Identification of genes expressed in juvenile Haliotis rufescens in response to different copper concentrations in the north of Chile under controlled conditions     

Silva-Aciares F  Zapata M  Tournois J  Moraga D  Riquelme C 《Marine pollution bulletin》2011,62(12):2671-2680

This study reports molecular markers potentially associated with resistance or sensitivity to the impact of copper in juvenile red abalone, Haliotis rufescens, in the north of Chile under experimental conditions. Genomic analysis was made applying subtractive hybridization libraries (SSH) to identify genes up-and down regulated during cooper exposure in abalone over periods of 12 and 168 h exposed to 2.5 and 10 μg/L of Cu⁺². Results obtained from the SSH library revealed 368 different sequences regulated by copper, that correspond to eight major physiological functions. The validation of these sequences obtained by SSH as well as their expression kinetics were made by PCR in real time on 14 potential genes regulated by metal stress. This study provides information for the characterization of potential genomic markers that may be used in future environmental monitoring and to investigate new mechanisms of stress to copper in this commercially important marine species.  相似文献   

Validation of Arenicola marina in field toxicity bioassays using benthic cages: biomarkers as tools for assessing sediment quality     

Ramos-Gómez J  Martins M  Raimundo J  Vale C  Martín-Díaz ML  DelValls TA 《Marine pollution bulletin》2011,62(7):1538-1549

Sediment toxicity assessments using caged organisms present advantages over using laboratory and native community studies. The use of caged Arenicola marina in sediment toxicity assessments was evaluated. Lugworms were exposed in situ to sediments from coastal and port areas in Spain for seven days, and the activities of the biotransformation enzymes ethoxyresorufin O-deethylase, dibenzylfluorescein dealkylase and glutathione S-transferase, the activities of the antioxidant enzymes glutathione reductase and glutathione peroxidase and lipid peroxidation were then analyzed as biomarkers. Biomarker results and sediment physicochemical data were integrated. Cádiz Bay (SW Spain) sediments presented metal contamination that was not linked to a biochemical response. In LPGC Port (SW Spain), Pb contamination exhibited a moderate toxic potential, while PAHs, and presumably pharmaceuticals, provoked biochemical responses that efficiently prevented lipid peroxidation. In Santander Bay (N Spain), exposure to PAHs and, presumably, pharmaceuticals induced biomarker responses, but lipid peroxidation occurred nevertheless. These results indicated that caged A. marina were effective for the assessment of sediment quality and that the selected biomarkers were sufficiently sensitive to identify chemical exposure and toxicity.  相似文献   

Assessment of site-effects using acceleration time series. Application to São Sebastião volcanic crater     

Jaime A. Santos  Pedro Chitas  Isabel Lopes  Carlos Sousa Oliveira  Isabel Moitinho de Almeida  João Carlos Nunes 《Soil Dynamics and Earthquake Engineering》2011

The Azores archipelago is located at the North America, Eurasia and Africa triple junction and shows seismicity and volcanism typical of an active plate boundary. Terceira Island has been affected by several damaging earthquakes being the 1980 January 1st event (M=7.2) the strongest in Azores during the last century.  相似文献   

Complex size-dependent habitat associations in potamodromous fish species     

José Maria Santos  Luís Reino  João Oliveira  Paulo Pinheiro  Pedro Raposo Almeida  Rui Cortes  Maria Teresa Ferreira 《Aquatic Sciences - Research Across Boundaries》2011,73(2):233-245

Knowledge of the distribution of species life stages at multiple spatial scales is fundamental to both a proper assessment of species management and conservation programmes and the ability to predict the consequences of human disturbances for river systems. The habitat requirements of three native cyprinid species—the Iberian barbel Barbus bocagei Steindachner, the Iberian straight-mouth nase Pseudochondrostoma polylepis (Steindachner), and the Northern straight-mouth nase Pseudochondrostoma duriense (Coelho)—were examined at 174 undisturbed or minimally disturbed sites in 8 river catchments across western Iberia, by modelling occurrence and counts of species life stages at two spatial scales—large (regional) and instream (local)—using hurdle models. All the life stages of the barbel showed a negative association with upstream high-gradient river reaches, whereas juvenile P. duriense favoured such areas. Stream width and openness were negatively related with the occurrence of juvenile and small adult barbel, but not with large adults. Juvenile nase, on the other hand, were found to be mainly confined to fast-flowing habitats with high instream cover and coarser substrata. Advanced life stages of the barbel were mainly associated with the “pure” regional and shared components, whereas the purely local attributes accounted for much of the model variation among nases, in particular juveniles, and juvenile barbel. The results of this study are useful for setting or refining management goals, and highlight the need to separately consider life stages when performing conservation-related studies of species distribution.  相似文献   

