A new method to detect viable bacteria in natural seawater using 16SrRNA oligonucleotide probe     

Masahiko Nishimura  Kumiko Kita-Tsukamoto  Kazuhiro Kogure  Kouichi Ohwada  Usio Simidu 《Journal of Oceanography》1993,49(1):51-56

Fluorescent oligonucleotide probes were used to detectVibrio cholerae directly under epifluorescence microscope. The probe which is complementary to the specific 16SrRNA sequence ofVibrio cholerae was labelled with Texas-Red, whereas the universal probe was labelled with Fluorescein. These probes allowed the distinctionVibrio cholerae from other eubacteria under the same microscopic field. In order to detect and enumerate specific bacteria in natural seawater, this method was combined with the direct viable count (DVC) technique. The combined method increased intracellular rRNA levels in the sample, and made it possible to detect the target bacteria with the specific gene probe. The applicability of this new method was confirmed both for the laboratory mixed culture system and natural seawater.  相似文献   

Seasonal Changes in Oceanic pCO2 in the Oyashio Region from Winter to Spring     

Takashi Midorikawa  Sonoki Iwano  Kazuhiro Saito  Hiroyuki Takano  Hitomi Kamiya  Masao Ishii  Hisayuki Y. Inoue 《Journal of Oceanography》2003,59(6):871-882

We observed the partial pressure of oceanic CO₂, pCO₂ ^sea, and related surface properties in the westernmost region of the subarctic North Pacific, seasonally from 1998 to 2001. The pCO₂ ^sea in the Oyashio region showed a large decrease from winter to spring. In winter, pCO₂ ^sea was higher than 400 μatm in the Oyashio region and this region was a source of atmospheric CO₂. In spring, pCO₂ ^sea decreased to extremely low values, less than 200 μatm (minimum, 139 μatm in 2001), around the Oyashio region with low surface salinity and this region turned out to be a strong sink. The spatial variations of pCO₂ ^sea were especially large in spring in this region. The typical Oyashio water with minimal mixing with subtropical warm water was extracted based on the criterion of potential alkalinity. The contribution of main oceanic processes to the changes in pCO₂ ^sea from winter to spring was estimated from the changes in the concentrations of dissolved inorganic carbon and nutrients, total alkalinity, temperature and salinity observed in surface waters in respective years. These quantifications indicated that photosynthesis made the largest contribution to the observed pCO₂ ^sea decreases in all years and its magnitude was variable year by year. These year-to-year differences in spring biological contribution could be linked to those in the development of the density stratification due to the decrease in surface salinity. Thus, the changes in the surface physical structure could induce those in pCO₂ ^sea in the Oyashio region in spring. Furthermore, it is suggested that the direction and magnitude of the air-sea CO₂ flux during this season could be controlled significantly by the onset time of the spring bloom. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Preface     

Yoshihisa Shirayama  Kazuhiro Kogure  Peter G. Brewer  Takashi Ohsumi  Joji Ishizaka  Jun Kita  Yuji Watanabe 《Journal of Oceanography》2004,60(4):691-691

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Local equilibrium during the contact metamorphism of siliceous dolomites in Kasuga-mura,Gifu-ken,Japan     

Kazuhiro Suzuki 《Contributions to Mineralogy and Petrology》1977,65(1):79-89

Metamorphism to intermediate-pressure granulite grade had a minimal effect on the geochemistry of layered gneisses in central Australia. The overall composition of the terrain is granodioritic and major element compositions have equivalents in igneous and sedimentary supracrustal rocks. K, Rb, Sr and probably Th concentrations, and K/Rb ratios are normal; the initial isotopic composition of Sr shows the usual range of crustal rocks. However, U is strongly depleted and was lost by a pervasive process, probably dehydration, rather than by anatexis. Comparison with other areas in which major chemical depletions and unusually low initial Sr isotopic ratios are postulated leads to alternative interpretations of these areas which do not involve large scale chemical migration. An intermediate composition for the lower crust may result from a high density of basic intrusions rather than chemical processes.  相似文献   

