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11.
Background
Reactive oxygen species (ROS) are vital regulators of many cellular functions in the body. The intracellular ROS concentration is highly regulated by a balance between pro-oxidants and anti-oxidants. A chronic excess of pro-oxidants leads to elevated ROS concentrations and inflammation, possibly initiating or enhancing disease onset. Mineral-induced generation of ROS, the role of minerals in upregulating cellular ROS, and their role in the development of several occupational diseases are now widely recognized. However, there is no standard protocol to determine changes in ROS production in cells after exposure to mineral dust or earth materials in general. In this study, a new method for determining the degree of cellular toxicity (i.e., cytotoxicity) of particles is described that will help bridge the gap in knowledge.Results
By measuring the production of ROS and the viability of cells, an inflammatory stress response (ISR) indicator is defined. This approach normalizes the ROS upregulation with respect to the number of viable cells at the time of measurement. We conducted experiments on a series of minerals and soils that represent materials that are inert (i.e., glass beads, anatase, and a soil with low trace element content), moderately reactive (i.e., soil with high trace element content), and highly reactive (i.e., pyrite). Inert materials generated the lowest ISR, averaging 350% compared to the control. Acid washed pyrite produced the highest ISR (1,100 fold higher than the control). The measurements conducted as a function of time showed a complex response. Most materials showed an increase in ISR with particle loading.Conclusions
The amount of cellularly generated ROS and cell viability combined provide a better understanding of particle-induced oxidative stress. The results indicate that some earth materials may solicit an initial burst of ROS, followed by a second phase in which cell viability decreases and ROS production increases, leading to a high ISR value. Hence, measurements conducted over a range of particle loading combined with multiple data measurements up to 24 hours can provide new insights in the possible effect of exposure to earth materials on human health. 相似文献12.
The abundant iron sulfide mineral pyrite has been shown to catalytically produce hydrogen peroxide (H2O2) and hydroxyl radical ( . OH) in slurries of oxygenated water. Understanding the formation and fate of these reactive oxygen species is important to biological and ecological systems as exposure can lead to deleterious health effects, but also environmental engineering during the optimization of remediation approaches for possible treatment of contaminated waste streams. This study presents the use of the amino acid phenylalanine (Phe) to monitor the kinetics of pyrite-induced . OH formation through rates of hydroxylation forming three isomers of tyrosine (Tyr) - ortho-, meta-, and para-Tyr. Results indicate that about 50% of the Phe loss results in Tyr formation, and that these products further react with . OH at rates comparable to Phe. The overall loss of Phe appeared to be pseudo first-order in [Phe] as a function of time, but for the first time it is shown that initial rates were much less than first-order as a function of initial substrate concentration, [Phe]o. These results can be rationalized by considering that the effective concentration of . OH in solution is lower at a higher level of reactant and that an increasing fraction of . OH is consumed by Phe-degradation products as a function of time. A simplified first-order model was created to describe Phe loss in pyrite slurries which incorporates the [Phe]o, a first-order dependence on pyrite surface area, the assumption that all Phe degradation products compete equally for the limited supply of highly reactive . OH, and a flux that is related to the release of H2O2 from the pyrite surface (a result of the incomplete reduction of oxygen at the pyrite surface). An empirically derived rate constant, K pyr , was introduced to describe a variable . OH-reactivity for different batches of pyrite. Both the simplified first-order kinetic model, and a more detailed numerical simulation, yielded results that compare well to the observed kinetic data describing the effects of variations in concentrations of both initial Phe and pyrite. This work supports the use of Phe as a useful probe to assess the formation of . OH in the presence of pyrite, and its possible utility for similar applications with other minerals. 相似文献
13.
