Protein carbonyls and antioxidant defenses in corkwing wrasse (Symphodus melops) from a heavy metal polluted and a PAH polluted site     

Almroth BC  Sturve J  Stephensen E  Holth TF  Förlin L 《Marine environmental research》2008,66(2):271-277

The use of fish in environmental monitoring has become increasingly important in recent years as anthropogenic substances, many of which function as prooxidants, are accumulating in aquatic environments. We have measured a battery of antioxidant defenses as a measure of oxidative status, as well as protein carbonylation as a measure of oxidative damage, in corkwing wrasse (Symphodus melops) captured near a disused copper mine, where water and sediment are contaminated with heavy metals, and an aluminum smelter, a site contaminated with PAHs. Results were compared to two different reference sites. Fish at the heavy metal site had lower glucose-6-phosphate dehydrogenase activity and elevated protein carbonyls (1.8 times) compared to fish from the reference site. At the PAH site, EROD was increased 2-fold, while total glutathione and methemoglobin reductase concentration, were decreased. No differences were seen in protein carbonyl levels at the PAH site. Measures of both antioxidant defenses and oxidative damage should be used when assessing effects of xenobiotics on oxidative stress in fish species.  相似文献   

Comparative oxygen radical formation and toxicity of BDE 47 in rainbow trout cell lines   总被引：2，自引：0，他引：2  

Shao J  Eckert ML  Lee LE  Gallagher EP 《Marine environmental research》2008,66(1):7-8

The polybrominated diphenyl ethers (PBDEs) constitute a class of flame retardants whose residues have markedly increased in fish and human tissues during the last decade. In particular, the levels of certain PBDE congeners in salmon have raised concern regarding potential risks associated with dietary PBDE exposures. However, little is known regarding PBDE-mediated cell injury in relevant in vitro cell models. We conducted a comparative study of oxyradical production and cell injury in rainbow trout gill (RTgill-W1) and trout liver cells (RTL-W1) exposed to 2,2',4,4'-tetrabromodiphenyl ether (BDE 47), a predominant BDE residue found in fish tissues such as salmonids. Exposure to low micromolar concentrations of BDE 47 elicited a significant loss in RTgill-W1 and RTL-W1 cell viability as measured by alamarBlue assay. The dose-response of BDE toxicity differed among the two cell lines, with the RTL-W1 liver cells showing greater resistance to toxicity at lower BDE 47 doses, but a more dramatic loss of viability relative to gill cells when challenged with higher (50 microM) doses. The sensitivity of the trout liver cells at higher BDE 47 exposures was reflected by a higher basal production of oxygen radical production by 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence that was markedly enhanced in the presence of BDE 47, suggesting an overwhelming of trout liver cell antioxidant defense pathways. Collectively, our data indicate that RTgill-W1 and RTL-W1 liver cells are sensitive to BDE 47-mediated cell injury through a mechanism that may involve oxidative stress. Our data also provide an in vitro basis for potential tissue differences in BDE 47-mediated cell injury.  相似文献   

东地中海对气候作用力的响应：来自最后两个间冰期高峰的认知     

Gianluca  Marino  Eelco  J.  Rohling  Francesca  Sanglorgi  Jaap  Sinninghe  Damste  Henk  Brinkhuis  陆红锋 《海洋地质》2008,24(3)

有关地中海海洋系统对气候效应的灵敏性这一问题仍处于争辩阶段。在这里,我们研究了在过去最后两个间冰期高峰期间东地中海海洋循环对日照效应的长期反应,在这期间大规模的水文变化导致了富含有机质的沉积物的沉积。  相似文献   

地中海东部泥火山结构中烃类气体的来源迁移以及在水体中的行为     

Vincent  Mastalerz  Anke  Dahlmann  Gert  J.  De  Lange  陆红锋 《海洋地质》2008,24(3)

在被动大陆边缘的陆地和海底环境,常常可以发现泥火山结构的存在。泥火山的喷发主要由于沉积物深部形成的高压所诱发,而这种高压主要是由过度的气体和水体压力的积聚引起。这种情况下,气体、水体和沉积物被活化,沿着沉积物（通常是断裂）向上迁移,形成泥穹丘。  相似文献   

Modeling Arctic Ocean heat transport and warming episodes in the 20th century caused by the intruding Atlantic Water     

Jun Takahashi  Tatsuo Suzuki  Igor V Polyakov  Kohei Mizobata  Moto Ikeda  Fancois J.Saucier  Markus Meier 《极地研究(英文版)》2008,19(2):159-167

This study investigates the Arctic Ocean warming episodes in the 20th century using both a high-resolution coupled global climate model and historical observations. The model, with no flux adjustment, reproduces well the Atlantic Water core temperature （AWCT） in the Arctic Ocean and shows that four largest decadalscale warming episodes occurred in the 1930s, 70s, 80s, and 90s, in agreement with the hydrographic observational data. The difference is that there was no pre-warming prior to the 1930s episode, while there were two pre-warming episodes in the 1970s and 80s prior to the 1990s, leading the 1990s into the largest and prolonged warming in the 20th century. Over the last century, the simulated heat transport via Fram Strait and the Barents Sea was estimated to be, on average, 31.32 TW and 14.82 TW, respectively, while the Bering Strait also provides 15.94 TW heat into the west- ern Arctic Ocean. Heat transport into the Arctic Ocean by the Atlantic Water via Fram Strait and the Barents Sea correlates significantly with AWCT （ C = 0.75 ） at 0- lag. The modeled North Atlantic Oscillation （NAO） index has a significant correlation with the heat transport （ C = 0.37 ）. The observed AWCT has a significant correlation with both the modeled AWCT （ C =0.49） and the heat transport （ C =0.41 ）. However, the modeled NAO index does not significantly correlate with either the observed AWCT （ C = 0.03 ） or modeled AWCT （ C = 0.16 ） at a zero-lag, indicating that the Arctic climate system is far more complex than expected.  相似文献   

