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81.
“2007.4.1”四川盆地强寒潮天气过程分析 总被引:1,自引:0,他引:1
利用T2131°×1°和欧洲中心实时资料,对2007年4月1~3日四川盆地区域性寒潮天气过程的环流背景及影响系统进行了分析,结果表明:500hPa乌拉尔山到贝加尔湖以东为宽广深厚的低压带,地面上巴湖附近为冷高压,此冷高压在500hPa脊前西北气流的引导下东移南压,是造成此次寒潮大风天气的主要原因;而且低空急流也对降温有一定的作用。 相似文献
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本文以某工程为例,介绍了直达波法测定整板基础砼强度的技术方法;其结果可避免以往回弹法测量结果离散度较大而引起的争议。 相似文献
85.
White Spot Syndrome Virus (WSSV) was purified from hemolymph of infected shrimp. After nucleic acid extraction from the purified
virus particles, EcoR I-digested fragments of the WSSV genome were cloned; three of these fragments were used as non-radioactive
probes labeled with DIG-11-dUTP. The probes hybridized in situ, with sections located in the nuclei of all WSSV-infected tissues.
The virus was detected in the gill, stomach, epidermis, and connective tissue and so on, but not detected in healthy shrimp
tissues and epithelial cells of hepatopancreatic tubules of diseased shrimp.
This work was supported by SRF for ROCS, SEM and the National 863 Project (Grant 819-Q-08) and Project under major State Basic
Research Development Program (Grant G1999012002). 相似文献
86.
克隆获得了与中国明对虾(Fenneropenaeus chinensis)C-type lectin 3同源的C-型凝集素LvLec2基因序列,并通过Real-time PCR研究了其在脂多糖(lipopolysaccharide,简称LPS)、灭活溶壁微球菌(Micrococcus lysodeikticus)和白斑综合症病毒(white spot syndrome virus,简称WSSV)刺激后的转录表达变化。结果表明,LvLec2编码157个氨基酸,含有1个糖识别结构域(carbohydrate recognition domain,简称CRD),其CRD结构域中具有决定糖结合特异性的基序"EPS"(Glu118-Pro119-Ser120)序列。系统进化树分析表明LvLec2与已发表的凡纳滨对虾(Litopenaeus vannamei)C-型凝集素亲缘较远。LPS、灭活溶壁微球菌和WSSV刺激后,LvLec2基因在肝胰脏中的转录表达有明显的变化但表达模式不同。LvLec2可能作为模式识别受体参与了对虾对病原的识别或防御。 相似文献
87.
阐述了如何利用 MATLAB软件处理观测数据。 MATLAB是目前在大学和研究机构广泛应用的一种数值计算及图形工具软件 ,它的特点是语法结构简明 ,数值计算高效 ,图形功能完备 ,特别适合非专业编程人员完成数值计算、科学试验数据处理等任务。给出了一个水准网无约束平差算例及其源程序 相似文献
88.
The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV
(white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection,
feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those
in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present
in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM
in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei
often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All
our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed
to be more favorable for WSSV infection in F. chinensis. 相似文献
89.
Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes. 相似文献
90.
常规的油气化探方法一般是应用烷烃类作为油气勘探的基本指标,但是烷烃类易受人类活动和生物活动的干扰,使常规的化探技术在油气勘探中一直受到限制。人们开始考虑采用直接的生物标记化合物来预测油气藏,如芳烃系列中的苯系化合物苯(benzene)、甲苯(toluene)、乙苯(ethylbenzene)、二甲苯(xylem),简称BTEX。国内外研究表明,BTEX基本不受人类活动和生物活动的干扰,对油气藏具有良好的指示作用。依据南海某区的实测资料,采用自主研发的油气化探可视化评价软件进行数据处理,剥离背景异常,圈出有效的BTEX异常,并与靶区地层剖面进行了对比,结果证实了BTEX对海域油气藏具有良好的指示作用。 相似文献