Katarina Gobo Massimiliano Ghinassi Wojciech Nemec Eivind Sjursen 《Sedimentology》2014,61(4):1086-1119
This study from the southern margin of the Gulf of Corinth documents a Late Pleistocene incised valley‐fill succession that differs from the existing facies models, because it comprises gravelly shoal‐water and Gilbert‐type deltaic deposits, shows strong wave influence and lacks evidence of tidal activity. The valley‐fill is at least 140 m thick, formed in 50 to 100 ka between the interglacials Marine Isotope Stage 9a and Marine Isotope Stage 7c. The relative sea‐level rise left its record both inside and outside the incised valley, and the age of the valley‐fill is estimated from a U/Th date of coral‐bearing deposits directly outside the palaeovalley outlet. Tectonic up‐warping due to formation of a valley‐parallel structural relay ramp contributed to the valley segmentation and limited the landward extent of marine invasions. The valley segment upstream of the ramp crest was filled with a gravelly alluvium, whereas the downstream segment accumulated fluvio‐deltaic deposits. The consecutive deltaic systems nucleated in the ramp‐crest zone, forming a bathymetric gradient that promoted the ultimate growth of thick Gilbert‐type delta. The case study contributes to the spectrum of conceptual models for incised valley‐fill architecture. Four key models are discussed with reference to the rates of sediment supply and accommodation development, and it is pointed out that not only similarity, but also all departures of particular field cases from these end‐member models may provide valuable information on the system formative conditions. The Akrata incised valley‐fill represents conditions of high sediment supply and a rapid, but stepwise development of accommodation that resulted from the spatiotemporal evolution of normal faulting at the rift margin and overprinted glacioeustatic signals. This study adds to an understanding of valley‐fill architecture and provides new insights into the Pleistocene tectonics and palaeogeography of the Corinth Rift margin. 相似文献
14.
Corey A Cohn Steffen Mueller Eckard Wimmer Nicole Leifer Steven Greenbaum Daniel R Strongin Martin AA Schoonen 《Geochemical transactions》2006,7(1):3-11
Background
Pyrite, the most abundant metal sulphide on Earth, is known to spontaneously form hydrogen peroxide when exposed to water. In this study the hypothesis that pyrite-induced hydrogen peroxide is transformed to hydroxyl radicals is tested. 相似文献15.
The occurrence of infections with Trichinella sp. in polar bears (Ursus maritimus) from northeastern Greenland has been studied by examination of muscle samples, mainly diaphragm, from 38 animals shot during the period 1983-1987. Trichinella larvae were demonstrated in 12 bears (32%) with an average of 9.2 larvae/g muscle tissue. No bears younger than three years old were infected. The prevalence of Trichinella among bears of the age group 3-4 years was 25% and 53% among older animals. 相似文献
16.
Gilbert‐type deltas recording short‐term base‐level changes: Delta‐brink morphodynamics and related foreset facies 
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下载免费PDF全文 Gilbert‐type deltas are sensitive recorders of short‐term base‐level changes, but the delta‐front record of a base‐level rise tends to be erased by fluvial erosion during a subsequent base‐level fall, which renders the bulk record of base‐level changes difficult to decipher from the delta‐front deposits. The present detailed study of three large Pleistocene Gilbert‐type deltas uplifted on the southern coast of the Gulf of Corinth, Greece, indicates a genetic link between the delta‐front morphodynamic responses to base‐level changes and the delta‐slope sedimentation processes. Sigmoidal delta‐brink architecture signifies a base‐level rise and is accompanied by a debrite‐dominated assemblage of delta foreset deposits, thought to form when the aggrading delta front stores sediment and undergoes discrete gravitational collapses. Oblique delta‐brink architecture tends to be accompanied by a turbidite‐dominated assemblage of foreset deposits, which are thought to form when the delta‐front accommodation decreases and the sediment carried by hyperpycnal effluent bypasses the front. This primary signal of the system response to base‐level changes combines further with the secondary ‘noise’ of delta autogenic variation and possible allogenic fluctuations in fluvial discharge due to regional climatic conditions. Nevertheless, the evidence suggests that the facies trends of delta foreset deposits may be used to decipher the delta ‘hidden’ record of base‐level changes obliterated by fluvial topset erosion. Early‐stage bayhead deltas may be an exception from the hypothetical model, because their narrow front tends to be swept by river floods irrespective of base‐level behaviour and their subaqueous slope deposits are thus mainly turbidites. 相似文献
17.
The paper explores the impact of hydrology on the Port-Harcourt–Patani-Warri Road. It reviews the hydrometeorological, drainage
and terrain peculiarities of the area against the backdrop of the design, alignment and performance of the road. The paper
ascribes the poor performance of the road to:
1. The southeast-northwest orientation of the road in a region with predominately northeast-southwest surface and subsurface
flow, in which the road acts like a dam;
2. The inferior construction aggregate composition;
3. Changes in pavement condition due to interaction of local road aggregates with water.
Received: 21 September 1998 · Accepted: 16 November 1999 相似文献