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A two-dimensional HPLC system, tandemly coupled to an ICP-MS, has been used to study copper accumulation and turnover in the visceral complex cytosol of the gastropod, Littorina littorea. Animals were exposed for 8 weeks to NTA-buffered seawater containing stable isotopic 65Cu and then transferred to media containing stable isotopic 63Cu. The free ion activity of each isotope was maintained at 10(-11) M. Size exclusion (SE) HPLC showed Cu associated with haemocyanin (HC) and metallothionein-like (MT) proteins in two ligand pools with apparent molecular weights of >300 kDa and approximately 17 kDa, respectively. The MT pool was inducible by Cu, could assimilate the metal from both intrinsic and extrinsic sources and showed a higher rate of Cu accumulation and turnover than the HC pool. The induction of this pool also caused the sequestration and cytosolic redistribution of Zn, Cd, Pb, Mn and Co. Further fractionation of the MT pool by ion-exchange (IE) HPLC revealed that the Cu was associated with a single, major isoform of the protein that was Cu inducible and also bound trace quantities of Zn and Pb. A number of additional metal containing proteins were also resolved by IE. the most prominent of which also bound Pb, Mn and minor quantities of Zn. The significance of these findings in metal homeostasis and detoxification is discussed. 相似文献
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Three-dimensional kinematic depth migration of converted waves: application to the 2002 Molise aftershock sequence (southern Italy) 总被引:1,自引:0,他引:1
Diana Latorre Pasquale De Gori Claudio Chiarabba Alessandro Amato Jean Virieux Tony Monfret 《Geophysical Prospecting》2008,56(4):587-600
Migration techniques, currently used in seismic exploration, are still scarcely applied in earthquake seismology due to the poor source knowledge and sparse, irregular acquisition geometries. At the crustal scale, classical seismological studies often perform inversions based on the arrival time of primary phases (P- and S-waves) but seldom exploit other information included in seismic records. Here we show how migration techniques can be adapted to earthquake seismology for converted wave analysis. As an example, we used data recorded by a dense local seismic network during the 2002 Molise aftershock sequence. In October and November 2002, two moderate magnitude earthquakes struck the Molise region (southern Italy), followed by an aftershock sequence lasting for about one month. Local earthquake tomography has provided earthquake hypocenter locations and three-dimensional models of P and S velocity fields. Strong secondary signals have been detected between first-arrivals of P- and S-waves and identified as SP transmitted waves. In order to analyse these waves, we apply a prestack depth migration scheme based on the Kirchhoff summation technique. Since source parameters are unknown, seismograms are equalized and only kinematic aspects of the migration process are considered. Converted wave traveltimes are calculated in the three-dimensional (3D) tomographic models using a finite-difference eikonal solver and back ray tracing. In the migrated images, the area of dominant energy conversion corresponds to a strong seismic horizon that we interpreted as the top of the Apulia Carbonate Platform and whose geometry and position at depth is consistent with current structural models from existing commercial seismic profiles, gravimetric and well data. 相似文献
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Ecological status assessment in the lower Eo estuary (Spain). The challenge of habitat heterogeneity integration: a benthic perspective 总被引:2,自引:1,他引:1
The implementation of the European Water Framework Directive (WFD) requires evaluation of the ecological status (ES) of benthic communities in coastal and transitional water systems, and the intercalibration of assessment methodologies therefore becomes a research challenge. Our aim was to test the suitability of applying the M-AMBI index to assess the status of the Eo estuary (northern coast of Spain). Our results showed that M-AMBI was influenced by the natural variability of benthic communities, and presented an apparent dependence on habitat characteristics. Consequently, the definition of homogeneous areas in transitional water systems should be based on the salinity gradient combined with other factors. To achieve an accurate ES assessment, habitat-specific reference conditions should then be defined prior to the application of M-AMBI; this necessitates dividing an estuary into several sections, which may be classified as different ESs. From this perspective, a novel approach to integrate habitat heterogeneity in a global ES assessment was tested. 相似文献
