海洋Pseudomonasaeruginosasp.DES-3的鉴定以及基因ap02对其产蛋白酶的影响研究          下载免费PDF全文

刘瑶  陈俊海  肖桂龙  孟灿  曾蓉  武波  蒋承建 《海洋科学进展》2016,34(4):553-559

海洋是地球上最大的生态系统,其多样独特的生态环境造就了其有别于其他环境的微生物资源。本研究利用蛋白酶的活性筛选策略,从辽宁省营口市某海洋淤泥中分离得到一株产蛋白酶的优势菌株DES-3。通过微生物形态学特征、生理生化性质和16SrDNA基因鉴定以及系统发育进化关系的分析,将该菌株鉴定为铜绿假单胞菌属,命名为Pseudomonas aeruginosa sp.DES-3。其胞外蛋白酶的最适反应温度和最适pH分别为55℃和9.0。将来自于碱性污染土壤宏基因组文库的编码碱性蛋白酶基因ap02与广宿主表达载体pBBR1MCS-5连接,转化至野生型菌株P.aeruginosasp.DES-3细胞中,成功构建了基因工程菌株P.aeruginosasp.DES-3/pBBR1MCS-5-ap02。基因工程菌株的最适反应温度为60℃,较野生型菌株提高了5℃。本研究构建基因工程菌株的策略可为菌株的改造和相应工业应用提供一定的技术参考。  相似文献   

Observed characteristics of atmospheric ducts over the South China Sea in autumn     

Cheng  Yinhe  Zhou  Shengqi  Wang  Dongxiao  Lu  Yuanzheng  Huang  Ke  Yao  Jinglong  You  Xiaobao 《中国海洋湖沼学报》2016,34(3):619-628

The observed characteristics of lower atmospheric ducts over the South China Sea(SCS) were analyzed based on Global Position Systerm(GPS) radiosonde data collected four times daily during autumn open cruises from 2006 to 2012.Duct occurrence,thickness,and strength over the SCS were about 40%,150-m thick,and 8 M units,respectively,which were larger than during the summer monsoon period.Most ducts occurred at heights 1 500 m and these ducts easily trap electromagnetic wave clusters with wavelengths 2 m.Diurnal variation of the SCS ducts appeared evident.They occurred more often at midnight at higher altitudes(about 1 100 m),with a thickest layer of about 145 m and less frequently during the evening at lower altitudes(about 800 m),with a thinnest layer of about 125 m.Moreover,ducts during the daytime at a mean height of about 900 m,with the greatest strength of about 10 M units.Furthermore,all duct variables observed over the SCS in autumn decreased from north to south.These findings are useful not only in the design of radar and communication systems,but also for evaluating possible effects of anomalous propagation on meteorological radar and military applications.  相似文献   

Refining the understanding of the mineral system using deep geoelectrical data     

Lanfang He  Xuefeng Zhao  Xiaolu Xu  Rujun Chen  Hongchun Yao 《《地质学报》英文版》2018,92(Z1):295-297

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基于Pasternak双参数地基模型的陡坡段桥梁基桩内力计算方法          下载免费PDF全文

赵明华  彭文哲  杨超炜  肖尧 《水文地质工程地质》2018,(1):52-59

为考虑土体剪切模量对陡坡段桥梁基桩内力计算的影响,首先探讨桩后土压力的分布形式,并根据陡坡段桥梁基桩的承载特性建立简化分析模型;其次,引入Pasternak双参数模型,建立可考虑土体剪切模量的地基抗力计算模型;并在此基础上分别对受荷段与嵌固段基桩微元进行受力分析,求得各段控制差分方程,从而进一步迭代求解桩身位移及内力;最后,分别用陡坡段桥梁基桩实测数据与Pasternak双参数模型算例对本文内力计算方法进行验算,结果表明:本文计算方法用于陡坡段桥梁基桩内力计算是合理的,可为同类工程提供参考。  相似文献   

山东黄金西岭勘查区深部勘探孔防斜与纠斜措施          下载免费PDF全文

潘垚 《探矿工程》2018,45(3):37-41

西岭勘查区金矿找矿工作已全面进入勘探阶段,勘查区网度为80 m×80 m,对于钻孔质量要求十分严格：终孔靶点偏差不超过勘探网度的四分之一,即不超过20 m。对于西岭勘查区设计孔深均超过1500 m（部分钻孔设计甚至超过2500 m）的钻孔而言,这种要求远远超过地质岩心钻探规程要求。山东黄金地勘公司根据多年深孔施工经验,在防斜与纠斜方面均取得了一些突破,自主研发了钻具扶正器、独创了钻孔纠斜法,形成了一套实用的钻孔质量保证措施。  相似文献   

The Fragmentation in a Protoplanetary Disk and the Properties of the Resultant Self-Gravitating Clumps     

