Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay     

Taban IC  Bechmann RK  Torgrimsen S  Baussant T  Sanni S 《Marine environmental research》2004,58(2-5):701-705

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.  相似文献   

渤海东部（华北地台）晚第四纪地层学与沉积环境     

Marss.  T 刘光威 《海洋地质前沿》1997,(3):12-14

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太平洋海温场与东亚温带气旋的关系   总被引：1，自引：1，他引：1  

战淑芸  林玉英 《海洋预报》1990,7(1):8-14

本文分析了太平洋海温场与东亚温带气旋之间的相关关系。结果表明:太平洋海温场与东亚温带气旋之间存在着明显的相互影响的关系,而且这种影响关系有很强的持续性。有些海域,例如黑潮区与气旋之间存在着交替影响的关系。对2月气旋与海温之间相互影响过程的初步分析表明,海温与气旋的相互关系是与西风带环流及海流等相关联的。  相似文献   

超过例外氧暴露时限的空气巡回潜水实验中若干生理功能的观察     

陈宝松  荆岩林  郑继昌 《海洋科学》1991,15(1):35-39

17名潜水员安全地进行了55人次超过美国海军例外氧暴露时限1.5—3倍的空气巡回潜水实验。生理功能观察和肺活量测定结果表明,在水深70m以浅,潜水员均保持了相当好的智力和操作能力,在连续3d 3倍氧暴露时限的巡潜后期,只有3人肺活量下降,且在次日测定时已见恢复,表明上述暴露是安全的。美国海军例外氧暴露时限中对于70m以浅空气暴露的相应规定显得保守。  相似文献   

西沙群岛海区表层海水温度的谱分析     

容志明  宗林植 《南海研究与开发》1991,(2):1-5

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Multi-level assessment of chronic toxicity of estuarine sediments with the amphipod Gammarus locusta: II. Organism and population-level endpoints     

Costa FO  Neuparth T  Correia AD  Costa MH 《Marine environmental research》2005,60(1):93-110

This study aimed to test the performance of the amphipod Gammarus locusta (L.) in chronic sediment toxicity tests. It constitutes part of a multi-level assessment of chronic toxicity of estuarine sediments, integrating organism and population-level endpoints with biochemical markers responses. Here we account for organism and population-level effects, while biomarker responses were reported in a companion article. Five moderately contaminated sediments from Sado and Tagus estuaries were tested, comprising 3 muddy and 2 sandy sediments. These sediments either did not show acute toxicity or were diluted with control sediment as much as required to remove acute toxicity. Subsequent chronic tests consisted of 28-day exposures with survival, individual growth and reproductive traits as endpoints. Two of the muddy sediments induced higher growth rates in the amphipods, and improved reproductive traits. This was understood to be a consequence of the amount of organic matter in the sediment, which was nutritionally beneficial to the amphipods, while concurrently decreasing contaminant bioavailability. Biomarker responses did not reveal toxicant-induced stress in amphipods exposed to these sediments. One of the sandy sediments was acutely toxic at 50% dilution, but in contrast stimulated amphipod growth when diluted 75%. This was presumed to be an indication of a hormetic response. Finally the two remaining contaminated sediments showed pronounced chronic toxicity, affecting survival and reproduction. The sex ratio of survivors was highly biased towards females, and offspring production was severely impaired. The particulars of the responses of this amphipod were examined, as well as strengths versus limitations of the sediment test. This study illustrates the utility of this chronic test for toxicity assessment of contaminated estuarine sediments, with potential application all along Atlantic Europe.  相似文献   

煤炭资源勘查误差理论研究     

林宣明 《福建地质》2008,27(1):57-72

对煤炭资源勘查资源量与客观物质量之间差值的误差理论探讨,提出误差由真值误差、系统误差和离差3个基本部分组成,其中真值误差是不可消除的观测误差、系统误差是控制网度所决定的准确度标准、而离差是由地质构造及矿床形态的多解性产生的资源量波动。在此基础上,构筑了一个关于误差的数学模型,最后应用误差理论对现行勘查工作中常见的错误进行分析,并就如何避免错误及减少误差提出了见解。  相似文献   

贵州中东部剩余重力异常与区域矿产分布关系   总被引：1，自引：0，他引：1  

王亮  张应文  龙超林  晏承志  张家德  王毅  海宇清 《物探与化探》2008,32(2):116-121,125

重点研究1:20万剩余重力异常,结合航磁、物性、地质等资料,初步圈定区内隐伏、半隐伏岩浆岩体,对研究与重力异常、岩体分布关系密切的矿产的时间、空间分布及其成因,有一定参考价值和指示意义。  相似文献   

黔西北威-赫-纳-织-水地区铅锌(铜)矿带上 重力异常反映的地质信息   总被引：4，自引：1，他引：3  

王亮  乔计花  龙超林 《物探与化探》2008,32(4):374-378

研究黔西北威宁—赫章—纳雍—织金—水城地区,铅锌(铜)矿带上分布的1∶20万区域重力异常特征,及其与地质、构造、岩体、化探等异常的对应规律及其关系,能对该区此类矿产的成矿规律、成矿部位和成矿特点的研究有所帮助,提出下一步找矿方向上的参考建议。  相似文献   

钢筋混凝土桩网复合地基沉降计算方法优选     

黄国林 《云南地质》2008,27(1):97-101

钢筋混凝土桩网复合地基是一种新的地基处理技术。本文对适用于钢筋混凝土桩网复合地基沉降计算的方法进行探讨。  相似文献