOC) were determined for the following particle size ranges: < 0.49, 0.49–0.95, 0.95–3.0, and 3.0–10 µm. The total PM mass concentrations at the urban and coastal-rural sites ranged from 22.8 to 79.6 μg m^− 3 and 19.9 to 28.2 μg m^− 3, respectively, and more than 56% of the total aerosol mass was found in the fractions below 3.0 μm. At both locations the highest concentrations of OC and EC were found in the submicrometer size range. The regional variability for the OC and EC concentrations, with the highest concentrations being found in the urban area, was related to the contribution of local primary sources (mostly traffic emissions). It was also verified an enrichment of the small size particles in WSOC, representing on average 37.3(± 12.4)% and 59.7(± 18.0)% of OC in the very fine aerosol at the coastal-rural and urban areas, respectively. The amount of secondary OC calculated by the minimum OC/EC ratio method indicates that secondary organic aerosol formation was important throughout the study at both sites. The obtained results suggest that long-range transport and favourable summer conditions for photochemical oxidation are key factors determining secondary OC formation in the coastal-rural and urban areas. The ultraviolet absorption properties of the chromophoric constituents of the WSOC fractions were also different among the different particle size ranges and also between the two sampling locations, thus suggesting the strong impact of the diverse emission sources into the composition of the size-segregated organic aerosol.  相似文献   

LINET—An international lightning detection network in Europe   总被引：1，自引：0，他引：1  

Hans D. Betz  Kersten Schmidt  Pierre Laroche  Patrice Blanchet  Wolf P. Oettinger  Eric Defer  Z. Dziewit  J. Konarski 《Atmospheric Research》2009,91(2-4):564-573

During the past years a VLF/LF lightning detection network (LINET) was developed at the University of Munich, which provides continuous data for both research and operational purposes. In particular, the network introduces five new features: a) total lightning capability: both cloud-to-ground strokes (CG) and cloud lightning (IC) are measured; b) low-amplitude reporting: weak lightning events from discharge channel with currents well below 5 kA are detected within the central part of the network, whereby IC events dominate; c) new 3D-discrimination: a time-of-arrival method is utilized to separate CG from IC with good reliability, provided that the sensor baseline does not exceed ~ 250 km; d) IC emission height: for each cloud event a height is determined which is thought to reflect the central region of the involved channel; and e) optimised location accuracy: due to precision and combined action of all influential network components, complemented by site-error corrections, the position accuracy of strokes reaches an average value as small as ~ 150 m, whereby false locations (‘outliers’) rarely occur. During international co-operations LINET has been deployed in four continents: Europe (initially Germany), South America (area of Bauru, Brazil), Australia (around Darwin), and Central Africa (Benin). Since the features quoted above could be verified in the tests, a 65-sensor network was established in Europe and started on May 1, 2006, in co-operation with the service company nowcast. LINET covers a wide area approximately from longitude − 10° to 25° to latitude 35° to 66°; it is available for scientific projects and officially utilized by the German Weather Service for operational purposes. Meanwhile, the network was extended by deployment of additional sites so that it comprises about 90 sensors in 17 countries.  相似文献   

Geochemical modeling of arsenic sulfide oxidation kinetics in a mining environment     

Maggy F. Lengke  Regina N. Tempel 《Geochimica et cosmochimica acta》2005,69(2):341-356

