Design of Vibrio 16S rRNA Gene Specific Primers and Their Application in the Analysis of Seawater Vibrio Community     

LIU Yong YANG Guanpin WANG Hualei CHEN Jixiang SHI Xianming ZOU Guiwei WEI Qiwei SUN Xiuqin 《中国海洋大学学报(英文版)》2006,5(2):157-164

The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.  相似文献   

Meso‐scale particle modeling of concrete deterioration caused by alkali‐aggregate reaction     

Jianwen Pan  Y. T. Feng  Feng Jin  Yanjie Xu  Qicheng Sun  Chuhan Zhang  D. R. J. Owen 《国际地质力学数值与分析法杂志》2013,37(16):2690-2705

A meso‐scale particle model is presented to simulate the expansion of concrete subjected to alkali‐aggregate reaction (AAR) and to analyze the AAR‐induced degradation of the mechanical properties. It is the first attempt to evaluate the deterioration mechanism due to AAR using the discrete‐element method. A three‐phase meso‐scale model for concrete composed of aggregates, mortar and the interface is established with the combination of a pre‐processing approach and the particle flow code, PFC2D. A homogeneous aggregate expansion approach is applied to model the AAR expansion. Uniaxial compression tests are conducted for the AAR‐affected concrete to examine the effects on the mechanical properties. Two specimens with different aggregate sizes are analyzed to consider the effects of aggregate size on AAR. The results show that the meso‐scale particle model is valid to predict the expansion and the internal micro‐cracking patterns caused by AAR. The two different specimens exhibit similar behavior. The Young's modulus and compressive strength are significantly reduced with the increase of AAR expansion. The shape of the stress–strain curves obtained from the compression tests clearly reflects the influence of internal micro‐cracks: an increased nonlinearity before the peak loading and a more gradual softening for more severely affected specimens. Similar macroscopic failure patterns of the specimens under compression are observed in terms of diagonal macroscopic cracks splitting the specimen into several triangular pieces, whereas localized micro‐cracks forming in slightly affected specimens are different from branching and diffusing cracks in severely affected ones, demonstrating different failure mechanisms. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

Characteristics and distribution of clay minerals and their effects on reservoir quality: Huagang Formation in the Xihu Sag,East China Sea Basin     

X. L. Sun  X. G. Zhang  J. L. Lin  Z. X. Zhao  D. W. Huang 《Australian Journal of Earth Sciences》2013,60(8):1163-1174

Abstract

The characteristics and distribution of clay minerals and their effects on reservoir quality in the Huagang sandstones in the Xihu Sag, East China Sea Basin were studied by using X-ray diffraction, casting thin-sections, scanning electron microscopy, electron microprobe analysis, fluid inclusion analysis, constant-rate mercury injection and nuclear magnetic resonance. Clay minerals consist of kaolinite, chlorite, illite and illite–smectite mixed layer (I/S); kaolinite forms from dissolved feldspars, chlorite occurs as clay coatings that are transformed from clay precursors owing to the flocculation of suspended detrital clays or the crystallisation of pore fluids, and illite forms from the illitisation of detrital smectite, authigenic kaolinite and K-feldspars. Clay distribution is controlled by sedimentary environments, burial history and lithologies. Typical reservoirs in the western sub-sag are thin and developed in braided river facies at relatively shallow burial depths with clays dominated by kaolinite. However, typical reservoirs in the central inversion tectonic zone are thicker and developed in a braided delta front facies at deeper burial depths with clays mainly consisting of chlorite, illite and I/S. High-quality reservoirs are characterised by coarse granularity, high quartz content and low clay content with widespread development of chlorite coatings that inhibit quartz cements at low temperatures. At higher temperatures, the high-quality reservoirs develop more pores providing growth space for quartz cements and result in the coexistence of chlorite coatings and quartz cements. The high-quality reservoirs are controlled by their lithological characteristics rather than chlorite coatings. Illite and I/S clays create severe damage to reservoirs by reducing the size and connectivity of pore-throats.  相似文献   

Numerical simulation of ductile shear localization: Plastic‐flow network and slip‐line field     

Sheng‐zu Wang  Jun‐xiu Sun  Jie‐yuan Ning 《国际地质力学数值与分析法杂志》2008,32(3):307-324

  相似文献   

Geochemistry and spatial distribution of late-Paleozoic mafic volcanic rocks in the surrounding areas of the Gonghe Basin: Implications for Majixueshan triple-junction and east Paleotethyan archipelagic ocean     

Guo  AnLin  Zhang  GuoWei  Sun  YanGui  Cheng  ShunYou  Yao  AnPing 《中国科学:地球科学(英文版)》2007,50(2):292-304

The late-Paleozoic mafic volcanic rocks occurring in the surrounding areas of the Gonghe basin are distributed in the A’nyêmaqên ophiolite zone, Zongwulong tectonic zone and Kuhai-Saishitang volcanic zone. The mafic volcanics in the A’nyêmaqên zone formed an ancient ridge-centered hotspot around the Majixueshan OIB, the Kuhai-Saishitang mafic rocks consist of E-MORB and continental rift basalts and the Zongwulong volcanic rocks are enriched N-MORB. The regionally low Nb/U and Ce/Pb ratios reflect the influence of the OIB material on the mafic magma source. From geochemistry, spatial distribution and tectonic relationship of the mafic rocks, an ancient triple-junction centered at the Majixueshan can be inferred. The existence of the Kuhai-Saishitang aulacogen may have provided a tectonic channel for the Majixueshan OIB materials metasomatizing the magma source for the Zongwulong rocks. The formation of the triple-junction and the rifting of the Zongwulong zone have separated the orogens and massifs in the region.