Preface     

Atsushi Tsuda  Kazuhiro Kogure  Shuichi Watanabe 《Journal of Oceanography》2016,72(1):1-2

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Anomalously shallow inclination in middle–northern part of the South China block: palaeomagnetic study of Late Cretaceous red beds from Yichang area     

Kazutoshi Narumoto  Zhenyu Yang  Kazuhiro Takemoto  Haider Zaman  Hayao Morinaga  Yo-ichiro Otofuji 《Geophysical Journal International》2006,164(2):290-300

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Long-term changes in distribution and chemistry of middle Miocene to Quaternary volcanism in the Chokai-Kurikoma area across the Northeast Japan Arc   总被引：1，自引：0，他引：1  

Hirofumi Kondo  Kazuhiro Tanaka  Yukihiro Mizuochi  Atusi Ninomiya 《Island Arc》2004,13(1):18-46

Abstract To understand the characteristics of long‐term spatial and temporal variation in volcanism within a volcanic arc undergoing constant subduction since the cessation of back‐arc opening, a detailed investigation of middle Miocene to Quaternary volcanism was carried out within the Chokai‐Kurikoma area of the Northeast Japan Arc. This study involved a survey of available literature, with new K–Ar and fission track dating, and chemical analyses. Since 14 Ma, volcanism has occurred within the Chokai‐Kurikoma area in specific areas with a ‘branch‐like’ pattern, showing an east–west trend. This is in marked contrast to the widespread distribution of volcanism with a north–south trend in the 20–14 Ma period. The east–west‐ trending ‘branches’ are characterized by regular intervals (50–100 km) of magmatism along the arc. These branches since 14 Ma are remarkably discrepant to the general northwest–southeast or north‐northeast–south‐southwest direction of the crustal structures that have controlled Neogene to Quaternary tectonic movements in northeast Japan. In addition, evidence indicating clustering and focusing of volcanism into smaller regions since 14 Ma was verified. Comparison of the distribution and chemistry of volcanic rocks for three principal volcanic stages (11–8, 6–3 and 2–0 Ma) revealed that widely but sparsely distributed volcanic rocks had almost the same level of alkali and incompatible element concentrations throughout the area (with the exception of Zr) in the 11–8 Ma stage. However, through the 6–3 Ma stage to the 2–0 Ma stage, the concentration level in the back‐arc cluster increased, while that in the volcanic front cluster remained almost constant. Therefore, the degree of partial melting has decreased, most likely with a simultaneous increase in the depth of magma segregation within the back‐arc zone, whereas within the volcanic front zone, the conditions of magma generation have changed little over the three stages. In conclusion, the evolution of the thermal structure within the mantle wedge across the arc since 14 Ma has reduced the extent of ascending mantle diapirs into smaller fields. This has resulted in the tendency for the distribution of volcanism to become localized and concentrated into more specific areas in the form of clusters from the late Miocene to Quaternary.  相似文献   

Early Cretaceous tectonic events implied in the time-lag between the age of radiolarian chert and its metamorphic basement in the Bantimala area, South Sulawesi, Indonesia     

Koji  Wakita  Munasri  Jan  Sopaheluwakan  Iskandar  Zulkarnain Kazuhiro  Mlyazaki 《Island Arc》1994,3(2):90-102

Abstract The Bantimala Complex of South Sulawesi consists mainly of mélange, chert, basalt, ultramafic rocks and high pressure type metamorphic rocks. Well-preserved radiolarians were extracted from 10 samples of chert, and K-Ar age dating was done for muscovite from five samples of schist of the Bantimala Complex. The radiolarian assemblage from chert is assigned middle Cretaceous (late Albian-early Cenomanian) age, while the K-Ar age data from schist range from 132 Ma to 114 Ma except for one sample with rare muscovite. The radiolarian chert is unconformably underlain by schist in the Bantimala Complex. The stratigraphie relationship and the time lag of these two kinds of age data from chert and underlying schist suggest short-time tectonic events immediately followed by a quick waning tectonism in this region during the Albian-Cenomanian transgression.  相似文献   