Arsenic sulfide (AsS (am), As₂S₃ (am), orpiment, and realgar) oxidation rates increase with increasing pH values. The rates of arsenic sulfide oxidation at higher pH values relative to those at pH∼2 are in the range of 26-4478, 3-17, 8-182, and 4-10 times for As₂S₃ (am), orpiment, AsS (am), and realgar, respectively.Numerical simulations of orpiment and realgar oxidation kinetics were conducted using the geochemical reaction path code EQ3/6 to evaluate the effects of variable DO concentrations and mineral reactivity factors on water chemistry evolution during orpiment and realgar oxidation. The results show that total As concentrations increase by ∼1.14 to 13 times and that pH values decrease by ∼0.6 to 4.2 U over a range of mineral reactivity factors from 1% to 50% after 2000 days (5.5 yr). The As release from orpiment and realgar oxidation exceeds the current U.S. National Drinking Water Standard (0.05 ppm) approximately in 200-300 days at the lowest initial dissolved oxygen concentration (3 ppm) and a reactivity factor of 1%. The results of simulations of orpiment oxidation in the presence of albite and calcite show that calcite can act as an effective buffer to the acid water produced from orpiment oxidation within relatively short periods (days/months), but the release of As continues to increase.Pyrite oxidation rates are faster than orpiment and realgar from pH 2.3 to 8; however, pyrite oxidation rates are slower than As₂S₃ (am) and AsS (am) at pH 8. The activation energies of arsenic sulfide oxidation range from 16 to 124 kJ/mol at pH∼8 and temperature 25 to 40°C, and pyrite activation energies are ∼52 to 88 kJ/mol, depending on pH and temperature range. The magnitude of activation energies for both pyrite and arsenic sulfide solids indicates that the oxidation of these minerals is dominated by surface reactions, except for As₂S₃ (am). Low activation energies of As₂S₃ (am) indicate that diffusion may be rate controlling.Limestone is commonly mixed with sulfide minerals in a mining environment to prevent acid water formation. However, the oxidation rates of arsenic sulfides increase as solution pH rises and result in a greater release of As. Furthermore, the lifetimes of carbonate minerals (i.e., calcite, aragonite, and dolomite) are much shorter than those of arsenic sulfide and silicate minerals. Thus, within a geologic frame time, carbonate minerals may not be present to act as a pH buffer for acid mine waters. Additionally, the presence of silicate minerals such as pyroxenes (wollastonite, jadeite, and spodumene) and Ca-feldspars (labradorite, anorthite, and nepheline) may not be important for buffering acid solutions because these minerals dissolve faster than and have shorter lifetimes than sulfide minerals. However, other silicate minerals such as Na and K-feldspars (albite, sanidine, and microcline), quartz, pyroxenes (augite, enstatite, diopsite, and MnSiO₃) that have much longer lifetimes than arsenic sulfide minerals may be present in a system. The results of our modeling of arsenic sulfide mineral oxidation show that these minerals potentially can release significant concentrations of dissolved As to natural waters, and the factors and mechanisms involved in arsenic sulfide oxidation warrant further study.  相似文献   

Book reviews     

Regina Tempel  Jaak Daemen  Donald E. Myers 《Mathematical Geosciences》1996,28(6):819-826

  相似文献   

Assessing streams in Germany with benthic invertebrates: Development of a multimetric invertebrate based assessment system   总被引：3，自引：0，他引：3  

Jürgen Bhmer  Claudia Rawer-Jost  Armin Zenker  Carolin Meier  Christian K. Feld  Regina Biss  Daniel Hering 《Limnologica》2004,34(4):416-432

The main aim of the study was to develop a multimetric assessment system for macroinvertebrate communities of small and mid-sized streams in Germany in accordance with the Water Framework Directive of the European Union. The system should be applicable by water authorities, capable of distinguishing between different impacts, and specifically adapted to all types of small and mid-sized rivers in Germany. An assessment system has been developed consisting of two modules based on the established indices for saprobic degradation and acidity, and a third module covering general degradation. For the third module a new multimetric index (MMI) was developed and tested using preclassifications for stream hydromorphological degradation, land use and general impairment as impact measures. More information on sampling method, season and the type of dominating human impairment allowed to further analyse the index' capabilities and sensitivity. It performed well in all seasons and detected all types of impairment except acidity. The usefulness of the impact measures used in this study is discussed with regard to the development of a multimetric index. The comparison of the new index with other indices showed that it is especially sensitive in detecting macroinvertebrate community responses to hydromorphological degradation and pollution such as the German AQEM index, while IBI 12 and Renkonen's similarity more reflect general community shifts caused by any type of disturbance. The new assessment system will be proposed for future use by state agencies for nationwide monitoring in the context of the Water Framework Directive implementation.  相似文献   

Conceptual study — An improved method to assess acidification in German streams by using benthic macroinvertebrates     

Ulrich Braukmann  Regina Biss 《Limnologica》2004,34(4):433-450

The present study is based mainly upon the authors' hydrochemical and hydrobiological studies of small silicate streams in South-West Germany, principally in the Black Forest and the Odenwald.

