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131.
目的:研究卫矛醇对C6胶质瘤细胞株的体外增殖及凋亡的影响。方法:将C6大鼠胶质瘤细胞随机分为对照组、卫矛醇干预组、卫矛醇+雷帕霉素组、卫矛醇+3-MA组,各组予不同药物孵育,采用MTT法检测卫矛醇对C6细胞的体外增殖抑制率,筛选出抑制率相对较高且生长状态良好的细胞;应用Hoechest 33342染色透射电镜观察细胞凋亡亚显微结构的改变,采用免疫荧光和Western Blot技术检测与凋亡和自噬相关蛋白的表达水平。结果:卫矛醇对C6胶质瘤细胞增殖有明显的抑制作用,并呈现出良好的浓度-效应相关性,与对照组比较,随着浓度的增加,细胞的增殖抑制率上升(P<0.01);Hoechst染色结果显示,与对照组相比,共同孵育卫矛醇24 h后的C6细胞出现明显的核固缩现象,证明有大量的细胞出现凋亡;Western Blot结果显示,与对照组相比,促凋亡基因Bax表达上调,抗凋亡基因Bcl-2表达下调。结论:卫矛醇能够抑制C6细胞的增殖,并诱导其凋亡,其作用机制可能与上调Bax、下调Bcl-2有关。 相似文献
132.
针对城市土地资源变化检测工作繁杂、工作量大、自动化程度低等问题,本文提出一种基于深度学习模型的高分辨率遥感影像建筑物变化检测方法,将语义分割的思想引入到遥感变化检测。基于残差结构特征较卷积层提取性能更优和特征金字塔网络多尺度预测的特点,将残差结构和特征金字塔网络融合到Unet模型中,建立FPN Res-Unet模型。该模型以Unet为基础,引入ResNet18的残差结构作为编码路径特征提取层,在每次卷积后使用边界填充,使得输入图像和输出图像尺寸一致;在解码路径每级上采样过程中,拓展支路径将特征金字塔网络融合到模型的网络主干中,将残差结构、Unet及特征金字塔网络的优点相互融合,增强了Unet的特征提取,弥补了语义分割网络对小目标检测的欠缺;在获取深层语义信息的同时关注细节信息,提高建筑物变化检测精度。实验表明,该方法在所用数据集,准确率、召回率、F1 3种指标均达到90%以上。 相似文献
133.
"Using 1990 census data, this paper calculates the flow [of the foreign-born population in the United States] between states and from abroad; also, a multinomial logistic regression model of destination choice is estimated for resettlers and for migrants from abroad. There are three major findings. Firstly, Florida and California are the largest recipients of foreign-born resettlers; New York is the biggest loser of secondary migrants. Secondly, the presence of large communities of Mexicans in California and Cubans in Florida are very attractive to both resettlers and migrants from abroad. Thirdly, immigrants that are most in need of ethnic support networks (i.e. new arrivals or immigrants with low human capital resources) are most likely to choose one of the traditional immigration states as a destination." 相似文献
134.
135.
The extractable organic matter of 10 immature samples from a marl bed of one evaporitic cycle of the Vena del Gesso sediments (Gessoso-solfifera Fm., Messinian, Italy) was analyzed quantitatively for free hydrocarbons and organic sulphur compounds. Nickel boride was used as a desulphurizing agent to recover sulphur-bound lipids from the polar and asphaltene fractions. Carbon isotopic compositions (delta vs PDB) of free hydrocarbons and of S-bound hydrocarbons were also measured. Relationships between these carbon skeletons, precursor biolipids, and the organisms producing them could then be examined. Concentrations of S-bound lipids and free hydrocarbons and their delta values were plotted vs depth in the marl bed and the profiles were interpreted in terms of variations in source organisms, 13 C contents of the carbon source, and environmentally induced changes in isotopic fractionation. The overall range of delta values measured was 24.7%, from -11.6% for a component derived from green sulphur bacteria (Chlorobiaceae) to -36.3% for a lipid derived from purple sulphur bacteria (Chromatiaceae). Deconvolution of mixtures of components deriving from multiple sources (green and purple sulphur bacteria, coccolithophorids, microalgae and higher plants) was sometimes possible because both quantitative and isotopic data were available and because either the free or S-bound pool sometimes appeared to contain material from a single source. Several free n-alkanes and S-bound lipids appeared to be specific products of upper-water-column primary producers (i.e. algae and cyanobacteria). Others derived from anaerobic photoautotrophs and from heterotrophic protozoa (ciliates), which apparently fed partly on Chlorobiaceae. Four groups of n-alkanes produced by algae or cyanobacteria were also recognized based on systematic variations of abundance and isotopic composition with depth. For hydrocarbons probably derived from microalgae, isotopic variations are well correlated with those of total organic carbon. A resistant aliphatic biomacromolecule produced by microalgae is, therefore, probably an important component of the kerogen. These variations reflect changes in the depositional environment and early diagenetic transformations. Changes in the concentrations of S-bound lipids induced by variations in conditions favourable for sulphurization were discriminated from those related to variations in primary producer assemblages. The water column of the lagoonal basin was stratified and photic zone anoxia occurred during the early and middle stages of marl deposition. During the last stage of the marl deposition the stratification collapsed due to a significant shallowing of the water column. Contributions from anaerobic photoautotrophs were apparently associated with variations in depth of the chemocline. 相似文献
136.
