A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians   总被引：2，自引：0，他引：2  

Azumi K  Fujie M  Usami T  Miki Y  Satoh N 《Marine environmental research》2004,58(2-5):543-546

To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT.  相似文献   

利用密度和核磁共振测井分析Mallik 5L-38井的天然气水合物饱和度     

T．Takayama  陆敬安 《海洋地质》2006,(3):64-70

以前的研究表明密度和核磁共振测井数据可用来精确计算储层的天然气饱和度。传统的气体储层中开发的用于天然气饱和度计算的密度-磁共振法也可用于天然气水合物饱和度分析。在不使用复杂方程或假设的情况下，基于经验方程，密度-磁共振方法可得到精确的地层孔隙度、气体和（或）天然气水合物饱和度。本文通过密度-磁共振响应方法来识别Mallik 5L-38井所穿沉积剖面中的天然气水合物带。结果证明密度-磁共振方法可以计算经气体校正后的孔隙度和精确的天然气水合物饱和度。  相似文献   

太平洋板块与北美板块在晚第三纪的相对运动     

Harbe.  W 郑军 《海洋地质译丛》1991,(5):70-77

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白垩纪温度较冷的估价   总被引：1，自引：0，他引：1  

B.W.Sellwood张光威  刘健 《海洋地质前沿》1995,(8):17-19

白垩纪温度较冷的估价Ｂ．ｗ．Ｓｅｌｌｗｏｏｄ等有关白垩纪气候的概念部分基于详尽的地质证据，如不耐寒冷的古生物（如：珊瑚的分布比现在偏极地５－１５℃）和特殊气候条件的沉积相的广泛分布。这些资料的解释反映２１℃的等温线在纬度上至少向两极移动５°，全球的平...  相似文献   

Using benthic macrofauna to assess environmental quality of four intertidal mudflats in Hong Kong and Shenzhen Coast   总被引：4，自引：0，他引：4  

Cai Lizhe  Nora F. Y. Tam  Teresa W. Y. Wong  Ma Li  Gao Yang  Yuk-Shan Wong 《海洋学报(英文版)》2003,22(2):309-319

Abstract-Intertidal zone is a significant wetland between land and ocean. It plays an important rolein maintaining local ecological balance. Both Mai Po and Futian intertidal mudflats are located in Shen-zhen Bay and are important "refueling" point along the East Asian/Australian flyway of migratory birds.The environmental quality of Mai Po and Futian mudflats have aroused great concern due to rapid eco-nomic developments in Hong Kong and Shenzhen in recent decades. Microinfauna of Mai Po and Futianmudflats was investigated in December 2000 and the faunal data were used to assess their environmentalquality. Two other mudflats, namely Ma Wan Typhoon Shelter (a more disturbed area) and Luk Kengintertidal mudflat (a place with relatively less human disturbance), were also sampled for macroinfaunaand used as reference mudflats. Shannon-Weaver species diversity index (I_(sd)), biotic coefficient (C_b)and macrofaunal pollution index (I_(mp)) of the macrofauna community on four intertidal mudflats wereused to  相似文献   

1nm锰矿相的金属阳离子交换能力的研究   总被引：6，自引：3，他引：6  

钱江初  T.A.Mellin 《海洋学报》1996,18(4):56-62

人工合成的1nm锰矿相和铜、钴、镍等二价金属阳离子的交换实验表明,1nm锰矿相具有强烈的金属阳离子交换能力.正是这种金属阳离子的交换能力,使得大洋多金属结核大量地富集了铜、钴、镍等有价金属元素.1nm锰矿相对几种金属阳离子的交换的优先顺序为:Cu>Co>Zn≥Ni>Ca>Mg.多金属结核中镍的含量往往高于铜、钴、锌等元素,但在交换顺序中它却低于铜、钴、锌等,这种不一致性说明结核成矿后期的金属离子交换并非是有价金属结合进结核的唯一途径.多金属结核富含铜、钴、镍等有价金属可能是结核初始成矿和成矿后期金属离子交换等多种因素共同作用的结果.  相似文献   

Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay     

Taban IC  Bechmann RK  Torgrimsen S  Baussant T  Sanni S 《Marine environmental research》2004,58(2-5):701-705

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.  相似文献   

底部边界层颗粒态物质迁移特征的放射性核素分析方法     

李炎  G.W.Berger 《海洋科学》1992,16(4):66-68

利用颗粒态放射性核素携带的颗粒物历经过程信息,我们提出用泥沙扩散方程和颗粒态放射性核素扩散方程联解底部边界层颗粒态物质迁移参数的方法。分析实例的样品取自荷兰Waden Sea南部Balgzand潮滩(砂坪)和Mok湾潮滩(泥坪)的两个站位(BG1和Mok2)。示踪核素为~(234)Th,~(210)Pb和~(137)Cs,其放射性比度由r能谱测出。  相似文献   

渤海东部（华北地台）晚第四纪地层学与沉积环境     

Marss.  T 刘光威 《海洋地质前沿》1997,(3):12-14

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赤道太平洋的环流     

SP·Hayes  郭洪寿 《海洋预报》1988,(Z1)

一.引 言 赤道太平洋上层海洋环流的主要特征已被建立十多年了。1942年斯维尔德鲁普等人描述了中太平洋的表面流:往西流的北和南赤道流以及往东流的北赤道逆流。随后,南赤道逆流也被用表面动力高度图表示出来(Reia,1961),Cromwell等人(1954)发现了赤道潜流,从而对上述环流的描述进行了主要修正。Hisard和Rual(1970)发现了赤道潜流下面  相似文献