Biodegradation of <Emphasis Type="Italic">Enteromorpha</Emphasis> polysaccharides by intestinal micro-community from <Emphasis Type="Italic">Siganus oramin</Emphasis>     

Zhibiao Zhang  Xuefeng Han  Yan Xu  Jin Li  Yuanyou Li  Zhong Hu 《中国海洋大学学报(英文版)》2016,15(6):1034-1038

Micro-communities are supposed to have more potential functions of biodegradation of polysaccharides than single strain; however, the intestinal micro-communities involved in the biodegradation of Enteromorpha polysaccharides (EP) were seldom reported. In order to obtain the EP-degrading micro-community, the intestines of Siganus oramin was obtained to isolate the micro-communities, which were enriched by 0.3% of EP as the sole carbon source. A stable micro-community with EP degradative capability was achieved after seven generations of subculture, named H1. Results showed that H1 was able to degrade 75% of EP within 24 hours, and the activity of EP lyases reached 500 U mL^?1 in 32 hours. With denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone library analysis, ten bacteria closely related to Marinomonas pontica, Microbacterium sp., Leucobacter chironomi, Cyclobacterium sp., Algoriphagus winogradskyi, Pseudoalteromonas sp. and Vibrio sp. were determined. Furthermore, compared with the DGGE bands sequence and the clone library analysis, the dominant bacteria of the EP-biodegrading micro- community were Pseudoalteromonas sp. and Vibrio sp., with the respective proportion of 38% and 46%, and they should play an important role in EP degradation together with other degrading bacteria in the micro-community H1.  相似文献   

Antialgal and antilarval activities of bioactive compounds extracted from the marine dinoflagellate <Emphasis Type="Italic">Amphidinium carterae</Emphasis>     

Xianyu Kong  Xiurong Han  Min Gao  Rongguo Su  Ke Wang  Xuzhao Li  Wei Lu 《中国海洋大学学报(英文版)》2016,15(6):1014-1020

With the global ban on the application of organotin-based marine coatings by the International Maritime Organization, the development of environmentally friendly, low-toxic and nontoxic antifouling compounds for marine industries has become an urgent need. Marine microorganisms have been considered as a potential source of natural antifoulants. In this study, the antifouling potential of marine dinoflagellate Amphidinium carterae, the toxic and red-tide microalgae, was investigated. We performed a series of operations to extract the bioactive substances from Amphidinium carterae and tested their antialgal and antilarval activities. The crude extract of Amphidinium carterae showed significant antialgal activity and the EC₅₀ value against Skeletonema costatum was 55.4 μg mL^?1. After purification, the isolated bioactive substances (the organic extract C) exhibited much higher antialgal and antilarval activities with EC₅₀ of 12.9 μg mL^?1 against Skeletonema costatum and LC₅₀ of 15.1 μg mL^?1 against Amphibalanus amphitrite larvae. Subsequently, IR, Q-TOFMS, and GC-MS were utilized for the structural elucidation of the bioactive compounds, and a series of unsaturated and saturated 16- to 22-carbon fatty acids were detected. The data suggested the bioactive compounds isolated from Amphidinium carterae exhibited a significant inhibiting effect against the diatom Skeletonema costatum and Amphibalanus amphitrite larvae, and could be substitutes for persistent, toxic antifouling compounds.  相似文献   

Enhanced bioremoval of refractory compounds from dyeing wastewater using optimized sequential anaerobic/aerobic process     

W. Bae  D. Han  E. Kim  R. A. de Toledo  K. Kwon  H. Shim 《International Journal of Environmental Science and Technology》2016,13(7):1675-1684

The upflow anaerobic sludge blanket process followed by the biological aerated filter process was employed to improve the removal of color and recalcitrant compounds from real dyeing wastewater. The highest removal efficiency for color was observed in the anaerobic process, at 8-h hydraulic retention time, seeded with the sludge granule. In the subsequent aerobic process packed with the microbe-immobilized polyethylene glycol media, the removal efficiency for chemical oxygen demand increased significantly to 75 %, regardless of the empty bed contact time. The average influent non-biodegradable soluble chemical oxygen demand was 517 mg/L, and the average concentration in effluent from the anaerobic reactor was 363 mg/L, suggesting the removal of some recalcitrant matters together with the degradable ones. The average non-biodegradable soluble chemical oxygen demand in effluent from the aerobic reactor was 87, 93, and 118 mg/L, with the removal efficiency of 76, 74, and 67 %, at 24-, 12-, and 8-h empty bed contact time, respectively. The combined anaerobic sludge blanket and aerobic cell-entrapped process was effective to remove the refractory compounds from real dyeing wastewater as well as in reducing organic loading to meet the effluent discharge limits. This integrated process is considered an effective and economical treatment technology for dyeing wastewater.  相似文献   

New variogram modeling method using MGGP and SVR     

Changik Han  Jiyang Wang  Mingguo Zheng  Ende Wang  Jianming Xia  GwangSu Li  Sunchol Choe 《Earth Science Informatics》2016,9(2):197-213

A critical step for kriging in geostatistics is estimation of the variogram. Traditional variogram modeling comprise of the experimental variogram calculation, appropriate variogram model selection and model parameter determination. Selecting of the variogram model and fitting of model parameters is the most controversial aspect of geostatistics. Shapes of valid variogram models are finite, and sometimes, the optimal shape of the model can not be fitted, leading to reduced estimation accuracy. In this paper, a new method is presented to automatically construct a model shape and fit model parameters to experimental variograms using Support Vector Regression (SVR) and Multi-Gene Genetic Programming (MGGP). The proposed method does not require the selection of a variogram model and can directly provide the model shape and parameters of the optimal variogram. The validity of the proposed method is demonstrated in a number of cases.  相似文献   

Grain-size distribution and contamination characteristics of heavy metal in street dust of Baotou,China   总被引：1，自引：0，他引：1  

Xiufeng Han  Xinwei Lu  Qinghui Zhang  Wuyuntana  Quansheng Hai  Huiyun Pan 《Environmental Earth Sciences》2016,75(6):468

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A study of surface mixing in a meandering channel using GPS floaters     

Eun Jin Han  Inhwan Park  Young Do Kim  Il Won Seo 《Environmental Earth Sciences》2016,75(10):901

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Identifying structure and function of karst aquifer system using multiple field methods in karst trough valley area,South China   总被引：1，自引：0，他引：1  

Mingming Luo  Zhihua Chen  Hong Zhou  Hamza Jakada  Liang Zhang  Zhaofeng Han  Tingting Shi 《Environmental Earth Sciences》2016,75(9):824

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Investigation of the impacts of local-scale hydrogeologic conditions on sinkhole occurrence in East-Central Florida,USA     

Han Xiao  Yong Je Kim  Boo Hyun Nam  Dingbao Wang 《Environmental Earth Sciences》2016,75(18):1274

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Quantifying the hazardous impacts of human-induced land degradation on terrestrial ecosystems: a case study of karst areas of south China     

Degen Lin  Han Yu  Fang Lian  Jing-ai Wang  A-xing Zhu  Yaojie Yue 《Environmental Earth Sciences》2016,75(15):1127

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Analysis of phosphorus import characteristics of the upstream input rivers of Three Gorges Reservoir     

Chaonan Han  Binghui Zheng  Yanwen Qin  Yingqun Ma  Wei Cao  Chenchen Yang  Zhichao Liu 《Environmental Earth Sciences》2016,75(12):1024

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