  相似文献   

Estimation of the Roll Hydrodynamic Moment Model of a Ship by Using the System Identification Method and the Free Running Model Test     

Hyeon Kyu Yoon Nam Sun Son Gyeong Joong Lee 《Oceanic Engineering, IEEE Journal of》2007,32(4):798-806

When a fast container ship or a naval vessel turns, accompanying roll motions occur. This roll effect must be considered in the horizontal equations of the motion of the ship to predict the maneuverability of the ship properly. In this paper, a new method for determining a model structure of the hydrodynamic roll moment acting on a ship and for estimating the hydrodynamic coefficients is proposed. The method utilizes a system identification technique with the data from sea trial tests or from free running model (FRM) tests. To obtain motion data that is applied to the proposed algorithm, an FRM of a large container ship was developed. Using this model ship, standard maneuvering tests were carried out on a small body of water out of doors. A hydrodynamic roll moment model was constructed utilizing the data from turning circle tests and a 20-20 zig-zag test. This was then confirmed through a 10-10 zig-zag test. It was concluded that a model structure of the hydrodynamic roll moment model could be established without difficulty through a system identification method and FRM tests.  相似文献   

Describing neutron stars by the relativistic mean field theory     

Huan-yu Jia  Bao-xi Sun  Jie Meng  En-guang Zhao 《Chinese Astronomy and Astrophysics》2002,26(4):901-413

Neutron stars are studied in the framework of the relativistic mean field theory of interacting nucleons, hyperons, and mesons. Within the hadronic freedom, the cores of neutron stars are found to be dominated by hyperons when the density is sufficiently high. The influence of hyperon coupling constants on the transition from a neutron star to a hyperon-dominated strange neutron star is also investigated. It is found that the transition density gets its minimum value when the ratio of hyperon coupling constant to nucleon's takes the value of 0.65, and the calculated maximum mass of the neutron star is 1.4 M which lies within the range of the observational results.  相似文献   

长江三峡库区地震地形变监测研究   总被引：8，自引：4，他引：4  

杜瑞林  邢灿飞  伍中华  李胜乐  乔学军  游新兆  王琪  孙少安  申重阳  凌模 《大地测量与地球动力学》2004,24(2):23-29

三峡地壳形变监测网络结合空间大地测量(GPS、InSAR)和传统大地测量的优点，在1998年以来的三峡工程建设期间和2003年水库首次蓄水中获取了大量监测成果。结果显示库区地壳运动的基本图像：垂直形变较为显著，主要来自因蓄水荷载而产生的形变，水平形变不明显．库区主要断层的活动性在蓄水期间有明显加强迹象。水准测量反映从1998年首期观测至2003年10月首次蓄水后的库区垂直位移．沉降范围较大；GPS给出了2003年蓄水前后库区的垂直位移，形变的范围主要集中在水库近岸区域。水准测量与GPS观测反应的垂直位移的量级基本一致，沉降的最大幅度在3～4cm左右。水准监测、GPS观测与重力监测结果有一定的对应性。从地壳形变监测与研究结果分析，近期负荷改变而诱发中强地震的可能性不大。  相似文献   

Mapping models for near-conservative systems with applications     

Ji-Lin Zhou  Yan-Ning Fu  Yi-Sui Sun 《Celestial Mechanics and Dynamical Astronomy》1994,60(4):471-487

A systematic method is presented to construct a mapping model for a near-conservative system, based on that of Hadjidemetriou for a Hamiltonian system [1]. The mapping we constructed preserves the basic features of the actual system. We apply this method to the forced oscillating system and to the 3 : 1 resonant grain motion in the presence of Poynting-Robertson drag.Supported by the National Natural Science Foundation of China.  相似文献   

Forced secondary resonances at the 3 : 1 commensurability     

Yi-Sui Sun  Ji-Lin Zhou  Tian-Ling Zhang  Chong-Qing Cheng 《Celestial Mechanics and Dynamical Astronomy》1994,59(4):313-325

For the 3 : 1 Jovian resonance problem, the time scales of the two degrees of freedom of the resonant Hamiltonian are well-separated [5]. With the adiabatic approximation, the solution for the fast oscillations can be found in terms of the slowly varying variables. Thus the rapidly oscillating terms in the slow oscillation equations can be treated as forced terms. We refer to the resonance between the forcing and intrinsic frequencies as a forced secondary one in this paper. We discuss the forced secondary resonances in asteroidal motion at the 3 : 1 commensurability by using Wisdom's method. The results show that the orbits situated originally near the resonance will leave the neighbourhood of resonance and tend to the separatrices and critical points for different energies, respectively. We have not found any stochastic web as expected in this case. Moreover, we study the problem of validity on the approximation of a system.The Project Supported by the National Natural Science Foundation of China.  相似文献