渤海沉积物中产甲烷途径及产甲烷菌群落特征     

许晓晴  陈烨  甄毓  米铁柱  李晶  刘昌岭 《海洋地质与第四纪地质》2022,(3):50-61

全球甲烷排放主要来源于厌氧环境中产甲烷菌的代谢活动。采集渤海典型站位沉积物样品,测定了沉积物中甲烷和硫酸盐含量。通过提供不同类型产甲烷底物进行培养,分析了各样品中甲烷产量;通过高通量测序,分析了产甲烷菌群落特征。结果表明,渤海沉积物中产甲烷途径以甲基营养型为主,同时具有H₂/CO₂还原型途径。同一站点随着深度的增加,甲烷产生量逐渐减少,产甲烷速率相应降低。古菌群落以Crenarchaeota、 Asgardaeota和Nanoarchaeota为主,产甲烷菌Ca.Methanofastidiosales占据优势地位。本研究为全面了解产甲烷菌在海洋生境中产甲烷过程提供了重要参考。  相似文献   

Behavior of different phosphorus species in suspended particulate matter in the Changjiang estuary   总被引：1，自引：0，他引：1  

何会军  陈洪涛  姚庆祯  秦延文  米铁柱  于志刚 《中国海洋湖沼学报》2009,27(4):859-868

Suspended particulate matter (SPM) collected in the Changjiang (Yangtze River) estuary in June 2006 was separated into five fractions via water elutriation: clay-very fine silt (<8 μm), fine silt (8–16 μm), medium silt (16–32 μm), coarse silt (32-63 μm) and sand (>63 μm). The SPM and fractionated particles were sequentially analyzed by a modified SEDEX sequential extraction method to obtain six species of phosphorus: exchangeable or loosely-sorbed P, organic P, Fe-bound P, authigenic P, detrital P and refractory P. The results indicated that all particulate phosphorus species except for detrital P were negatively correlated to particle size; a high detrital P content was found in coarse silt and very coarse silt. From the inside of the river mouth to the gate of the river mouth, organic P, Fe-bound P and refractory P in the suspended particles decreased and a higher amount of exchangeable P appeared around the gate of the river mouth. From the gate of the river mouth to the sea, exchangeable P and organic P in suspended particles increased distinctly. The total particulate P flux into the estuary from the Changjiang River was about 45.45×10⁸ μmol/s during sampling. Of this, about 8.27×10⁸ μmol/s was associated with the “truly suspended” fraction. The bio-available particulate P flux was about 13.58×10⁸ μmol/s. Of this, about 4.24×10⁸ μmol/s was transported by “truly suspended” particles.  相似文献   

分子生物学在微藻分类研究中的应用   总被引：3，自引：0，他引：3  

甄毓  于志刚  米铁柱 《中国海洋大学学报(自然科学版)》2006,36(6):875-878,986

通过与传统微藻分类方法的比较,说明微藻分子分类技术的产生与发展,并从线粒体DNA（mtDNA）、叶绿体DNA（cpDNA）和核基因组DNA（nDNA）3个方面列举了分子分类技术在微藻鉴定和生理生态方面的应用,着重说明了核基困组在藻类分子检测中的作用。并对微藻分子分类技术的问题与发展进行了总结与展望。  相似文献