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This study develops a lattice Boltzmann method (LBM) with a two-relaxation-time collision operator (LTRT) to solve saltwater intrusion problems. A directional-speed-of-sound (DSS) technique is introduced to take into account the hydraulic conductivity heterogeneity and discontinuity, as well as the velocity-dependent dispersion coefficient. The forcing terms in the LTRT model are customized in order to recover the density-dependent groundwater flow and mass transport equations. Using the LTRT with the squared DSS achieves at least second-order accuracy. The LTRT results are verified with Henry’s analytical solution as well as compared with several numerical examples and modified Henry problems that consider heterogeneous hydraulic conductivity and velocity-dependent dispersion. The numerical results show good agreement with the Henry analytical solution and with the numerical solutions obtained by other numerical methods. 相似文献
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Novoa S Chust G Sagarminaga Y Revilla M Borja A Franco J 《Marine pollution bulletin》2012,64(4):739-750
The implementation of water quality European Directives requires an intensification of water quality monitoring, within the limits of the Exclusive Economic Zone. Remote sensing technologies can provide a valuable tool for frequent, synoptic, water-quality observations, over large areas. The aim of this study is to assess the ecological status of Basque coastal water bodies using satellite imagery from MODIS sensor, together with optical and chlorophyll-ain situ measurements. Thus, sea surface satellite-derived chl-a algorithms, the OC3 M, OC5 and a Local empirical algorithm, were compared against in situ measurements using satellite in situ match-ups, 90th Percentile (P90) monthly values for the 2005-2010 period. The OC5 algorithm corresponded most accurately with in situ measurements performed in the area, hence, it was selected. A P90 chlorophyll-a map was created with this algorithm to apply the classification scheme required by the directives. The classification of water bodies, based upon satellite-derived chlorophyll-a, could improve considerably the assessment of water quality. 相似文献
118.
Heliana Teixeira Ángel Borja J. Ananda Ranasinghe Daniel M. Dauer Steven Degraer Antoine Grémare Roberto J. Llansó João C. Marques Anna Occhipinti-Ambrogi Rafael Sardá Ronald G. Velarde 《Marine pollution bulletin》2010,60(4):589-600
Benthic indices are typically developed independently by habitat, making their incorporation into large geographic scale assessments potentially problematic because of scaling inequities. A potential solution is to establish common scaling using expert best professional judgment (BPJ). To test if experts from different geographies agree on condition assessment, sixteen experts from four regions in USA and Europe were provided species-abundance data for twelve sites per region. They ranked samples from best to worst condition and classified samples into four condition (quality) categories. Site rankings were highly correlated among experts, regardless of whether they were assessing samples from their home region. There was also good agreement on condition category, though agreement was better for samples at extremes of the disturbance gradient. The absence of regional bias suggests that expert judgment is a viable means for establishing a uniform scale to calibrate indices consistently across geographic regions. 相似文献
119.
The use of benthic indicators in Europe: from the Water Framework Directive to the Marine Strategy Framework Directive 总被引:1,自引:0,他引:1
Van Hoey G Borja A Birchenough S Buhl-Mortensen L Degraer S Fleischer D Kerckhof F Magni P Muxika I Reiss H Schröder A Zettler ML 《Marine pollution bulletin》2010,60(12):2187-2196
The Water Framework Directive (WFD) and the Marine Strategy Framework Directive (MSFD) are the European umbrella regulations for water systems. It is a challenge for the scientific community to translate the principles of these directives into realistic and accurate approaches. The aim of this paper, conducted by the Benthos Ecology Working Group of ICES, is to describe how the principles have been translated, which were the challenges and best way forward. We have tackled the following principles: the ecosystem-based approach, the development of benthic indicators, the definition of ‘pristine’ or sustainable conditions, the detection of pressures and the development of monitoring programs. We concluded that testing and integrating the different approaches was facilitated during the WFD process, which led to further insights and improvements, which the MSFD can rely upon. Expert involvement in the entire implementation process proved to be of vital importance. 相似文献
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