Chunjian Liu  Zhen Yao  Lianhua Tian 《Astronomy Reports》2018,62(11):713-723

We study the fragmentation properties in the protoplanetary disk and properties of the resultant self-gravitating clumps using our newly constructed disk model. Our disk model includes the mass inflall term from a molecular cloud core and the photoevaporation winds effect. We adopt the conventional fragmentation criterion to judge whether a protoplanetary disk can fragment. In this work, we follow our previous work to investigate the properties of the resultant self-gravitating clumps. In our calculation, the initial masses of the resultant self-gravitating clumps lie in the range of tens of M_J to more than one hundred of M_J, where M_J is the Jupiter mass. These initial masses can seemingly account for the masses of extrasolar planets in magnitude. We also calculate the subsequent gas accretion of clumps in 1.27 × 10⁴ yr after the formation of self-gravitating clumps. We find that the subsequent gas accretion of self-gravitating clumps is very efficient, and the clump masses grow to hundreds of M_J and the physical radii R_c of clumps increase to about 10 AU. Additionally, we also calculate the orbital migration of clumps. We find that most clumps have short migration timescale to be accreted onto the protostar, and only a small fraction of clumps have long migration timescale (>10⁶ yr) to successfully become gas giant planets. These results are consistent with previous studies.  相似文献   

Characterizing groundwater/surface-water interactions in the interior of Jianghan Plain,central China     

Du  Yao  Ma  Teng  Deng  Yamin  Shen  Shuai  Lu  Zongjie 《Hydrogeology Journal》2018,26(4):1047-1059

Hydrogeology Journal - Quantifying groundwater/surface-water interactions is essential for managing water resources and revealing contaminant fate. There has been little concern on the exchange...  相似文献   

Correlation analysis between drought indices and terrestrial water storage from 2002 to 2015 in China     

Yajie Zhang  Yao Li  Jing Ge  Gaopeng Li  Zhisheng Yu  Haishan Niu 《Environmental Earth Sciences》2018,77(12):462

Drought monitoring is a key topic in environmental monitoring and assessment although there is still a need to determine the correlation between drought monitoring indices and remote sensing products. We analyzed the correlation between the self-calibrating Palmer Drought Severity Index (sc_PDSI), the Standardized Precipitation Index and the Standardized Precipitation Evapotranspiration Index (SPEI) and terrestrial water storage monitored through the Gravity Recovery and Climate Experiment (GRACE) on a monthly timescale from 2002 to 2015 in China. As a consequence of anomalies in the soil water budget, the highly significant correlation between the sc_PDSI and the GRACE satellite-observed terrestrial water storage suggested that these two datasets are the most suitable for use in monitoring droughts. In comparing the three drought indices, the sc_PDSI was introduced as a means of drought monitoring in the Yangtze, Pearl, Huaihe, Southeast and Songhua River Basins, whereas the SPEI was found to be more applicable to other major river basins, such as the Inland River Basin. These diverse spatial behaviors are caused by the differences between the hydrological droughts characterized by these three drought indices.  相似文献   

The influence of the Three Gorges Reservoir regulation on a typical tributary heat budget     

Yao Cheng  Yuchun Wang  Huaidong Zhou  Chenghua Dang 《Environmental Earth Sciences》2018,77(22):764

Reservoir regulation and local climate both affect the heat budget of tributary bay. It is difficult for traditional methods to identify the influence of different factors on heat budget quantitatively. In this paper, for analysis of the control mechanisms of the heat budget of a large reservoir tributary, the water temperature distribution, and heat budget processes of the Meixi River, a typical tributary to the Three Gorges Reservoir was measured, and a new method was used to calculate the heat content composition of the tributary bay and identify the key factor of the heat balance. The result shows significant variation in the spatial and temporal distributions of water temperatures in the Meixi River, ranging from 12.4 to 28.9 °C on the surface and 12.0 to 24.4 °C at the bottom. The total heat exchange across the air–water interface that ranges from 0.1 to 6% of the budget is not the primary control factor of the annual tributary heat budget. Rather, the change in water depth produced by regulation of the Three Gorges Reservoir is the primary control factor of the tributary heat budget in the whole year, which ranges from 72 to 99% of the budget. The water temperature difference between the main stream and tributary is the not key factor of the heat budget, which ranges from 0.1 to 28% of the heat budget.  相似文献   

Soil wettability in ground engineering: fundamentals,methods, and applications     

S. D. N. Lourenço  Y. Saulick  S. Zheng  H. Kang  D. Liu  H. Lin  T. Yao 《Acta Geotechnica》2018,13(1):1-14

Wettability is a fundamental property controlling the extent of wetting in flat and granular solids. In natural soils, wettability affects a wide variety of processes including infiltration, preferential flow and surface runoff. In mineral processing, wettability is paramount in enhancing the efficiency of separation of minerals from gangue. The manipulation of surface wettability is equally crucial in many industrial applications. For instance, superhydrophobic surfaces are those on which water drops roll off easily and as such are used for self-cleaning applications. Therefore, while wettability is strongly cross-disciplinary, its evolution has been discipline-specific with a direct extrapolation or transfer of concepts, approaches, and methods to ground engineering unlikely to remain valid. This paper synthesizes relevant aspects from surface chemistry, materials science, mining engineering, and soil science, and discusses their implications within the context of new granular materials that resist wetting, for use in barriers or ground improvement and, in unsaturated soils, where the effects of wettability have been documented.  相似文献