The chemical Th-U-total Pb isochron ages of Jiaodong and Jiaonan metamorphic rocks in the Shandong Peninsula, eastern China   总被引：3，自引：0，他引：3  

Masaki  Enami  Kazuhiro  Suzuki  Mingguo  Zhai Xiangshen  Zheng 《Island Arc》1993,2(2):104-113

Abstract The chemical Th-U-total Pb isochron method (CHIME) was applied to determine the age of monazite and thorite in five gneisses and zircon in an ultra high-pressure (UHP) phengite schist from the Su-Lu region, eastern China. The CHIME ages and isotopic ages reported in the literature show that gneisses in the Su-Lu region are divided into middle Proterozoic (1500–1720 Ma) and Mesozoic (100–250 Ma) groups. The Proterozoic group includes paragneiss and orthogneiss of the amphibolite-granulite facies, and their protolith age is late Archean-early Proterozoic. The Mesozoic group is mainly composed of orthogneiss of the greenschist-epidote amphibolite facies, and the protolith age is Middle Paleozoic-Early Proterozoic. The Proterozoic and Mesozoic gneisses occupy northern and southern areas of the Su-Lu region, respectively, which are divided by a major Wulian-Qingdao-Yantai fault. Ultra high-pressure metamorphic rocks occur as blocks in the Mesozoic gneisses, and form a UHP complex.
The UHP phengite schist in the Mesozoic orthogneiss contains detrital zircons with late Proterozoic CHIME age ( ca 860 Ma). Age of the UHP metamorphism is late Proterozoic or younger, and protolith age of the UHP metamorphic rocks is probably different from that of the surrounding Mesozoic gneisses.  相似文献   

Redistribution and export of contaminated sediment within eastern Fukushima Prefecture due to typhoon flooding          下载免费PDF全文

Akihiro Kitamura  Hiroshi Kurikami  Kazuyuki Sakuma  Alex Malins  Masahiko Okumura  Masahiko Machida  Koji Mori  Kazuhiro Tada  Yasuhiro Tawara  Takamaru Kobayashi  Takafumi Yoshida  Hiroyuki Tosaka 《地球表面变化过程与地形》2016,41(12):1708-1726

Tropical cyclones expose river basins to heavy rainfall and flooding, and cause substantial soil erosion and sediment transport. There is heightened interest in the effects of typhoon floods on river basins in northeast Japan, as the migration of radiocaesium‐bearing soils contaminated by the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident will affect future radiation levels. The five main catchments surrounding FDNPP are the Odaka, Ukedo, Maeda, Kuma and Tomioka basins, but little quantitative modelling has been undertaken to identify the sediment redistribution patterns and controlling processes across these basins. Here we address this issue and report catchment‐scale modelling of the five basins using the GETFLOWS simulation code. The three‐dimensional (3D) models of the basins incorporated details of the geology, soil type, land cover, and used data from meteorological records as inputs. The simulation results were checked against field monitoring data for water flow rates, suspended sediment concentrations and accumulated sediment erosion and deposition. The results show that the majority of annual sediment migration in the basins occurs over storm periods, thus making typhoons the main vectors for redistribution. The Ukedo and Tomioka basins are the most important basins in the region in terms of overall sediment transport, followed by the other three basins each with similar discharge amounts. Erosion is strongly correlated with the underlying geology and the surface topography in the study area. A low permeability Pliocene Dainenji formation in the coastal area causes high surface water flow rates and soil erosion. Conversely, erosion is lower in an area with high permeability granite basement rocks between the Hatagawa and Futaba faults in the centre of the study area. Land cover is also a factor controlling differences in erosion and transport rates between forested areas in the west of the study area and predominantly agricultural areas towards the east. The largest sediment depositions occur in the Ogaki and Takigawa Dams, at the confluence of the Takase and Ukedo Rivers, and at the Ukedo River mouth. Having clarified the sediment redistribution patterns and controlling processes, these results can assist the ongoing task of monitoring radioactive caesium redistribution within Fukushima Prefecture, and contribute to the design and implementation of measures to protect health and the environment. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献