The aim of the paper is the adaptation of a practically proven four-level biological classification system of different degrees of acidity using benthic maroinvertebrates to a five-level system of acidification of mainly upland streams with low buffer capacity. The main reason for such a five-class system is the Water Framework Directive of the European Union (EU WFD), which lays down a five-level classification system for the assessment of the ecological quality of waters.

The biological method of assessing the state of acidity for evaluation of the degree of anthropogenic acidification under the directive, and principles of the EU WFD, are also described. A list of 278 taxa of the macrozoobenthos has been reclassified from a four-level system to a five-level biological indication system, based upon the authors' scientific expertise and the latest references from literature of different acid sensitive areas in Germany.  相似文献   

Does Pumping Volume Affect the Concentration of Micropollutants in Groundwater Samples?          下载免费PDF全文

Inge H. van Driezum  Julia Derx  Ernis Saracevic  Alexander K.T. Kirschner  Regina Sommer  Andreas H. Farnleitner  Alfred Paul Blaschke 《Ground Water Monitoring & Remediation》2017,37(4):82-88

Information on concentrations of micropollutants (such as pharmaceuticals, pesticides, and industrial chemicals) in most highly dynamic riverbank filtration (RBF) systems is lacking, in contrast to data on standard chemical parameters. Sampling protocols have thus far been based on the stabilization of standard chemical parameters in relatively pristine environments. To determine whether groundwater samples for micropollutant analysis can be taken at a similar pumping volume as samples for testing standard chemical parameters in both environments, three groundwater monitoring wells in an RBF system were sampled at two points in time (after pumping of 3 well volumes and after pumping of 15 well volumes). Micropollutant concentrations were not significantly different between the two sampling points; therefore, appropriate samples can be drawn after pumping 3 well volumes. For a specific microbiological parameter (leucin incorporation), a statistically significant difference was found.  相似文献   

NanoSr – A New Carbonate Microanalytical Reference Material for In Situ Strontium Isotope Analysis     

Michael Weber  Federico Lugli  Bodo Hattendorf  Denis Scholz  Regina Mertz‐Kraus  Damien Guinoiseau  Klaus Peter Jochum 《Geostandards and Geoanalytical Research》2020,44(1):69-83

The in situ measurement of Sr isotopes in carbonates by MC‐ICP‐MS is limited by the availability of suitable microanalytical reference materials (RMs), which match the samples of interest. Whereas several well‐characterised carbonate reference materials for Sr mass fractions > 1000 µg g^?1 are available, there is a lack of well‐characterised carbonate microanalytical RMs with lower Sr mass fractions. Here, we present a new synthetic carbonate nanopowder RM with a Sr mass fraction of ca. 500 µg g^?1 suitable for microanalytical Sr isotope research (‘NanoSr’). NanoSr was analysed by both solution‐based and in situ techniques. Element mass fractions were determined using EPMA (Ca mass fraction), as well as laser ablation and solution ICP‐MS in different laboratories. The ⁸⁷Sr/⁸⁶Sr ratio was determined by well‐established bulk methods for Sr isotope measurements and is 0.70756 ± 0.00003 (2s). The Sr isotope microhomogeneity of the material was determined by LA‐MC‐ICP‐MS, which resulted in ⁸⁷Sr/⁸⁶Sr ratios of 0.70753 ± 0.00007 (2s) and 0.70757 ± 0.00006 (2s), respectively, in agreement with the solution data within uncertainties. Thus, this new reference material is well suited to monitor and correct microanalytical Sr isotope measurements of low‐Sr, low‐REE carbonate samples. NanoSr is available from the corresponding author.  相似文献   

Ground traces of artificial earth satellites     

Jack T. Kent  Harry R. Betz 《Celestial Mechanics and Dynamical Astronomy》1969,1(1):91-109

This paper considers the ground trace of an artificial earth satellite. It determines the effects of the trace caused by perturbations due to atmospheric drag, the oblateness of the earth, and the moon and the sun as a third body.The necessary mathematical relations giving these perturbations which are available in literature are utilized (Betz, 1967; Brouwer and Clemence, 1961; Brouwer and Hori, 1961; Danby, 1962; Escobal, 1965; Kentet al., 1963; Kozai, 1962). Those relations unavailable elsewhere are derived.The computation was done by programming in FORTRAN language and utilizing an IBM 360/65.Captain, USAF  相似文献