Significance test for empirical orthogonal function (EOF) analysis of meteorological and oceanic data     

李晓峰  Pietrafesa  L.J  蓝淑芳  谢立安 《中国海洋湖沼学报》2000,18(1):10-17

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A New Emission Mode of PSR B1859+07     

Tao Wang  P.F.Wang  J.L.Han  Yi Yan  Ye-Zhao Yu  Feifei Kou 《天文和天体物理学研究(英文版)》2023,(10):178-185

Previous studies have identified two emission modes in PSR B1859+07: a normal mode that has three prominent components in the average profile, with the trailing one being the brightest, and an anomalous mode(i.e., the A mode) where emissions seem to be shifted to an earlier phase. Within the normal mode, further analysis has revealed the presence of two submodes, i.e., the cW mode and c B mode, where the central component can appear either weak or bright. As for the anomalous mode, a new bright ...  相似文献   

Challenges in the Detection and Attribution of Northern Hemisphere Surface Temperature Trends Since 1850     

Ronan Connolly  Willie Soon  Michael Connolly  Sallie Baliunas  Johan Berglund  C. J. Butler  Rodolfo Gustavo Cionco  Ana G. Elias  Valery M. Fedorov  Hermann Harde  Gregory W. Henry  Douglas V. Hoyt  Ole Humlum  David R. Legates  Nicola Scafetta  Jan-Erik Solheim  László Szarka  Víctor M. Velasco Herrera  Hong Yan  Weijia Zhang 《天文和天体物理学研究(英文版)》2023,(10):369-388

Since 2007, the Intergovernmental Panel on Climate Change(IPCC) has heavily relied on the comparison between global climate model hindcasts and global surface temperature(ST) estimates for concluding that post-1950s global warming is mostly human-caused. In Connolly et al., we cautioned that this approach to the detection and attribution of climate change was highly dependent on the choice of Total Solar Irradiance(TSI) and ST data sets.We compiled 16 TSI and five ST data sets and found by alter...  相似文献   

Effect of Matching Algorithm and Profile Shape on Pulsar Pulse Time of Arrival Uncertainties     

J.Wang  J.P.W.Verbiest  G.M.Shaifullah  J.P.Yuan 《天文和天体物理学研究(英文版)》2023,(12):232-243

For high-precision pulsar timing analysis and low-frequency gravitational wave detection, it is essential to accurately determine pulsar pulse times of arrival(ToAs) and associated uncertainties. To measure the ToAs and their uncertainties, various cross-correlation-based techniques can be employed. We develop methodologies to investigate the impact of the template-matching method, profile shape, signal-to-noise ratio of both template and observation on ToA uncertainties. These methodologies are...  相似文献   

Differential sensitivity of flounder (Platichthys flesus) in response to oestrogenic chemical exposure: an issue for design and interpretation of monitoring and research programmes     

Kirby MF  Smith AJ  Barry J  Katsiadaki I  Lyons B  Scott AP 《Marine environmental research》2006,62(4):315-325

This study was conducted as an initial investigation of 'differential response' in one of the main sentinel organisms used for monitoring programmes in United Kingdom estuaries, the flounder Platichthys flesus. It has been hypothesised that monitoring using species with a wide geographical spread and limited migration, such as flounder, might result in the comparison of different genetic stocks and certainly of populations with differing early life stage contaminant exposure histories. Furthermore, it is probable that these pre-exposure and genetic differences could manifest themselves in an ability to respond differently to contaminant exposure, so-called 'differential response'. It is important that the extent and nature of this response is understood, if we want to be able to fully interpret the monitoring data from such programmes. During this study, flounder were collected from four separate sources; wild caught fish from the estuaries of the Rivers Alde, Mersey and Tyne, and farmed flounder from Port Erin Farm, Isle of Man. Under controlled laboratory conditions, groups of fish from each source were exposed to water-borne concentrations of the synthetic oestrogen ethynylestradiol (EE2) at a nominal concentration of 50 ng/l. Plasma was taken from each male fish after 6 and 10 days exposure and analysed for the presence of vitellogenin (VTG) using an ELISA technique. Significant levels of VTG induction were evident in fish from all sources after both 6 and 10 days exposure. Flounder from the Mersey were the only fish with significantly elevated initial background levels of VTG (day 0) and this appeared to be reflected in that these specimens showed the highest induction response after day 6. However, after day 10, fish from all other sites had a slightly higher mean VTG than those from the Mersey which showed significantly (p < 0.05) lower mean plasma VTG. It is suggested that other differential responses may have been masked by the use of a high dose of EE2 which produced maximum induction in nearly all fish. The findings of the study are discussed in terms of implications for further research into the differential response issue and how the initial plasma VTG figures contribute to a time-series from the Mersey, Tyne and Alde estuaries.  相似文献