Protective effects of Ecklonia cava extract on the toxicity and oxidative stress induced by hair dye in in-vitro and in-vivo models     

OH Jae-Young  RYU Bo-Mi  YANG Hye-Won  KIM Eun-A  LEE Jung-Suck  JEON You-Jin 《海洋湖沼学报（英文）》2019,(3):909-917

Oxidative hair dyes containingρ-phenylenediamine(PPD)are reported to induce an allergic reaction by promoting oxidative stress when absorbed through the skin.Despite the associated risk,these hair dyes remain popular owing to their convenience and sharpness of color.This makes it important to minimize the cytotoxicity and oxidative stress induced by PPD-containing hair dyes.Ecklonia cava extract has been evaluated in different studies for its protective effects against external stress in fibroblasts and keratinocytes.Our study was aimed at using in-vitro and in-vivo models to investigate the extract’s effects on cytotoxicity of and oxidative stress induced by PPD-containing hair dyes.Analysis of CIEL*a*b*Color space was first used to determine the range of E.cava extract that would not interfere with the coloring ability of the dye upon addition.Subsequently,the set ranges of E.cava extract(5% and 7%)were added to the hair dye and their toxicity assessed by evaluating the viability of fibroblasts and keratinocytes.The effects on developmental phenotypes and induction of oxidative stress by hair dye were evaluated and compared with those of hair dyes containing different contents of E.cava extract using an in-vivo zebrafish model.Our results showed that E.cava extract in hair dye could significantly decrease the cytotoxicity and levels of oxidative stress caused by hair dyes containing PPD in both in-vitro and in-vivo models.These results suggest that the addition of 7% E.cava extract to 250μg/mL hair dye does not interfere with the coloring ability of the dye while showing significant protective eff ects against the hair dye.The study proposes that the use of E.cava extract as an adduct to hair dyes containing PPD reduces the cytotoxicity and oxidative stress induced by these hair dyes.  相似文献   

急性UVB辐射对粉叶马尾藻过氧化损伤和主要抗氧化酶的影响     

袁世鹏  唐学玺  于琪  臧宇  李泽民  周斌 《海洋与湖沼》2018,49(4):821-828

粉叶马尾藻(Sargassum glaucescens)是台湾地区潮间带的重要大型藻类,其对紫外线增强的生理响应特征尚不清楚。本研究关注粉叶马尾藻在急性紫外线辐射(Ultraviolet radiation B,UVB)下的生理指标变化,探索了粉叶马尾藻在急性UVB辐照下抗氧化系统的瞬时反应过程。结果表明:低强度组[0.5W/(m2·s)]藻体在24h内未出现显著氧化损伤和蛋白质含量变化,但是细胞活力在辐照12h后显著降低,藻体超氧化物歧化酶(SOD)酶活性随辐照时间延长而降低,过氧化氢酶(CAT)酶活性呈现升高后下降的趋势,抗坏血酸过氧化物酶(APX)酶活性没有出现显著降低;高强度组[2W/(m2·s)]藻体在辐照3h后藻体出现明显氧化损伤,6h后细胞活力和蛋白质含量显著降低,藻体SOD酶活性随辐照时间延长而降低,CAT和APX酶活性呈现升高后降低的趋势,辐射12h后即显著降低。低强度UVB辐射对粉叶马尾藻的影响相对较小,表明粉叶马尾藻对UVB辐射具有一定耐受性;而高强度UVB辐射的影响较为显著,意味着UVB辐射的持续增强将超出粉叶马尾藻的耐受限度,进而造成不利影响。本研究结果初步揭示了粉叶马尾藻对于急性UVB辐射的增强的生理响应特征,为进一步研究UVB辐射增强的生态学效应提供参考依据。  相似文献   

A preliminary study of the variation of phytoplankton absorption coefficients in the northern South China Sea     

WUJingyu  HONGHuasheng  SHANGShaoling  DAIMinhan  LEEZhongping  LIShaojing 《海洋学报(英文版)》2009,28(5):17-29

The temporal and spatial variabilities of phytoplankton absorption coefficients (a ph (λ)) and their relationships with physical processes in the northern South China Sea were examined, based on in situ data collected from two cruise surveys during May 14 to 25, 2001 and November 2 to 21, 2002. Significant changes in the surface water in a ph values and B/R ratios (a ph (440)/a ph (675)) were observed in May, which were caused by a phytoplankton bloom on the inner shelf stimulated by a large river plume due...  相似文献   

Multivariate analysis of .uorescence and source identi. cation of dissolved organic matter in Jiaozhou Bay, China     

JIANG Fenghu  YANG Baijuan  LEE Frank Sen-Chun.  WANG Xiaoru  CAO Xuan 《海洋学报(英文版)》2009,(2):60-72

Hierarchical clustering analysis and principal component analysis (PCA) methods were used to assess the similarities and dissimilarities of the entire Excitation-emission matrix spectroscopy (EEMs) data sets of samples collected from Jiaozhou Bay, China. The results demonstrate that multivariate analysis facilitates the complex data treatment and spectral sorting processes, and also enhances the probability to reveal otherwise hidden information concerning the chemical characteristics of the dissolved organic matter (DOM). The distribution of di.erent water samples as revealed by multivariate results has been used to track the movement of DOM material in the study area, and the interpretation is supported by the results obtained from the numerical simulation model of substance tracing technique, which show that the substance discharged by Haibo River can be distributed in Jiaozhou Bay.  相似文献   

Bubble Size Distribution for Waves Propagating over A Submerged Breakwater     

TIEN Tsung-mo HUANG Ching-jer HSU Tai-wen LEE Chien-hsun HSU Shi-sheng 《中国海洋工程》2008,22(2):227-240

Experiments are carried out to study the characteristics of active bubbles entrained by breaking waves as these propagate over an abruptly topographical change or a submerged breakwater. Underwater sounds generated by the entrained air bubbles are detected by a hydrophone connected to a charge amplifier and a data acquisition system. The size distribution of the bubbles is then determined inversely from the received sound frequencies. The sound signals are converted from time domain to time-frequency domain by applying Gabor transform. The number of bubbles with different sizes are counted from the signal peaks in the time-frequency domain. The characteristics of the bubbles are in terms of bubble size spectra, which account for the variation in bubble probability density related to the bubble radius r. The experimental data demonstrate that the bubble probability density function shows a - 2.39 power-law sealing with radius for r 〉 0. 8 mm, and a- 1.11 power law for r 〈0.8 mm.  相似文献   

Identifying Indicator Species in Habitats Created by Coastal Structures   总被引：1，自引：1，他引：0  

Chun-Han SHIH  Ying-Chou LEE  Wei-Tse CHANG  Ta-Jen CHU 《中国海洋工程》2010,24(1):117-134

In this paper,three approachs were developed to find indicator species from the habitat created by coastal structures.These approachs consist of a model of species co-occurrence probability,a model of k-environmental factor probability and a composite model.Simultaneously,a case study was conducted in Hsinchu Fishing Port of north-western Taiwan.Based on the aforementioned models,three primary producer species,Ahnfeltiopsis flabelliformis,Chondrus ocellatus and Sarcodia montagneana,were chosen as the indicator species which had the highest co-occurrence probabilities and showed greater tolerance to more critical environment.It is imperative to understand how the three species under particular co-occurring conditions and environmental factors influence the composition of sessile assemblages in coastal water.The results indicate that for the purpose of increasing biodiversity,these models are feasible to find indicator species of artificial structures,and to help make it possible to design coastal structures based on biological considerations.This study provides an innovative approach for further advanced application in the artificial habitat of coast management.  相似文献