Ammonium,nitrate and phytoplankton interactions in a freshwater tidal estuarine zone: potential effects of cultural eutrophication   总被引：2，自引：0，他引：2  

Rita B. Domingues  Ana B. Barbosa  Ulrich Sommer  Helena M. Galvão 《Aquatic Sciences - Research Across Boundaries》2011,73(3):331-343

Nitrate and ammonium are the most important nitrogen sources for phytoplankton growth. Differential utilization of inorganic nitrogenous compounds by phytoplankton has been observed and may have significant impacts on primary productivity at local scales. We used enrichment experiments with natural phytoplankton populations from the freshwater tidal zone of the Guadiana estuary, a coastal ecosystem increasingly subjected to anthropogenic influences, to study the effects of nitrate and ammonium on N-consumption and phytoplankton growth. In addition, we used combined additions of nitrate and ammonium to understand the inhibitory effect of ammonium over nitrate uptake. Ammonium concentrations in the freshwater tidal reaches of the Guadiana estuary throughout the sampling period were too low to exert an inhibitory effect on nitrate uptake or a toxic effect on phytoplankton growth. Nitrate was clearly the main nitrogen source for phytoplankton at the study site. Overall, nitrate seemed to become limiting at concentrations lower than 20 μM and N-limitation was particularly significant during summer. A trend of decreasing nitrate uptake with increasing ammonium concentrations and uptake suggested an overall preference for ammonium. However, preference for ammonium was group-specific, and it was observed mainly in green algae and cyanobacteria. In fact, cyanobacteria relied only on ammonium as their N-source. On the contrary, diatoms preferred nitrate, and did not respond to ammonium additions. The increasing eutrophication in the Guadiana estuary and particularly increased inputs of nitrogen as ammonium due to urban waste effluents may result in a shift in phytoplankton community composition, towards a dominance of cyanobacteria and green algae.  相似文献   

Active surface faulting or landsliding in the Lower Tagus Valley (Portugal)? A solved controversy concerning the Vila Chã de Ourique site     

João Manuel Cabral  Fernando Marques  Paula Figueiredo  Luís Matias 《Journal of Seismology》2011,15(2):215-234

The Lower Tagus Valley has experienced significant (M 6–7) historical seismicity, evidencing the presence of seismogenic faults. These are still deficiently known due to the low strain rates and the recent alluvial sedimentation of the Tagus River that buries most of the structures, though Paleoseismic evidence was allegedly found by a research team in the Tagus valley, at a site 60 km N of Lisbon, near Vila Chã de Ourique (VCO). According to this team, trenching at the VCO site exposed an active thrust fault, evidencing the surface rupture of a large earthquake that occurred in 1531. Our studies performed at this site, comprising field observations with a reappraisal of the trench outcrops previously excavated, borehole drilling, soil mechanics laboratory testing, and seismic reflection acquisition, pointed to the alternative interpretation that the outcropping structures are gravitational and not of tectonic origin. The interpretation of new outcrops crosscutting the structures exposed at the trenches, as well as newly acquired high-resolution seismic reflection data, definitely exclude the active thrust fault explanation and support a gravitational slip model for all the observed structures. Gravitational slip in the river bank slope was promoted by low shear strength clays and high pore water pressure coupled with slope toe river erosion. Gravitational slides must have occurred prior to development of the present sedimentation level of the Tagus alluvial plain, which was attained in the last few thousand years as indicated by borehole data and estimations of sedimentation rates.  相似文献   

Experimental study of friction dissipators for seismic protection of building structures   总被引：1，自引：1，他引：0  

F. López-Almansa  S.T. de la Cruz  C. Taylor 《地震工程与工程振动(英文版)》2011,10(4):475-486

This paper presents the results from unidirectional shaking table tests of two reduced scale steel models of a building frame, with one and two floors, respectively. These frames incorporate friction dissipators at every floor. The inputs are sine-dwells and artificial and registered earthquakes. This study is part of a larger research project aiming to assess the seismic efficiency of friction dissipators by means of an integrated numerical and experimental approach. Inside this framework, the main objectives of these experiments are to: (i) collect a wide range of results to calibrate a numerical model derived within the project, (ii) clarify some of the most controversial issues about friction dissipators (including behavior for inputs containing pulses, capacity to reduce resonance peaks, introduction of high frequencies in the response, and self-generated eccentricities), (iii) better understand their dynamic behavior, (iv) provide insight on the feasibility and reliability of using simple friction dissipators for seismic protection of building structures and (v) characterize the hysteretic behavior of these devices. Most of these objectives are satisfactorily reached and relevant conclusions are stated.  相似文献   

