Molecular identification of anisakid nematodes third stage larvae isolated from common squid (Todarodes pacificus) in Korea     

Eko Setyobudi  Chan-Hyeok Jeon  Kwangho Choi  Sung Il Lee  Chung Il Lee  Jeong-Ho Kim 《Ocean Science Journal》2013,48(2):197-205

The occurrence of Genus Anisakis nematode larvae in marine fishes and cephalopods is epidemiologically important because Anisakis simplex larval stage can cause a clinical disease in humans when infected hosts are consumed raw. Common squid (Todarodes pacificus) from Korean waters were investigated for anisakid nematodes infection during 2009～2011. In total, 1,556 larvae were collected from 615 common squids and 732 of them were subsequently identified by PCR-RFLP analysis of ITS rDNA. Depending on the sampling locations, the nematode larvae from common squid showed different prevalence, intensity and species distribution. A high prevalence (P) and mean intensity (MI) of infection were observed in the Yellow Sea (n = 250, P = 86.0%, MI = 5.99 larvae/host) and the southern sea of Korea (n = 126, P = 57.1%, MI = 3.36 larvae/host). Anisakis pegreffii was dominantly found in common squid from the southern sea (127/ 140, 90.7%) and the Yellow Sea (561/565, 98.9%). In contrast, the P and MI of infection were relatively low in the East Sea (n = 239, P = 8.37%, MI = 1.25 larvae/host). A. pegreffii was not found from the East Sea and 52.0% (13/25) of the nematodes were identified as A. simplex. Most of them were found in the body cavity or digestive tract of common squid, which are rarely consumed raw by humans. Considering the differenences in anisakid nematode species distribution and their microhabitat in common squid, it remains unclear whether common squid plays an important role in the epidemiology of human anisakis infection in Korea. Further extensive identification of anisakid nematodes in common squid, with geographical and seasonal information will be necessary.  相似文献   

Assessing distributed groundwater recharge rate using integrated surface water-groundwater modelling: application to Mihocheon watershed, South Korea   总被引：1，自引：1，他引：0  

Il-Moon Chung  Nam-Won Kim  Jeongwoo Lee  Marios Sophocleous 《Hydrogeology Journal》2010,18(5):1253-1264

A method of estimating groundwater recharge, based on water-balance components using the SWAT-MODFLOW model (an integrated surface water-groundwater model), is described. A multi-reservoir storage routing module is suggested instead of a single storage routing module in SWAT; this represents a more realistic delay in the travel of water through the vadose zone. By using this module, the parameter related to the delay time can be optimized by checking the correlation between simulated recharge and observed groundwater levels. The final step of this procedure is to compare simulated groundwater levels as well as the simulated watershed stream flow with the observed groundwater levels and watershed stream flow. This method is applied to the Mihocheon watershed in South Korea to estimate spatio-temporal groundwater recharge distribution. The computed annual recharge rate is compared with the independently estimated recharge rate using BFLOW. The hydrologic modelling results show that the annual average recharge rate should be estimated by a long-term continuous simulation with a distributed hydrologic modelling technique.  相似文献