我国地方性氟病的地球化学问题 总被引:18,自引:0,他引:18
Endemic fluorine disease is a chronic fluorine poisoning caused by high level of fluorine in drinking water or foods. Incidence of this disease has been knowu in many parts of the world. In China its occurrence has been observed in the Northeast, Northwest, Southwest and some other localities. Investigation shows that it is closely related to geochemical environment: Major fluorine sources in nature include fluorinerich volcanic rocks, calcareous dolomites, phosphorus-bearing rocks/loess and Quarternary loose sediments. Under special conditions fluorine is concentrated in ground water or soil and seeks its way into human body through diet. Areas With high fluorine concentration responsible for the fluorine disease can be classified into four types, depending on characteristics of geochemical environment: (1) Arid and semiarid calcium-rich soda saline areas; (2) Semiarid, alternatively marine-terrestrial strata with a high degree of salinity; (3) Semihumid iron- and aluminum-rich intermediate-acid soils; (4) Localities where fluorine-rich rocks or deposits are developed;and those with high fluorine content as a result of industrial pollution. Based on medical statistics and chemical analyses, of water, soil, food and other related samplcs,this work will provide a scientific baekground for the prevention and control of fluorine disease. 相似文献
137.
声发射技术在岩石力学研究中的应用 总被引:19,自引:0,他引:19
随着地震预报研究的开展,岩石力学与地震预报的联系越来越紧密。声发射技术是岩石力学研究中十分重要的工具之一。本文对声发射技术作了简单的介绍,评述了它在岩石力学研究中的应用,介绍了用声发射技术研究地震序列和破裂机制的一些结果。 相似文献
138.
Diploptene: an indicator of terrigenous organic carbon in Washington coastal sediments 总被引:1,自引:0,他引:1
The pentacyclic triterpene 17 beta(H),21 beta(H)-hop-22(29)-ene (diploptene) occurs in sediments throughout the Columbia River drainage basin and off the southern coast of Washington state in concentrations comparable to long-chain plantwax n-alkanes. The same relationship is evident for diploptene and long-chain n-alkanes in soils from the Willamette Valley. Microorganisms indigenous to soils and soil erosion are indicated as the biological source and physical process, respectively, for diploptene in coastal sediments. Similarity between the stable carbon isotopic composition (delta 13CPDB) of diploptene isolated from soil in the Willamette Valley (-31.2 +/- 0.3%) and from sediments deposited throughout the Washington coastal environment (-31.2 +/- 0.5%) supports this argument. Values of delta for diploptene in river sediments are variable and 8-17% lighter, indicating that an additional biological source such as methane-oxidizing bacteria makes a significant contribution to the diploptene record in river sediments. Selective biodegradation resulting from a difference in the physicochemical association within eroded particles can explain the absence of the more-13C-depleted form of diploptene in Washington coastal sediments, but this mechanism remains unproven. 相似文献
139.
Reports of the 13C content of marine particulate organic carbon are compiled and on the basis of GEOSECS data and temperatures, concentrations, and isotopic compositions of dissolved CO2 in the waters in which the related phytoplankton grew are estimated. In this way, the fractionation of carbon isotopes during photosynthetic fixation of CO2 is found to be significantly correlated with concentrations of dissolved CO2. Because ancient carbon isotopic fractionations have been determined from analyses of sedimentary porphyrins [Popp et al., 1989], the relationship between isotopic fractionation and concentrations of dissolved CO2 developed here can be employed to estimate concentrations of CO2 dissolved in ancient oceans and, in turn, partial pressures of CO2 in ancient atmospheres. The calculations take into account the temperature dependence of chemical and isotopic equilibria in the dissolved-inorganic-carbon system and of air-sea equilibria. Paleoenvironmental temperatures for each sample are estimated from reconstructions of paleogeography, latitudinal temperature gradients, and secular changes in low-latitude sea surface temperature. It is estimated that atmospheric partial pressures of CO2 were over 1000 micro atm 160 - 100 Ma ago, then declined to values near 300 micro atm during the next 100 Ma. Analysis of a high-resolution record of carbon isotopic fractionation at the Cenomanian-Turonian boundary suggests that the partial pressure of CO2 in the atmosphere was drawn down from values near 840 micro atm to values near 700 micro atm during the anoxic event. 相似文献
140.
中国大陆架海底沉积物中的稀土元素 总被引:32,自引:2,他引:30
选择渤海、黄海、东海和南海有代表性的大陆架沉积物样品,利用X射张荧光光谱法测定了15个稀土元素。中国大陆架沉积物稀土元素的丰度为156ppm,相对接近于中国黄土和花岗岩的丰度,而与太平洋沉积物的丰度相差较远,具有明显的“亲陆性”。稀土的配分模式旦显著的负斜率,属陆壳稀土的典型特征。稀土含量受沉积物粒度控制。实验表明稀土主要以类质同像存在于粘土矿物之晶体构造中。根据数理统计,稀土与亲陆源粘土矿物的元素为一类,彼此呈正相关;与亲海洋生物的元素为负相关。事实说明中国大陆岩石的风化和搬运,是中国海大陆架沉积物中稀土的主要物质来源。 相似文献