An Environmental Threshold of Soil Test P and Degree of P Saturation of Brazilian Oxisols     

Amlan Kumar Ghosh  Joyce Barbosa  Ivo Ribeiro da Silva 《洁净——土壤、空气、水》2011,39(5):421-427

To develop P management guidelines for poultry litter application in Oxisols of Brazil, the relationship between water soluble P (WSP), soil test P (STP), and degree of P saturation (DPS) needs to be established. The study examined this relationship in Oxisols of Brazil receiving poultry litter. Soils were analyzed for Mehlich‐1 P (M1‐P), WSP, P fractions, P sorption index (PSI), and P saturation based on Mehlich‐1 extractable P, Al, Fe (DPS_M1), and PSI (DPS_PSI). Surface water bodies in the catchment area were examined for inorganic and total P. All the surface water bodies in the catchment area of the sampled plots were highly enriched in inorganic and organic P. Enrichment of soil P fractions following poultry litter application followed the order HCl‐bound > NaOH‐bound > NaHCO₃‐bound fraction. M1‐P was correlated to labile (NaHCO₃‐P_i), and stable P pools (NaOH‐P and HCl‐P) and reflected the cumulative P accumulation in these soils. P saturation indices increased with cumulative P addition and increasing M1‐P. WSP increased with increasing DPS and a change point was noticed at 23% DPS_PSI (equivalent to DPS_M1 of 16.5%) corresponding to M1‐P of 44.5 mg P kg^?1, which could be regarded as threshold for P loss in these soils.  相似文献   

On‐line Solid Phase Extraction of Copper in Water Samples with Flow Injection Flame Atomic Absorption Spectrometry     

Tülin Çetin  Ahmet Ülgen  Şerife Tokalıoğlu 《洁净——土壤、空气、水》2011,39(3):244-249

An on‐line solid phase extraction method for the preconcentration and determination of Cu(II) by flame atomic absorption spectrometry has been described. The procedure is based on the retention of Cu(II) ions at pH 6.0 on a minicolumn packed with Amberlite XAD‐1180 resin impregnated with chrome azurol S. After preconcentration, Cu(II) ions adsorbed on the impregnated resin were eluted by 1 mol L^?1 HNO₃ solution. Several parameters, such as pH, type of eluent, flow rates of sample and eluent solutions, amount of resin were evaluated. At optimized conditions, for 3.5 min of preconcentration time, the system achieved a detection limit of 1.0 µg L^?1, and a relative standard deviation of 1.2% at 0.2 µg mL^?1 copper. An enrichment factor of 56‐fold was obtained with respect to the copper determination. The proposed method was successfully validated by the analysis of standard reference material (TMDA 54.4 lake water) and recovery studies. The method was applied to the preconcentration of Cu(II) in natural water samples.  相似文献   

The Mid-Rivera-Transform Discordance: Morphology and Tectonic Development     

William L. Bandy  Fran?ois Michaud  Carlos A. Mortera Guti��rrez  J��r?me Dyment  Jacques Bourgois  Jean-Yves Royer  Thierry Calmus  Marc Sosson  Jose Ortega-Ramirez 《Pure and Applied Geophysics》2011,168(8-9):1391-1413

To better define the morphotectonic elements and tectonic development of the Mid-Rivera-Transform Discordance, multibeam bathymetric, seafloor backscatter, multichannel seismic reflection and total field marine magnetic data were collected along the entire Rivera Transform west of 107°W during the BART and FAMEX campaigns of the N.O. L??Atalante conducted in 2002. These data show that, although the transform tectonized zone of the Rivera Transform west of 107°30??W is a single continuous morphologic basin, this basin consists of two distinct morphotectonic domains: an eastern domain which morphologically is a deep rhombochasm within which organized seafloor spreading has occurred, and a western ??leaky transform?? domain. These new data, in conjunction with the results of previous studies, support the idea that the Rivera-Pacific Euler pole is migrating southward towards the eastern half of the Rivera Transform, and further indicate a recent (<0.14?Ma), and most likely ongoing, clockwise reorganization of the principle transform displacement zones of the Rivera Transform west of 108°W. We propose that the Mid-Rivera-Transform Discordance owes its origin to this eastward progressing, clockwise reorganization of the transform segments that is occurring in response to recent changes in Rivera-Pacific relative plate motion.  相似文献